TrcrB.19530.a

J-binding protein 1 (JBP1)

CENTER ID:	TrcrB.19530.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19530.a.B1.GE41522	full length		1	831
TrcrB.19530.a.B2.GE41523	Tet-JBP oxygenase		41	304

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79418

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.506753.120
UniProt:	Q4DLX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKQKRGKQDV KMVESAPPQL LPKKGRLEIS ELAPQQRTIR TAEEIEMAYN EAVRKHPFYD NADHTIDFHD ATVFRDARGV VGGVLLPGAL PAFAATMAAD VLRPAAVRTS LRSNMFGGFA PLSGIAGYFD YRGSPVELKC RKTSFTYENV HSWPNVFPMI DYVSAIYKAV FPERWAAQDA AVPDIVRIHG SPFSTLTVNQ QFRTASHTDA GDFDMGYGLL AVLEGKFEGL SLALDDFGVC FRMQPRDVLI FNTHFFHSNT EPELNHPKDD WSRLTCVCYY RAALGEPACV AEYERRLARA KEIGASPPPA VDAILQKDNG NNFNKPAPTF TYSLTPFGGA ASICSLHCCT AKLLRLHELL LENPTLEVIL FGESLRTDDG LPRREKEQLI SVHLPVVVKM SPSGGFSELG GALKAAEEKQ YFFEEKYLAD ELGPDLMSMW TQSRAHWLRL VKEDWERLCR RDPERTKFTW NNSSAMNAAF FDLCEVAKQM MIGLLNKETP SSAENHSFWI LFAAHLNYAC TTENGMPRDA VGMHKLNVKL KDFHFGGTRY LKDMPPEEQE RRLERKKRIE EARRRGNAAR ETHTDNWLLN DTFDYQQEDR KVEFEENGWM TPEAYVKHLG LKPCGDVTAA ASPTEPIHVL VVLPRPAAAA PKDVKRDVPL ATSEESIRLL MNPAAQRVLT GKARNVTLPS PLSFGGVKIT VLFDGDDIDC IHPDFVVLQH LLAAIEEDEA AKARVKYWAH VARYCVFVVE TDVRDRRHFL LREEVRVAYE DVAEDCFRSL HAAAYSTKCN RLRTTPSLIA LSNSKNIGLR FKFRGSPLNT IALIVVGERL D

NT Sequence

atgaaacaaa agagaggaaa gcaagacgtg aaaatggttg aatcagctcc tccacaactg ctcccaaaaa agggtcggct tgagatttct gaacttgctc cacaacaacg gacaatacgt actgcggaag agattgaaat ggcatacaat gaggccgtta gaaaacatcc tttttacgat aacgccgatc acacgattga ttttcatgac gccactgtat tccgtgacgc cagaggggta gtcgggggcg tcttgttacc aggggcgctg cctgcatttg ctgccaccat ggcagccgat gtgttgcgac ctgcagctgt aaggacatct ttacgatcga atatgtttgg tgggttcgca cctcttagtg gtatcgccgg ttactttgac tatcgcggat ctccagtgga actcaaatgc cgcaagacat cctttacata tgaaaacgtt cattcctggc caaatgtgtt tccgatgatt gattacgtca gtgccattta caaagctgtt ttcccggaac ggtgggccgc gcaggatgca gccgtgccgg atattgtccg cattcacgga tccccctttt ccaccttgac ggtgaatcaa cagtttcgga ccgcctcaca cacggatgcg ggtgattttg atatgggata cggtcttctg gcggttctgg agggcaaatt tgaaggtctc tccctcgcgt tggatgactt tggtgtctgc ttccgcatgc agccaaggga cgtcttgatt tttaacacgc acttctttca ttcgaataca gagccggagt tgaatcatcc aaaggacgac tggagtcgac taacctgtgt ttgctattat agggcggcgt tgggggaacc ggcgtgtgtt gcagaatatg aacgacgttt ggcgcgggct aaagaaatag gtgcctcccc ccctcctgct gtagatgcca ttttacaaaa ggacaatggc aacaatttta acaaacccgc accgacgttc acttattcac tcacaccgtt tggaggagcg gcgagcattt gctctctgca ttgctgcacc gccaaactcc tgcggctaca cgagctactg ttagaaaacc caacgttgga ggttatttta tttggagaat cgctacgaac ggacgatggc ctgcccagac gtgaaaagga gcaacttatt tctgtacacc ttcctgtggt tgtaaagatg tctccctccg gtgggtttag tgaattggga ggggcgctaa aggctgccga ggagaagcag tacttctttg aggaaaaata cttggcggat gaactcggtc cagacctcat gtccatgtgg acgcagagtc gtgctcactg gctgaggctc gtcaaggagg attgggagcg tctctgtcga agggatccag aacggacaaa atttacctgg aacaattcgt ctgcgatgaa tgcggccttt tttgatctct gtgaggtggc aaagcagatg atgattggat tgctaaacaa ggaaacacca tcatctgctg agaaccactc cttttggatt ctttttgcgg cacatttaaa ttacgcatgc accactgaaa atggcatgcc gcgtgatgcc gttgggatgc ataaattgaa tgttaagctg aaggattttc actttggtgg aactcggtac ttgaaggata tgccacctga ggagcaggag aggcgcttgg aaaggaagaa gcgtattgag gaggcacgcc gtcgaggtaa cgcagcgcgt gaaacgcaca cggataattg gttgctaaac gacacgtttg actaccagca agaggacaga aaggtggaat ttgaggaaaa cggctggatg actccagagg cgtatgtgaa gcatctgggt ctcaaaccat gtggcgatgt cacggcggct gcaagtccca ccgaacctat tcatgttctt gtcgtcttgc caagaccggc ggcggcggcg ccaaaagatg tcaagcggga tgttcctttg gctacatctg aagaatccat ccgtctttta atgaatccgg ccgcgcagcg tgtgttgaca ggaaaggccc ggaatgtcac tcttccttct cccttgtcgt ttggtggtgt gaaaatcact gttctttttg atggggatga tattgactgt attcatcccg attttgtggt tctgcaacat cttcttgccg ccattgaaga ggatgaagcg gcgaaggcac gtgtgaagta ttgggctcac gtggcacgct attgcgtgtt tgtcgtggag acggatgtca gggaccgacg tcattttctc ctccgtgagg aagtacgggt cgcctacgag gatgttgccg aggactgctt tagatccctt catgcggcgg cgtactcaac aaaatgcaat cgtttgagaa cgacgccttc tcttatcgcc ctcagtaata gcaaaaatat tgggctccgc tttaagttca ggggttcacc cttaaataca atagccctca tcgtggttgg ggagcggtta gattga

Details for TrcrB.19530.a.B1.GE41522

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	95kDa
OBSERVED MW:	93kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK QKRGKQDVKM VESAPPQLLP KKGRLEISEL APQQRTIRTA EEIEMAYNEA VRKHPFYDNA DHTIDFHDAT VFRDARGVVG GVLLPGALPA FAATMAADVL RPAAVRTSLR SNMFGGFAPL SGIAGYFDYR GSPVELKCRK TSFTYENVHS WPNVFPMIDY VSAIYKAVFP ERWAAQDAAV PDIVRIHGSP FSTLTVNQQF RTASHTDAGD FDMGYGLLAV LEGKFEGLSL ALDDFGVCFR MQPRDVLIFN THFFHSNTEP ELNHPKDDWS RLTCVCYYRA ALGEPACVAE YERRLARAKE IGASPPPAVD AILQKDNGNN FNKPAPTFTY SLTPFGGAAS ICSLHCCTAK LLRLHELLLE NPTLEVILFG ESLRTDDGLP RREKEQLISV HLPVVVKMSP SGGFSELGGA LKAAEEKQYF FEEKYLADEL GPDLMSMWTQ SRAHWLRLVK EDWERLCRRD PERTKFTWNN SSAMNAAFFD LCEVAKQMMI GLLNKETPSS AENHSFWILF AAHLNYACTT ENGMPRDAVG MHKLNVKLKD FHFGGTRYLK DMPPEEQERR LERKKRIEEA RRRGNAARET HTDNWLLNDT FDYQQEDRKV EFEENGWMTP EAYVKHLGLK PCGDVTAAAS PTEPIHVLVV LPRPAAAAPK DVKRDVPLAT SEESIRLLMN PAAQRVLTGK ARNVTLPSPL SFGGVKITVL FDGDDIDCIH PDFVVLQHLL AAIEEDEAAK ARVKYWAHVA RYCVFVVETD VRDRRHFLLR EEVRVAYEDV AEDCFRSLHA AAYSTKCNRL RTTPSLIALS NSKNIGLRFK FRGSPLNTIA LIVVGERLD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac aaaagagagg aaagcaagac gtgaaaatgg ttgaatcagc tcctccacaa ctgctcccaa aaaagggtcg gcttgagatt tctgaacttg ctccacaaca acggacaata cgtactgcgg aagagattga aatggcatac aatgaggccg ttagaaaaca tcctttttac gataacgccg atcacacgat tgattttcat gacgccactg tattccgtga cgccagaggg gtagtcgggg gcgtcttgtt accaggggcg ctgcctgcat ttgctgccac catggcagcc gatgtgttgc gacctgcagc tgtaaggaca tctttacgat cgaatatgtt tggtgggttc gcacctctta gtggtatcgc cggttacttt gactatcgcg gatctccagt ggaactcaaa tgccgcaaga catcctttac atatgaaaac gttcattcct ggccaaatgt gtttccgatg attgattacg tcagtgccat ttacaaagct gttttcccgg aacggtgggc cgcgcaggat gcagccgtgc cggatattgt ccgcattcac ggatccccct tttccacctt gacggtgaat caacagtttc ggaccgcctc acacacggat gcgggtgatt ttgatatggg atacggtctt ctggcggttc tggagggcaa atttgaaggt ctctccctcg cgttggatga ctttggtgtc tgcttccgca tgcagccaag ggacgtcttg atttttaaca cgcacttctt tcattcgaat acagagccgg agttgaatca tccaaaggac gactggagtc gactaacctg tgtttgctat tatagggcgg cgttggggga accggcgtgt gttgcagaat atgaacgacg tttggcgcgg gctaaagaaa taggtgcctc cccccctcct gctgtagatg ccattttaca aaaggacaat ggcaacaatt ttaacaaacc cgcaccgacg ttcacttatt cactcacacc gtttggagga gcggcgagca tttgctctct gcattgctgc accgccaaac tcctgcggct acacgagcta ctgttagaaa acccaacgtt ggaggttatt ttatttggag aatcgctacg aacggacgat ggcctgccca gacgtgaaaa ggagcaactt atttctgtac accttcctgt ggttgtaaag atgtctccct ccggtgggtt tagtgaattg ggaggggcgc taaaggctgc cgaggagaag cagtacttct ttgaggaaaa atacttggcg gatgaactcg gtccagacct catgtccatg tggacgcaga gtcgtgctca ctggctgagg ctcgtcaagg aggattggga gcgtctctgt cgaagggatc cagaacggac aaaatttacc tggaacaatt cgtctgcgat gaatgcggcc ttttttgatc tctgtgaggt ggcaaagcag atgatgattg gattgctaaa caaggaaaca ccatcatctg ctgagaacca ctccttttgg attctttttg cggcacattt aaattacgca tgcaccactg aaaatggcat gccgcgtgat gccgttggga tgcataaatt gaatgttaag ctgaaggatt ttcactttgg tggaactcgg tacttgaagg atatgccacc tgaggagcag gagaggcgct tggaaaggaa gaagcgtatt gaggaggcac gccgtcgagg taacgcagcg cgtgaaacgc acacggataa ttggttgcta aacgacacgt ttgactacca gcaagaggac agaaaggtgg aatttgagga aaacggctgg atgactccag aggcgtatgt gaagcatctg ggtctcaaac catgtggcga tgtcacggcg gctgcaagtc ccaccgaacc tattcatgtt cttgtcgtct tgccaagacc ggcggcggcg gcgccaaaag atgtcaagcg ggatgttcct ttggctacat ctgaagaatc catccgtctt ttaatgaatc cggccgcgca gcgtgtgttg acaggaaagg cccggaatgt cactcttcct tctcccttgt cgtttggtgg tgtgaaaatc actgttcttt ttgatgggga tgatattgac tgtattcatc ccgattttgt ggttctgcaa catcttcttg ccgccattga agaggatgaa gcggcgaagg cacgtgtgaa gtattgggct cacgtggcac gctattgcgt gtttgtcgtg gagacggatg tcagggaccg acgtcatttt ctcctccgtg aggaagtacg ggtcgcctac gaggatgttg ccgaggactg ctttagatcc cttcatgcgg cggcgtactc aacaaaatgc aatcgtttga gaacgacgcc ttctcttatc gccctcagta atagcaaaaa tattgggctc cgctttaagt tcaggggttc acccttaaat acaatagccc tcatcgtggt tggggagcgg ttagattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for TrcrB.19530.a.B2.GE41523

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT AEEIEMAYNE AVRKHPFYDN ADHTIDFHDA TVFRDARGVV GGVLLPGALP AFAATMAADV LRPAAVRTSL RSNMFGGFAP LSGIAGYFDY RGSPVELKCR KTSFTYENVH SWPNVFPMID YVSAIYKAVF PERWAAQDAA VPDIVRIHGS PFSTLTVNQQ FRTASHTDAG DFDMGYGLLA VLEGKFEGLS LALDDFGVCF RMQPRDVLIF NTHFFHSNTE PELNHPKDDW SRLTCVCYYR AALGEPACVA EYERRLARAK EIG

Validated NT Sequence

atggctcacc accaccacca ccatatgact gcggaagaga ttgaaatggc atacaatgag gccgttagaa aacatccttt ttacgataac gccgatcaca cgattgattt tcatgacgcc actgtattcc gtgacgccag aggggtagtc gggggcgtct tgttaccagg ggcgctgcct gcatttgctg ccaccatggc agccgatgtg ttgcgacctg cagctgtaag gacatcttta cgatcgaata tgtttggtgg gttcgcacct cttagtggta tcgccggtta ctttgactat cgcggatctc cagtggaact caaatgccgc aagacatcct ttacatatga aaacgttcat tcctggccaa atgtgtttcc gatgattgat tacgtcagtg ccatttacaa agctgttttc ccggaacggt gggccgcgca ggatgcagcc gtgccggata ttgtccgcat tcacggatcc cccttttcca ccttgacggt gaatcaacag tttcggaccg cctcacacac ggatgcgggt gattttgata tgggatacgg tcttctggcg gttctggagg gcaaatttga aggtctctcc ctcgcgttgg atgactttgg tgtctgcttc cgcatgcagc caagggacgt cttgattttt aacacgcact tctttcattc gaatacagag ccggagttga atcatccaaa ggacgactgg agtcgactaa cctgtgtttg ctattatagg gcggcgttgg gggaaccggc gtgtgttgca gaatatgaac gacgtttggc gcgggctaaa gaaataggtt aagtgagtaa gat

Expected Protein Sequence

MAHHHHHHTA EEIEMAYNEA VRKHPFYDNA DHTIDFHDAT VFRDARGVVG GVLLPGALPA FAATMAADVL RPAAVRTSLR SNMFGGFAPL SGIAGYFDYR GSPVELKCRK TSFTYENVHS WPNVFPMIDY VSAIYKAVFP ERWAAQDAAV PDIVRIHGSP FSTLTVNQQF RTASHTDAGD FDMGYGLLAV LEGKFEGLSL ALDDFGVCFR MQPRDVLIFN THFFHSNTEP ELNHPKDDWS RLTCVCYYRA ALGEPACVAE YERRLARAKE IG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catactgcgg aagagattga aatggcatac aatgaggccg ttagaaaaca tcctttttac gataacgccg atcacacgat tgattttcat gacgccactg tattccgtga cgccagaggg gtagtcgggg gcgtcttgtt accaggggcg ctgcctgcat ttgctgccac catggcagcc gatgtgttgc gacctgcagc tgtaaggaca tctttacgat cgaatatgtt tggtgggttc gcacctctta gtggtatcgc cggttacttt gactatcgcg gatctccagt ggaactcaaa tgccgcaaga catcctttac atatgaaaac gttcattcct ggccaaatgt gtttccgatg attgattacg tcagtgccat ttacaaagct gttttcccgg aacggtgggc cgcgcaggat gcagccgtgc cggatattgt ccgcattcac ggatccccct tttccacctt gacggtgaat caacagtttc ggaccgcctc acacacggat gcgggtgatt ttgatatggg atacggtctt ctggcggttc tggagggcaa atttgaaggt ctctccctcg cgttggatga ctttggtgtc tgcttccgca tgcagccaag ggacgtcttg atttttaaca cgcacttctt tcattcgaat acagagccgg agttgaatca tccaaaggac gactggagtc gactaacctg tgtttgctat tatagggcgg cgttggggga accggcgtgt gttgcagaat atgaacgacg tttggcgcgg gctaaagaaa taggttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat