TrcrB.19529.b

J2IP1

CENTER ID: TrcrB.19529.b
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.19529.b.B2.GE41521 ordered domain 14 265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.510943.198
UniProt: Q4DRS3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFENRTISRK NAVPLVEFMR LLRNQSSNEG RTIFPRRYNG RLAAPSMPMV QKNIISQELK VTEEVETLKY EPQRKNWVRE KRRRSPSIDK LLQMKFLRSP FACEILTKSS KGMYSSTYVD IELKSLGLHP LVQEVPTTRK ETKLLIGVSC CYLRLGTALV VSGCIVEVNI TDSTAQIVPD KGLCLDVEWV SLKKLYCIPD NLRDVRRIVS EKVYAIIQGE MEDPMRVVKT DLPGGEQWEE IPLSQSQDGG VAEETEDTNK HVYNE
NT Sequence
atgtttgaga atcgaacgat ttccaggaaa aatgctgttc ctttggttga atttatgagg ctgctccgca atcaaagcag caacgaggga aggacgatct ttcctagacg ctataacggg cgactggcgg cgccttcgat gccgatggta caaaaaaata ttatttcaca agagttgaaa gtcacagagg aagtggaaac gttaaaatat gaaccacaac gtaaaaattg ggtcagggaa aaacgaagac gaagtccatc cattgataaa ttgctgcaaa tgaagtttct gcgctcgccc tttgcgtgtg agattttgac caaatcttca aagggtatgt atagttctac ctatgtcgat attgaactaa aatcgcttgg tctgcaccct ctggtgcagg aagttcccac cactcgtaaa gagacaaagt tattgatagg agtttcttgt tgttatctga gattggggac cgcgcttgtt gtgagcggct gcatcgttga agttaacatc acagattcta cggcacaaat agttccagac aagggattat gtctcgacgt ggagtgggtg agtttaaaaa agctctactg cattcctgac aatcttaggg acgtaaggcg aatcgtctcg gaaaaagttt acgcaattat tcaaggggaa atggaagatc caatgcgtgt ggtaaaaacc gatttacccg gtggggagca atgggaagaa attcctttgt cccagtctca agacggtggc gtggcagaag aaacggagga tacgaataaa catgtttaca atgaataa
Details for TrcrB.19529.b.B2.GE41521
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP LVEFMRLLRN QSSNEGRTIF PRRYNGRLAA PSMPMVQKNI ISQELKVTEE VETLKYEPQR KNWVREKRRR SPSIDKLLQM KFLRSPFACE ILTKSSKGMY SSTYVDIELK SLGLHPLVQE VPTTRKETKL LIGVSCCYLR LGTALVVSGC IVEVNITDST AQIVPDKGLC LDVEWVSLKK LYCIPDNLRD VRRIVSEKVY AIIQGEMEDP MRVVKTDLPG GEQWEEIPLS QSQDGGVAEE TEDTNKHVYN E
Validated NT Sequence
atggctcacc accaccacca ccatatgcct ttggttgaat ttatgaggct gctccgcaat caaagcagca acgagggaag gacgatcttt cctagacgct ataacgggcg actggcggcg ccttcgatgc cgatggtaca aaaaaatatt atttcacaag agttgaaagt cacagaggaa gtggaaacgt taaaatatga accacaacgt aaaaattggg tcagggaaaa acgaagacga agtccatcca ttgataaatt gctgcaaatg aagtttctgc gctcgccctt tgcgtgtgag attttgacca aatcttcaaa gggtatgtat agttctacct atgtcgatat tgaactaaaa tcgcttggtc tgcaccctct ggtgcaggaa gttcccacca ctcgtaaaga gacaaagtta ttgataggag tttcttgttg ttatctgaga ttggggaccg cgcttgttgt gagcggctgc atcgttgaag ttaacatcac agattctacg gcacaaatag ttccagacaa gggattatgt ctcgacgtgg agtgggtgag tttaaaaaag ctctactgca ttcctgacaa tcttagggac gtaaggcgaa tcgtctcgga aaaagtttac gcaattattc aaggggaaat ggaagatcca atgcgtgtgg taaaaaccga tttacccggt ggggagcaat gggaagaaat tcctttgtcc cagtctcaag acggtggcgt ggcagaagaa acggaggata cgaataaaca tgtttacaat gaataagtga gtaagat
Expected Protein Sequence
MAHHHHHHPL VEFMRLLRNQ SSNEGRTIFP RRYNGRLAAP SMPMVQKNII SQELKVTEEV ETLKYEPQRK NWVREKRRRS PSIDKLLQMK FLRSPFACEI LTKSSKGMYS STYVDIELKS LGLHPLVQEV PTTRKETKLL IGVSCCYLRL GTALVVSGCI VEVNITDSTA QIVPDKGLCL DVEWVSLKKL YCIPDNLRDV RRIVSEKVYA IIQGEMEDPM RVVKTDLPGG EQWEEIPLSQ SQDGGVAEET EDTNKHVYNE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcctttgg ttgaatttat gaggctgctc cgcaatcaaa gcagcaacga gggaaggacg atctttccta gacgctataa cgggcgactg gcggcgcctt cgatgccgat ggtacaaaaa aatattattt cacaagagtt gaaagtcaca gaggaagtgg aaacgttaaa atatgaacca caacgtaaaa attgggtcag ggaaaaacga agacgaagtc catccattga taaattgctg caaatgaagt ttctgcgctc gccctttgcg tgtgagattt tgaccaaatc ttcaaagggt atgtatagtt ctacctatgt cgatattgaa ctaaaatcgc ttggtctgca ccctctggtg caggaagttc ccaccactcg taaagagaca aagttattga taggagtttc ttgttgttat ctgagattgg ggaccgcgct tgttgtgagc ggctgcatcg ttgaagttaa catcacagat tctacggcac aaatagttcc agacaaggga ttatgtctcg acgtggagtg ggtgagttta aaaaagctct actgcattcc tgacaatctt agggacgtaa ggcgaatcgt ctcggaaaaa gtttacgcaa ttattcaagg ggaaatggaa gatccaatgc gtgtggtaaa aaccgattta cccggtgggg agcaatggga agaaattcct ttgtcccagt ctcaagacgg tggcgtggca gaagaaacgg aggatacgaa taaacatgtt tacaatgaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat