TrcrB.19503.a

KREPB7

CENTER ID: TrcrB.19503.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.19503.a.B1.GE40801 full length 1 414
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TcCLB.506445.80
RefSeq: XP_813817.1
UniProt: Q4DHG3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHRRTLRRLQ QVVQNPPVGS ANIPSPYATE GGHVPIWSIV NGFVQKRNWV FRNPEWCDLC KEPVALWVNH HGRKDHALMD MHYTQMVEFP RRWNPEEILA VFLEELGLSV EAYQKYYGGY EREHRNELYA MLVALEEAKM LHFGEPRDTY LYRMQGGLRG MDHQGALVLH RYILGPFMRI YPNGHIQDYS NLVDFVTCAY NMETVYDLCG FYTLDKVALK ADYKPSSPAA LGLGGIAVHS SATHGFAQQA MEKPQQSPQP QPQRHMAVGT GGRSAADMEE EAFSRKAVFV RQLLGQLRWL TLPDEEHPAG HMFPQHLIFL GEICLKSLVA EIVAARLCEY MVRVEPVWRD HGYERVKLDV DKIMRKENDI LPEPVKFFYR PMSPKMDDLY STNRGNLDEA LEKAVVAPLS GKGA
NT Sequence
atgcatcgga gaacactgcg ccgactgcaa caagtcgtac aaaacccacc cgttggttct gccaacattc cttcgcccta cgcaacggaa gggggccacg ttcccatctg gtcgatagtt aacggattcg tgcagaaacg caactgggtg tttcgcaacc cggagtggtg tgatctctgc aaggagccgg tggccctctg ggtgaatcac cacgggcgaa aagaccacgc tttgatggat atgcactaca cgcagatggt ggagtttcca cgccgatgga atccagagga aattctagcc gtcttcttgg aagaattggg tctctctgta gaagcttacc agaagtacta cggcgggtat gagcgggaac accgcaatga gctttatgcg atgcttgtgg cattagagga ggcaaagatg cttcactttg gagagccacg agatacatac ttgtaccgca tgcaaggtgg cctccgtggg atggatcacc agggggcttt ggtgctgcac cgatacatcc ttggcccctt catgcgcata taccccaatg ggcatattca agactattca aacctggtgg actttgtaac ttgtgcgtac aacatggaga ccgtctatga tttatgcggc ttttacacac tggataaagt agcgttgaag gcggattaca agccaagctc ccctgctgca ttaggcctgg gaggcattgc ggtacattct tccgccacac atggcttcgc tcaacaagcg atggaaaaac cacaacaatc accacaacca caaccacagc gacacatggc agtagggact ggtggccgta gcgctgccga tatggaagag gaagcattct caaggaaggc tgtctttgtt cggcaacttt tgggtcagtt acgctggttg acactgccag acgaagagca ccctgcgggt cacatgtttc cacagcactt gatttttctt ggtgaaatct gcctgaagtc gctcgtggct gaaattgtgg cggcgcgatt gtgtgagtac atggtgcgtg tggagcctgt ctggcgggat cacgggtacg aacgtgtgaa gcttgacgtg gacaaaatca tgagaaaaga aaacgatatt ttaccggagc ccgtaaagtt tttctaccgc cccatgtcgc ccaagatgga tgatctctac agcaccaacc ggggcaactt ggatgaggcg ctggagaagg ctgtggtcgc accattatcg ggtaagggtg cgtag
Details for TrcrB.19503.a.B1.GE40801
HARVESTED ON: data unavailable
SEQUENCED ON: 11/22/2016
EXPECTED MW: 48kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMH RRTLRRLQQV VQNPPVGSAN IPSPYATEGG HVPIWSIVNG FVQKRNWVFR NPEWCDLCKE PVALWVNHHG RKDHALMDMH YTQMVEFPRR WNPEEILAVF LEELGLSVEA YQKYYGGYER EHRNELYAML VALEEAKMLH FGEPRDTYLY RMQGGLRGMD HQGALVLHRY ILGPFMRIYP NGHIQDYSNL VDFVTCAYNM ETVYDLCGFY TLDKVALKAD YKPSSPAALG LGGIAVHSSA THGFAQQAME KPQQSPQPQP QRHMTVGTGG RSAADMEEEA FSRKAVFVRQ LLGQLRWLTL PDEEHPAGHM FPQHLMFLGE ICLKSLVAEI VAARLCEYMV RVEPVWRDHG YERVKLDVDK IMRKENDILP EPVKFFYRPM SPKMDDLYST NRGNLDEALE KAVV
Validated NT Sequence
tgcgaccaca gccttctcca gcgcttcatc caagttaccc cggttggtgc tgtagagatc atccatcttc ggcgacatgg ggcggtagaa aaactttacg ggctccggta gaatatcgtt ttctttcctc atgattttgt ccacgtcaag cttcacacgt tcgtatccgt gatcccgcca gacaggctcc acacgcacca tgtactcaca caatcgcgcc gccacaattt cagccacgag cgacttcagg cagatttcac caagaaacat caaatgctgt ggaaacatgt gaccggcagg gtgctcttcg tctggcagtg tcaaccagcg taactggccc aaaagttgcc gaacaaagac agccttcctt gagaatgcct cctcttccat atcggcagcg ctacggccac cggtccctac tgtcatgtgt cgctgtggtt gtggttgtgg tgattgttgt ggtttttcca tcgcttgttg agcgaagcca tgtgtggcag aagaatgtac cgcaatgcct cccaggccta atgcagcagg ggagcttggc ttgtaatccg ccttcaacgc tactttatcc agtgtataaa agccgcataa atcatagacg gtctccatgt tgtacgcaca agtcacaaag tccaccaggt ttgaatagtc ttgaatatgc ccattggggt atatgcgcat gaaggggcca aggatgtatc gatgcagcac caaagccccc tggtgatcca tcccacggag gccaccttgc atgcggtaca agtatgtatc tcgtggctct ccaaagtgaa gcatctttgc ctcctctaat gccacaagca tcgcgtaaag ctcattgcgg tgttcccgct catacccgcc gtagtacttc tggtaagctt ctaccgagag ccccaattct tccaagaaga cggctagaat ttcctctgga ttccatctgc gtggaaactc caccatctgc gtgtagtgca tatccatcaa agcgtggtct tttcgcccgt ggtgattcac ccagagggcc accggctcct tgcagagatc acaccactcc gggttgcgaa acacccagtt gcgtttctgc acgaatccgt taactatcga ccagatggga acgtggcccc cttccgttgc gtagggcgaa ggaatgttgg cagaaccaac aggtgggttt tgcacgacct gttgcagtcg gcgcagtgtt ctccgatgca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMH RRTLRRLQQV VQNPPVGSAN IPSPYATEGG HVPIWSIVNG FVQKRNWVFR NPEWCDLCKE PVALWVNHHG RKDHALMDMH YTQMVEFPRR WNPEEILAVF LEELGLSVEA YQKYYGGYER EHRNELYAML VALEEAKMLH FGEPRDTYLY RMQGGLRGMD HQGALVLHRY ILGPFMRIYP NGHIQDYSNL VDFVTCAYNM ETVYDLCGFY TLDKVALKAD YKPSSPAALG LGGIAVHSSA THGFAQQAME KPQQSPQPQP QRHMAVGTGG RSAADMEEEA FSRKAVFVRQ LLGQLRWLTL PDEEHPAGHM FPQHLIFLGE ICLKSLVAEI VAARLCEYMV RVEPVWRDHG YERVKLDVDK IMRKENDILP EPVKFFYRPM SPKMDDLYST NRGNLDEALE KAVVAPLSGK GA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatc ggagaacact gcgccgactg caacaagtcg tacaaaaccc acccgttggt tctgccaaca ttccttcgcc ctacgcaacg gaagggggcc acgttcccat ctggtcgata gttaacggat tcgtgcagaa acgcaactgg gtgtttcgca acccggagtg gtgtgatctc tgcaaggagc cggtggccct ctgggtgaat caccacgggc gaaaagacca cgctttgatg gatatgcact acacgcagat ggtggagttt ccacgccgat ggaatccaga ggaaattcta gccgtcttct tggaagaatt gggtctctct gtagaagctt accagaagta ctacggcggg tatgagcggg aacaccgcaa tgagctttat gcgatgcttg tggcattaga ggaggcaaag atgcttcact ttggagagcc acgagataca tacttgtacc gcatgcaagg tggcctccgt gggatggatc accagggggc tttggtgctg caccgataca tccttggccc cttcatgcgc atatacccca atgggcatat tcaagactat tcaaacctgg tggactttgt aacttgtgcg tacaacatgg agaccgtcta tgatttatgc ggcttttaca cactggataa agtagcgttg aaggcggatt acaagccaag ctcccctgct gcattaggcc tgggaggcat tgcggtacat tcttccgcca cacatggctt cgctcaacaa gcgatggaaa aaccacaaca atcaccacaa ccacaaccac agcgacacat ggcagtaggg actggtggcc gtagcgctgc cgatatggaa gaggaagcat tctcaaggaa ggctgtcttt gttcggcaac ttttgggtca gttacgctgg ttgacactgc cagacgaaga gcaccctgcg ggtcacatgt ttccacagca cttgattttt cttggtgaaa tctgcctgaa gtcgctcgtg gctgaaattg tggcggcgcg attgtgtgag tacatggtgc gtgtggagcc tgtctggcgg gatcacgggt acgaacgtgt gaagcttgac gtggacaaaa tcatgagaaa agaaaacgat attttaccgg agcccgtaaa gtttttctac cgccccatgt cgcccaagat ggatgatctc tacagcacca accggggcaa cttggatgag gcgctggaga aggctgtggt cgcaccatta tcgggtaagg gtgcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat