TrcrB.19310.a

Phosphatidylinositol-4-phosphate 5-kinase alpha, putative (Pi4Ka)

CENTER ID: TrcrB.19310.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.19310.a.B1.GE42098 full length 1 475
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tc00.1047053504057.104
UniProt: Q4DSE4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPYNREIIAS ISFESEPSSR NNSPIRGGLN DNVKARESLD ATGEPVSQQN EATRDIIRCM KWALAQSITS ISHPAKQRPL NPKKDFTIET TIEVEVKKNN TLGLGCCGGA AAVKRRSSQV MSVRESGNSK VNFTYSDTDG ADDEEGPQVS FKFTDYSPMC YRHIREFFNI EPRAYCDVLM RSRWHSTPTP GKSAAQLFFC GKDWVIKTMT TEESEFLRSI LHRYYFFVLD NPHTLLPHFV GHHRLEIAGD KINFIIMQNV FATGNAIHEK YDLKGSTVGR FATEAEKRKQ TCTQKDLDLN RPIHVGPARR QQLIEQINKD CDFLKRSHVM DYSFLVGIHV LPMHDSRRLG TSVMGGKESI NMLSSTLAFD GTLASGVLDG QFNQTNGVFH ISPNEPVIDG RCFTADEGGM RSTDQPGTRR EIYYIGIIDI LQKYNARKYL ETSWFGLVND SKKISSVPPR EYAERFLAFV SSIIV
NT Sequence
atgccataca acagggaaat aattgcctcc atctcatttg agtctgagcc atcctctagg aataactcgc ccatacgagg cggcctcaat gacaacgtga aggcgcgaga gtcacttgat gctacaggag aacccgtttc ccaacaaaac gaggcaacgc gtgacattat tcgttgtatg aaatgggccc tggcgcagag tatcacgagt atttctcatc cagccaagca gcgacctctg aacccgaaga aagacttcac catagaaaca acgattgagg tcgaagtgaa aaaaaacaat acattagggt taggctgttg tggtggtgca gccgcggtga agcggcgttc ttcacaagtc atgtccgttc gcgagagtgg aaatagtaaa gttaatttta cttatagtga tactgacggt gctgacgatg aggaaggtcc acaagtttcc tttaaattta cggactattc tccaatgtgt tatcgtcata tacgggaatt tttcaatatt gagccgaggg cttattgtga tgttttgatg cgcagtagat ggcatagtac gcctacgcca gggaagtcgg ctgcacagct ctttttctgt ggtaaggact gggttataaa aaccatgacc acagaggaaa gtgagttttt gcgtagtatc ttgcatcgct actacttttt tgtgctggat aatccacaca cgttactgcc ccattttgtt gggcatcatc gccttgaaat tgctggggat aagataaatt ttattataat gcaaaatgtc tttgcaacag ggaatgcaat acatgaaaaa tatgatctta aaggaagtac ggtaggacgt tttgctaccg aagccgaaaa aagaaaacag acatgcacac aaaaagatct tgatttaaac cgtccaatac atgtggggcc ggcgcgaaga caacaactta ttgagcaaat caataaggac tgtgattttt tgaaacgatc acacgtcatg gattattcgt ttctggttgg tattcatgta ttgccgatgc acgactcacg gagattgggg actagtgtaa tggggggaaa agaaagcata aatatgttat cgtcaacatt agcctttgat ggcacgctag ctagtggggt tttagatgga caatttaacc agacaaatgg tgtttttcac atttccccga acgagcctgt cattgacgga agatgcttta ctgccgatga aggtggcatg cgtagcacgg accagccagg tactcggagg gaaatatatt acattggaat cattgatatc ttgcagaagt ataatgcacg gaaatattta gagacgtcat ggtttggttt ggtaaacgat tcaaagaaga tatcttccgt ccctcctcgc gaatacgcgg agcgtttttt ggcctttgtt tcctcaatta ttgtgtga
Details for TrcrB.19310.a.B1.GE42098
HARVESTED ON: 12/5/2017
SEQUENCED ON: 12/7/2017
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MPYNREIIAS ISFESEPSSR NNSPIRGGLN ENVKARESLD ATGEPVSQQN EATRDIIRCM KWALAQSITS ISHPAKQRPL NPKKDFTIET TIEVEVKNND TLGLGCCGGA AAVKRRSSQF MSVRESGNSK VNFTYSDTDG ADDEEGPQVS FKFTDYSPMC YRHIREFFNI EPRAYCDVLM RSRWHSTPTP GKSAAQLFFC GKDWVIKTMT TEESEFLRSI LHRYYFFVLD NPHTLLPHFV GHHRLEIAGD KINFIIMQNV FATGNAIHEK YDLKGSTVGR FATEAEKRKQ TCTQKDLDLN RPIHVGPARR QQLIEQINKD CDFLKRSHVM DYSFLVGIHV LPMHDSRRLG TSVMGGKESI NMLSSTLAFD GTLASGVLDG QFNQTNGVFH ISPNEPVIDG RCFTADEGGM RSTDQPGTRR EIYYIGIIDI LQKYNARKYL ETSWFGLVND SKKISSVPPR EYAERF
Validated NT Sequence
caaaaaacgc tccgcgtatt cgcgaggagg gacggaagat atcttctttg aatcgtttac caaaccaaac catgacgtct ctaaatattt ccgtgcatta tacttctgca agatatcaat gattccaatg taatatattt ccctccgagt acctggctgg tccgtgctac gcatgccacc ttcatcggca gtaaagcatc ttccgtcaat gacaggctcg ttcggggaaa tgtgaaaaac accatttgtc tggttaaatt gtccatctaa aaccccacta gctagcgtgc catcaaaggc taatgttgac gataacatat ttatgctttc ttttcccccc attacactag tccccaatct ccgtgagtcg tgcatcggca atacatgaat accaaccaga aacgaataat ccatgacgtg tgatcgtttc aaaaaatcac agtccttatt gatttgctca ataagttgtt gtcttcgcgc cggccccaca tgtattggac ggtttaaatc aagatctttt tgtgtgcatg tctgttttct tttttcggct tcggtagcaa aacgtcctac cgtacttcct ttaagatcat atttttcatg tattgcattc cctgttgcaa agacattttg cattataata aaatttatct tatccccagc aatttcaaga cgatgatgcc caacaaaatg gggcagtaac gtgtgtggat tatccagcac aaaaaagtag tagcgatgca agatactacg caaaaattca ctttcctctg tggtcatggt ttttataacc cagtccttac cacagaaaaa gagctgtgca gccgacttcc ctggcgtagg cgtactatgc catctactgc gcatcaaaac atcacaataa gcccttggct caatattgaa aaattcccgt atatgacgat aacacattgg agaatagtcc gtaaatttaa aggaaacttg tggaccttcc tcatcgtcag caccgtcagt atcactataa gtaaaattaa ctttgctatt tccactctcg cgaacggaca tgaattgtga agaacgccgc ttcaccgcgg ctgcaccacc acaacagcct aaccctaatg tatcgttgtt tttcacttcg acctcaatcg ttgtttctat ggtgaagtct ttcttcgggt tcagaggtcg ctgcttggct ggatgagaaa tactcgtgat actctgcgcc agggcccatt tcatacagcg aataatgtca cgtgttgcct cgttttgttg ggaaacgggt tctcctgtag cgtcaagtga ctctcgcgcc ttcacgtttt cattgaggcc gcctcgtatg ggcgagttat tcctagagga tggctcagac tcaaatgaga tggaggcaat tatttccctg ttgtatggca tatggtggtg gtggtggtga
Expected Protein Sequence
MAHHHHHHMP YNREIIASIS FESEPSSRNN SPIRGGLNDN VKARESLDAT GEPVSQQNEA TRDIIRCMKW ALAQSITSIS HPAKQRPLNP KKDFTIETTI EVEVKKNNTL GLGCCGGAAA VKRRSSQVMS VRESGNSKVN FTYSDTDGAD DEEGPQVSFK FTDYSPMCYR HIREFFNIEP RAYCDVLMRS RWHSTPTPGK SAAQLFFCGK DWVIKTMTTE ESEFLRSILH RYYFFVLDNP HTLLPHFVGH HRLEIAGDKI NFIIMQNVFA TGNAIHEKYD LKGSTVGRFA TEAEKRKQTC TQKDLDLNRP IHVGPARRQQ LIEQINKDCD FLKRSHVMDY SFLVGIHVLP MHDSRRLGTS VMGGKESINM LSSTLAFDGT LASGVLDGQF NQTNGVFHIS PNEPVIDGRC FTADEGGMRS TDQPGTRREI YYIGIIDILQ KYNARKYLET SWFGLVNDSK KISSVPPREY AERFLAFVSS IIV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccat acaacaggga aataattgcc tccatctcat ttgagtctga gccatcctct aggaataact cgcccatacg aggcggcctc aatgacaacg tgaaggcgcg agagtcactt gatgctacag gagaacccgt ttcccaacaa aacgaggcaa cgcgtgacat tattcgttgt atgaaatggg ccctggcgca gagtatcacg agtatttctc atccagccaa gcagcgacct ctgaacccga agaaagactt caccatagaa acaacgattg aggtcgaagt gaaaaaaaac aatacattag ggttaggctg ttgtggtggt gcagccgcgg tgaagcggcg ttcttcacaa gtcatgtccg ttcgcgagag tggaaatagt aaagttaatt ttacttatag tgatactgac ggtgctgacg atgaggaagg tccacaagtt tcctttaaat ttacggacta ttctccaatg tgttatcgtc atatacggga atttttcaat attgagccga gggcttattg tgatgttttg atgcgcagta gatggcatag tacgcctacg ccagggaagt cggctgcaca gctctttttc tgtggtaagg actgggttat aaaaaccatg accacagagg aaagtgagtt tttgcgtagt atcttgcatc gctactactt ttttgtgctg gataatccac acacgttact gccccatttt gttgggcatc atcgccttga aattgctggg gataagataa attttattat aatgcaaaat gtctttgcaa cagggaatgc aatacatgaa aaatatgatc ttaaaggaag tacggtagga cgttttgcta ccgaagccga aaaaagaaaa cagacatgca cacaaaaaga tcttgattta aaccgtccaa tacatgtggg gccggcgcga agacaacaac ttattgagca aatcaataag gactgtgatt ttttgaaacg atcacacgtc atggattatt cgtttctggt tggtattcat gtattgccga tgcacgactc acggagattg gggactagtg taatgggggg aaaagaaagc ataaatatgt tatcgtcaac attagccttt gatggcacgc tagctagtgg ggttttagat ggacaattta accagacaaa tggtgttttt cacatttccc cgaacgagcc tgtcattgac ggaagatgct ttactgccga tgaaggtggc atgcgtagca cggaccagcc aggtactcgg agggaaatat attacattgg aatcattgat atcttgcaga agtataatgc acggaaatat ttagagacgt catggtttgg tttggtaaac gattcaaaga agatatcttc cgtccctcct cgcgaatacg cggagcgttt tttggccttt gtttcctcaa ttattgtgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat