SSGCID: Target Page

TrcrB.19517.a

KREPB9

CENTER ID:	TrcrB.19517.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19517.a.B1.GE40817	full length		1	444

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TcCLB.510155.60
RefSeq:	XP_820111.1
UniProt:	Q4E0H0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPSRRFNARR GMIAARRGFF TATSPCRQSR LVPFHELFAP LSSSSLPSSP RRFPVHSVCV DPPLGTLGEP SRSQGAGAED GSPLSWLREN LQLLEDGRKS LNERRASMPE GSPQWPRYMF PSHCCEYIYD PSNDSKLAFV TSDNYCHLCH EPVEVAIQHC AWWDHVTRLA AIRLIAIYPR RWSPVAVLRE ANEVLPTAVM VSPRPHPFLS FSEAVDTDDV SLMDMSSCYV FEREAVVRRA ELAAILRVLC TAKDADSCDD AEQRISSIIP VLRESLFLSA NGTPAASSGE RTFRAYISRQ ISVFLPPMAP EPTTRIQQRC WGRKNLEIMF DLLGVASLQR IAGAPVEAVT KNEKAAVLRQ IIYELATVLS EGDAEFAETS CAKINGTSSV DAEVQTRLLA EMALQRLAHE LIHLHTMLLM DQAYETYVKL GYPSEGMLEK SGFC

NT Sequence

atgccgtcac gaaggttcaa cgcacgccgt gggatgattg cagcgcgtcg tggattcttc acggccacgt cgccgtgccg tcaatctcga ttggttcctt ttcacgaact ttttgcgccg ctgtcgtcgt cttctttgcc gagctcgcca aggcggttcc cggttcacag cgtttgcgtg gatcctccat tgggcacatt gggggagccc tcacggagcc aaggagcggg ggcggaggac ggcagtccgc tcagttggct ccgtgaaaat cttcagcttt tggaagatgg gaggaaatcc cttaatgagc gtagggcgag catgccagag ggaagcccac agtggccgcg ctacatgttt ccctctcatt gctgtgaata tatatacgat ccttcaaacg attcaaagct tgcatttgtg acatcagaca attactgcca cttatgccac gaaccggtgg aggtggcgat tcaacattgc gcctggtggg accacgtgac acggctggca gccattcgtc ttatcgcaat ttacccccgc cgttggagtc ccgttgccgt cctacgggag gcgaacgaag tcctccccac tgccgtcatg gtgtcacccc ggccgcatcc atttttgtcc ttttcggaag cggtagatac cgatgatgtc tctttgatgg acatgagctc ttgttatgtt tttgaaaggg aggcggttgt gcggcgagcc gagttggcgg caatcctgcg tgtcttgtgt accgcaaagg acgccgacag ctgtgatgat gcggagcagc gtatctcctc gatcattcct gtgcttcgag agtcgctgtt tctttcagca aacggcactc ctgcggcaag ctcgggggag cggacctttc gtgcctacat tagccggcaa atcagtgtgt ttctaccgcc catggcaccg gagcccacga cgcggataca gcaacgttgt tgggggcgga aaaacttgga gatcatgttt gacctgctcg gtgtggcgtc actgcagaga attgccggag cgcctgttga ggcagtgaca aagaatgaga aggcggcagt ccttcggcaa atcatttacg aactagcgac ggtcctttcg gagggtgacg cggaattcgc agaaacttct tgcgccaaaa taaacggcac gtcctcagtc gatgcggagg tgcaaacccg tttgcttgca gagatggcgc tgcagcgcct cgcacatgag ttgattcatc ttcacacgat gttgctgatg gatcaggcgt atgaaaccta tgtgaagctg ggatacccga gcgaggggat gctggagaag agtggcttct gctga

Details for TrcrB.19517.a.B1.GE40817

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMP SRRFNARRGM IAARRGFFTA TSPCRQSRLV PFHELFAPLS SSSLPSSPRR FPVHSVCVDP PLGTLGEPSR SQGAGAEDGS PLSWLRENLQ LLEDGRKSLN ERRASMPEGS PQWPRYMFPS HCCEYIYDPS NDSKLAFVTS DNYCHLCHEP VEVAIQHCAW WDHVTRLAAI RLIAIYPRRW SPVAVLREAN EVLPTAVMVS PRPHPFLSFS EAVDTDDVSL MDMSSCYVFE REAVVRRAEL AAILRVLCTA KDADSCDDAE QRISSIIPVL RESLFLSANG TPAASSGERT FRAYISRQIS VFLPPMAPEP TTRIQQRCWG RKNLEIMFDL LGVASLQRIA GAPVEAVTKN EKAAVLRQII YELATVLSEG DAEFAETSCA KINGTSSVDA EVQTRLLAEM ALQRLAHELI HLHTMLLMDQ AYETYVKLGY PSEG

Validated NT Sequence

atcccctcgc tcgggtatcc cagcttcaca taggtttcat acgcctgatc catcagcaac atcgtgtgaa gatgaatcaa ctcatgtgcg aggcgctgca gcgccatctc tgcaagcaaa cgggtttgca cctccgcatc gactgaggac gtgccgttta ttttggcgca agaagtttct gcgaattccg cgtcaccctc cgaaaggacc gtcgctagtt cgtaaatgat ttgccgaagg actgccgcct tctcattctt tgtcactgcc tcaacaggcg ctccggcaat tctctgcagt gacgccacac cgagcaggtc aaacatgatc tccaagtttt tccgccccca acaacgttgc tgtatccgcg tcgtgggctc cggtgccatg ggcggtagaa acacactgat ttgccggcta atgtaggcac gaaaggtccg ctcccccgag cttgccgcag gagtgccgtt tgctgaaaga aacagcgact ctcgaagcac aggaatgatc gaggagatac gctgctccgc atcatcacag ctgtcggcgt cctttgcggt acacaagaca cgcaggattg ccgccaactc ggctcgccgc acaaccgcct ccctttcaaa aacataacaa gagctcatgt ccatcaaaga gacatcatcg gtatctaccg cttccgaaaa ggacaaaaat ggatgcggcc ggggtgacac catgacggca gtggggagga cttcgttcgc ctcccgtagg acggcaacgg gactccaacg gcgggggtaa attgcgataa gacgaatggc tgccagccgt gtcacgtggt cccaccaggc gcaatgttga atcgccacct ccaccggttc gtggcataag tggcagtaat tgtctgatgt cacaaatgca agctttgaat cgtttgaagg atcgtatata tattcacagc aatgagaggg aaacatgtag cgcggccact gtgggcttcc ctctggcatg ctcgccctac gctcattaag ggatttcctc ccatcttcca aaagctgaag attttcacgg agccaactga gcggactgcc gtcctccgcc cccgctcctt ggctccgtga gggctccccc aatgtgccca atggaggatc cacgcaaacg ctgtgaaccg ggaaccgcct tggcgagctc ggcaaagaag acgacgacag cggcgcaaaa agttcgtgaa aaggaaccaa tcgagattga cggcacggcg acgtggccgt gaagaatcca cgacgcgctg caatcatccc acggcgtgcg ttgaaccttc gtgacggcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgt cacgaaggtt caacgcacgc cgtgggatga ttgcagcgcg tcgtggattc ttcacggcca cgtcgccgtg ccgtcaatct cgattggttc cttttcacga actttttgcg ccgctgtcgt cgtcttcttt gccgagctcg ccaaggcggt tcccggttca cagcgtttgc gtggatcctc cattgggcac attgggggag ccctcacgga gccaaggagc gggggcggag gacggcagtc cgctcagttg gctccgtgaa aatcttcagc ttttggaaga tgggaggaaa tcccttaatg agcgtagggc gagcatgcca gagggaagcc cacagtggcc gcgctacatg tttccctctc attgctgtga atatatatac gatccttcaa acgattcaaa gcttgcattt gtgacatcag acaattactg ccacttatgc cacgaaccgg tggaggtggc gattcaacat tgcgcctggt gggaccacgt gacacggctg gcagccattc gtcttatcgc aatttacccc cgccgttgga gtcccgttgc cgtcctacgg gaggcgaacg aagtcctccc cactgccgtc atggtgtcac cccggccgca tccatttttg tccttttcgg aagcggtaga taccgatgat gtctctttga tggacatgag ctcttgttat gtttttgaaa gggaggcggt tgtgcggcga gccgagttgg cggcaatcct gcgtgtcttg tgtaccgcaa aggacgccga cagctgtgat gatgcggagc agcgtatctc ctcgatcatt cctgtgcttc gagagtcgct gtttctttca gcaaacggca ctcctgcggc aagctcgggg gagcggacct ttcgtgccta cattagccgg caaatcagtg tgtttctacc gcccatggca ccggagccca cgacgcggat acagcaacgt tgttgggggc ggaaaaactt ggagatcatg tttgacctgc tcggtgtggc gtcactgcag agaattgccg gagcgcctgt tgaggcagtg acaaagaatg agaaggcggc agtccttcgg caaatcattt acgaactagc gacggtcctt tcggagggtg acgcggaatt cgcagaaact tcttgcgcca aaataaacgg cacgtcctca gtcgatgcgg aggtgcaaac ccgtttgctt gcagagatgg cgctgcagcg cctcgcacat gagttgattc atcttcacac gatgttgctg atggatcagg cgtatgaaac ctatgtgaag ctgggatacc cgagcgaggg gatgctggag aagagtggct tctgctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat