TrcrB.19528.b

J-binding protein 2 (JBP2)

CENTER ID: TrcrB.19528.b
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.19528.b.B4.GE41517 helicase domain 515 1086
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_79437
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.508859.74
UniProt: Q4DCH3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVPSHVSVA TLQSVLQTVC STGEPAIIAP SSFLGELDTV VDEVKRHGMK LASIPHGGIT ILPPVPISGT ELFDFCAEYC KAQTHEERLA VRHKINNHNF LMQDPLLRMP CRQLYNPADY VLRIVHLCSE LVSASEEEYH GAYGVAPLLH INPVQDVCGK LRSMFQSGSL YVKPWILEEE EERREMDESN RGVLFNSDSF GNGRGGSSIS SSERSVDEND VADEDLTGEE VVDNATDTVV EYLSEKDFDV VTESGIFYDD SGERVHAIYL RGGIKKELCQ RAAIAIEEAA TTKNLRKAVN GGKTNPETGI VGYYDYLNNP TQRKCRETEF TRKNWSSVVD SCEPFLVALN KLYSECAPTH YKLQRIAIPH HYQLFNTVFS TMTVNRNFRT AVHTDRGDFR SGLAALCVID GVFEGCHLAI KKLGKAFRLE TGDVLFFDTS LEHGNTEVHN FDYCWKRVSV VCYLRNGLMS QICEMERRRW LQKQMLKQRL LDRSRQSVIN LNATDPNLPP IYLPGRLLEV LSPVQQAALG FVVDRLSKGN GCVIALTMGL GKTLLSLALC YSHMYDQNPR DVLILAPKIV LTHWTGEKQK WEKYGLVFSH FVVSDGTDSV SFEIALKRYK QQLNGELPRT SHVFVINPEY IRTVLKKLTG FRPSLIIVDE GHRVSSKGSK LKDWLEGLRC TARVILSGTP VQNNAEELYR LIGWINSDVH SVLPPRVFTD LAGTINRYIN GDDSALAAAV SAQRYIQEWM CSYVFSVMKT DLPPLNDYII ICGFSSIQRK MLEDHFGMEG IDGLTSIKAS EHRPYHLSTH PLCFLGFISG VYKSLNGNHK LTPEAEEELE SQEYASQLYS LTEDDIGLID ECLSLVNSGF LTEFVGLSGK MTVLISILHS IREKKEKAII FSQYVGSQDF ISRTLTSFDI VSSTIRGRDC HERRRRTIEK FREDEKITCL LLSTQIGAYG LDFTAANHVI LWDSWWNPQV ESQAIARAYR RNQTRAVIVY RLASEFEDTI VLKTQIRKLA LFRCIMNEEA SRAVPPEELL DCVDTEEDEG RRFLWRSLKK SYLEGGAPAV SKVFRHGDTV RSESWS
NT Sequence
atgcctgtcc cttcccatgt aagcgttgcg accctgcagt cggtattaca aaccgtttgc tccacagggg agccagccat aattgccccg tcctcgtttt tgggagagct ggacaccgtc gtcgatgagg taaaaagaca tggaatgaaa ctggcaagca ttcctcatgg tggaatcact attcttcccc ctgttccaat cagtggtacc gaactcttcg acttttgcgc agagtattgt aaagctcaaa cgcacgaaga gaggttggcc gtgcgtcata aaataaataa ccataatttc ttaatgcagg accctcttct gcggatgccc tgccgacaac tgtataaccc ggccgactat gtgctgcgta tagttcacct ctgctcagag cttgtttctg cttcagagga agagtaccac ggggcctacg gcgtcgcacc acttcttcat attaaccctg ttcaagatgt ttgcggcaag ctgcgcagca tgtttcaatc tggcagcctt tacgtcaagc cttggattct cgaggaggag gaggaacgac gagaaatgga cgaaagcaac agaggcgtgc ttttcaattc agattctttt ggaaatggcc ggggcggcag cagcatcagc agcagtgaac ggagcgtcga tgagaacgac gtggcagacg aggatctaac cggagaggag gttgttgaca acgcgacgga tacagtggtg gagtacctct ctgaaaagga ctttgacgtg gtaacggaga gcggtatatt ctatgatgat tctggggaac gggtgcatgc catttatctc cgaggtggaa ttaagaagga actctgtcag cgggccgcta ttgccattga agaagctgcg acgaccaaaa atctgcgcaa agccgtcaat ggagggaaaa cgaacccgga aacgggtatt gtgggctact atgactactt gaataatccc acacagcgca agtgccgtga gacagagttt acacgaaaaa attggagttc tgtagtcgac tcctgcgagc catttcttgt ggcactaaat aaactttaca gcgagtgtgc cccgacgcat tacaaacttc aacgcattgc tataccgcac cattatcaat tgtttaatac ggtttttagc acaatgacgg ttaatcgaaa ttttcgaact gccgttcaca cggatcgggg tgacttccgc agtggactgg ccgctctctg cgtgatagac ggggtctttg aagggtgcca tttggcgatt aaaaagttgg ggaaggcatt tcgtcttgaa acaggggatg ttttgttctt tgatacttcc ctggaacacg ggaacaccga ggtccacaac tttgattatt gctggaagcg ggtgagcgtt gtctgctacc tgcgaaatgg cctcatgtct caaatatgcg agatggaacg acgccgatgg cttcagaagc agatgttgaa gcaacgtctt ctggatagga gtcgccagag tgttataaac ctaaatgcca cggacccaaa tcttcctccc atataccttc ctggaagatt gctggaggtt ctctcccccg ttcaacaagc cgctcttgga tttgttgtgg atcgacttag caaaggcaac gggtgtgtaa ttgcgctgac gatgggattg ggaaaaacgt tgctttccct ggcactgtgc tattcgcata tgtatgacca aaatccacgg gatgtactta ttttggcacc gaagattgtt cttactcatt ggacaggaga aaagcaaaag tgggaaaagt acggccttgt tttttcacat tttgtcgtct ccgatgggac ggactccgtt tcctttgaaa tcgcactcaa gcgatataaa caacagctca atggggagtt gcccagaacc agtcatgtgt ttgtcatcaa ccccgagtac atccggacgg tcctgaaaaa actcaccgga tttcgtccaa gtcttattat tgtcgatgag ggtcaccgtg tgtcctccaa gggaagcaaa ttgaaggact ggttggaggg actgcggtgc acggcgcgtg tgatactctc tggaacgcct gtgcagaaca atgcggagga attataccga ctcatcggct ggataaacag cgacgttcat tctgttctac ccccacgtgt gtttacggat cttgcgggga ctatcaatcg ttacattaat ggagatgatt cggcacttgc cgcagccgtg tctgcgcaac gttatattca agagtggatg tgttcctatg tttttagcgt catgaagaca gaccttccac cacttaatga ctatattatt atttgtggat tttccagcat tcaaaggaaa atgcttgagg atcattttgg gatggaaggg atcgatgggc tgacaagcat aaaggcatct gagcaccgtc catatcacct ctccacacat ccgctttgtt ttttgggatt tatatccgga gtctataaat cgcttaatgg aaatcataag ttgacgcctg aggcggagga agagcttgag tcgcaggagt atgcatcgca gctatattca ctgaccgaag atgacattgg actgattgat gagtgtttat cgctggtgaa ctctggtttc ctaacggagt ttgtgggcct aagtgggaag atgactgttc tcatttcaat attgcattca attcgtgaaa aaaaggagaa ggccatcatc ttctctcaat acgtcggatc acaagacttt atctcgcgaa cattgacttc atttgacatc gtctcctcca ccatccgcgg aagggactgt catgaacgtc gaagacgaac aatagagaaa tttagagagg atgagaaaat tacttgcctt ctcttatcaa ctcaaattgg cgcgtatggc cttgacttca ctgccgcaaa ccacgttatc ctgtgggaca gttggtggaa ccctcaagtg gagtcgcagg ccatcgcacg agcatacaga aggaatcaaa cacgagcagt cattgtttac cgtcttgcct cggagttcga ggatacaatt gtcctcaaaa cgcagataag gaaactggca ctattcagat gtattatgaa tgaggaagca tcacgtgcgg tacctcctga agagctgctg gattgcgttg acaccgaaga agatgaagga aggcgctttt tatggcgttc ccttaagaag agttacctgg agggtggtgc accggcggta tccaaggtat tccgtcatgg cgacacggtg agaagtgaat catggtctta a
Details for TrcrB.19528.b.B4.GE41517
HARVESTED ON: data unavailable
SEQUENCED ON: 5/3/2017
EXPECTED MW: 66kDa
OBSERVED MW: 16kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG RLLEVLSPVQ QAALGFVVDR LSKGNGCVIA LTMGLGKTLL SLALCYSHMY DQNPRDVLIL APKIVLTHWT GEKQKWEKYG LVFSHFVVSD GTDSVSFEIA LKRYKQQLNG ELPRTSHVFV INPEYIRTVL KKLTGFRPSL IIVDEGHRVS SKGSKLKDWL EGLRCTARVI LSGTPVQNNA EELYRLIGWI NNDVHSVLPP RVFTDLAGTI NRYINGDDSA LAAAVSAQRY IXEWMCSYVF SVMKXDLPPL NDYIIICGFS SXXRKMLXXX XXMEGIDGLT SIKASEHRPY HLSTHPLXXX XXXSGVYKSL NGNHKLTPEA EEELESQEYA SQLYSLTEDD IGLIDECLSL VNSGFLTEFV GLSGKMTVLI SILHSIREKK EKAIIFSQYV GSQDFISRTL TSFDIVSSTI RGRDCHERRR RTIEKFREDE KITCLLLSTQ IGAYGLDFTA ANHVILWDSW WNPQVESQAI ARAYRRNQTR AVIVYRLASE FEDTIVLKTQ IRKLALFRCI MNEEASRAVP PEELLDCVDT EEDEGRRFLW RSLKKSYLEG GAPAVSKVFR HGD
Validated NT Sequence
atggctcacc accaccacca ccatatggga agattgctgg aggttctctc ccccgttcaa caagccgctc ttggatttgt tgtggatcga cttagcaaag gcaacgggtg tgtaattgcg ctgacgatgg gattgggaaa aacgttgctt tccctggcac tgtgctattc gcatatgtat gaccaaaatc cacgggatgt acttattttg gcaccgaaga ttgttcttac tcattggaca ggagaaaagc aaaagtggga aaagtacggc cttgtttttt cacattttgt cgtctccgat gggacggact ccgtttcctt tgaaatcgca ctcaagcgat ataaacaaca gctcaatggg gagttgccca gaaccagtca tgtgtttgtt atcaaccccg agtacatccg gacggtcctg aaaaaactca ccggatttcg tccaagtctt attattgtcg atgagggaca ccgtgtgtcc tccaagggaa gcaaattgaa ggactggttg gaggggctgc ggtgcacggc gcgtgtgata ctctctggaa cgcctgtgca gaacaatgcg gaggaattat accgactcat cggctggata aacaacgacg ttcattctgt tctaccccca cgtgtgttta cggatcttgc ggggactatc aatcgttaca ttaatggaga tgattcggca cttgccgcag ccgtgtctgc gcaacgttat attcnngagt ggatgtgttc ctatgttttt agcgtcatga agncagacct tccaccactt aatgactata ttattatttg tggattttcc agcatnnnaa ggaaaatgct tgannnnnnn ttnnnnatgg aagggatcga tggactgaca agcataaagg catctgagca ccgtccatat cacctctcca cacatccgct nnnnnnnnnn nnnntnnnnt ccggagtcta taaatcgctt aatggaaatc ataagttgac gcctgaggcg gaggaagagc ttgagtcgca ggagtatgca tcgcagctat attcactgac cgaagatgac attggactga ttgatgagtg tttatcgctg gtgaactctg gtttcctaac ggagtttgtg ggcctaagtg ggaagatgac tgttctcatt tcaatattgc attcaattcg tgaaaaaaag gagaaggcca tcatcttctc tcaatacgtc ggatcacaag actttatctc gcgaacattg acttcatttg acatcgtctc ctccaccatc cgcggaaggg actgtcatga acgtcgaaga cgaacaatag agaaatttag agaggatgag aaaattactt gccttctctt atcaactcaa attggcgcgt atggccttga cttcactgcc gcaaaccacg ttatcctgtg ggacagttgg tggaaccctc aagtggagtc gcaggccatc gcacgagcat acagaaggaa tcaaacacga gcagtcattg tttaccgtct tgcctcggag ttcgaggata caattgtcct caaaacgcag ataaggaaac tggcactatt cagatgtatt atgaatgagg aagcatcacg tgcggtacct cctgaagagc tgctggattg cgttgacacc gaagaagatg aaggaaggcg ctttttatgg cgttccctta agaagagtta cctggagggt ggtgcaccgg cggtatccaa ggtattccgt catggcgaca cg
Expected Protein Sequence
MAHHHHHHGR LLEVLSPVQQ AALGFVVDRL SKGNGCVIAL TMGLGKTLLS LALCYSHMYD QNPRDVLILA PKIVLTHWTG EKQKWEKYGL VFSHFVVSDG TDSVSFEIAL KRYKQQLNGE LPRTSHVFVI NPEYIRTVLK KLTGFRPSLI IVDEGHRVSS KGSKLKDWLE GLRCTARVIL SGTPVQNNAE ELYRLIGWIN SDVHSVLPPR VFTDLAGTIN RYINGDDSAL AAAVSAQRYI QEWMCSYVFS VMKTDLPPLN DYIIICGFSS IQRKMLEDHF GMEGIDGLTS IKASEHRPYH LSTHPLCFLG FISGVYKSLN GNHKLTPEAE EELESQEYAS QLYSLTEDDI GLIDECLSLV NSGFLTEFVG LSGKMTVLIS ILHSIREKKE KAIIFSQYVG SQDFISRTLT SFDIVSSTIR GRDCHERRRR TIEKFREDEK ITCLLLSTQI GAYGLDFTAA NHVILWDSWW NPQVESQAIA RAYRRNQTRA VIVYRLASEF EDTIVLKTQI RKLALFRCIM NEEASRAVPP EELLDCVDTE EDEGRRFLWR SLKKSYLEGG APAVSKVFRH GDTVRSESWS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggaagat tgctggaggt tctctccccc gttcaacaag ccgctcttgg atttgttgtg gatcgactta gcaaaggcaa cgggtgtgta attgcgctga cgatgggatt gggaaaaacg ttgctttccc tggcactgtg ctattcgcat atgtatgacc aaaatccacg ggatgtactt attttggcac cgaagattgt tcttactcat tggacaggag aaaagcaaaa gtgggaaaag tacggccttg ttttttcaca ttttgtcgtc tccgatggga cggactccgt ttcctttgaa atcgcactca agcgatataa acaacagctc aatggggagt tgcccagaac cagtcatgtg tttgtcatca accccgagta catccggacg gtcctgaaaa aactcaccgg atttcgtcca agtcttatta ttgtcgatga gggtcaccgt gtgtcctcca agggaagcaa attgaaggac tggttggagg gactgcggtg cacggcgcgt gtgatactct ctggaacgcc tgtgcagaac aatgcggagg aattataccg actcatcggc tggataaaca gcgacgttca ttctgttcta cccccacgtg tgtttacgga tcttgcgggg actatcaatc gttacattaa tggagatgat tcggcacttg ccgcagccgt gtctgcgcaa cgttatattc aagagtggat gtgttcctat gtttttagcg tcatgaagac agaccttcca ccacttaatg actatattat tatttgtgga ttttccagca ttcaaaggaa aatgcttgag gatcattttg ggatggaagg gatcgatggg ctgacaagca taaaggcatc tgagcaccgt ccatatcacc tctccacaca tccgctttgt tttttgggat ttatatccgg agtctataaa tcgcttaatg gaaatcataa gttgacgcct gaggcggagg aagagcttga gtcgcaggag tatgcatcgc agctatattc actgaccgaa gatgacattg gactgattga tgagtgttta tcgctggtga actctggttt cctaacggag tttgtgggcc taagtgggaa gatgactgtt ctcatttcaa tattgcattc aattcgtgaa aaaaaggaga aggccatcat cttctctcaa tacgtcggat cacaagactt tatctcgcga acattgactt catttgacat cgtctcctcc accatccgcg gaagggactg tcatgaacgt cgaagacgaa caatagagaa atttagagag gatgagaaaa ttacttgcct tctcttatca actcaaattg gcgcgtatgg ccttgacttc actgccgcaa accacgttat cctgtgggac agttggtgga accctcaagt ggagtcgcag gccatcgcac gagcatacag aaggaatcaa acacgagcag tcattgttta ccgtcttgcc tcggagttcg aggatacaat tgtcctcaaa acgcagataa ggaaactggc actattcaga tgtattatga atgaggaagc atcacgtgcg gtacctcctg aagagctgct ggattgcgtt gacaccgaag aagatgaagg aaggcgcttt ttatggcgtt cccttaagaa gagttacctg gagggtggtg caccggcggt atccaaggta ttccgtcatg gcgacacggt gagaagtgaa tcatggtctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat