SSGCID: Target Page

TrcrB.19527.b

Glucosyl transferase (HmdUGT)

CENTER ID:	TrcrB.19527.b
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
TrcrB.19527.b.B1.GE41509	full length	1	637
TrcrB.19527.b.B2.GE41510	nucleoside diphospho-sugar transferase-like domain	6	405
TrcrB.19527.b.B3.GE41511	C-term domain	493	637

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19527.b.B3.PS38334	C-term domain		493	637

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.510187.10
UniProt:	Q4E4F0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MWNVRMRTLA LLEESFSGSP VRLVADGRND GGVGGIEKLN GHNEAYFCPV FIPSKARYHS ERGTISLLIQ DKVPFTLVVQ PEELTLYERL VDRLVNKWWG DVHNNGQGGT EDGTDGDELR FLFPRHGLQR NSTLLSQELL STRPCTPPPP PPLSRRRDAS DVRHLVKIHA LPLSDQGVSY VRNYILTELV PRALATSLPS SSSAPRAAQW FWVMDDDIQR FYMAWDEKNH VITPRELFTE VYSRIQELHG NICDMNGIAI FSLEYPRFAY TYGDSDVALN SYNNIASLFR YDLLPPDCKY RFRIREDYDY TLQLINHGMM TARFRNLSFE VPGMAEMAGG MTDYYKTQKE DIRLQNKLFL ETWPAVAQEC IKGKGALQRE DIRVRWDLLH PKRNPDPSAT LRCRTPMQKK AHKPLSNGID ETESMAKNGC ENTTTIGMST RNLKDKRTRS SEAARGANKQ RKEKQKQKMT LKRNTSLNGK EEEARRKRVN CGIKRQPPEW KGYALERWRY LTVHEAERLH LHLIPSEKLR VGQTVAVVPP MFDQNPSVVE AVLVDKRVMR RRRSERNGEQ GATDAKKGSE VDIVVEWCAV ARGLPLLYVT RCYELPMEGV DVAAAAVDAF FEKEWEEEEE EEDGKVE

NT Sequence

atgtggaatg tacgaatgcg gacccttgcg ctactggaag agagtttttc gggaagtcct gttcgcctcg ttgccgatgg tcgtaacgac ggtggtgtgg gaggcattga gaagttgaac gggcataatg aggcgtactt ctgccccgta tttattccat cgaaggcgcg ataccacagt gagcgcggga caatatcgct cctgatacaa gacaaggtgc ccttcacgct tgtcgtgcag ccagaggagc tcacgttgta cgaacggctg gtggatcggt tggttaataa gtggtggggt gatgtgcaca acaatggcca gggcggtacg gaggatggta cagacggtga tgagttgcgg ttcctgtttc cgcgtcatgg gttgcaacgc aattcaaccc tcttgtcgca ggagctgctc tccacacggc cctgtacgcc tccaccccca ccacccttgt cacgacgacg agatgcatcc gatgttcggc accttgttaa aattcatgca ttgccgcttt ccgatcaggg cgtctcttat gtgcggaatt acattttgac tgaacttgtt ccgagagctt tggcgacatc tttaccctct tcttcctccg cgccgcgggc agcacagtgg ttttgggtaa tggacgacga cattcaacgg ttctatatgg cgtgggatga aaagaaccat gtcatcactc cccgcgaact cttcactgag gtctacagtc gcattcaaga gttacatggt aacatctgcg acatgaatgg catcgctatc ttttcgctag aatacccccg ttttgcctac acttatggtg atagcgacgt tgctttgaac agctacaaca acattgcctc actgtttcgt tacgacctat tgccacccga ctgcaagtac cggttccgta tccgagagga ctatgactac acgctgcaac ttataaatca cgggatgatg actgcgcggt tccgtaatct ttcctttgag gtgcctggaa tggccgagat ggcggggggt atgacggact actacaagac ccagaaggaa gatatcagat tgcagaacaa gttgtttctc gagacttggc cggccgtggc acaggaatgc atcaagggaa agggggcatt gcagcgagaa gacatacgtg tccggtggga tctccttcac ccaaaaagga accctgaccc cagtgccact ttgcggtgcc gcacgccgat gcagaagaag gcacataagc ctttgtccaa tggcattgat gaaactgagt cgatggcgaa aaacggctgt gagaatacaa caacaattgg aatgagcaca agaaatttga aggacaagag gactcggtcg tcggaggcgg cgcgaggggc gaataagcag agaaaagaga agcagaagca gaaaatgacg ctgaagagaa acaccagttt aaatgggaaa gaagaagaag cgaggaggaa gagggtaaat tgtggaatca agcgacaacc accagagtgg aagggctacg ccctcgaaag gtggaggtat cttacagttc atgaggcgga gcgactccac ttgcacctta ttccatcaga aaagttgcgt gttgggcaaa ccgttgcagt cgtcccacca atgtttgatc agaacccctc tgttgtggaa gcggtacttg tcgacaagag agttatgcga aggcgccgat ctgaaaggaa cggggagcag ggcgccacgg atgccaaaaa gggatctgag gtggatatcg ttgttgagtg gtgtgcggtg gcacgtggcc tacctctcct gtacgtgact cgttgctacg aactgcctat ggagggtgtt gatgtcgccg cggccgccgt ggatgcgttc tttgagaagg aatgggaaga agaagaagaa gaggaggatg gtaaggtaga gtaa

Details for TrcrB.19527.b.B1.GE41509

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	74kDa
OBSERVED MW:	74kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMW NVRMRTLALL EESFSGSPVR LVADGRNDGG VGGIEKLNGH NEAYFCPVFI PSKARYHSER GTISLLIQDK VPFTLVVQPE ELTLYERLVD RLVNKWWGDV HNNGQGGTED GTDGDELRFL FPRHGLQRNS TLLSQELLST RPCTPPPPPP LSRRRDASDV RHLVKIHALP LSDQGVSYVR NYILTELVPR ALATSLPSSS SAPRAAQWFW VMDDDIQRFY MAWDEKNHVI TPRELFTEVY SRIQELHGNI CDMNGIAIFS LEYPRFAYTY GDSDVALNSY NNIASLFRYD LLPPDCKYRF RIREDYDYTL QLINHGMMTA RFRNLSFEVP GMAEMAGGMT DYYKTQKEDI RLQNKLFLET WPAVAQECIK GKGALQREDI RVRWDLLHPK RNPDPSATLR CRTPMQKKAH KPLSNGIDET ESMAKNGCEN TTTIGMSTRN LKDKRTRSSE AARGANKQRK EKQKQKMTLK RNTSLNGKEE EARRKRVNCG IKRQPPEWKG YALERWRYLT VHEAERLHLH LIPSEKLRVG QTVAVVPPMF DQNPSVVEAV LVDKRVMRRR RSERNGEQGA TDAKKGSEVD IVVEWCAVAR GLPLLYVTRC YELPMEGVDV AAAAVDAFFE KEWEEEEEEE DGKVE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgga atgtacgaat gcggaccctt gcgctactgg aagagagttt ttcgggaagt cctgttcgcc tcgttgccga tggtcgtaac gacggtggtg tgggaggcat tgagaagttg aacgggcata atgaggcgta cttctgcccc gtatttattc catcgaaggc gcgataccac agtgagcgcg ggacaatatc gctcctgata caagacaagg tgcccttcac gcttgtcgtg cagccagagg agctcacgtt gtacgaacgg ctggtggatc ggttggttaa taagtggtgg ggtgatgtgc acaacaatgg ccagggcggt acggaggatg gtacagacgg tgatgagttg cggttcctgt ttccgcgtca tgggttgcaa cgcaattcaa ccctcttgtc gcaggagctg ctctccacac ggccctgtac gcctccaccc ccaccaccct tgtcacgacg acgagatgca tccgatgttc ggcaccttgt taaaattcat gcattgccgc tttccgatca gggcgtctct tatgtgcgga attacatttt gactgaactt gttccgagag ctttggcgac atctttaccc tcttcttcct ccgcgccgcg ggcagcacag tggttttggg taatggacga cgacattcaa cggttctata tggcgtggga tgaaaagaac catgtcatca ctccccgcga actcttcact gaggtctaca gtcgcattca agagttacat ggtaacatct gcgacatgaa tggcatcgct atcttttcgc tagaataccc ccgttttgcc tacacttatg gtgatagcga cgttgctttg aacagctaca acaacattgc ctcactgttt cgttacgacc tattgccacc cgactgcaag taccggttcc gtatccgaga ggactatgac tacacgctgc aacttataaa tcacgggatg atgactgcgc ggttccgtaa tctttccttt gaggtgcctg gaatggccga gatggcgggg ggtatgacgg actactacaa gacccagaag gaagatatca gattgcagaa caagttgttt ctcgagactt ggccggccgt ggcacaggaa tgcatcaagg gaaagggggc attgcagcga gaagacatac gtgtccggtg ggatctcctt cacccaaaaa ggaaccctga ccccagtgcc actttgcggt gccgcacgcc gatgcagaag aaggcacata agcctttgtc caatggcatt gatgaaactg agtcgatggc gaaaaacggc tgtgagaata caacaacaat tggaatgagc acaagaaatt tgaaggacaa gaggactcgg tcgtcggagg cggcgcgagg ggcgaataag cagagaaaag agaagcagaa gcagaaaatg acgctgaaga gaaacaccag tttaaatggg aaagaagaag aagcgaggag gaagagggta aattgtggaa tcaagcgaca accaccagag tggaagggct acgccctcga aaggtggagg tatcttacag ttcatgaggc ggagcgactc cacttgcacc ttattccatc agaaaagttg cgtgttgggc aaaccgttgc agtcgtccca ccaatgtttg atcagaaccc ctctgttgtg gaagcggtac ttgtcgacaa gagagttatg cgaaggcgcc gatctgaaag gaacggggag cagggcgcca cggatgccaa aaagggatct gaggtggata tcgttgttga gtggtgtgcg gtggcacgtg gcctacctct cctgtacgtg actcgttgct acgaactgcc tatggagggt gttgatgtcg ccgcggccgc cgtggatgcg ttctttgaga aggaatggga agaagaagaa gaagaggagg atggtaaggt agagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for TrcrB.19527.b.B2.GE41510

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR TLALLEESFS GSPVRLVADG RNDGGVGGIE KLNGHNEAYF CPVFIPSKAR YHSERGTISL LIQDKVPFTL VVQPEELTLY ERLVDRLVNK WWGDVHNNGQ GGTEDGTDGD ELRFLFPRHG LQRNSTLLSQ ELLSTRPCTP PPPPPLSRRR DASDVRHLVK IHALPLSDQG VSYVRNYILT ELVPRALATS LPSSSSAPRA AQWFWVMDDD IQRFYMAWDE KNHVITPREL FTEVYSRIQE LHGNICDMNG IAIFSLEYPR FAYTYGDSDV ALNSYNNIAS LFRYDLLPPD CKYRFRIRED YDYTLQLINH GMMTARFRNL SFEVPGMAEM AGGMTDYYKT QKEDIRLQNK LFLETWPAVA QECIKGKGAL QREDIRVRWD LLHPKRNPDP

Validated NT Sequence

atggctcacc accaccacca ccatatgcgg acccttgcgc tactggaaga gagtttttcg ggaagtcctg ttcgcctcgt tgccgatggt cgtaacgacg gtggtgtggg aggcattgag aagttgaacg ggcataatga ggcgtacttc tgccccgtat ttattccatc gaaggcgcga taccacagtg agcgcgggac aatatcgctc ctgatacaag acaaggtgcc cttcacgctt gtcgtgcagc cagaggagct cacgttgtac gaacggctgg tggatcggtt ggttaataag tggtggggtg atgtgcacaa caatggccag ggcggtacgg aggatggtac agacggtgat gagttgcggt tcctgtttcc gcgtcatggg ttgcaacgca attcaaccct cttgtcgcag gagctgctct ccacacggcc ctgtacgcct ccacccccac cacccttgtc acgacgacga gatgcatccg atgttcggca ccttgttaaa attcatgcat tgccgctttc cgatcagggc gtctcttatg tgcggaatta cattttgact gaacttgttc cgagagcttt ggcgacatct ttaccctctt cttcctccgc gccgcgggca gcacagtggt tttgggtaat ggacgacgac attcaacggt tctatatggc gtgggatgaa aagaaccatg tcatcactcc ccgcgaactc ttcactgagg tctacagtcg cattcaagag ttacatggta acatctgcga catgaatggc atcgctatct tttcgctaga atacccccgt tttgcctaca cttatggtga tagcgacgtt gctttgaaca gctacaacaa cattgcctca ctgtttcgtt acgacctatt gccacccgac tgcaagtacc ggttccgtat ccgagaggac tatgactaca cgctgcaact tataaatcac gggatgatga ctgcgcggtt ccgtaatctt tcctttgagg tgcctggaat ggccgagatg gcggggggta tgacggacta ctacaagacc cagaaggaag atatcagatt gcagaacaag ttgtttctcg agacttggcc ggccgtggca caggaatgca tcaagggaaa gggggcattg cagcgagaag acatacgtgt ccggtgggat ctccttcacc caaaaaggaa ccctgacccc a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgga cccttgcgct actggaagag agtttttcgg gaagtcctgt tcgcctcgtt gccgatggtc gtaacgacgg tggtgtggga ggcattgaga agttgaacgg gcataatgag gcgtacttct gccccgtatt tattccatcg aaggcgcgat accacagtga gcgcgggaca atatcgctcc tgatacaaga caaggtgccc ttcacgcttg tcgtgcagcc agaggagctc acgttgtacg aacggctggt ggatcggttg gttaataagt ggtggggtga tgtgcacaac aatggccagg gcggtacgga ggatggtaca gacggtgatg agttgcggtt cctgtttccg cgtcatgggt tgcaacgcaa ttcaaccctc ttgtcgcagg agctgctctc cacacggccc tgtacgcctc cacccccacc acccttgtca cgacgacgag atgcatccga tgttcggcac cttgttaaaa ttcatgcatt gccgctttcc gatcagggcg tctcttatgt gcggaattac attttgactg aacttgttcc gagagctttg gcgacatctt taccctcttc ttcctccgcg ccgcgggcag cacagtggtt ttgggtaatg gacgacgaca ttcaacggtt ctatatggcg tgggatgaaa agaaccatgt catcactccc cgcgaactct tcactgaggt ctacagtcgc attcaagagt tacatggtaa catctgcgac atgaatggca tcgctatctt ttcgctagaa tacccccgtt ttgcctacac ttatggtgat agcgacgttg ctttgaacag ctacaacaac attgcctcac tgtttcgtta cgacctattg ccacccgact gcaagtaccg gttccgtatc cgagaggact atgactacac gctgcaactt ataaatcacg ggatgatgac tgcgcggttc cgtaatcttt cctttgaggt gcctggaatg gccgagatgg cggggggtat gacggactac tacaagaccc agaaggaaga tatcagattg cagaacaagt tgtttctcga gacttggccg gccgtggcac aggaatgcat caagggaaag ggggcattgc agcgagaaga catacgtgtc cggtgggatc tccttcaccc aaaaaggaac cctgacccca gtgccacttt gcggtgccgc acgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for TrcrB.19527.b.B3.GE41511

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	18kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI KRQPPEWKGY ALERWRYLTV HEAERLHLHL IPSEKLRVGQ TVAVVPPMFD QNPSVVEAVL VDKRVMRRRR SERNGEQGAT DAKKGSEVDI VVEWCAVARG LPLLYVTRCY ELPMEGVDVA AAAVDAFFEK EWEEEEEEED GKVE

Validated NT Sequence

atggctcacc accaccacca ccatatgatc aagcgacaac caccagagtg gaagggctac gccctcgaaa ggtggaggta tcttacagtt catgaggcgg agcgactcca cttgcacctt attccatcag aaaagttgcg tgttgggcaa accgttgcag tcgtcccacc aatgtttgat cagaacccct ctgttgtgga agcggtactt gtcgacaaga gagttatgcg aaggcgccga tctgaaagga acggggagca gggcgccacg gatgccaaaa agggatctga ggtggatatc gttgttgagt ggtgtgcggt ggcacgtggc ctacctctcc tgtacgtgac tcgttgctac gaactgccta tggagggtgt tgatgtcgcc gcggccgccg tggatgcgtt ctttgagaag gaatgggaag aagaagaaga agaggaggat ggtaaggtag agtaagtgag taagat

Expected Protein Sequence

MAHHHHHHIK RQPPEWKGYA LERWRYLTVH EAERLHLHLI PSEKLRVGQT VAVVPPMFDQ NPSVVEAVLV DKRVMRRRRS ERNGEQGATD AKKGSEVDIV VEWCAVARGL PLLYVTRCYE LPMEGVDVAA AAVDAFFEKE WEEEEEEEDG KVE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcaagc gacaaccacc agagtggaag ggctacgccc tcgaaaggtg gaggtatctt acagttcatg aggcggagcg actccacttg caccttattc catcagaaaa gttgcgtgtt gggcaaaccg ttgcagtcgt cccaccaatg tttgatcaga acccctctgt tgtggaagcg gtacttgtcg acaagagagt tatgcgaagg cgccgatctg aaaggaacgg ggagcagggc gccacggatg ccaaaaaggg atctgaggtg gatatcgttg ttgagtggtg tgcggtggca cgtggcctac ctctcctgta cgtgactcgt tgctacgaac tgcctatgga gggtgttgat gtcgccgcgg ccgccgtgga tgcgttcttt gagaaggaat gggaagaaga agaagaagag gaggatggta aggtagagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for TrcrB.19527.b.B3.PS38334

PURIFICATION DATe:	8/8/2017
CONCENTRATION:	28.15mg/ml
OBSERVED MW:	18kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	12
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMI KRQPPEWKGY ALERWRYLTV HEAERLHLHL IPSEKLRVGQ TVAVVPPMFD QNPSVVEAVL VDKRVMRRRR SERNGEQGAT DAKKGSEVDI VVEWCAVARG LPLLYVTRCY ELPMEGVDVA AAAVDAFFEK EWEEEEEEED GKVE

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHIK RQPPEWKGYA LERWRYLTVH EAERLHLHLI PSEKLRVGQT VAVVPPMFDQ NPSVVEAVLV DKRVMRRRRS ERNGEQGATD AKKGSEVDIV VEWCAVARGL PLLYVTRCYE LPMEGVDVAA AAVDAFFEKE WEEEEEEEDG KVE

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATCAAGC GACAACCACC AGAGTGGAAG GGCTACGCCC TCGAAAGGTG GAGGTATCTT ACAGTTCATG AGGCGGAGCG ACTCCACTTG CACCTTATTC CATCAGAAAA GTTGCGTGTT GGGCAAACCG TTGCAGTCGT CCCACCAATG TTTGATCAGA ACCCCTCTGT TGTGGAAGCG GTACTTGTCG ACAAGAGAGT TATGCGAAGG CGCCGATCTG AAAGGAACGG GGAGCAGGGC GCCACGGATG CCAAAAAGGG ATCTGAGGTG GATATCGTTG TTGAGTGGTG TGCGGTGGCA CGTGGCCTAC CTCTCCTGTA CGTGACTCGT TGCTACGAAC TGCCTATGGA GGGTGTTGAT GTCGCCGCGG CCGCCGTGGA TGCGTTCTTT GAGAAGGAAT GGGAAGAAGA AGAAGAAGAG GAGGATGGTA AGGTAGAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT