TrcrB.19519.a

KREPB10

CENTER ID: TrcrB.19519.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.19519.a.B1.GE40819 full length 1 366
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TcCLB.509109.150
RefSeq: XP_816681.1
UniProt: Q4DQM1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPRRSKAER TFLAELASQS TGATAISLLS SCTSSNGKNN LRVGLRGSSP MCRSTFAQEG HPCWLQNRIF DAPNKHLFLD PGGTWCHLCR EPLGTSAVGH LSDREHHNHQ LFLFLAAAFP RGVHGEHQRF LYSPLAVLDD AAVLFPKLMQ AVGEGNMHTE QDALRRASLE SMLIYLSDGG LCGGKPVLTV FHGSVPPELA HSGERLFKSD VSRLIVEWFP ALGPGAITQI AHKCWGRSNL ESIFDALRMQ SLVDRRGREV AGVLSPLARN RAWRTTLQRQ LSTKTEKATF VRLLMWELTT RCEEAAGCDA ALPLTETEQL MLELTRRRLA FEMVYLQSMK YMQHADQLLR EMLFPSLETL ISMGAA
NT Sequence
atgcggccgc ggcgttccaa ggcagagagg acgtttttgg ccgagttggc ctcgcagagc accggtgcca ccgccatttc tttgctttcc tcctgcacct cgtcgaatgg aaagaataat ctgcgtgtgg ggctgcgtgg cagcagcccg atgtgtcgca gcacctttgc acaggagggc catccctgtt ggcttcagaa ccgcatcttt gacgcaccaa acaagcatct ctttttggat cctggcggca cgtggtgtca tttgtgccgc gagcccctag gaacttccgc ggtcggccat ctgtctgacc gtgagcacca caaccatcaa ctttttttgt ttcttgcagc cgcatttcct cggggggtgc atggagaaca tcagaggttc ttgtattcac cacttgctgt tttggatgat gcggcggtgt tgtttccaaa actcatgcag gcagtgggcg aaggtaatat gcatacggag caggatgcct tgcgacgtgc ctcactggag tcgatgctta tttacttgag tgacggtggc ttgtgcgggg ggaagccggt attgacggtg tttcatggca gcgttccccc cgagttggcg cacagcgggg agcgtttgtt taagagcgat gtgtcacggc tcatcgtgga gtggttcccg gcactgggac ctggtgccat cacgcagatt gcccacaaat gttggggaag gtcgaatttg gaaagtattt ttgatgcgct tcgaatgcag tcacttgtag accgacgagg ccgtgaggtg gctggggttt tatcaccgct ggcaagaaac agggcatgga gaaccacact tcagcgacaa ctttccacaa aaaccgaaaa ggccactttt gtgcgtttgc tgatgtggga gctgacaacg cggtgtgaag aggcagcggg ctgtgatgct gcattgccgc tcacagagac tgaacagttg atgcttgagc tcacgaggcg ccggctagcg tttgagatgg tgtacttgca gtccatgaag tacatgcagc acgcggatca attactgcga gagatgttgt tcccatccct tgagacgctc atatcaatgg gcgccgcgta g
Details for TrcrB.19519.a.B1.GE40819
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHHH MRPRRSKAER TFLAELASQS TGATAISLLS SCTSSNGKNN LRVGLRGSSP MCRSTFAQDG HPCWLQNRIF DAPNKHLFLD PGGTWCHLCR EPLGTSAVGH LSDREHHNHQ LFLFLAAAFP RGVHGEHQRL LYSPHAVLDD AAVLFPKLMQ AVGEGNMHTE QDALRRASLE SMLIYLSDGG LCGGKPVLTV FHGSVPPELA HSGERLFKSD VSRLIVEWFP ALGPGAITQI AHKCWGRSNL ESIFDALRMQ SLVDRRGREV AGVLSPLARN RAWRSTLQRQ LSTKTEKATF VRSLMWELTT RCEEAAGCDA ALPLTETEQL MLELTRRRLA FEMVYLQSMK YMQHADQLLR EMLFPSLE
Validated NT Sequence
cgtctcaagg gatgggaaca acatctctcg cagtaattga tccgcgtgct gcatgtactt catggactgc aagtacacca tctcaaacgc tagccggcgc ctcgtgagct caagcatcaa ctgttcagtc tctgtgagcg gcaatgcagc atcacagccc gctgcctctt cacaccgcgt tgtcagctcc cacatcagcg aacgcacaaa agtggccttt tcggtttttg tggaaagttg tcgctgaagt gtgcttctcc atgccctatt tcttgccagc ggtgataaaa ccccagccac ctcacggcct cgtcggtcta caagtgactg cattcgaagc gcatcaaaaa tgctctccaa attcgacctt ccccaacatt tgtgggcaat ctgcgtgatg gcaccaggtc ccagtgccgg gaaccactcc acgatgagcc gtgacacatc gctcttaaac agacgctccc cgctgtgcgc caactcgggg gggacgctgc catgaaacac cgtcaatacc ggcttccccc cgcacaagcc accgtcactc aagtaaataa gcatcgactc cagtgaggca cgtcgcaagg catcctgctc cgtatgcata ttaccttcgc ctactgcctg catgagtttt ggaaacaaca ccgccgcatc atccaaaaca gcatgtggtg aatacaagag cctctgatgt tctccatgca ccccgcgagg aaatgcggct gcaagaaaca aaaaaagttg atggttgtgg tgctcacggt cagatagatg gccgaccgcg gaagttccta ggggctcgcg gcacaaatga caccatgtgc cgccaggatc caaaaagaga tgcttgtttg gtgcgtcaaa gatgcggttc tgaagccaac agggatggcc gtcctgtgca aaggtgctgc gacacatcgg gctgctgcca cgcagcccca cacgcagatt gttctttcca ttcgacgagg tgcaagagga aagcaaagaa atggctgtgg caccggtgct ctgcgaggcc aactcggcca aaaacgtcct ctctgcctta gaacgccgcg gccgcatatg gtggtggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMR PRRSKAERTF LAELASQSTG ATAISLLSSC TSSNGKNNLR VGLRGSSPMC RSTFAQEGHP CWLQNRIFDA PNKHLFLDPG GTWCHLCREP LGTSAVGHLS DREHHNHQLF LFLAAAFPRG VHGEHQRFLY SPLAVLDDAA VLFPKLMQAV GEGNMHTEQD ALRRASLESM LIYLSDGGLC GGKPVLTVFH GSVPPELAHS GERLFKSDVS RLIVEWFPAL GPGAITQIAH KCWGRSNLES IFDALRMQSL VDRRGREVAG VLSPLARNRA WRTTLQRQLS TKTEKATFVR LLMWELTTRC EEAAGCDAAL PLTETEQLML ELTRRRLAFE MVYLQSMKYM QHADQLLREM LFPSLETLIS MGAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcggc cgcggcgttc caaggcagag aggacgtttt tggccgagtt ggcctcgcag agcaccggtg ccaccgccat ttctttgctt tcctcctgca cctcgtcgaa tggaaagaat aatctgcgtg tggggctgcg tggcagcagc ccgatgtgtc gcagcacctt tgcacaggag ggccatccct gttggcttca gaaccgcatc tttgacgcac caaacaagca tctctttttg gatcctggcg gcacgtggtg tcatttgtgc cgcgagcccc taggaacttc cgcggtcggc catctgtctg accgtgagca ccacaaccat caactttttt tgtttcttgc agccgcattt cctcgggggg tgcatggaga acatcagagg ttcttgtatt caccacttgc tgttttggat gatgcggcgg tgttgtttcc aaaactcatg caggcagtgg gcgaaggtaa tatgcatacg gagcaggatg ccttgcgacg tgcctcactg gagtcgatgc ttatttactt gagtgacggt ggcttgtgcg gggggaagcc ggtattgacg gtgtttcatg gcagcgttcc ccccgagttg gcgcacagcg gggagcgttt gtttaagagc gatgtgtcac ggctcatcgt ggagtggttc ccggcactgg gacctggtgc catcacgcag attgcccaca aatgttgggg aaggtcgaat ttggaaagta tttttgatgc gcttcgaatg cagtcacttg tagaccgacg aggccgtgag gtggctgggg ttttatcacc gctggcaaga aacagggcat ggagaaccac acttcagcga caactttcca caaaaaccga aaaggccact tttgtgcgtt tgctgatgtg ggagctgaca acgcggtgtg aagaggcagc gggctgtgat gctgcattgc cgctcacaga gactgaacag ttgatgcttg agctcacgag gcgccggcta gcgtttgaga tggtgtactt gcagtccatg aagtacatgc agcacgcgga tcaattactg cgagagatgt tgttcccatc ccttgagacg ctcatatcaa tgggcgccgc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat