SSGCID: Target Page

TrcrB.19508.a

KREPB4

CENTER ID:	TrcrB.19508.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19508.a.B1.GE40805	full length		1	400

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TcCLB.507773.30
RefSeq:	XP_807543.1
UniProt:	Q4CZJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRVSAHGVS VHFAVVLTTA MRGATCLLCD VEYDSWGKHV AGASHIARNA ICRTYVAPER HDAMMQQLWK HIRLDFGYID EVTHKKEDRR RGRLASTIRH LHEQGILRHS IPKISVDGDG VRSVVVTKDA FVNLMMVGES YAKQETVDRV ARLMPRLEAV ELSSVVQYIL SKRRLARLFD ELDMRRTLLN DSATGDAVEG PSENRKNGES DIARECDDAA EETLRLRQEE KTVIIFSCLG ELQMYSRQDR SHSVATRAAA EQLVLNVLGT HVMENIIAEL VHTVLQTVVE EGTPVWRAYC DDLTNKMFEG TRATPPSLDL SPAPATGAAN FGEIRSTDLL SLYTLQVGEN AASVLPTAGR RSWSDVTRAL ALELTVPNPV NKSALFAAAA PRLSSKKKPA

NT Sequence

atgcgacgcg tctccgcgca tggagtgagc gttcacttcg ctgtggtgct tacgacagcg atgcgaggag ccacttgtct tctgtgcgac gtggagtatg actcgtgggg gaagcacgtt gccggtgctt cgcatattgc ccgcaacgct atttgcagaa cctacgttgc tcctgagcgg cacgatgcga tgatgcagca gctgtggaaa cacatccgcc tggactttgg gtatattgac gaggtgacac acaaaaagga agatcgtcgc cgtggccggc tggcatctac gatacgtcat ctgcatgagc aggggatatt gcgccactcc atcccaaaga tttctgtgga cggcgacggg gttaggtcgg ttgttgtcac gaaggatgcc tttgtcaacc tcatgatggt gggcgaaagc tacgctaagc aagagaccgt ggatcgtgtc gcgcgtctta tgccacgcct ggaggcggtg gagctaagca gcgtcgtaca gtacattcta tcgaaacgac gtcttgcccg cctttttgac gaacttgata tgcggcggac gcttttaaac gactctgcga cgggtgatgc cgtggagggg ccgagtgaga atcgaaaaaa tggggagagt gacatcgcac gggaatgtga cgatgccgcg gaggagacgc tgcgtctccg ccaagaggaa aaaactgtta ttattttcag ctgccttggc gaattgcaga tgtactcacg gcaggatcga tcacacagcg tggccacacg ggcggcggca gagcagctcg tgttgaatgt gctcggcaca cacgtgatgg agaacattat cgctgagttg gtgcacacag tgctgcagac tgtagtggag gaggggacgc ctgtgtggcg agcgtactgc gacgatttga caaacaaaat gtttgagggc accagggcga cgccgccatc gcttgatttg tcaccagcac cagcgacagg tgcggcaaac tttggtgaaa ttcgctcgac tgatcttctt tccttataca cgcttcaggt gggagaaaat gctgcgtcgg ttctgccgac tgcgggacga cgttcttgga gtgatgtgac gcgtgccttg gctctggaac tcactgtgcc caacccagtc aacaagagtg ctttgtttgc cgcagcggca ccacggctgt cgtcgaagaa gaaaccggcc tag

Details for TrcrB.19508.a.B1.GE40805

HARVESTED ON:	1/31/2017
SEQUENCED ON:	11/22/2016
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR RVSAHGVSVH FAVVLTTAMR GATCLLCDVE YDSWGKHVAG ASHIARNAIC RTYVAPERHD AMMQQLWKHI RLDFGYIDEV THKKEDRRRG RLASTIRHLH EQGILRHSIP KISVDGDGVR SVVVTKDAFV NLMMVGESYA KQETVDRVAR LMPRLEAVEL SSVVQYILSK RRLARLFDEL DMRRTLLNDS ATGDAVEGPS ENRKNGESDI ARECDDAAEE TLRLRQEEKT VIIFSCLGEL QMYSRQDRSH SVATRAAAEQ LVLNVLGTHV MENIIAELVH TVLQTVVEEG TPVWRAYCDD LTNKMFEGTR ATPPSLDLSP APATGAANFG EIRSTDLLSL YTLQVGENAA SVLPTAGRRS WSDVTRALAL ELTVPNPVNK SALFAAAAPR

Validated NT Sequence

agccgtggtg ccgctgcggc aaacaaagca ctcttgttga ctgggttggg cacagtgagt tccagagcca aggcacgcgt cacatcactc caagaacgtc gtcccgcagt cggcagaacc gacgcagcat tttctcccac ctgaagcgtg tataaggaaa gaagatcagt cgagcgaatt tcaccaaagt ttgccgcacc tgtcgctggt gctggtgaca aatcaagcga tggcggcgtc gccctggtgc cctcaaacat tttgtttgtc aaatcgtcgc agtacgctcg ccacacaggc gtcccctcct ccactacagt ctgcagcact gtgtgcacca actcagcgat aatgttctcc atcacgtgtg tgccgagcac attcaacacg agctgctctg ccgccgcccg tgtggccacg ctgtgtgatc gatcctgccg tgagtacatc tgcaattcgc caaggcagct gaaaataata acagtttttt cctcttggcg gagacgcagc gtctcctccg cggcatcgtc acattcccgt gcgatgtcac tctccccatt ttttcgattc tcactcggcc cctccacggc atcacccgtc gcagagtcgt ttaaaagcgt ccgccgcata tcaagttcgt caaaaaggcg ggcaagacgt cgtttcgata gaatgtactg tacgacgctg cttagctcca ccgcctccag gcgtggcata agacgcgcga cacgatccac ggtctcttgc ttagcgtagc tttcgcccac catcatgagg ttgacaaagg catccttcgt gacaacaacc gacctaaccc cgtcgccgtc cacagaaatc tttgggatgg agtggcgcaa tatcccctgc tcatgcagat gacgtatcgt agatgccagc cggccacggc gacgatcttc ctttttgtgt gtcacctcgt caatataccc aaagtccagg cggatgtgtt tccacagctg ctgcatcatc gcatcgtgcc gctcaggagc aacgtaggtt ctgcaaatag cgttgcgggc aatatgcgaa gcaccggcaa cgtgcttccc ccacgagtca tactccacgt cgcacagaag acaagtggct cctcgcatcg ctgtcgtaag caccacagcg aagtgaacgc tcactccatg cgcggagacg cgtcgcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgac gcgtctccgc gcatggagtg agcgttcact tcgctgtggt gcttacgaca gcgatgcgag gagccacttg tcttctgtgc gacgtggagt atgactcgtg ggggaagcac gttgccggtg cttcgcatat tgcccgcaac gctatttgca gaacctacgt tgctcctgag cggcacgatg cgatgatgca gcagctgtgg aaacacatcc gcctggactt tgggtatatt gacgaggtga cacacaaaaa ggaagatcgt cgccgtggcc ggctggcatc tacgatacgt catctgcatg agcaggggat attgcgccac tccatcccaa agatttctgt ggacggcgac ggggttaggt cggttgttgt cacgaaggat gcctttgtca acctcatgat ggtgggcgaa agctacgcta agcaagagac cgtggatcgt gtcgcgcgtc ttatgccacg cctggaggcg gtggagctaa gcagcgtcgt acagtacatt ctatcgaaac gacgtcttgc ccgccttttt gacgaacttg atatgcggcg gacgctttta aacgactctg cgacgggtga tgccgtggag gggccgagtg agaatcgaaa aaatggggag agtgacatcg cacgggaatg tgacgatgcc gcggaggaga cgctgcgtct ccgccaagag gaaaaaactg ttattatttt cagctgcctt ggcgaattgc agatgtactc acggcaggat cgatcacaca gcgtggccac acgggcggcg gcagagcagc tcgtgttgaa tgtgctcggc acacacgtga tggagaacat tatcgctgag ttggtgcaca cagtgctgca gactgtagtg gaggagggga cgcctgtgtg gcgagcgtac tgcgacgatt tgacaaacaa aatgtttgag ggcaccaggg cgacgccgcc atcgcttgat ttgtcaccag caccagcgac aggtgcggca aactttggtg aaattcgctc gactgatctt ctttccttat acacgcttca ggtgggagaa aatgctgcgt cggttctgcc gactgcggga cgacgttctt ggagtgatgt gacgcgtgcc ttggctctgg aactcactgt gcccaaccca gtcaacaaga gtgctttgtt tgccgcagcg gcaccacggc tgtcgtcgaa gaagaaaccg gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat