SSGCID: Target Page

TrcrB.19510.a

KREN2

CENTER ID:	TrcrB.19510.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19510.a.B1.GE40808	full length		1	532
TrcrB.19510.a.B2.GE40809	RNase III-like catalytic-like domain		69	373

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TcCLB.506679.210
RefSeq:	XP_820048.1
UniProt:	Q4E082

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKRGCCHVA LGAVWEVTSL LISRRSHAAT APQSGANFNP RRVRLVSSTS TLGAGAESLP VDVSPLSSDP NDPYRHVCIY RKDMVSDVGG RAFQLSVDVP LKKFVDLFSD FDAKRCKLCN ESYMQWHSHS GVIPHSGREA LMLEMVRAYC GTPEEIAKMW WHRLHTSPVF NRIPSLSHEN SHIRKRRLQY LLQFLKERDV IRDTFNVQQS AGGAGRSWEF ERLEWIGDNV VKYVFNDRFN VIFPVREGGI RGRLGYAQFM IDGNDGLARA YDHLELQKLT LSDRVVSKFK SDVVETLFGE LQLFLWSSET DVGTTYFELP FTPEMFSLRA LVWHVMEELA HVMFMYHVEH ILGAIQRIVR EHQLQFIRAD PSLRGHTDLQ NELASTLYAK KSLTGSGKLT PTKTSPSLRF KDGFAALYHE SSNYDGFKRV YALGGLLPRP FSREELSATP SFLPHLQEDG VMASRVPSDW WSKLMDTTEE DAAVRQGVVS TLSLSSLHAA VTPTPHPRSD AAVTPHLSVP ELKDNLLVAE LV

NT Sequence

atgctgaagc gcgggtgttg ccatgttgcc ctgggggccg tgtgggaagt cacttcactg ttaatttcaa ggcgaagtca tgctgcgaca gccccgcaat cgggtgcaaa ttttaatcca cgccgcgtac gacttgtttc ttctacgtca acactcggcg caggtgcgga gtcgttgccc gtggatgtga gccctttgtc gtccgatcca aacgatccct acaggcatgt ctgcatctac cgcaaggaca tggtatcaga cgttggtggt cgcgcatttc aacttagtgt ggatgtgccg ctgaaaaaat ttgtggacct ttttagcgat tttgatgcca aacggtgcaa actgtgcaac gaaagttaca tgcagtggca cagccatagc ggagttattc ctcattcagg tcgagaggcg ctgatgctgg agatggtgcg tgcatattgt ggtacacctg aagaaattgc aaagatgtgg tggcaccgcc ttcatacctc tccggttttt aaccgcattc cctccctgag ccatgagaac agtcatattc gtaaacgtcg gcttcagtac ttacttcaat ttttaaagga acgagatgtc attcgggaca cctttaatgt gcagcagagc gctggaggcg ctggtcgttc atgggaattt gaacgccttg agtggattgg ggataacgtg gtgaagtatg ttttcaatga tcgttttaat gtcatttttc cggtgcgcga gggtgggatc cgtggtcgac tgggctacgc tcaattcatg attgacggca atgacgggct ggcaagggca tatgatcatc ttgagctgca gaagttgaca ctgagcgatc gcgttgtaag caaattcaag agtgatgtgg tggagacgct ttttggggag ctgcagttat tcttgtggtc ttcagagaca gacgttggta ccacgtactt tgagttgccc tttacgccgg agatgttttc tcttagggca cttgtgtggc atgtgatgga agagctggcg cacgtcatgt tcatgtatca cgttgagcac attcttggtg cgattcaacg cattgtccgg gagcatcagc tgcagtttat ccgcgccgat ccgtcgctcc gcggtcacac ggaccttcag aatgagctgg cgagcaccct gtacgccaag aagagtttga caggatcagg aaaactgaca ccgacgaaga cgtcccctag tttgcgattc aaagacggct ttgccgcgtt gtaccatgaa tcatcgaatt acgacgggtt taaacgtgtg tacgcactcg gtggtctttt gccacgcccc ttttctcgcg aggagttgtc agcgacacca tcattcctgc cgcacctgca ggaggatgga gtgatggcca gccgggtgcc gtcagactgg tggtcaaagc tgatggacac gacggaggag gacgcggcgg tacgacaagg tgtggtttct actttatcgc tatcatcttt gcatgccgcc gtgacaccga caccacatcc gcgctcggac gctgcagtga caccacatct aagcgtgccg gaactcaaag acaacctgtt ggtggcggaa ctggtgtaa

Details for TrcrB.19510.a.B1.GE40808

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	61kDa
OBSERVED MW:	61kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	96
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML KRGCCHVALG AVWEVTSLLI SRRSHAATAP QSGANFNPRR VRLVSSTSTL GAGAESLPVD VNPLSSDPND PYRHVCIYRK DMVSDVGGRA FQLSVDVPLK KFVDLFSDFD AKRCKLCNES YMQWHSHSGV IPHSGREALM LEMVRAYCGT PEEIAKMWWH RLHTSPVFNR IPSLSHENSH IRKRRLQYLL QFLKERDVIR DTFNVQQXXG GAGRXWEFXX XXXIGXXXVK YVFNDRFNVI FPVREGGIRG RLGYAQFMID GNDGLARAYD HLELQKFTLS DRVVSKFKSD VVETLFGELQ LFLWSSETDV GTTYFELPFT PEMFSLRALV WHVMEELAHV MFMYHVEHIL GAIQRIVREH QLQFIRADPS LRGHTDLQNE LASTLYAKKS LTGSGKLTPT KTSPSLRFKD GFAALYHESS NYDGFKRVYA LGGLLPRPFS RDELSATPSF LPHLQEDGVM ASRVPSDWWS KLMDTTEEDA AVRQVVVSTL SPSSLHAAVT PTPHPRSDAA VTPHLSVPEL KD

Validated NT Sequence

gttgtctttg agttccggca cgcttagatg tggtgtcact gcagcgtccg agcgcggatg tggtgtcggt gtcacggcgg catgcaaaga tgatggtgat aaagtagaaa ccacaacttg tcgtaccgcc gcgtcctcct cagtcgtgtc catcagcttt gaccaccagt ctgacggcac ccgactggcc atcactccat cctcctgcag gtgcggcagg aatgatggtg tcgctgacaa ctcatcgcga gaaaaggggc gtggcaaaag accaccaagt gcgtacacac gtttgaaccc gtcgtaattc gatgactcat ggtacaacgc ggcaaagccg tctttgaatc gcaaactagg ggacgtcttc gtcggtgtca gttttcctga tcctgtcaaa ctcttcttgg cgtacagagt gctcgccagc tcattctgaa ggtccgtgtg gccgcggagc gacggatcgg cgcggatgaa ctgcagctga tgctcccgga caatgcgctg aatcgcacca agaatgtgct caacgtgata catgaacatg acgtgcgcca gctcttccat cacatgccac acaagtgccc taagagaaaa catctccggc gtaaagggca actcaaagta cgtggtacca acgtctgtct ctgaagacca caagaataac tgcagctccc caaaaagcgt ctccaccaca tcactcttga atttgcttac aacgcgatcg ctcagtgtga acttctgcag ctcaagatgg tcatatgccc ttgccagccc gtcattgccg tcaatcatga attgagcgta gcccagtcga ccacggatcc caccctcgcg caccggaaaa atgacattaa aacgatcatt gaaaacatac ttcaccannn ngnccccaat nnnntcannn nnnncaaatt cccannnncg accagcacct ccannncnct gctgcacatt aaaggtgtcc cgaatgacat ctcgttcctt taaaaattga agtaagtact gcagccgacg tttacgaata tgactgttct catggctcag ggagggaatg cggttaaaaa ccggagaggt atgaaggcgg tgccaccaca tctttgcaat ttcttcaggt gtcccacaat atgcacgcac catctccagc atcagcgcct cccgacctga atgaggaata actccactgt gactgtgcca ctgcatgtaa ctttcgttgc acagtttgca ccgtttggca tcaaaatcgc taaaaagatc cacaaatttt ttcagcggca catccacact aagttgaaat gcgcgaccac caacgtctga taccatgtcc ttgcggtaga tgcagacatg cctgtaggga tcgtttggat cggacgacaa agggttcaca tccacgggca acgactccgc acctgcgccg agtgttgacg tagaagaaac aagtcgtacg cggcgtggat taaaatttgc acccgattgc ggggctgtcg cggcatgact tcgccttgaa attaacagtg aagtgacttc ccacacggcc cccagggcaa catggcaaca cccgcgcttc agcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHML KRGCCHVALG AVWEVTSLLI SRRSHAATAP QSGANFNPRR VRLVSSTSTL GAGAESLPVD VSPLSSDPND PYRHVCIYRK DMVSDVGGRA FQLSVDVPLK KFVDLFSDFD AKRCKLCNES YMQWHSHSGV IPHSGREALM LEMVRAYCGT PEEIAKMWWH RLHTSPVFNR IPSLSHENSH IRKRRLQYLL QFLKERDVIR DTFNVQQSAG GAGRSWEFER LEWIGDNVVK YVFNDRFNVI FPVREGGIRG RLGYAQFMID GNDGLARAYD HLELQKLTLS DRVVSKFKSD VVETLFGELQ LFLWSSETDV GTTYFELPFT PEMFSLRALV WHVMEELAHV MFMYHVEHIL GAIQRIVREH QLQFIRADPS LRGHTDLQNE LASTLYAKKS LTGSGKLTPT KTSPSLRFKD GFAALYHESS NYDGFKRVYA LGGLLPRPFS REELSATPSF LPHLQEDGVM ASRVPSDWWS KLMDTTEEDA AVRQGVVSTL SLSSLHAAVT PTPHPRSDAA VTPHLSVPEL KDNLLVAELV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctga agcgcgggtg ttgccatgtt gccctggggg ccgtgtggga agtcacttca ctgttaattt caaggcgaag tcatgctgcg acagccccgc aatcgggtgc aaattttaat ccacgccgcg tacgacttgt ttcttctacg tcaacactcg gcgcaggtgc ggagtcgttg cccgtggatg tgagcccttt gtcgtccgat ccaaacgatc cctacaggca tgtctgcatc taccgcaagg acatggtatc agacgttggt ggtcgcgcat ttcaacttag tgtggatgtg ccgctgaaaa aatttgtgga cctttttagc gattttgatg ccaaacggtg caaactgtgc aacgaaagtt acatgcagtg gcacagccat agcggagtta ttcctcattc aggtcgagag gcgctgatgc tggagatggt gcgtgcatat tgtggtacac ctgaagaaat tgcaaagatg tggtggcacc gccttcatac ctctccggtt tttaaccgca ttccctccct gagccatgag aacagtcata ttcgtaaacg tcggcttcag tacttacttc aatttttaaa ggaacgagat gtcattcggg acacctttaa tgtgcagcag agcgctggag gcgctggtcg ttcatgggaa tttgaacgcc ttgagtggat tggggataac gtggtgaagt atgttttcaa tgatcgtttt aatgtcattt ttccggtgcg cgagggtggg atccgtggtc gactgggcta cgctcaattc atgattgacg gcaatgacgg gctggcaagg gcatatgatc atcttgagct gcagaagttg acactgagcg atcgcgttgt aagcaaattc aagagtgatg tggtggagac gctttttggg gagctgcagt tattcttgtg gtcttcagag acagacgttg gtaccacgta ctttgagttg ccctttacgc cggagatgtt ttctcttagg gcacttgtgt ggcatgtgat ggaagagctg gcgcacgtca tgttcatgta tcacgttgag cacattcttg gtgcgattca acgcattgtc cgggagcatc agctgcagtt tatccgcgcc gatccgtcgc tccgcggtca cacggacctt cagaatgagc tggcgagcac cctgtacgcc aagaagagtt tgacaggatc aggaaaactg acaccgacga agacgtcccc tagtttgcga ttcaaagacg gctttgccgc gttgtaccat gaatcatcga attacgacgg gtttaaacgt gtgtacgcac tcggtggtct tttgccacgc cccttttctc gcgaggagtt gtcagcgaca ccatcattcc tgccgcacct gcaggaggat ggagtgatgg ccagccgggt gccgtcagac tggtggtcaa agctgatgga cacgacggag gaggacgcgg cggtacgaca aggtgtggtt tctactttat cgctatcatc tttgcatgcc gccgtgacac cgacaccaca tccgcgctcg gacgctgcag tgacaccaca tctaagcgtg ccggaactca aagacaacct gttggtggcg gaactggtgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for TrcrB.19510.a.B2.GE40809

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMD PNDPYRHVCI YRKDMVSDVG GRAFQLSVDV PLKKFVDLFS DFDAKRCKLC NESYMQWHSH SGVIPHSGRE ALMLEMVRAY CGTPEEIAKM WWHRLHTSPV FNRIPSLSHE NSHIRKRRLQ YLLQFLKERD VIRDTFNVQQ SAGGAGRSWE FERLEWIGDN VVKYVFNDRF NVIFPVREGG IRGRLGYAQF MIDGNDGLAR AYDHLELQKL TLSDRVVSKF KSDVVETLFG ELQLFLWSSE TDVGTTYFEL PFTPEMFSLR ALVWHVMEEL AHVMFMYHVE HILGAIQRIV REHQLQFI

Validated NT Sequence

ggataaactg cagctgatgc tcccggacaa tgcgttgaat cgcaccaaga atgtgctcaa cgtgatacat gaacatgacg tgcgccagct cttccatcac atgccacaca agtgccctaa gagaaaacat ctccggcgta aagggcaact caaagtacgt ggtaccaacg tctgtctctg aagaccacaa gaataactgc agctccccaa aaagcgtctc caccacatca ctcttgaatt tgcttacaac gcgatcgctc agtgtcaact tctgcagctc aagatgatca tatgcccttg ccagcccgtc attgccgtca atcatgaatt gagcgtagcc cagtcgacca cggatcccac cctcgcgcac cggaaaaatg acattaaaac gatcattgaa aacatacttc accacgttat ccccaatcca ctcaaggcgt tcaaattccc atgaacgacc agcgcctcca gcgctctgct gcacattaaa ggtgtcccga atgacatctc gttcctttaa aaattgaagt aagtactgaa gccgacgttt acgaatatga ctgttctcat ggctcaggga gggaatgcgg ttaaaaaccg gagaggtatg aaggcggtgc caccacatct ttgcaatttc ttcaggtgta ccacaatatg cacgcaccat ctccagcatc agcgcctctc gacctgaatg aggaataact ccgctatggc tgtgccactg catgtaactt tcgttgcaca gtttgcaccg tttggcatca aaatcgctaa aaaggtccac aaattttttc agcggcacat ccacactaag ttgaaatgcg cgaccaccaa cgtctgatac catgtccttg cggtagatgc agacatgcct gtagggatcg tttggatcca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHDP NDPYRHVCIY RKDMVSDVGG RAFQLSVDVP LKKFVDLFSD FDAKRCKLCN ESYMQWHSHS GVIPHSGREA LMLEMVRAYC GTPEEIAKMW WHRLHTSPVF NRIPSLSHEN SHIRKRRLQY LLQFLKERDV IRDTFNVQQS AGGAGRSWEF ERLEWIGDNV VKYVFNDRFN VIFPVREGGI RGRLGYAQFM IDGNDGLARA YDHLELQKLT LSDRVVSKFK SDVVETLFGE LQLFLWSSET DVGTTYFELP FTPEMFSLRA LVWHVMEELA HVMFMYHVEH ILGAIQRIVR EHQLQFIRAD PSL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatccaa acgatcccta caggcatgtc tgcatctacc gcaaggacat ggtatcagac gttggtggtc gcgcatttca acttagtgtg gatgtgccgc tgaaaaaatt tgtggacctt tttagcgatt ttgatgccaa acggtgcaaa ctgtgcaacg aaagttacat gcagtggcac agccatagcg gagttattcc tcattcaggt cgagaggcgc tgatgctgga gatggtgcgt gcatattgtg gtacacctga agaaattgca aagatgtggt ggcaccgcct tcatacctct ccggttttta accgcattcc ctccctgagc catgagaaca gtcatattcg taaacgtcgg cttcagtact tacttcaatt tttaaaggaa cgagatgtca ttcgggacac ctttaatgtg cagcagagcg ctggaggcgc tggtcgttca tgggaatttg aacgccttga gtggattggg gataacgtgg tgaagtatgt tttcaatgat cgttttaatg tcatttttcc ggtgcgcgag ggtgggatcc gtggtcgact gggctacgct caattcatga ttgacggcaa tgacgggctg gcaagggcat atgatcatct tgagctgcag aagttgacac tgagcgatcg cgttgtaagc aaattcaaga gtgatgtggt ggagacgctt tttggggagc tgcagttatt cttgtggtct tcagagacag acgttggtac cacgtacttt gagttgccct ttacgccgga gatgttttct cttagggcac ttgtgtggca tgtgatggaa gagctggcgc acgtcatgtt catgtatcac gttgagcaca ttcttggtgc gattcaacgc attgtccggg agcatcagct gcagtttatc cgcgccgatc cgtcgctctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat