TrcrB.19507.a

KREPB5

CENTER ID: TrcrB.19507.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.19507.a.B1.GE40804 full length 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TcCLB.506925.390
RefSeq: XP_821585.1
UniProt: Q4E4L1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRGVLAFCV AAPAAARCAL TGRRVEDWEA HNRTFEMRVF ELLKNRLASP TEPLPANQVY VTIANPCRLI DEFNFAKTTR RMDRLRISLG FLRRHNVLLH GLLFYVKETK TWNTTAGFGR LALFGESLLR HEVRARTLRL FPSIDSATYA SLTASILNED ALCAIFDRLM MKSLVGAKPE GKDRDWSLTK EQRSSMLCAI VGEMSWFSSR TKATDRTHNN ALFPPSDALI LHVLCCHVLE SLPAELIYTV VEPRVHRLKE VWVNEPMSIP SQMHLKPRTI NALSLSLVKK PISEEERLRK VAAQAATVCF PSLSSEPVIG SFISKMKPRW NYKRFDERRY QILGDDKRCF LPLFSSPTPR DSGACVVVTL TDERRRELVS PAFKPGEE
NT Sequence
atgaaacgtg gcgtcctggc gttctgtgtc gccgccccgg cagctgcccg gtgtgcactt accggcagga gagtggagga ctgggaggcg cacaaccgta cctttgagat gcgagttttt gagctgctca agaaccgtct ggcctctccg acggagccgc tgccggcgaa tcaagtatac gtaaccatag cgaacccatg tcgtctcatt gacgagttta attttgctaa aactacacga aggatggatc gtctgcggat ttctcttggt tttttgcggc gccacaatgt gcttcttcat ggtttgttgt tttacgtgaa ggaaacaaaa acttggaaca ccacggcggg gtttgggcga ctggctctat tcggcgagag tcttcttcgt catgaggttc gtgcccgtac gcttcggctg tttccatcca tagattccgc gacttacgcc tcactgaccg cctccatact gaatgaagat gcattatgcg ccattttcga ccggttgatg atgaagtctc ttgttggagc taagcctgag ggtaaggacc gcgactggtc tttaacaaaa gagcagcgca gtagcatgtt gtgtgccatt gtaggtgaaa tgagttggtt ctcttctcga acaaaggcca cggataggac acataacaat gcacttttcc caccatctga tgcactcatt cttcatgtgt tgtgttgcca cgtattggag tctctgccgg cggagcttat ttacaccgtt gtggaaccac gtgttcatcg cctcaaggag gtttgggtca atgaaccaat gtctattcca tcacagatgc atttaaaacc cagaacaatt aacgccttgt ctttgtcttt ggtgaaaaag cctatttctg aagaggaacg tcttcggaag gtggcagctc aggccgccac tgtgtgtttc ccttctcttt cttccgaacc tgttatcggg agctttatat caaaaatgaa gccgcgttgg aactataaac gttttgatga acgacgatat caaatactgg gagatgacaa gcgctgcttt ttgcctctgt tttcttcgcc aacgcccagg gattctggtg catgtgtagt cgtgacgctg acggatgaaa gacggaggga gctagtttcc ccagcgttta agcctggcga agagtag
Details for TrcrB.19507.a.B1.GE40804
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 45kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMK RGVLAFCVAA PAAARCALTG RRVEDWEAHN RTFEMRVFEL LKNRLASPTE PLPANQVYVT IANPCRLIDE FNFAKTTRRM DRLRISLGFL RRHNVLLHGL LFYVKETKTW NTTAGFGRLA LFGESLLRHE VRARTLRLFP SIDSATYASL TASILNEDAL CAIFDRLMMK SLVGAKPEGK DRDWSLTKEQ RSSMLCAIVG EMSWFSSRTK ATDRTHNNAL FPPSDALILH VLCCHVLESL PAELIYTVVE PRVHRLKEVW VNEPMSIPSQ MHLKPRTINA LSLSLVKKPI SEEERLRKVA AQAATVCFPS LSSERVVGSF ISKMKPRWNY KRFDERRYQI LGDDKRCFLP LFSSPTPRDS GACVVVTLTD ERRRELVS
Validated NT Sequence
ggagaaacta actccctccg tctttcatcc gtcagcgtca cgactacaca tgcaccagaa tccctgggcg ttggcgaaga aaacagaggc aaaaagcagc gcttgtcatc tcccagtatt tgatatcgtc gttcatcaaa acgtttatag ttccaacgcg gtttcatttt tgatataaag ctcccgacaa cacgttcgga agaaagggaa gggaaacaca cagtggcggc ctgagctgcc accttccgaa gacgttcctc ttcagaaata ggctttttca ccaaagacaa agacaaggcg ttaattgttc tgggttttaa atgcatctgt gatggaatag acattggttc attgacccaa acctccttga ggcgatgaac acgtggttcc acaacggtgt aaataagctc cgccggcaga gactccaata cgtggcaaca caacacatga agaatgagtg catcagatgg tgggaaaagt gcattgttat gtgtcctatc cgtggccttt gttcgagaag agaaccaact catttcacct acaatggcac acaacatgct actgcgctgc tcttttgtta aagaccagtc gcggtcctta ccctcaggct tagctccaac aagagacttc atcatcaacc ggtcgaaaat ggcgcataat gcatcttcat tcagtatgga ggcggtcagt gaggcgtaag tcgcggaatc tatggatgga aacagccgaa gcgtacgggc acgaacctca tgacgaagaa gactctcgcc gaatagagcc agtcgcccaa accccgccgt ggtgttccaa gtttttgttt ccttcacgta aaacaacaaa ccatgaagaa gcacattgtg gcgccgcaaa aaaccaagag aaatccgcag acgatccatc cttcgtgtag ttttagcaaa attaaactcg tcaatgagac gacatgggtt cgctatggtt acgtatactt gattcgccgg cagcggctcc gtcggagagg ccagacggtt cttgagcagc tcaaaaactc gcatctcaaa ggtacggttg tgcgcctccc agtcctccac tctcctgccg gtaagtgcac accgggcagc tgccggggcg gcgacacaga acgccaggac gccacgtttc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMK RGVLAFCVAA PAAARCALTG RRVEDWEAHN RTFEMRVFEL LKNRLASPTE PLPANQVYVT IANPCRLIDE FNFAKTTRRM DRLRISLGFL RRHNVLLHGL LFYVKETKTW NTTAGFGRLA LFGESLLRHE VRARTLRLFP SIDSATYASL TASILNEDAL CAIFDRLMMK SLVGAKPEGK DRDWSLTKEQ RSSMLCAIVG EMSWFSSRTK ATDRTHNNAL FPPSDALILH VLCCHVLESL PAELIYTVVE PRVHRLKEVW VNEPMSIPSQ MHLKPRTINA LSLSLVKKPI SEEERLRKVA AQAATVCFPS LSSEPVIGSF ISKMKPRWNY KRFDERRYQI LGDDKRCFLP LFSSPTPRDS GACVVVTLTD ERRRELVSPA FKPGEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac gtggcgtcct ggcgttctgt gtcgccgccc cggcagctgc ccggtgtgca cttaccggca ggagagtgga ggactgggag gcgcacaacc gtacctttga gatgcgagtt tttgagctgc tcaagaaccg tctggcctct ccgacggagc cgctgccggc gaatcaagta tacgtaacca tagcgaaccc atgtcgtctc attgacgagt ttaattttgc taaaactaca cgaaggatgg atcgtctgcg gatttctctt ggttttttgc ggcgccacaa tgtgcttctt catggtttgt tgttttacgt gaaggaaaca aaaacttgga acaccacggc ggggtttggg cgactggctc tattcggcga gagtcttctt cgtcatgagg ttcgtgcccg tacgcttcgg ctgtttccat ccatagattc cgcgacttac gcctcactga ccgcctccat actgaatgaa gatgcattat gcgccatttt cgaccggttg atgatgaagt ctcttgttgg agctaagcct gagggtaagg accgcgactg gtctttaaca aaagagcagc gcagtagcat gttgtgtgcc attgtaggtg aaatgagttg gttctcttct cgaacaaagg ccacggatag gacacataac aatgcacttt tcccaccatc tgatgcactc attcttcatg tgttgtgttg ccacgtattg gagtctctgc cggcggagct tatttacacc gttgtggaac cacgtgttca tcgcctcaag gaggtttggg tcaatgaacc aatgtctatt ccatcacaga tgcatttaaa acccagaaca attaacgcct tgtctttgtc tttggtgaaa aagcctattt ctgaagagga acgtcttcgg aaggtggcag ctcaggccgc cactgtgtgt ttcccttctc tttcttccga acctgttatc gggagcttta tatcaaaaat gaagccgcgt tggaactata aacgttttga tgaacgacga tatcaaatac tgggagatga caagcgctgc tttttgcctc tgttttcttc gccaacgccc agggattctg gtgcatgtgt agtcgtgacg ctgacggatg aaagacggag ggagctagtt tccccagcgt ttaagcctgg cgaagagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at