TrcrB.19511.a

KREN3

CENTER ID: TrcrB.19511.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TrcrB.19511.a.B1.GE40810 full length 1 593
TrcrB.19511.a.B2.GE40811 RNase III-like catalytic-like domain 116 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TcCLB.506679.90
RefSeq: XP_820036.1
UniProt: Q4E095

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRACVITRPV AAARARGRIK CNYTDIFSWG SSPPEQDGGV SKGEMNRMKS RFYNSGQRRC TLCDEYLEAS FSTHCCHVSH IARVGILERA TAILQKETAE VMTSSAHEPA SKVAQMVRVL LETWWKRLNE VEQETLLDYK RIASLSSPSA RRRLWRVRFL LEFLRDREVI RDCLTLAKGT PSNEGSFVRT KRFERSEMIG DNIVKVVVPD RLVRLFPSTE GGVTYKLACI QQLLDSNEGL LQIYDYIDLN SIIGTRLPNN KTKSDVVESL FGELQTFLWA SEVACGLNQY PAIPNAEHRY VRALVEHLLN ELTHMVIMWR IESTLENAKE YLEEQIQLKL QSSQQCALRG GAGGCTVVKE VECDRGRYAV LPLLLSFPYP SASAESRQHR QRPSTPPPVR RVGENGTPQM LFTTSIPKPE LMVSHYRPRL RVVRSSQEYR LEAVKALTMQ RNLFTNILPA STSTLLGSRV SAKGEKQQLG HLSFPGLAEG KYPTWAREVR IAEVRRRVEE QMARRGVYVE TIDWGSMLAA LARTLTGFHA IRAPRLQSEP TKAPTKPLSP PTVAAAAEVF PPVEGKLKIV EEMCIPPLKP TVE
NT Sequence
atgcgtgcat gcgttatcac acggcccgta gctgcagcgc gagcacgagg gcggattaag tgcaattaca cggacatttt ttcatggggt tcgtcacctc ccgagcaaga cggaggtgtc tccaaggggg agatgaatcg gatgaagtcg cgcttctaca actcagggca gcggcgctgt acgttgtgtg acgaatattt ggaggcttct ttctctacac attgttgcca cgtcagccac attgcgcggg ttggaattct cgagcgggcc accgctatcc tgcagaagga gacggcagaa gttatgacga gctctgcaca tgaacccgca tcaaaggtgg cacaaatggt acgagtactt ctggaaactt ggtggaagag actgaacgag gtggagcagg agacgttact ggactacaaa cgtattgcct ccctcagctc accaagtgcc aggaggagac tctggcgggt ccgcttcttg ttggagtttt tgcgtgatcg cgaggtgatt cgtgactgcc tgacgttggc gaaagggacc ccctctaatg aaggctcttt tgttcgtacc aaacgttttg agcgcagcga gatgattgga gacaacatag tgaaggtcgt ggtgccggat cgtcttgtgc gtcttttccc atcgacagag ggcggtgtaa cgtacaagct tgcgtgtatc cagcagctcc ttgacagtaa tgagggttta ctgcagattt atgactacat tgacctgaat tccatcattg gtactcgatt accgaacaac aagacgaagt ccgacgtggt ggaatccctt tttggggaac ttcagacgtt tctttgggca agcgaagtgg cgtgtgggtt aaatcaatac cctgccattc caaacgccga gcaccgttat gtgcgggccc tcgtcgagca cctgctgaat gagctgaccc acatggtcat catgtggcgc attgaatcaa ccttggagaa cgcgaaggaa tacttggaag agcaaattca gctaaagttg cagtcatcgc agcagtgtgc tttgaggggg ggagcgggtg gatgcaccgt ggtgaaggaa gttgagtgtg accgtggtcg ctacgccgtt ctaccgcttt tgttgtcctt cccttatccg agtgcttcag cggaatcacg gcaacacaga cagcgaccaa gcacgccccc tccggtacgt cgtgtgggcg agaatggaac accccaaatg ttgtttacaa cctccatacc aaagcctgag ttgatggtgt cgcactacag gccgcgatta cgtgtggtgc gaagctcaca ggaataccga ttggaggcgg tgaaagcgct gacgatgcag cgcaacttat tcacgaatat tctcccagcg tcaacgtcta cgcttttggg aagtcgggtg agcgccaagg gcgaaaaaca gcagttaggc cacctctcat ttccaggatt agctgaagga aaatatccca cttgggctcg agaagtgcga atagcggagg tgcgccggcg tgtggaagaa caaatggcgc ggcggggagt ttatgtggag acaatagact ggggaagcat gctggctgcg ctggcgcgga cgttgacagg gtttcatgcc ataagagcgc cccggctgca aagtgaaccg acgaaggcac caacaaaacc tttatcgcca ccaactgtgg ccgcggcagc agaagtattt ccgccggttg aaggaaaatt aaagatcgtg gaagagatgt gtattccacc attgaagccc acggtggaat ag
Details for TrcrB.19511.a.B1.GE40810
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 68kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR ACVITRPVAA ARARGRIKCN YTDIFSWGSS PPEQDGGVSK GEMNRMKSRF YNSGQRRCTL CDEYLEASFS THCCHVSHIA RVGILERATA ILQKETAEVM TSSAHEPASK VAQMVRVLLE TWWKRLNEVE QETLLDYKRI ASLSSPSARR RLWRVRFLLE FLRDREVIRD CLTLAKGTPS NEGSFVRTKR FERSEMIGDN IVKVVVPDRL VRLFPSTEGG VTYKLACIQQ LLDSNEGLLQ IYDYIDLNSI IGTRLPNNKT KSDVVESLFG ELQTFLWASE VACGLNQYPA IPNAEHRYVR ALVEHLLNEL THMVIMWRIE STLENAKEYL EEQIQLKLQS SQQCALRGGA GGCTVVKEVE CDRGRYAVLP LLLSFPYPSA SAESRQHRQR PSTPPPVRRV GENGTPQMLF TTSIPKPELM VSHYRPRLRV VRSSQEYRLE AVKALTMQRN LFTNILPAST STLLGSRVSA KGEKQQLGHL SFPGLAEGKY PTWAREVRIA EVRRRVEEQM ARRGVYVETI DWGSMLAALA RTLTGFHAIR APRLQSEPTK APTKPLSPPT VAAAAEVFPP VEGKLKIVEE MCIPPLKPTV E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg catgcgttat cacacggccc gtagctgcag cgcgagcacg agggcggatt aagtgcaatt acacggacat tttttcatgg ggttcgtcac ctcccgagca agacggaggt gtctccaagg gggagatgaa tcggatgaag tcgcgcttct acaactcagg gcagcggcgc tgtacgttgt gtgacgaata tttggaggct tctttctcta cacattgttg ccacgtcagc cacattgcgc gggttggaat tctcgagcgg gccaccgcta tcctgcagaa ggagacggca gaagttatga cgagctctgc acatgaaccc gcatcaaagg tggcacaaat ggtacgagta cttctggaaa cttggtggaa gagactgaac gaggtggagc aggagacgtt actggactac aaacgtattg cctccctcag ctcaccaagt gccaggagga gactctggcg ggtccgcttc ttgttggagt ttttgcgtga tcgcgaggtg attcgtgact gcctgacgtt ggcgaaaggg accccctcta atgaaggctc ttttgttcgt accaaacgtt ttgagcgcag cgagatgatt ggagacaaca tagtgaaggt cgtggtgccg gatcgtcttg tgcgtctttt cccatcgaca gagggcggtg taacgtacaa gcttgcgtgt atccagcagc tccttgacag taatgagggt ttactgcaga tttatgacta cattgacctg aattccatca ttggtactcg attaccgaac aacaagacga agtccgacgt ggtggaatcc ctttttgggg aacttcagac gtttctttgg gcaagcgaag tggcgtgtgg gttaaatcaa taccctgcca ttccaaacgc cgagcaccgt tatgtgcggg ccctcgtcga gcacctgctg aatgagctga cccacatggt catcatgtgg cgcattgaat caaccttgga gaacgcgaag gaatacttgg aagagcaaat tcagctaaag ttgcagtcat cgcagcagtg tgctttgagg gggggagcgg gtggatgcac cgtggtgaag gaagttgagt gtgaccgtgg tcgctacgcc gttctaccgc ttttgttgtc cttcccttat ccgagtgctt cagcggaatc acggcaacac agacagcgac caagcacgcc ccctccggta cgtcgtgtgg gcgagaatgg aacaccccaa atgttgttta caacctccat accaaagcct gagttgatgg tgtcgcacta caggccgcga ttacgtgtgg tgcgaagctc acaggaatac cgattggagg cggtgaaagc gctgacgatg cagcgcaact tattcacgaa tattctccca gcgtcaacgt ctacgctttt gggaagtcgg gtgagcgcca agggcgaaaa acagcagtta ggccacctct catttccagg attagctgaa ggaaaatatc ccacttgggc tcgagaagtg cgaatagcgg aggtgcgccg gcgtgtggaa gaacaaatgg cgcggcgggg agtttatgtg gagacaatag actggggaag catgctggct gcgctggcgc ggacgttgac agggtttcat gccataagag cgccccggct gcaaagtgaa ccgacgaagg caccaacaaa acctttatcg ccaccaactg tggccgcggc agcagaagta tttccgccgg ttgaaggaaa attaaagatc gtggaagaga tgtgtattcc accattgaag cccacggtgg aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for TrcrB.19511.a.B2.GE40811
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV RVLLETWWKR LNEVEQETLL DYKRIASLSS PSARRRLWRV RFLLEFLRDR EVIRDCLTLA KGTPSNEGSF VRTKRFERSE MIGDNIVKVV VPDRLVRLFP STEGGVTYKL ACIQQLLDSN EGLLQIYDYI DLNSIIGTRL PNNKTKSDVV ESLFGELQTF LWASEVACGL NQYPAIPNAE HRYVRALVEH LLNELTHMVI MWRIESTLEN AKEYLEEQIQ LKLQSSQQCA LRGGAGGCTV VKEVECDRGR YAVLPLLLSF PYPSASAESR QHRQRPSTPP PVRRVGENGT PQMLFTTSIP KPEL
Validated NT Sequence
atcaactcag gctttggtat ggaggttgta aacaacattt ggggtgttcc attctcgccc acacgacgta ccggaggggg cgtgcttggt cgctgtctgt gttgccgtga ttccgctgaa gcactcggat aagggaagga caacaaaagc ggtagaacgg cgtagcgacc acggtcacac tcaacttcct tcaccacggt gcatccaccc gctccccccc tcaaagcaca ctgctgcgat gactgcaact ttagctgaat ttgctcttcc aagtattcct tcgcgttctc caaggttgat tcaatgcgcc acatgatgac catgtgggtc agctcattca gcaggtgctc gacgagggcc cgcacataac ggtgctcggc gtttggaatg gcagggtatt gatttaaccc acacgccact tcgcttgccc aaagaaacgt ctgaagttcc ccaaaaaggg attccaccac gtcggacttc gtcttgttgt tcggtaatcg agtaccaatg atggaattca ggtcaatgta gtcataaatc tgcagtaaac cctcattact gtcaaggagc tgctggatac acgcaagctt gtacgttaca ccgccctctg tcgatgggaa aagacgcaca agacgatccg gcaccacgac cttcactatg ttgtctccaa tcatctcgct gcgctcaaaa cgtttggtac gaacaaaaga gccttcatta gagggggtcc ctttcgccaa cgtcaggcag tcacgaatca cctcgcgatc acgcaaaaac tccaacaaga agcggacccg ccagagtctc ctcctggcac ttggtgagct gagggaggca atacgtttgt aatccagtaa cgtctcctgc tccacctcgt tcagtctctt ccaccaagtt tccagaagta ctcgtaccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMV RVLLETWWKR LNEVEQETLL DYKRIASLSS PSARRRLWRV RFLLEFLRDR EVIRDCLTLA KGTPSNEGSF VRTKRFERSE MIGDNIVKVV VPDRLVRLFP STEGGVTYKL ACIQQLLDSN EGLLQIYDYI DLNSIIGTRL PNNKTKSDVV ESLFGELQTF LWASEVACGL NQYPAIPNAE HRYVRALVEH LLNELTHMVI MWRIESTLEN AKEYLEEQIQ LKLQSSQQCA LRGGAGGCTV VKEVECDRGR YAVLPLLLSF PYPSASAESR QHRQRPSTPP PVRRVGENGT PQMLFTTSIP KPELMVSHY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtac gagtacttct ggaaacttgg tggaagagac tgaacgaggt ggagcaggag acgttactgg actacaaacg tattgcctcc ctcagctcac caagtgccag gaggagactc tggcgggtcc gcttcttgtt ggagtttttg cgtgatcgcg aggtgattcg tgactgcctg acgttggcga aagggacccc ctctaatgaa ggctcttttg ttcgtaccaa acgttttgag cgcagcgaga tgattggaga caacatagtg aaggtcgtgg tgccggatcg tcttgtgcgt cttttcccat cgacagaggg cggtgtaacg tacaagcttg cgtgtatcca gcagctcctt gacagtaatg agggtttact gcagatttat gactacattg acctgaattc catcattggt actcgattac cgaacaacaa gacgaagtcc gacgtggtgg aatccctttt tggggaactt cagacgtttc tttgggcaag cgaagtggcg tgtgggttaa atcaataccc tgccattcca aacgccgagc accgttatgt gcgggccctc gtcgagcacc tgctgaatga gctgacccac atggtcatca tgtggcgcat tgaatcaacc ttggagaacg cgaaggaata cttggaagag caaattcagc taaagttgca gtcatcgcag cagtgtgctt tgaggggggg agcgggtgga tgcaccgtgg tgaaggaagt tgagtgtgac cgtggtcgct acgccgttct accgcttttg ttgtccttcc cttatccgag tgcttcagcg gaatcacggc aacacagaca gcgaccaagc acgccccctc cggtacgtcg tgtgggcgag aatggaacac cccaaatgtt gtttacaacc tccataccaa agcctgagtt gatggtgtcg cactactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t