TrcrB.19528.a

J-binding protein 2 (JBP2)

CENTER ID: TrcrB.19528.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.19528.a.B1.GE41512 full length 1 1087
TrcrB.19528.a.B2.GE41513 N-term conserved domain 12 162
TrcrB.19528.a.B3.GE41514 Tet_JBP oxygenase 226 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.19528.a.B3.PS38310 Tet_JBP oxygenase 226 491
Molecular models
METHOD RESULTS
comparative modelling Robetta_79438
External Resources
RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.506605.229
UniProt: Q4DUV6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVPSHVSVT TLQSVLQTVC STGEPAIIAP SSFLGELDTV VDEVKRHGMK LASIPHGGIT ILPPVPISGT ELFDFCAEYC KAQTHEERLA VRHKINNHNF LMQDPLLRMP CRQLYNPADY VLRIVHLCSE LVSASEEEYH GAYGVAPLLH INPVQDVCGK LRSMFQSGSL YVKPWILDEE EERREMDESN RGVLFNSDSF GNGRGGSSIS SSSERSVDEN DVADEDLTGQ EVVDNATDTV VEYLSEKDFD VVTESGIFYD DSGERVHAIY LRGGIKKELC QRAAIAIEEA ATTKNLRKAV NGGKTNPETG IVGYYDYLNN PTQRKCRETE FTRKNWSSVV DSCEPFLVAL NKLYSECAPT HYKLQRIAIP RHYQLFNTVF STMTVNRNFR TAVHTDRGDF RSGLAALCVI DGVFEGCHLA IKKLGKAFRL ETGDVLFFDT SLEHGNTEVH NFDYCWKRVS VVCYLRNGLM SQICEMERRQ WLQKQMLKQR LLDRSRQSVI NLNATDPNLP PIYLPGRLLE VLSPVQQAAL GFVVDRLSKG NGCVIALTMG LGKTLLSLAL CYSHMYDQNP RDVLILAPKI VLTHWTGEKQ KWEKYGLVFS HFVVSDGTDS VSFEIALKRY KQQLNGELPR TSHVFVINPE YIRTVLKKLT GFRPSLIIVD EGHRVSSKGS KLKDWLEGLR CTARVILSGT PVQNNAEELY RLIGWINSDV HSVLPPRVFT DLAGTINRYI NGDDSALAAA VSAQRYIQEW MCSYVFSVMK TDLPPLNDYI IICGFSSIQR KMLEDHFGME GIDGLTSIKA SEHRPYHLST HPLCFLGFIS GVYKSLNGNH KLTPEAEEEL ESQEYASQLY SLTEDDIGLI DECLSLVSSG FLTEFVGLSG KMTVLISILH SIREKKEKAI IFSQYVGSQD FISRTLTSFD IVSSTIRGRD CHERRRRTIE KFREDENITC LLLSTQIGAY GLDFTAANHV ILWDSWWNPQ VESQAIARAY RRNQTRAVIV YRLASEFEDT IVLKTQIRKL ALFRCIMNEE ASRAVPPEEL LDCVDTEEDE GRRFLWRSLK KSYLEGGAPA VSKVFRHGDT VRSESWS
NT Sequence
atgcctgtcc cttcccatgt aagcgttacg accctgcagt cggtattaca aaccgtttgc tccacagggg agccagccat aattgccccg tcctcttttt tgggagagct ggacaccgtc gtcgatgagg taaaaagaca tggaatgaaa ctggcaagca ttcctcatgg tggaatcact attcttcctc ctgttccaat cagtggtacc gaactcttcg acttttgcgc agagtattgt aaagctcaaa cgcacgaaga gaggttggcc gtgcgtcata aaataaataa ccataatttc ttaatgcagg accctcttct gcggatgccc tgtcgacaac tgtataaccc agccgactat gtgctgcgta tagttcacct ctgctcagag cttgtttctg cttcagagga agagtaccac ggggcctacg gcgtcgcacc acttcttcat attaaccctg ttcaagatgt ttgcggcaag ctgcgcagca tgtttcaatc tggcagcctt tacgtcaagc cttggattct cgatgaggag gaggaacgac gagaaatgga cgaaagcaac agaggcgtgc ttttcaattc ggattctttt ggaaatggcc ggggcggcag cagcatcagc agcagcagtg aacggagcgt cgatgagaac gacgtggcag acgaggatct aactggacag gaggttgttg acaacgcgac ggatacagtg gtggagtacc tctccgaaaa ggactttgac gtggtaacgg agagcggcat attctatgat gattctgggg aacgggtgca tgccatttat cttcgaggtg gaattaagaa ggaactctgt cagcgggccg ctattgccat tgaagaagct gcgacgacca aaaatctgcg caaagccgtc aatggaggga aaacgaaccc ggaaacgggt attgtgggct actatgacta cttgaataat cccacacagc gcaagtgccg tgagacagag tttacacgaa aaaattggag ttctgtagtc gactcctgcg agccatttct tgtggcacta aataaacttt acagtgagtg tgccccgacg cattacaaac ttcaacgcat tgctatacca cgccattatc aattgtttaa tacggttttt agtacaatga cggttaatcg aaattttcga actgctgttc acacggatcg gggtgacttc cgcagtggat tggccgctct ctgtgtgata gacggggtct ttgaagggtg ccatttggcg attaaaaagt tggggaaggc atttcgtctt gaaacggggg atgttttgtt ctttgataca tccctggaac acgggaacac cgaggtccac aactttgatt attgctggaa gcgggtgagc gttgtctgct acctgcgaaa tggtctcatg tctcaaatat gcgagatgga acgacgccag tggcttcaaa agcagatgtt gaagcaacgt cttctggata ggagtcgcca gagtgttata aacctaaatg ccacggaccc aaaccttcct cccatatacc ttcctggaag attgctggag gttctctccc ccgttcaaca agccgctctt ggatttgttg tggatcgact tagcaaaggc aacgggtgtg taattgcgct gacgatggga ttgggaaaaa cgttgctttc cctggcactg tgctattcgc atatgtatga ccaaaatcca cgggatgtac ttattttggc accgaagatt gttcttactc attggacagg agaaaagcaa aagtgggaaa agtacggcct tgttttttca cattttgtcg tctccgatgg gacggactct gtttcctttg aaatcgcact caagcgatat aaacaacagc tcaatgggga gttgcccaga accagtcatg tgtttgtcat caaccccgag tacatccgga cggtcctgaa aaaactcacc ggatttcgtc caagtcttat tattgtcgat gagggtcacc gtgtgtcctc caagggaagc aaattgaagg actggttgga gggactgcgg tgcacggcgc gtgtgatact ctctggaacg cctgtgcaga acaatgcgga ggaattatac cgactcatcg gctggataaa cagcgacgtt cattctgttc tacccccacg tgtgtttacg gatcttgcgg ggactatcaa tcgttacatt aatggagatg attcggcact tgccgcagcc gtgtctgcgc aacgttatat tcaagagtgg atgtgttcct atgtttttag cgtcatgaag acagaccttc caccacttaa tgactatatt attatttgtg gattttccag cattcaaagg aaaatgcttg aggatcattt tgggatggaa gggatcgatg ggctgacaag cataaaggca tctgagcacc gtccatatca cctctccaca catccgcttt gttttttggg atttatatcc ggagtctata aatcgcttaa tggaaatcat aagttgacgc ctgaggcgga ggaagagctt gagtcgcagg agtatgcatc gcagctatat tcactgaccg aagatgacat tggactgatt gatgagtgtt tatcgctggt gagctctggt ttcctaacgg agtttgtggg cttaagtggg aagatgactg ttctcatttc gatattgcat tcaattcgtg aaaaaaagga gaaggccatc atcttctctc aatacgtcgg atcacaagac tttatctcgc gaacattgac ttcatttgac atcgtctcct ccaccatccg cggaagggac tgtcatgaac gtcgaagacg aacaatagag aaatttagag aggatgagaa cattacttgc cttctcttat caactcaaat tggcgcgtat ggccttgact tcactgccgc aaaccacgtt atcctgtggg acagttggtg gaaccctcaa gtggagtcgc aggccatcgc acgagcatac agaagaaatc aaacacgagc agtcattgtt taccgtcttg cctcggagtt cgaggataca attgtcctca aaacgcagat aaggaaactg gcactattca gatgtattat gaatgaggaa gcatcacgtg cggtacctcc tgaagagctg ctggattgcg ttgacaccga agaagatgag ggaagacgct ttttatggcg ttcccttaag aagagttacc tggagggtgg tgcaccggcg gtatccaagg tattccgtca tggcgacacg gtgagaagtg aatcatggtc ttaa
Details for TrcrB.19528.a.B1.GE41512
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 124kDa
OBSERVED MW: 124kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMP VPSHVSVTTL QSVLQTVCST GEPAIIAPSS FLGELDTVVD EVKRHGMKLA SIPHGGITIL PPVPISGTEL FDFCAEYCKA QTHEERLAVR HKINNHNFLM QDPLLRMPCR QLYNPADYVL RIVHLCSELV SASEEEYHGA YGVAPLLHIN PVQDVCGKLR SMFQSGSLYV KPWILDEEEE RREMDESNRG VLFNSDSFGN GRGGSSISSS SERSVDENDV ADEDLTGQEV VDNATDTVVE YLSEKDFDVV TESGIFYDDS GERVHAIYLR GGIKKELCQR AAIAIEEAAT TKNLRKAVNG GKTNPETGIV GYYDYLNNPT QRKCRETEFT RKNWSSVVDS CEPFLVALNK LYSECAPTHY KLQRIAIPRH YQLFNTVFST MTVNRNFRTA VHTDRGDFRS GLAALCVIDG VFEGCHLAIK KLGKAFRLET GDVLFFDTSL EHGNTEVHNF DYCWKRVSVV CYLRNGLMSQ ICEMERRQWL QKQMLKQRLL DRSRQSVINL NATDPNLPPI YLPGRLLEVL SPVQQAALGF VVDRLSKGNG CVIALTMGLG KTLLSLALCY SHMYDQNPRD VLILAPKIVL THWTGEKQKW EKYGLVFSHF VVSDGTDSVS FEIALKRYKQ QLNGELPRTS HVFVINPEYI RTVLKKLTGF RPSLIIVDEG HRVSSKGSKL KDWLEGLRCT ARVILSGTPV QNNAEELYRL IGWINSDVHS VLPPRVFTDL AGTINRYING DDSALAAAVS AQRYIQEWMC SYVFSVMKTD LPPLNDYIII CGFSSIQRKM LEDHFGMEGI DGLTSIKASE HRPYHLSTHP LCFLGFISGV YKSLNGNHKL TPEAEEELES QEYASQLYSL TEDDIGLIDE CLSLVSSGFL TEFVGLSGKM TVLISILHSI REKKEKAIIF SQYVGSQDFI SRTLTSFDIV SSTIRGRDCH ERRRRTIEKF REDENITCLL LSTQIGAYGL DFTAANHVIL WDSWWNPQVE SQAIARAYRR NQTRAVIVYR LASEFEDTIV LKTQIRKLAL FRCIMNEEAS RAVPPEELLD CVDTEEDEGR RFLWRSLKKS YLEGGAPAVS KVFRHGDTVR SESWS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctg tcccttccca tgtaagcgtt acgaccctgc agtcggtatt acaaaccgtt tgctccacag gggagccagc cataattgcc ccgtcctctt ttttgggaga gctggacacc gtcgtcgatg aggtaaaaag acatggaatg aaactggcaa gcattcctca tggtggaatc actattcttc ctcctgttcc aatcagtggt accgaactct tcgacttttg cgcagagtat tgtaaagctc aaacgcacga agagaggttg gccgtgcgtc ataaaataaa taaccataat ttcttaatgc aggaccctct tctgcggatg ccctgtcgac aactgtataa cccagccgac tatgtgctgc gtatagttca cctctgctca gagcttgttt ctgcttcaga ggaagagtac cacggggcct acggcgtcgc accacttctt catattaacc ctgttcaaga tgtttgcggc aagctgcgca gcatgtttca atctggcagc ctttacgtca agccttggat tctcgatgag gaggaggaac gacgagaaat ggacgaaagc aacagaggcg tgcttttcaa ttcggattct tttggaaatg gccggggcgg cagcagcatc agcagcagca gtgaacggag cgtcgatgag aacgacgtgg cagacgagga tctaactgga caggaggttg ttgacaacgc gacggataca gtggtggagt acctctccga aaaggacttt gacgtggtaa cggagagcgg catattctat gatgattctg gggaacgggt gcatgccatt tatcttcgag gtggaattaa gaaggaactc tgtcagcggg ccgctattgc cattgaagaa gctgcgacga ccaaaaatct gcgcaaagcc gtcaatggag ggaaaacgaa cccggaaacg ggtattgtgg gctactatga ctacttgaat aatcccacac agcgcaagtg ccgtgagaca gagtttacac gaaaaaattg gagttctgta gtcgactcct gcgagccatt tcttgtggca ctaaataaac tttacagtga gtgtgccccg acgcattaca aacttcaacg cattgctata ccacgccatt atcaattgtt taatacggtt tttagtacaa tgacggttaa tcgaaatttt cgaactgctg ttcacacgga tcggggtgac ttccgcagtg gattggccgc tctctgtgtg atagacgggg tctttgaagg gtgccatttg gcgattaaaa agttggggaa ggcatttcgt cttgaaacgg gggatgtttt gttctttgat acatccctgg aacacgggaa caccgaggtc cacaactttg attattgctg gaagcgggtg agcgttgtct gctacctgcg aaatggtctc atgtctcaaa tatgcgagat ggaacgacgc cagtggcttc aaaagcagat gttgaagcaa cgtcttctgg ataggagtcg ccagagtgtt ataaacctaa atgccacgga cccaaacctt cctcccatat accttcctgg aagattgctg gaggttctct cccccgttca acaagccgct cttggatttg ttgtggatcg acttagcaaa ggcaacgggt gtgtaattgc gctgacgatg ggattgggaa aaacgttgct ttccctggca ctgtgctatt cgcatatgta tgaccaaaat ccacgggatg tacttatttt ggcaccgaag attgttctta ctcattggac aggagaaaag caaaagtggg aaaagtacgg ccttgttttt tcacattttg tcgtctccga tgggacggac tctgtttcct ttgaaatcgc actcaagcga tataaacaac agctcaatgg ggagttgccc agaaccagtc atgtgtttgt catcaacccc gagtacatcc ggacggtcct gaaaaaactc accggatttc gtccaagtct tattattgtc gatgagggtc accgtgtgtc ctccaaggga agcaaattga aggactggtt ggagggactg cggtgcacgg cgcgtgtgat actctctgga acgcctgtgc agaacaatgc ggaggaatta taccgactca tcggctggat aaacagcgac gttcattctg ttctaccccc acgtgtgttt acggatcttg cggggactat caatcgttac attaatggag atgattcggc acttgccgca gccgtgtctg cgcaacgtta tattcaagag tggatgtgtt cctatgtttt tagcgtcatg aagacagacc ttccaccact taatgactat attattattt gtggattttc cagcattcaa aggaaaatgc ttgaggatca ttttgggatg gaagggatcg atgggctgac aagcataaag gcatctgagc accgtccata tcacctctcc acacatccgc tttgtttttt gggatttata tccggagtct ataaatcgct taatggaaat cataagttga cgcctgaggc ggaggaagag cttgagtcgc aggagtatgc atcgcagcta tattcactga ccgaagatga cattggactg attgatgagt gtttatcgct ggtgagctct ggtttcctaa cggagtttgt gggcttaagt gggaagatga ctgttctcat ttcgatattg cattcaattc gtgaaaaaaa ggagaaggcc atcatcttct ctcaatacgt cggatcacaa gactttatct cgcgaacatt gacttcattt gacatcgtct cctccaccat ccgcggaagg gactgtcatg aacgtcgaag acgaacaata gagaaattta gagaggatga gaacattact tgccttctct tatcaactca aattggcgcg tatggccttg acttcactgc cgcaaaccac gttatcctgt gggacagttg gtggaaccct caagtggagt cgcaggccat cgcacgagca tacagaagaa atcaaacacg agcagtcatt gtttaccgtc ttgcctcgga gttcgaggat acaattgtcc tcaaaacgca gataaggaaa ctggcactat tcagatgtat tatgaatgag gaagcatcac gtgcggtacc tcctgaagag ctgctggatt gcgttgacac cgaagaagat gagggaagac gctttttatg gcgttccctt aagaagagtt acctggaggg tggtgcaccg gcggtatcca aggtattccg tcatggcgac acggtgagaa gtgaatcatg gtcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for TrcrB.19528.a.B2.GE41513
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 18kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML QSVLQTVCST GEPAIIAPSS FLGELDTVVD EVKRHGMKLA SIPHGGITIL PPVPISGTEL FDFCAEYCKA QTHEERLAVR HKINNHNFLM QDPLLRMPCR QLYNPADYVL RIVHLCSELV SASEEEYHGA YGVAPLLHIN PVQDVCGKLR
Validated NT Sequence
atggctcacc accaccacca ccatatgctg cagtcggtat tacaaaccgt ttgctccaca ggggagccag ccataattgc cccgtcctct tttttgggag agctggacac cgtcgtcgat gaggtaaaaa gacatggaat gaaactggca agcattcctc atggtggaat cactattctt cctcctgttc caatcagtgg taccgaactc ttcgactttt gcgcagagta ttgtaaagct caaacgcacg aagagaggtt ggccgtgcgt cataaaataa ataaccataa tttcttaatg caggaccctc ttctgcggat gccctgtcga caactgtata acccagccga ctatgtgctg cgtatagttc acctctgctc agagcttgtt tctgcttcag aggaagagta ccacggggcc tacggcgtcg caccacttct tcatattaac cctgttcaag atgtttgcgg caagctgcgc taagtgagta agat
Expected Protein Sequence
MAHHHHHHLQ SVLQTVCSTG EPAIIAPSSF LGELDTVVDE VKRHGMKLAS IPHGGITILP PVPISGTELF DFCAEYCKAQ THEERLAVRH KINNHNFLMQ DPLLRMPCRQ LYNPADYVLR IVHLCSELVS ASEEEYHGAY GVAPLLHINP VQDVCGKLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgcagt cggtattaca aaccgtttgc tccacagggg agccagccat aattgccccg tcctcttttt tgggagagct ggacaccgtc gtcgatgagg taaaaagaca tggaatgaaa ctggcaagca ttcctcatgg tggaatcact attcttcctc ctgttccaat cagtggtacc gaactcttcg acttttgcgc agagtattgt aaagctcaaa cgcacgaaga gaggttggcc gtgcgtcata aaataaataa ccataatttc ttaatgcagg accctcttct gcggatgccc tgtcgacaac tgtataaccc agccgactat gtgctgcgta tagttcacct ctgctcagag cttgtttctg cttcagagga agagtaccac ggggcctacg gcgtcgcacc acttcttcat attaaccctg ttcaagatgt ttgcggcaag ctgcgctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for TrcrB.19528.a.B3.GE41514
HARVESTED ON: 4/21/2017
SEQUENCED ON: 5/3/2017
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD LTGQEVVDNA TDTVVEYLSE KDFDVVTESG IFYDDSGERV HAIYLRGGIK KELCQRAAIA IEEAATTKNL RKAVNGGKTN PETGIVGYYD YLNNPTQRKC RETEFTRKNW SSVVDSCEPF LVALNKLYSE CAPTHYKLQR IAIPRHYQLF NTVFSTMTVN RNFRTAVHTD RGDFRSGLAA LCVIDGVFEG CHLAIKKLGK AFRLETGDVL FFDTSLEHGN TEVHNFDYCW KRVSVVCYLR NGLMSQICEM ERRQWLQKQM LKQRL
Validated NT Sequence
atggctcacc accaccacca ccatatggat ctaactggac aggaggttgt tgacaacgcg acggatacag tggtggagta cctctccgaa aaggactttg acgtggtaac ggagagcggc atattctatg atgattctgg ggaacgggtg catgccattt atcttcgagg tggaattaag aaggaactct gtcagcgggc cgctattgcc attgaagaag ctgcgacgac caaaaatctg cgcaaagccg tcaatggagg gaaaacgaac ccggaaacgg gtattgtggg ctactatgac tacttgaata atcccacaca gcgcaagtgc cgtgagacag agtttacacg aaaaaattgg agttctgtag tcgactcctg cgagccattt cttgtggcac taaataaact ttacagtgag tgtgccccga cgcattacaa acttcaacgc attgctatac cacgccatta tcaattgttt aatacggttt ttagtacaat gacggttaat cgaaattttc gaactgctgt tcacacggat cggggtgact tccgcagtgg attggccgct ctctgtgtga tagacggggt ctttgaaggg tgccatttgg cgattaaaaa gttggggaag gcatttcgtc ttgaaacggg ggatgttttg ttctttgata catccctgga acacgggaac accgaggtcc acaactttga ttattgctgg aagcgggtga gcgttgtctg ctacctgcga aatggtctca tgtctcaaat atgcgagatg gaacgacgcc agtggcttca aaagcagatg ttgaagcaac gtctttaagt gagtaagat
Expected Protein Sequence
MAHHHHHHDL TGQEVVDNAT DTVVEYLSEK DFDVVTESGI FYDDSGERVH AIYLRGGIKK ELCQRAAIAI EEAATTKNLR KAVNGGKTNP ETGIVGYYDY LNNPTQRKCR ETEFTRKNWS SVVDSCEPFL VALNKLYSEC APTHYKLQRI AIPRHYQLFN TVFSTMTVNR NFRTAVHTDR GDFRSGLAAL CVIDGVFEGC HLAIKKLGKA FRLETGDVLF FDTSLEHGNT EVHNFDYCWK RVSVVCYLRN GLMSQICEME RRQWLQKQML KQRL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatctaa ctggacagga ggttgttgac aacgcgacgg atacagtggt ggagtacctc tccgaaaagg actttgacgt ggtaacggag agcggcatat tctatgatga ttctggggaa cgggtgcatg ccatttatct tcgaggtgga attaagaagg aactctgtca gcgggccgct attgccattg aagaagctgc gacgaccaaa aatctgcgca aagccgtcaa tggagggaaa acgaacccgg aaacgggtat tgtgggctac tatgactact tgaataatcc cacacagcgc aagtgccgtg agacagagtt tacacgaaaa aattggagtt ctgtagtcga ctcctgcgag ccatttcttg tggcactaaa taaactttac agtgagtgtg ccccgacgca ttacaaactt caacgcattg ctataccacg ccattatcaa ttgtttaata cggtttttag tacaatgacg gttaatcgaa attttcgaac tgctgttcac acggatcggg gtgacttccg cagtggattg gccgctctct gtgtgataga cggggtcttt gaagggtgcc atttggcgat taaaaagttg gggaaggcat ttcgtcttga aacgggggat gttttgttct ttgatacatc cctggaacac gggaacaccg aggtccacaa ctttgattat tgctggaagc gggtgagcgt tgtctgctac ctgcgaaatg gtctcatgtc tcaaatatgc gagatggaac gacgccagtg gcttcaaaag cagatgttga agcaacgtct ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for TrcrB.19528.a.B3.PS38310
PURIFICATION DATe: 6/29/2017
CONCENTRATION: 2.1mg/ml
OBSERVED MW: 31kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD LTGQEVVDNA TDTVVEYLSE KDFDVVTESG IFYDDSGERV HAIYLRGGIK KELCQRAAIA IEEAATTKNL RKAVNGGKTN PETGIVGYYD YLNNPTQRKC RETEFTRKNW SSVVDSCEPF LVALNKLYSE CAPTHYKLQR IAIPRHYQLF NTVFSTMTVN RNFRTAVHTD RGDFRSGLAA LCVIDGVFEG CHLAIKKLGK AFRLETGDVL FFDTSLEHGN TEVHNFDYCW KRVSVVCYLR NGLMSQICEM ERRQWLQKQM LKQRL
Validated NT Sequence
atggctcacc accaccacca ccatatggat ctaactggac aggaggttgt tgacaacgcg acggatacag tggtggagta cctctccgaa aaggactttg acgtggtaac ggagagcggc atattctatg atgattctgg ggaacgggtg catgccattt atcttcgagg tggaattaag aaggaactct gtcagcgggc cgctattgcc attgaagaag ctgcgacgac caaaaatctg cgcaaagccg tcaatggagg gaaaacgaac ccggaaacgg gtattgtggg ctactatgac tacttgaata atcccacaca gcgcaagtgc cgtgagacag agtttacacg aaaaaattgg agttctgtag tcgactcctg cgagccattt cttgtggcac taaataaact ttacagtgag tgtgccccga cgcattacaa acttcaacgc attgctatac cacgccatta tcaattgttt aatacggttt ttagtacaat gacggttaat cgaaattttc gaactgctgt tcacacggat cggggtgact tccgcagtgg attggccgct ctctgtgtga tagacggggt ctttgaaggg tgccatttgg cgattaaaaa gttggggaag gcatttcgtc ttgaaacggg ggatgttttg ttctttgata catccctgga acacgggaac accgaggtcc acaactttga ttattgctgg aagcgggtga gcgttgtctg ctacctgcga aatggtctca tgtctcaaat atgcgagatg gaacgacgcc agtggcttca aaagcagatg ttgaagcaac gtctttaagt gagtaagat
Expressed Protein Sequence
MAHHHHHHDL TGQEVVDNAT DTVVEYLSEK DFDVVTESGI FYDDSGERVH AIYLRGGIKK ELCQRAAIAI EEAATTKNLR KAVNGGKTNP ETGIVGYYDY LNNPTQRKCR ETEFTRKNWS SVVDSCEPFL VALNKLYSEC APTHYKLQRI AIPRHYQLFN TVFSTMTVNR NFRTAVHTDR GDFRSGLAAL CVIDGVFEGC HLAIKKLGKA FRLETGDVLF FDTSLEHGNT EVHNFDYCWK RVSVVCYLRN GLMSQICEME RRQWLQKQML KQRL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGATCTAA CTGGACAGGA GGTTGTTGAC AACGCGACGG ATACAGTGGT GGAGTACCTC TCCGAAAAGG ACTTTGACGT GGTAACGGAG AGCGGCATAT TCTATGATGA TTCTGGGGAA CGGGTGCATG CCATTTATCT TCGAGGTGGA ATTAAGAAGG AACTCTGTCA GCGGGCCGCT ATTGCCATTG AAGAAGCTGC GACGACCAAA AATCTGCGCA AAGCCGTCAA TGGAGGGAAA ACGAACCCGG AAACGGGTAT TGTGGGCTAC TATGACTACT TGAATAATCC CACACAGCGC AAGTGCCGTG AGACAGAGTT TACACGAAAA AATTGGAGTT CTGTAGTCGA CTCCTGCGAG CCATTTCTTG TGGCACTAAA TAAACTTTAC AGTGAGTGTG CCCCGACGCA TTACAAACTT CAACGCATTG CTATACCACG CCATTATCAA TTGTTTAATA CGGTTTTTAG TACAATGACG GTTAATCGAA ATTTTCGAAC TGCTGTTCAC ACGGATCGGG GTGACTTCCG CAGTGGATTG GCCGCTCTCT GTGTGATAGA CGGGGTCTTT GAAGGGTGCC ATTTGGCGAT TAAAAAGTTG GGGAAGGCAT TTCGTCTTGA AACGGGGGAT GTTTTGTTCT TTGATACATC CCTGGAACAC GGGAACACCG AGGTCCACAA CTTTGATTAT TGCTGGAAGC GGGTGAGCGT TGTCTGCTAC CTGCGAAATG GTCTCATGTC TCAAATATGC GAGATGGAAC GACGCCAGTG GCTTCAAAAG CAGATGTTGA AGCAACGTCT TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT