SSGCID: Target Page

TrcrB.19509.a

KREPA2

CENTER ID:	TrcrB.19509.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19509.a.B1.GE40806	full length		1	590
TrcrB.19509.a.B2.GE40807	C2H2 zinc finger and OB fold		391	590

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TcCLB.506247.30
RefSeq:	XP_820317.1
UniProt:	Q4E153

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRLLLAGAL AKFSRRVGAR WLACAPKARY AVNRLNDGSY FFLPALNCQE RFQSSSDKKF HCSVCKKAFR LEMAAKLHLQ QAHGGDGSVE AGPGPGAETA PTAIPSAPPS SPIERNFTDE EERRPRRMRP TPKPLHQPDR GVPDADMAEL LGVWDKIGLN RLEGSFVHST MVMKVFAARP DVTEQPLYEP VTPEGDNPFE KMDQIDARAA PESYVKDHAL TEVDLKGPFA PAPDQTLNPF RAGKVDNPFA KVPTVHREVP RILQPQQQVP KEEPVTAPIT PFGQLPMFGK QQQTKETIPA KPLQQNISVP GEVASPFTAA VANSPFIGQV ASPFTSTPDA AATPFEAPPF TVASPFAAPG MQASSPFTAT DALSPFAQNA SCQTSAFPMA MGFSNLTPNV QTGQRAHVEE KCPTCGKSFS TFDGLAMHSK AKHGVLLERE AKKDGKQRGR SVPDLPAYIP SPVDLSSSSP FGTKTAVAAS WVETELIPHA QCVSNITIVG RVLDVTQAME GVSHVTVFVQ GEEVGEEETV TVHCSGDINI QVRDSVKRNV TIFLTGTLRL HPVYEASNNK YYVSPVVHVA SPTGTLAVIA

NT Sequence

atgcgtcggt tgttgctcgc tggggcgctg gcgaagttct cccgcagagt gggcgcaaga tggctagcgt gtgctccaaa ggcacgttac gccgtcaacc gtctcaacga tggcagctac ttttttttac cagcgctaaa ttgccaggag cgatttcaat cttcctcgga caaaaagttc cactgtagcg tttgcaaaaa agcctttcgc ctggagatgg cggcgaagtt acacctccaa caggcacacg ggggtgatgg ctcggtggag gccggcccag gtcccggcgc tgaaaccgcc cccacggcta ttccctcagc gccgccttcg agccctattg agaggaattt tacggatgaa gaggaacgcc gtcctcgtcg tatgaggccc acaccaaagc cgcttcatca acccgaccgc ggggtgccag atgctgacat ggcagagctt ctcggtgtgt gggacaaaat tggtcttaac aggctggaag ggagttttgt tcacagcaca atggtgatga aggtgtttgc tgcacgccct gatgttacag agcagcctct ctatgagcct gtcactcctg aaggggataa ccccttcgag aaaatggatc aaattgatgc aagggcggcg ccagagtcct acgtcaaaga ccacgcgttg acagaggtag acctgaaggg tccatttgcc cctgcacctg atcaaacatt aaatcctttt cgtgcaggga aggtggacaa tccgttcgca aaagtcccaa cagtgcatcg cgaggtgccg aggattctcc agccgcaaca acaggtgcca aaggaagagc ctgtgacggc gccaattaca ccatttggac aattgccaat gtttggcaaa cagcagcaaa caaaggagac cattccagca aagccattac agcagaacat ctctgttcct ggagaagttg cctccccctt cacagcggcg gtggctaatt caccatttat tggtcaggtg gcgtcaccct ttacttctac acctgacgcc gccgctacgc cttttgaagc accaccgttt acagtagcat caccctttgc tgcacctgga atgcaggcaa gttcgccatt tactgcaacc gatgcgctta gtccctttgc tcaaaatgcc tcatgccaga ccagcgcatt tcctatggcc atgggttttt caaatttgac gccaaatgta caaactgggc agagggctca tgttgaggaa aaatgtccta cttgcgggaa aagtttttcc accttcgatg gacttgccat gcacagcaaa gccaaacatg gcgttcttct tgagcgggaa gcgaagaagg atggcaagca aaggggacgc agtgtccctg atttacccgc ctatatacca agtcctgttg atctttcatc gtcatcaccg tttggaacaa agaccgccgt tgctgcatca tgggtagaaa ctgagcttat accccatgct caatgtgtga gcaacattac aattgttggt cgtgtgctgg atgtcacaca ggcgatggaa ggtgtatccc atgtcactgt gtttgttcaa ggcgaagagg ttggggagga agaaactgta actgttcact gttctggcga cattaacatt caggttcggg atagcgtgaa gcgtaatgtt acaatatttt tgactggaac actgcgactt catcccgttt acgaggcaag caacaacaag tattacgtga gccctgttgt gcatgttgca tcgccaacag ggacactagc cgtgatcgca taa

Details for TrcrB.19509.a.B1.GE40806

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	65kDa
OBSERVED MW:	65kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	95
PERCENT COVERAGE:	40

Validated AA Sequence

Validated NT Sequence

ttggcgatgc aacatgcaca acagggctca cgtaatactt gttgttgctt gcctcgtaaa cgggatgaag tcgcagtgtt ccagtcaaaa atattgtaac attacgcttc acgctatccc gaacctgaat gttaatgtcg ccagaacagt gaacagttac agtttcttcc tccccaacct cttcgccttg aacaaacaca gtgacatggg atacaccttc catcgcctgt gtgacatcca gcacacgacc aacaattgta atgttgctca cacattgagc atggggtata agctcagttt ctacccatga tgcagcaacg gcggtctttg ttccaaacgg tgatgacgat gaaagatcaa caggacttgg tatataggcg ggtaaatcag ggacactgcg tcccctttgc ttgccatcct tcttcgcttc ccgctcaaga agaacgccat gtttggcttt gctgtgcatg gcaagtccat cgaaggtgga aaaacttttc ccgcaagtag gacatttttc ctcaacatga gccctctgcc cagtttgtac atttggcgtc aaatttgaaa aacccatggc cataggaaat gcgctggtct ggcatgaggc attttgagca aagggactaa gcgcatcggt tgcagtaaat ggcgaacttg cctgcattcc aggtgcagca aagggtgatg ctactgtaaa cggtggtgct tcaaaaggcg tagcggcggc gtcaggtgta gaagtaaagg gtgacgccac ctgaccaata aatggtgaat tagccaccgc cgctgtnnnn nnngaggcaa cttctccagg aacagagatg ttctgctgta atggcttngc tggaatgnnn nnnnnnnnnn nnntgctgtt tgccaaacat tngcaattgn ncnnnnnnnn tnnnnnncnn nnnnnnnnnn nnnnnnnttt ggcnnntnnn nnnnnnnnnn nnnnnnnnnn nnnannnnnn nnnncactgn tggnnnnnnn nnnnnnnnnn ngtnnnnnnn nnctgcacnn nnnnnattta atgttngatc aggtgcaggg gcnnnnnnnn nnnncagggt ctacctctgt caacgcgtnn nctttgnnnt aggactctgg cgccgccctt gcatcaattt gatccatttt ctcgaagggg ttatcccctt caggagtgac aggctcatag agaggctgct ctgtaacatc agggcgtgca gcaaacacct tcatcaccat tgtgctgtga acaaaactcc cttccagcct gttaagacca attttgtccc acacaccgag aagctctgcc atgtcagcat ctggcacccc gcggtcgggt tgatgaagcg gctttggtgt gggcctcata cgacgaggac ggcgttcctc ttcatccgta aaattcctct caatagggct cgaaggcggc gctgagggaa tagccgtggg ggcggtttca gcgccgggac ctgggccggc ctccaccgag ccatcacccc cgtgtgcctg ttggaggtgt aacttcgccg ccatctccag gcgaaaggct tttttgcaaa cgctacagtg gaactttttg tccgaggaag attgaaatcg ctcctggcaa tttagcgctg gtaaaaaaaa gtagctgcca tcgttgagac ggttgacggc gtaacgtgcc tttggagcac acgctagcca tcttgcgccc actctgcggg agaacttcgc cagcgcccca gcgagcaaca accgacgcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHMR RLLLAGALAK FSRRVGARWL ACAPKARYAV NRLNDGSYFF LPALNCQERF QSSSDKKFHC SVCKKAFRLE MAAKLHLQQA HGGDGSVEAG PGPGAETAPT AIPSAPPSSP IERNFTDEEE RRPRRMRPTP KPLHQPDRGV PDADMAELLG VWDKIGLNRL EGSFVHSTMV MKVFAARPDV TEQPLYEPVT PEGDNPFEKM DQIDARAAPE SYVKDHALTE VDLKGPFAPA PDQTLNPFRA GKVDNPFAKV PTVHREVPRI LQPQQQVPKE EPVTAPITPF GQLPMFGKQQ QTKETIPAKP LQQNISVPGE VASPFTAAVA NSPFIGQVAS PFTSTPDAAA TPFEAPPFTV ASPFAAPGMQ ASSPFTATDA LSPFAQNASC QTSAFPMAMG FSNLTPNVQT GQRAHVEEKC PTCGKSFSTF DGLAMHSKAK HGVLLEREAK KDGKQRGRSV PDLPAYIPSP VDLSSSSPFG TKTAVAASWV ETELIPHAQC VSNITIVGRV LDVTQAMEGV SHVTVFVQGE EVGEEETVTV HCSGDINIQV RDSVKRNVTI FLTGTLRLHP VYEASNNKYY VSPVVHVASP TGTLAVIA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtc ggttgttgct cgctggggcg ctggcgaagt tctcccgcag agtgggcgca agatggctag cgtgtgctcc aaaggcacgt tacgccgtca accgtctcaa cgatggcagc tacttttttt taccagcgct aaattgccag gagcgatttc aatcttcctc ggacaaaaag ttccactgta gcgtttgcaa aaaagccttt cgcctggaga tggcggcgaa gttacacctc caacaggcac acgggggtga tggctcggtg gaggccggcc caggtcccgg cgctgaaacc gcccccacgg ctattccctc agcgccgcct tcgagcccta ttgagaggaa ttttacggat gaagaggaac gccgtcctcg tcgtatgagg cccacaccaa agccgcttca tcaacccgac cgcggggtgc cagatgctga catggcagag cttctcggtg tgtgggacaa aattggtctt aacaggctgg aagggagttt tgttcacagc acaatggtga tgaaggtgtt tgctgcacgc cctgatgtta cagagcagcc tctctatgag cctgtcactc ctgaagggga taaccccttc gagaaaatgg atcaaattga tgcaagggcg gcgccagagt cctacgtcaa agaccacgcg ttgacagagg tagacctgaa gggtccattt gcccctgcac ctgatcaaac attaaatcct tttcgtgcag ggaaggtgga caatccgttc gcaaaagtcc caacagtgca tcgcgaggtg ccgaggattc tccagccgca acaacaggtg ccaaaggaag agcctgtgac ggcgccaatt acaccatttg gacaattgcc aatgtttggc aaacagcagc aaacaaagga gaccattcca gcaaagccat tacagcagaa catctctgtt cctggagaag ttgcctcccc cttcacagcg gcggtggcta attcaccatt tattggtcag gtggcgtcac cctttacttc tacacctgac gccgccgcta cgccttttga agcaccaccg tttacagtag catcaccctt tgctgcacct ggaatgcagg caagttcgcc atttactgca accgatgcgc ttagtccctt tgctcaaaat gcctcatgcc agaccagcgc atttcctatg gccatgggtt tttcaaattt gacgccaaat gtacaaactg ggcagagggc tcatgttgag gaaaaatgtc ctacttgcgg gaaaagtttt tccaccttcg atggacttgc catgcacagc aaagccaaac atggcgttct tcttgagcgg gaagcgaaga aggatggcaa gcaaagggga cgcagtgtcc ctgatttacc cgcctatata ccaagtcctg ttgatctttc atcgtcatca ccgtttggaa caaagaccgc cgttgctgca tcatgggtag aaactgagct tataccccat gctcaatgtg tgagcaacat tacaattgtt ggtcgtgtgc tggatgtcac acaggcgatg gaaggtgtat cccatgtcac tgtgtttgtt caaggcgaag aggttgggga ggaagaaact gtaactgttc actgttctgg cgacattaac attcaggttc gggatagcgt gaagcgtaat gttacaatat ttttgactgg aacactgcga cttcatcccg tttacgaggc aagcaacaac aagtattacg tgagccctgt tgtgcatgtt gcatcgccaa cagggacact agccgtgatc gcatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for TrcrB.19509.a.B2.GE40807

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG FSNLTPNVQT GQRAHVEEKC PTCGKSFSTF DGLAMHSKAK HGVLLEREAK KDGKQRGRSV PDLPAYIPSP VDLSSSSPFG TKTAVAASWV ETELIPHAQC VSNITIVGRV LDVTQAMEGV SHVTVFVQGE EVGEEETVTV HCSGDINIQV RDSVKRNVTI FLTGTLRLHP VYEASNNKYY VSPVVHVASP TGTLAVIA

Validated NT Sequence

atcttactca cttatgcgat cacggctagt gtccctgttg gcgatgcaac atgcacaaca gggctcacgt aatacttgtt gttgcttgcc tcgtaaacgg gatgaagtcg cagtgttcca gtcaaaaata ttgtaacatt acgcttcacg ctatcccgaa cctgaatgtt aatgtcgcca gaacagtgaa cagttacagt ttcttcctcc ccaacctctt cgccttgaac aaacacagtg acatgggata caccttccat cgcctgtgtg acatccagca cacgaccaac aattgtaatg ttgctcacac attgagcatg gggtataagc tcagtttcta cccatgatgc agcaacggcg gtctttgttc caaacggtga tgacgatgaa agatcaacag gacttggtat ataggcgggt aaatcaggga cactgcgtcc cctttgcttg ccatccttct tcgcttcccg ctcaagaaga acgccatgtt tggctttgct gtgcatggca agtccatcga aggtggaaaa acttttcccg caagtaggac atttttcctc aacatgagcc ctctgcccag tttgtacatt tggcgtcaaa tttgaaaaac ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtt tttcaaattt gacgccaaat gtacaaactg ggcagagggc tcatgttgag gaaaaatgtc ctacttgcgg gaaaagtttt tccaccttcg atggacttgc catgcacagc aaagccaaac atggcgttct tcttgagcgg gaagcgaaga aggatggcaa gcaaagggga cgcagtgtcc ctgatttacc cgcctatata ccaagtcctg ttgatctttc atcgtcatca ccgtttggaa caaagaccgc cgttgctgca tcatgggtag aaactgagct tataccccat gctcaatgtg tgagcaacat tacaattgtt ggtcgtgtgc tggatgtcac acaggcgatg gaaggtgtat cccatgtcac tgtgtttgtt caaggcgaag aggttgggga ggaagaaact gtaactgttc actgttctgg cgacattaac attcaggttc gggatagcgt gaagcgtaat gttacaatat ttttgactgg aacactgcga cttcatcccg tttacgaggc aagcaacaac aagtattacg tgagccctgt tgtgcatgtt gcatcgccaa cagggacact agccgtgatc gcatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat