SSGCID: Target Page

TrcrB.19502.a

KREPA1

CENTER ID:	TrcrB.19502.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19502.a.B1.GE40799	full length		1	806
TrcrB.19502.a.B2.GE40800	C2H2 zinc finger and OB fold		530	806

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19502.a.B2.PS38172	C2H2 zinc finger and OB fold		530	806

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TcCLB.508741.270
RefSeq:	XP_821058.1
UniProt:	Q4E345

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKRLTHRGRC RPAGILTCDA WRNWRRVSRA SMAMAHSSHA FMILHNRPRS LRWVSTSSQT AGETAASCIP SSSAAGDDNS AVHADACGAC EAFAPGEARE LHFHEDFLFE EVDVHFLLLE EAHKKHGVLL NVPPQMSPSG TPPEVPEVMF PAVQLERLRG MQLAYEPTHL PPPLHTTGTR KLIIDDASPM ATLVTKTSIA STPTTTKSAT STKGGGGTAI LVRHLPPDPT MRFHCTACGK AFRLEFSAEQ HVKLKHPSDS KAAVGTGPGE GEIIEEAVAT AVTPTSPART AKETTSKATG AEKGGPSASE NGAAGGAGAT ESAKTQQPSK DGGLSGAEVS TAKMPYDKAT LALPSEELVD ELIKEIWDDI AARRDDIPKQ NDPNAYVPFN AVVEGTADRR KEIEANAKPT ARATPEGAAP GIKKPSMLSS GSMSAARAQK PGMSVPIKEL VKKYPNPFGD SPNAVLQELE KEPINPFIPQ EELEAQLQVA RETPPLASTP NGVANGIPLG SKTKTSSATA SAAFVTGTDS GRVQDKLKDR LRASRPSLTR SASLRRFVCP LCAEKKRKKA EAQDSNAIPS FRLLDALFDH VESAHEGEEL SEEQLHSLYK TQRSSALHAP QPSGNSGTGE TSDGAVDPNT HAFKDDDTIG MREETIQDAV RAASLPEMLQ KAAPPAAVEQ KALNVHVRAG SNTVLLGRIA DVQHGFIGTM AVTQYVLEID GENATSNKGD EGAKLSPESE KELIVIRCMG DNFPAALLKE QVRLGSTVLV QGTLRMNRHI DSASKRLHAY PFVQVVPPLG CVKVIG

NT Sequence

atgaagcgct tgactcatcg cggtcgctgc cgtcctgcag gcattttgac ctgcgatgca tggaggaact ggcgtagagt gtcgcgtgca tccatggcga tggcgcattc ctcacacgca ttcatgatat tacataaccg cccacgctcg ctacggtggg tgagcacatc ttcacagacc gcgggggaga ccgccgcctc ttgcatcccg tcttcttccg ccgccgggga tgacaacagc gccgtgcatg cggacgcttg tggtgcttgt gaggcctttg ccccaggtga ggcgagagag cttcatttcc atgaggactt tctttttgag gaggtggacg tgcacttctt gctgctggag gaggcgcaca agaaacacgg tgttctgctt aatgtgccgc cgcagatgag tccaagcgga acgccacccg aggtaccgga ggtgatgttt ccggctgtgc aactggagcg actgcgtggg atgcagttgg catacgagcc cacccatctc cctccaccgc tccacaccac aggcaccaga aaactcatta ttgacgacgc ctccccgatg gcgacacttg tgacaaagac ttccatagcg tctacgccta cgaccacgaa aagcgcaact tcgacgaaag gcgggggcgg aaccgccatt ttggtgcggc atttaccgcc ggacccaacg atgcggtttc actgcactgc ctgcgggaag gcgtttcgtc tggagttctc tgcagagcaa catgtgaagt tgaaacatcc atcggattca aaggcggcgg tggggacggg ccctggagag ggtgaaatta tcgaggaagc cgtggcgaca gctgtgaccc ccacatcccc tgcacgaacg gcgaaggaaa caacgtcaaa agcgactggg gcggaaaagg gaggaccgag cgccagtgaa aatggagccg caggaggagc aggggcgacg gagtctgcca aaacgcagca accaagtaag gacggcggat tgtctggtgc ggaggtcagc actgcaaaga tgccgtacga caaggcgaca ttggctttac cgagtgagga gctcgtggat gagctcatta aggaaatttg ggacgacatt gctgcacgtc gtgacgacat ccccaaacaa aatgatccca atgcatatgt tccttttaac gccgttgtgg aaggcaccgc agaccgacga aaggaaatag aggcaaatgc gaagcccacg gcccgcgcca cgccggaggg tgccgccccg gggataaaga aaccaagtat gctctctagt ggctctatga gtgctgcaag ggcgcagaaa ccaggtatga gcgtgcctat aaaagaactt gtgaaaaagt acccgaaccc ctttggcgac tctcccaatg cggtactgca ggaacttgaa aaggagccca tcaatccgtt cattccacag gaggagcttg aggcacagtt gcaggtggca cgggaaacgc cacctttggc gtccacacca aacggcgtgg cgaatggcat tcctcttggc agtaaaacaa agacttctag cgccacggct tctgctgctt ttgttaccgg taccgattcc ggtagggtgc aggataaatt gaaggatcga cttcgggcgt cacggccgag tttaacgcgg tcggcatccc tgcgtcgctt tgtgtgtccc ctttgtgcgg agaagaaaag aaagaaggcg gaggcacaag attccaatgc gattccttcc tttcgactac tggacgcact ctttgaccac gtggagtcgg cgcacgaggg ggaggagctg tcggaggaac agcttcacag tttatacaaa acgcagcgga gctcggcgtt acatgctccc cagccaagtg gcaacagtgg aacgggagaa accagcgatg gcgctgtcga cccgaatacc catgcgttca aggacgatga cacgattggc atgcgtgagg aaacaataca ggatgcagtg agggcggcgt cgttgccaga gatgttgcag aaggctgccc cacccgcagc agtggagcag aaggcactta atgtccacgt acgggctggg agtaacactg tgctgctggg acgtatcgcg gatgttcaac atggtttcat tggcacaatg gcggtgacgc agtatgtgtt ggaaattgac ggagagaatg ccacatccaa caaaggggac gagggtgcaa aattgagtcc agagagcgag aaggaactga tagtcatccg ttgcatgggt gataacttcc ctgccgcatt gctaaaggag caggtgcggc tgggcagcac ggtgcttgta cagggcacac ttcgcatgaa tcgacacatt gatagcgcat caaagcgact tcatgcgtat ccgtttgttc aggtggtgcc accacttggt tgtgtgaagg ttatcgggta a

Details for TrcrB.19502.a.B1.GE40799

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	87kDa
OBSERVED MW:	87kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK RLTHRGRCRP AGILTCDAWR NWRRVSRASM AMAHSSHAFM ILHNRPRSLR WVSTSSQTAG ETAASCIPSS SAAGDDNSAV HADACGACEA FAPGEARELH FHEDFLFEEV DVHFLLLEEA HKKHGVLLNV PPQMSPSGTP PEVPEVMFPA VQLERLRGMQ LAYEPTHLPP PLHTTGTRKL IIDDASPMAT LVTKTSIAST PTTTKSATST KGGGGTAILV RHLPPDPTMR FHCTACGKAF RLEFSAEQHV KLKHPSDSKA AVGTGPGEGE IIEEAVATAV TPTSPARTAK ETTSKATGAE KGGPSASENG AAGGAGATES AKTQQPSKDG GLSGAEVSTA KMPYDKATLA LPSEELVDEL IKEIWDDIAA RRDDIPKQND PNAYVPFNAV VEGTADRRKE IEANAKPTAR ATPEGAAPGI KKPSMLSSGS MSAARAQKPG MSVPIKELVK KYPNPFGDSP NAVLQELEKE PINPFIPQEE LEAQLQVARE TPPLASTPNG VANGIPLGSK TKTSSATASA AFVTGTDSGR VQDKLKDRLR ASRPSLTRSA SLRRFVCPLC AEKKRKKAEA QDSNAIPSFR LLDALFDHVE SAHEGEELSE EQLHSLYKTQ RSSALHAPQP SGNSGTGETS DGAVDPNTHA FKDDDTIGMR EETIQDAVRA ASLPEMLQKA APPAAVEQKA LNVHVRAGSN TVLLGRIADV QHGFIGTMAV TQYVLEIDGE NATSNKGDEG AKLSPESEKE LIVIRCMGDN FPAALLKEQV RLGSTVLVQG TLRMNRHIDS ASKRLHAYPF VQVVPPLGCV KVIG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc gcttgactca tcgcggtcgc tgccgtcctg caggcatttt gacctgcgat gcatggagga actggcgtag agtgtcgcgt gcatccatgg cgatggcgca ttcctcacac gcattcatga tattacataa ccgcccacgc tcgctacggt gggtgagcac atcttcacag accgcggggg agaccgccgc ctcttgcatc ccgtcttctt ccgccgccgg ggatgacaac agcgccgtgc atgcggacgc ttgtggtgct tgtgaggcct ttgccccagg tgaggcgaga gagcttcatt tccatgagga ctttcttttt gaggaggtgg acgtgcactt cttgctgctg gaggaggcgc acaagaaaca cggtgttctg cttaatgtgc cgccgcagat gagtccaagc ggaacgccac ccgaggtacc ggaggtgatg tttccggctg tgcaactgga gcgactgcgt gggatgcagt tggcatacga gcccacccat ctccctccac cgctccacac cacaggcacc agaaaactca ttattgacga cgcctccccg atggcgacac ttgtgacaaa gacttccata gcgtctacgc ctacgaccac gaaaagcgca acttcgacga aaggcggggg cggaaccgcc attttggtgc ggcatttacc gccggaccca acgatgcggt ttcactgcac tgcctgcggg aaggcgtttc gtctggagtt ctctgcagag caacatgtga agttgaaaca tccatcggat tcaaaggcgg cggtggggac gggccctgga gagggtgaaa ttatcgagga agccgtggcg acagctgtga cccccacatc ccctgcacga acggcgaagg aaacaacgtc aaaagcgact ggggcggaaa agggaggacc gagcgccagt gaaaatggag ccgcaggagg agcaggggcg acggagtctg ccaaaacgca gcaaccaagt aaggacggcg gattgtctgg tgcggaggtc agcactgcaa agatgccgta cgacaaggcg acattggctt taccgagtga ggagctcgtg gatgagctca ttaaggaaat ttgggacgac attgctgcac gtcgtgacga catccccaaa caaaatgatc ccaatgcata tgttcctttt aacgccgttg tggaaggcac cgcagaccga cgaaaggaaa tagaggcaaa tgcgaagccc acggcccgcg ccacgccgga gggtgccgcc ccggggataa agaaaccaag tatgctctct agtggctcta tgagtgctgc aagggcgcag aaaccaggta tgagcgtgcc tataaaagaa cttgtgaaaa agtacccgaa cccctttggc gactctccca atgcggtact gcaggaactt gaaaaggagc ccatcaatcc gttcattcca caggaggagc ttgaggcaca gttgcaggtg gcacgggaaa cgccaccttt ggcgtccaca ccaaacggcg tggcgaatgg cattcctctt ggcagtaaaa caaagacttc tagcgccacg gcttctgctg cttttgttac cggtaccgat tccggtaggg tgcaggataa attgaaggat cgacttcggg cgtcacggcc gagtttaacg cggtcggcat ccctgcgtcg ctttgtgtgt cccctttgtg cggagaagaa aagaaagaag gcggaggcac aagattccaa tgcgattcct tcctttcgac tactggacgc actctttgac cacgtggagt cggcgcacga gggggaggag ctgtcggagg aacagcttca cagtttatac aaaacgcagc ggagctcggc gttacatgct ccccagccaa gtggcaacag tggaacggga gaaaccagcg atggcgctgt cgacccgaat acccatgcgt tcaaggacga tgacacgatt ggcatgcgtg aggaaacaat acaggatgca gtgagggcgg cgtcgttgcc agagatgttg cagaaggctg ccccacccgc agcagtggag cagaaggcac ttaatgtcca cgtacgggct gggagtaaca ctgtgctgct gggacgtatc gcggatgttc aacatggttt cattggcaca atggcggtga cgcagtatgt gttggaaatt gacggagaga atgccacatc caacaaaggg gacgagggtg caaaattgag tccagagagc gagaaggaac tgatagtcat ccgttgcatg ggtgataact tccctgccgc attgctaaag gagcaggtgc ggctgggcag cacggtgctt gtacagggca cacttcgcat gaatcgacac attgatagcg catcaaagcg acttcatgcg tatccgtttg ttcaggtggt gccaccactt ggttgtgtga aggttatcgg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for TrcrB.19502.a.B2.GE40800

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	31kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS GRVQDKLKDR LRASRPSLTR SASLRRFVCP LCAEKKRKKA EAQDSNAIPS FRLLDALFDH VESAHEGEEL SEEQLHSLYK TQRSSALHAP QPSGNSGTGE TSDGAVDPNT HAFKDDDTIG MREETIQDAV RAASLPEMLQ KAAPPAAVEQ KALNVHVRAG SNTVLLGRIA DVQHGFIGTM AVTQYVLEID GENATSNKGD EGAKLSPESE KELIVIRCMG DNFPAALLKE QVRLGSTVLV QGTLRMNRHI DSASKRLHAY PFVQVVPPLG CVKVIG

Validated NT Sequence

atcttactct tacccgataa ccttcacaca accaagtggt ggcaccacct gaacaaacgg atacgcatga agtcgctttg atgcgctatc aatgtgtcga ttcatgcgaa gtgtgccctg tacaagcacc gtgctgccca gccgcacctg ctcctttagc aatgcggcag ggaagttatc acccatgcaa cggatgacta tcagttcctt ctcgctctct ggactcaatt ttgcaccctc gtcccctttg ttggatgtgg cattctctcc gtcaatttcc aacacatact gcgtcaccgc cattgtgcca atgaaaccat gttgaacatc cgcgatacgt cccagcagca cagtgttact cccagcccgt acgtggacat taagtgcctt ctgctccact gctgcgggtg gggcagcctt ctgcaacatc tctggcaacg acgccgccct cactgcatcc tgtattgttt cctcacgcat gccaatcgtg tcatcgtcct tgaacgcatg ggtattcggg tcgacagcgc catcgctggt ttctcccgtt ccactgttgc cacttggctg gggagcatgt aacgccgagc tccgctgcgt tttgtataaa ctgtgaagct gttcctccga cagctcctcc ccctcgtgcg ccgactccac gtggtcaaag agtgcgtcca gtagtcgaaa ggaaggaatc gcattggaat cttgtgcctc cgccttcttt cttttcttct ccgcacaaag gggacacaca aagcgacgca gggatgccga ccgcgttaaa ctcggccgtg acgcccgaag tcgatccttc aatttatcct gcaccctacc ggacatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHSG RVQDKLKDRL RASRPSLTRS ASLRRFVCPL CAEKKRKKAE AQDSNAIPSF RLLDALFDHV ESAHEGEELS EEQLHSLYKT QRSSALHAPQ PSGNSGTGET SDGAVDPNTH AFKDDDTIGM REETIQDAVR AASLPEMLQK AAPPAAVEQK ALNVHVRAGS NTVLLGRIAD VQHGFIGTMA VTQYVLEIDG ENATSNKGDE GAKLSPESEK ELIVIRCMGD NFPAALLKEQ VRLGSTVLVQ GTLRMNRHID SASKRLHAYP FVQVVPPLGC VKVIG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccggta gggtgcagga taaattgaag gatcgacttc gggcgtcacg gccgagttta acgcggtcgg catccctgcg tcgctttgtg tgtccccttt gtgcggagaa gaaaagaaag aaggcggagg cacaagattc caatgcgatt ccttcctttc gactactgga cgcactcttt gaccacgtgg agtcggcgca cgagggggag gagctgtcgg aggaacagct tcacagttta tacaaaacgc agcggagctc ggcgttacat gctccccagc caagtggcaa cagtggaacg ggagaaacca gcgatggcgc tgtcgacccg aatacccatg cgttcaagga cgatgacacg attggcatgc gtgaggaaac aatacaggat gcagtgaggg cggcgtcgtt gccagagatg ttgcagaagg ctgccccacc cgcagcagtg gagcagaagg cacttaatgt ccacgtacgg gctgggagta acactgtgct gctgggacgt atcgcggatg ttcaacatgg tttcattggc acaatggcgg tgacgcagta tgtgttggaa attgacggag agaatgccac atccaacaaa ggggacgagg gtgcaaaatt gagtccagag agcgagaagg aactgatagt catccgttgc atgggtgata acttccctgc cgcattgcta aaggagcagg tgcggctggg cagcacggtg cttgtacagg gcacacttcg catgaatcga cacattgata gcgcatcaaa gcgacttcat gcgtatccgt ttgttcaggt ggtgccacca cttggttgtg tgaaggttat cgggtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for TrcrB.19502.a.B2.PS38172

PURIFICATION DATe:	1/18/2017
CONCENTRATION:	2.2mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	18
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCCGGTA GGGTGCAGGA TAAATTGAAG GATCGACTTC GGGCGTCACG GCCGAGTTTA ACGCGGTCGG CATCCCTGCG TCGCTTTGTG TGTCCCCTTT GTGCGGAGAA GAAAAGAAAG AAGGCGGAGG CACAAGATTC CAATGCGATT CCTTCCTTTC GACTACTGGA CGCACTCTTT GACCACGTGG AGTCGGCGCA CGAGGGGGAG GAGCTGTCGG AGGAACAGCT TCACAGTTTA TACAAAACGC AGCGGAGCTC GGCGTTACAT GCTCCCCAGC CAAGTGGCAA CAGTGGAACG GGAGAAACCA GCGATGGCGC TGTCGACCCG AATACCCATG CGTTCAAGGA CGATGACACG ATTGGCATGC GTGAGGAAAC AATACAGGAT GCAGTGAGGG CGGCGTCGTT GCCAGAGATG TTGCAGAAGG CTGCCCCACC CGCAGCAGTG GAGCAGAAGG CACTTAATGT CCACGTACGG GCTGGGAGTA ACACTGTGCT GCTGGGACGT ATCGCGGATG TTCAACATGG TTTCATTGGC ACAATGGCGG TGACGCAGTA TGTGTTGGAA ATTGACGGAG AGAATGCCAC ATCCAACAAA GGGGACGAGG GTGCAAAATT GAGTCCAGAG AGCGAGAAGG AACTGATAGT CATCCGTTGC ATGGGTGATA ACTTCCCTGC CGCATTGCTA AAGGAGCAGG TGCGGCTGGG CAGCACGGTG CTTGTACAGG GCACACTTCG CATGAATCGA CACATTGATA GCGCATCAAA GCGACTTCAT GCGTATCCGT TTGTTCAGGT GGTGCCACCA CTTGGTTGTG TGAAGGTTAT CGGGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT