SSGCID: Target Page

TrcrB.19518.a

KREPA5

CENTER ID:	TrcrB.19518.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19518.a.B1.GE40818	full length		1	164

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TcCLB.509611.40
RefSeq:	XP_814793.1
UniProt:	Q4DKA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFTSTWRSLA PRPLNRVTLL GAMHDVQRGF LGQSSVFQFT LTCLLFEPPK RDQAEKKPVA TPPSPTAKGG ASQFVKEQYT VRCMGDEAYT EALNNTLEAG CIVRVIGRLK TFEVAEGGKK QLFPTILVEQ GRWSHVALLH SLRRQRRDWQ LQNALTETRN LKQP

NT Sequence

atgtttacat cgacgtggag gtccctggcg cctcggccac tgaatcgcgt gacgctgctt ggcgcgatgc atgacgtgca gagggggttt cttggtcaat ccagtgtttt tcagtttact ctgacatgcc tgctttttga gcccccaaag cgggaccagg cagaaaagaa gcctgtcgcg acgcccccat ctccaacagc gaaagggggc gcgtcgcagt ttgtgaagga gcagtacact gttcgctgca tgggggatga ggcgtacacg gaggcgctga acaacacact ggaggcgggc tgtattgtgc gggtgattgg gcggctgaaa acatttgagg tggcggaggg cgggaaaaaa cagttgtttc caacaattct tgtagaacag ggacggtgga gtcatgtcgc actcctgcat tcattgagaa gacagcggcg tgattggcaa ctgcagaatg ctttgacaga gactcggaat ttaaagcagc cgtag

Details for TrcrB.19518.a.B1.GE40818

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	20kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHHM FTSTWRSLAP RPLNRVTLLG AMHDVQRGFL GQSSVFQFTL TCLLFEPPKR DQAEKKPVAT PPSPTAKGGA SQFVKEQYTV RCMGDEAYTE ALNNTLEAGC IVRVIGRLKT FEVAEGGKKQ LFPTILVEQG RWSHVALLHS LRRQRRDWQL QNALTETRNL KQP

Validated NT Sequence

atcttactca cttacggctg ctttaaattc cgagtctctg tcaaagcatt ctgcagttgc caatcacgcc gctgtcttct caatgaatgc aggagtgcga catgactcca ccgtccctgt tctacaagaa ttgttggaaa caactgtttt ttcccgccct ccgccacctc aaatgttttc agccgcccaa tcacccgcac aatacagccc gcctccagtg tgttgttcag cgcctccgtg tacgcctcat cccccatgca gcgaacagtg tactgctcct tcacaaactg cgacgcgccc cctttcgctg ttggagatgg gggcgtcgcg acaggcttct tttctgcctg gtcccgcttt gggggctcaa aaagcaggca tgtcagagta aactgaaaaa cactggattg accaagaaac cccctctgca cgtcatgcat cgcgccaagc agcgtcacgc gattcagtgg ccgaggcgcc agggacctcc acgtcgatgt aaacatatgg tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMF TSTWRSLAPR PLNRVTLLGA MHDVQRGFLG QSSVFQFTLT CLLFEPPKRD QAEKKPVATP PSPTAKGGAS QFVKEQYTVR CMGDEAYTEA LNNTLEAGCI VRVIGRLKTF EVAEGGKKQL FPTILVEQGR WSHVALLHSL RRQRRDWQLQ NALTETRNLK QP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta catcgacgtg gaggtccctg gcgcctcggc cactgaatcg cgtgacgctg cttggcgcga tgcatgacgt gcagaggggg tttcttggtc aatccagtgt ttttcagttt actctgacat gcctgctttt tgagccccca aagcgggacc aggcagaaaa gaagcctgtc gcgacgcccc catctccaac agcgaaaggg ggcgcgtcgc agtttgtgaa ggagcagtac actgttcgct gcatggggga tgaggcgtac acggaggcgc tgaacaacac actggaggcg ggctgtattg tgcgggtgat tgggcggctg aaaacatttg aggtggcgga gggcgggaaa aaacagttgt ttccaacaat tcttgtagaa cagggacggt ggagtcatgt cgcactcctg cattcattga gaagacagcg gcgtgattgg caactgcaga atgctttgac agagactcgg aatttaaagc agccgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat