TrcrB.19501.b

JBP3 (glucosyl transferase interacting protein 2) (GTIP2)

CENTER ID:	TrcrB.19501.b
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19501.b.B2.GE40730	C-term domain		334	620
TrcrB.19501.b.B4.GE41052	N-extended J-binding domain		84	279

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19501.b.B4.PS38208	N-extended J-binding domain		84	279

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79391

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.510967.16
UniProt:	Q4CUX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEKEEESMA SRSVSSSYSD DSSYSSSTRS DNSSSGSGSG SGSSTTSSEE GVETQTGARR APTRPAAGDS ATSAATAVTS TPCSGLRSLQ LNPELVFEQP DASWIDIPHG SGSVFRRVVT NYDDFPERRY RVIDYFRTAV EMFVTQVRQE YTHQYQQAKK NDRANIRFIW RNKGELAICF AACCDMLKLL YDRFRPGLER PNWDGIVGQF VLFLMAESRI PAAVLTEQTY KTKFLDWRKG GTRYQGEQLT SNVRFPSAEE RRTKIETYLR SGEGYSIEST GVDLPSVVRP VPTHVKMTDA PVGQEETRNN GAFDVSNLAS PLVGFSRARP PVKMRSKSHM KPPQAVIAGK QSVEEYRHLH WLAKLTQITG CSPTLEFPAE RSYMILAFVR LCETSEMPEQ FATIMNILVR SSLTIQSAFE RGGGMVVLRR FVGTLVRLKD ACGLINLLEQ ILRLKLSSWS HSSQQSWLRD LLGNSGNDLR WLRALDIVPP DKQATWSSLI TALEQRYVLS NSREEEVRRG AKRARLDNDE ALRMSMADNV GVSKLAPYIS TAFCVSLPGE FCAPPLSSDM EQDTISLLNK KLQYCEEAQV TWRDAILKAL GGSCPGIPIP LWYDPYQLLG VDPYPAN

NT Sequence

atggcagaaa aagaagaaga gagcatggcg tcgaggtcgg tctcgagctc ctactcggat gactcgtcgt attcctcctc cacacgcagc gacaacagca gcagcggcag cggcagcggc agcggcagct ccacgacgtc ctccgaggaa ggcgtggaaa cccagacggg ggctcggcgt gcgccaacgc ggcctgcggc tggtgattcg gcaacttccg ccgccaccgc cgtcacatcg acgccgtgtt ccggactccg ctctctgcaa ctgaacccgg aacttgtgtt tgagcagccc gatgccagct ggattgacat accacacggc tctggtagtg tttttcgtcg cgtcgtaaca aactacgatg actttcccga gagacgctac cgtgtcattg actactttcg caccgccgta gaaatgttcg tcacgcaggt gcgccaagaa tacacgcatc agtatcagca ggcaaagaaa aacgaccgag caaacattcg cttcatctgg cgaaacaagg gggaattagc gatctgcttt gcggcgtgct gcgacatgct taaacttctc tacgatagat ttcgccccgg gcttgaaaga ccgaactggg atggaattgt aggtcagttt gttctttttt tgatggctga gtccaggata ccggccgccg ttttgacgga acagacgtac aagaccaaat ttcttgattg gcggaagggc ggtacccgtt atcagggcga gcaactgaca tcaaacgtcc gttttccttc cgcagaggaa cgtcgcacaa agatcgagac ctaccttcgc tcaggggaag gatacagcat agaatcaaca ggggtggacc ttccgtcggt ggtccgtccc gtccccacgc acgtgaaaat gacagatgca ccggttgggc aggaagagac gcgaaacaac ggcgcatttg atgtaagtaa tttggcttct ccccttgttg gcttttcacg tgccaggccc cccgtaaaga tgcgaagcaa atcacacatg aaacccccac aggcagtcat tgctgggaaa caatctgtag aagaatacag gcatttacat tggcttgcaa agctcaccca aatcactggc tgctctccaa cacttgaatt tccggctgag cggtcttaca tgatactggc gtttgttcga ctttgcgaaa catccgagat gcctgagcaa tttgcgacta ttatgaatat attggttagg tctagtctaa cgattcaaag cgcatttgag cggggtgggg gtatggtggt gctgcgtcga tttgtaggca cactggttcg gctgaaagat gcctgtgggt tgataaattt attggagcaa atactgcgac tgaaactttc ttcttggagc cacagcagcc aacagtcgtg gcttcgggac cttttgggaa atagtggaaa tgacttgcga tggttgcgtg ctctggacat agtgccgcca gacaagcaag ctacctggag ttcgttaata acggcgttgg agcaacgcta tgttctgtca aattcaagag aagaagaagt acggcgtggg gcgaagcgtg cacgattaga taatgacgag gcgttgcgca tgtctatggc ggacaatgtt ggtgtaagca agcttgcgcc ctatatttcc actgcctttt gcgtctcatt acccggcgag ttttgtgctc cgcctctttc ctcagacatg gagcaggaca ctatttcttt gttgaataag aagctgcagt actgcgagga ggcgcaggtg acgtggcgtg acgccatctt gaaggcgctc ggtggcagtt gcccaggcat acccattcct ctctggtacg acccgtatca attgcttggt gtggatccct atcctgcgaa ttag

Details for TrcrB.19501.b.B2.GE40730

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	33kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR SKSHMKPPQA VIAGKQSAEE YRHLHWLAKL TQITGCSPTL EFPAERSYMI LAFVRLCETS EMPEQFVTIM NILVRSSLTI QSAFERGGGM VVLRRFVGTL VRLKDACGLI NLLEQILRLK LSSWSHSSQQ SWLRDLLGNS GNDLRWLRAL DIVPPDKQAT WSSLITALEQ RYVLSNSREE EVRRGAKRAR LDNDEALRMS MADNVGVSKL APYISTAFCV SLPGEFCAPP LSSDMEQDTI SLLNKKLQYC EEAQVTWRDA ILKALGGSCP GIPIPLWYXX YQLLG

Validated NT Sequence

atggctcacc accaccacca ccacatgcga agcaaatcac acatgaaacc cccacaggca gtcattgctg ggaaacaatc tgcagaagaa tacagacatt tacattggct tgcaaagctt acccaaatca ctggttgctc tccaacactt gaatttccgg ctgagcggtc ttacatgata ctggcgtttg ttcgactttg cgaaacatcc gagatgcctg agcaatttgt gactattatg aatatattgg ttaggtctag tctaacgatt caaagcgcat ttgagcgggg tgggggtatg gtggtgctgc gtcgatttgt aggcacactg gttcggctga aagatgcctg tgggttgata aatttattgg agcaaatact gcgactgaaa ctttcttctt ggagccacag cagccaacag tcgtggcttc gggacctttt gggaaatagt ggaaatgact tgcgatggtt gcgtgctctg gacatagtgc cgccggacaa gcaagctacc tggagttcgt taataacggc gttggagcaa cgctatgttc tgtcaaattc aagagaagaa gaagtacggc gtggggcgaa gcgtgcacga ttagataatg acgaggcgtt gcgcatgtct atggcggaca atgttggtgt aagcaagctt gcgccctata tttccactgc cttttgcgtc tcattacccg gcgagttttg tgctccgcct ctttcctcag acatggagca ggacactatt tctttgttga ataagaagct gcagtactgc gaggaggcgc aggtgacgtg gcgtgacgcc atcttgaagg cgctcggtgg cagttgccca ggcataccca ttcctctctg gtacgnnnnn tatcaattgc ttggttaagt gagtaa

Expected Protein Sequence

MAHHHHHHMR SKSHMKPPQA VIAGKQSVEE YRHLHWLAKL TQITGCSPTL EFPAERSYMI LAFVRLCETS EMPEQFATIM NILVRSSLTI QSAFERGGGM VVLRRFVGTL VRLKDACGLI NLLEQILRLK LSSWSHSSQQ SWLRDLLGNS GNDLRWLRAL DIVPPDKQAT WSSLITALEQ RYVLSNSREE EVRRGAKRAR LDNDEALRMS MADNVGVSKL APYISTAFCV SLPGEFCAPP LSSDMEQDTI SLLNKKLQYC EEAQVTWRDA ILKALGGSCP GIPIPLWYDP YQLLG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa gcaaatcaca catgaaaccc ccacaggcag tcattgctgg gaaacaatct gtagaagaat acaggcattt acattggctt gcaaagctca cccaaatcac tggctgctct ccaacacttg aatttccggc tgagcggtct tacatgatac tggcgtttgt tcgactttgc gaaacatccg agatgcctga gcaatttgcg actattatga atatattggt taggtctagt ctaacgattc aaagcgcatt tgagcggggt gggggtatgg tggtgctgcg tcgatttgta ggcacactgg ttcggctgaa agatgcctgt gggttgataa atttattgga gcaaatactg cgactgaaac tttcttcttg gagccacagc agccaacagt cgtggcttcg ggaccttttg ggaaatagtg gaaatgactt gcgatggttg cgtgctctgg acatagtgcc gccagacaag caagctacct ggagttcgtt aataacggcg ttggagcaac gctatgttct gtcaaattca agagaagaag aagtacggcg tggggcgaag cgtgcacgat tagataatga cgaggcgttg cgcatgtcta tggcggacaa tgttggtgta agcaagcttg cgccctatat ttccactgcc ttttgcgtct cattacccgg cgagttttgt gctccgcctc tttcctcaga catggagcag gacactattt ctttgttgaa taagaagctg cagtactgcg aggaggcgca ggtgacgtgg cgtgacgcca tcttgaaggc gctcggtggc agttgcccag gcatacccat tcctctctgg tacgacccgt atcaattgct tggttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for TrcrB.19501.b.B4.GE41052

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS GLRSLQLNPE LVFEQPDASW IDIPHGSGSV FRRVVTNYDD FPERRYRVID YFRTAVEMFV TQVRQEYTHQ YQQAKKNDRA NIRFIWRNKG ELAICFAACC DMLKLLYDRF RPGLERPNWD GIVGQFVLFL MAESRIPAAV LTEQTYKTKF LDWRKGGTRY QGEQLTSNVR FPSAEERRTK IETYLRSGEG YSIES

Validated NT Sequence

atggctcacc accaccacca ccatatgtcc ggactccgct ctctgcaact gaacccggaa cttgtgtttg agcagcccga tgccagctgg attgacatac cacacggctc tggtagtgtt tttcgtcgcg tcgtaacaaa ctacgatgac tttcccgaga gacgctaccg tgtcattgac tactttcgca ccgccgtaga aatgttcgtc acgcaggtgc gccaagaata cacgcatcag tatcagcagg caaagaaaaa cgaccgagca aacattcgct tcatctggcg aaacaagggg gaattagcga tctgctttgc ggcgtgctgc gacatgctta aacttctcta cgatagattt cgccccgggc ttgaaagacc gaactgggat ggaattgtag gtcagtttgt tctttttttg atggctgagt ccaggatacc ggccgccgtt ttgacggaac agacgtacaa gaccaaattt cttgattggc ggaagggcgg tacccgttat cagggcgagc aactgacatc aaacgtccgt tttccttccg cagaggaacg tcgcacaaag atcgagacct accttcgctc aggggaagga tacagcatag aatcataagt gagtaagat

Expected Protein Sequence

MAHHHHHHSG LRSLQLNPEL VFEQPDASWI DIPHGSGSVF RRVVTNYDDF PERRYRVIDY FRTAVEMFVT QVRQEYTHQY QQAKKNDRAN IRFIWRNKGE LAICFAACCD MLKLLYDRFR PGLERPNWDG IVGQFVLFLM AESRIPAAVL TEQTYKTKFL DWRKGGTRYQ GEQLTSNVRF PSAEERRTKI ETYLRSGEGY SIES

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccggac tccgctctct gcaactgaac ccggaacttg tgtttgagca gcccgatgcc agctggattg acataccaca cggctctggt agtgtttttc gtcgcgtcgt aacaaactac gatgactttc ccgagagacg ctaccgtgtc attgactact ttcgcaccgc cgtagaaatg ttcgtcacgc aggtgcgcca agaatacacg catcagtatc agcaggcaaa gaaaaacgac cgagcaaaca ttcgcttcat ctggcgaaac aagggggaat tagcgatctg ctttgcggcg tgctgcgaca tgcttaaact tctctacgat agatttcgcc ccgggcttga aagaccgaac tgggatggaa ttgtaggtca gtttgttctt tttttgatgg ctgagtccag gataccggcc gccgttttga cggaacagac gtacaagacc aaatttcttg attggcggaa gggcggtacc cgttatcagg gcgagcaact gacatcaaac gtccgttttc cttccgcaga ggaacgtcgc acaaagatcg agacctacct tcgctcaggg gaaggataca gcatagaatc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for TrcrB.19501.b.B4.PS38208

PURIFICATION DATe:	2/22/2017
CONCENTRATION:	21.53mg/ml
OBSERVED MW:	24kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	31
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCCGGAC TCCGCTCTCT GCAACTGAAC CCGGAACTTG TGTTTGAGCA GCCCGATGCC AGCTGGATTG ACATACCACA CGGCTCTGGT AGTGTTTTTC GTCGCGTCGT AACAAACTAC GATGACTTTC CCGAGAGACG CTACCGTGTC ATTGACTACT TTCGCACCGC CGTAGAAATG TTCGTCACGC AGGTGCGCCA AGAATACACG CATCAGTATC AGCAGGCAAA GAAAAACGAC CGAGCAAACA TTCGCTTCAT CTGGCGAAAC AAGGGGGAAT TAGCGATCTG CTTTGCGGCG TGCTGCGACA TGCTTAAACT TCTCTACGAT AGATTTCGCC CCGGGCTTGA AAGACCGAAC TGGGATGGAA TTGTAGGTCA GTTTGTTCTT TTTTTGATGG CTGAGTCCAG GATACCGGCC GCCGTTTTGA CGGAACAGAC GTACAAGACC AAATTTCTTG ATTGGCGGAA GGGCGGTACC CGTTATCAGG GCGAGCAACT GACATCAAAC GTCCGTTTTC CTTCCGCAGA GGAACGTCGC ACAAAGATCG AGACCTACCT TCGCTCAGGG GAAGGATACA GCATAGAATC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT