SSGCID: Target Page

TrcrB.18889.b

alanyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18889.b
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18889.b.B1.GE42714	full length		1	959

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.511825.220
UniProt:	Q4DQ33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTEWPVNRVR QEFLKFFEQR GHKFVPSSPV VPNNDPTLLF INAGMNQFKN LFLGTADPNT EFGRLTRAAN SQLCIRAGGK HNDLDDVGRD TYHHTFFEML GSWSFGDYFK RDAIRWAWEL LTEVYKLPKD RLYVTYFEGD PENGIAADEE AKQLWLELVK PEHVISGNAK DNFWEMGDIG PCGPCSEVHF DRLGGRNAAE LVNKEDPMVI EIWNLVFMQF ERHEDGVLVP LPKMHVDTGM GLERLTSIMQ DVHSNYDTDA WTPLFEAIQA VTGYEKSYAE IRDQNDSDAT VAYRVIADHI RCLSVSLADG AMPDSVGRGF VLRRIIRRAV RYGLQFLGAK VGFFSELVDA VVASLGPFFT HMQDPRTVQR IKTVLADEEA SFAKTWDVGL KHLNKAVADA VDNTISGDNA FILHDRYGFP VDLTCLLAEK AGLRVDMNGF HAAMKANQLS AGRVAAAKTF LDVHQLEELK QMGIAPTEDA AKYIWKNRTA EVKAIFNKKT GTFVDMLMPG EEGEEDIGLI MDVTNFYAES GGQIYDTGRI VAAADAVFEV KKVYNLGGYV VHLGRMMTTN STAVPIPVTS SVDLQVDYKR RIAIAANHSM THVLNWCLRR VLEDEAPENY MEVQQKGSLV TEEMLRFDFS YNSKVSQEDL VKIEKALNEK IQQGLVVYRK EVELEKAKHI VGLRQMFGEK YPDPVSVISV GVPVDDLLAK PESAEWRAYA VEFCGGTHLS NIKEAEVAVI LSEEALMKGV RRIVVATRDA AREARARGEL LEKEYRDIVA GGMTDSTAKS LSVLNKKVGD SLIPLTVKNS LREEIDGSVR AAIANVKAMA AQLREKATVA GRAEAEAYDA VENPFFVKRL SDFGAEREAL QAYADGFTNA VQGDVGVFLF GTDANKALAL VTLPKAFTAK KLSAVDWAKA AIGKGGGKPH AAQSGLTAAD VDVAMEKALA EAAKMKTAM

NT Sequence

atgactgagt ggcccgtcaa ccgtgtgcgc caggagtttc tgaaattctt tgagcagcga ggacacaagt ttgtcccatc tagccctgtc gtgccaaaca atgatcccac actgctcttt atcaatgccg gcatgaatca atttaaaaat ctttttctcg gcacggcaga tcccaatacg gaatttggtc gccttacccg tgcggcgaac tcacaacttt gtattcgcgc aggtggtaaa cacaatgatc tggacgatgt tggtcgtgat acttaccatc acaccttttt tgagatgttg ggttcatggt cgtttggtga ctattttaaa cgcgacgcaa tccgctgggc atgggaactt ttgaccgagg tgtacaagct tccaaaggac cgtctttatg tgacatactt tgaaggggac cctgaaaacg gcatagcagc cgatgaagag gcgaaacaat tgtggttgga gcttgtgaaa cccgagcatg ttatatctgg taatgccaaa gacaactttt gggaaatggg cgatatagga ccttgtggac cttgctcgga agttcacttt gatcgtctgg gtggtcgcaa tgcggccgaa ttggtgaata aggaagatcc aatggtgatc gagatttgga accttgtttt tatgcaattt gagcgtcatg aggacggtgt gcttgtaccg ttgccgaaga tgcatgtaga tacaggcatg gggctcgagc gactgacgtc cattatgcaa gatgtgcatt cgaactacga tacagacgcc tggacgccac tctttgaggc cattcaggcg gtaacgggct acgaaaagtc atatgccgaa atacgtgatc aaaatgactc cgatgcaacc gtggcgtacc gtgtgatagc agatcacatt cgctgcctca gtgtttccct ggcggatggc gccatgcccg attctgttgg tcgtggtttt gtgttgcgcc gcataatccg ccgcgcagtc cgctacggtt tgcagttcct tggcgcaaag gttggcttct ttagtgaact tgttgatgct gttgtggcgt cgttggggcc ctttttcacg cacatgcaag atcctcgcac ggtgcagcgt ataaaaactg tgcttgccga cgaggaggca tccttcgcca agacatggga tgtaggtctt aagcacttaa acaaggccgt tgctgacgca gttgacaaca ccatcagtgg tgataatgcc tttattcttc acgatcgcta tggcttccct gtggatttga cctgtctgct ggcggagaag gcaggattac gtgtggatat gaacggcttt cacgcggcca tgaaggcaaa tcaactaagt gctggtcgtg tcgctgctgc aaagacattc ctcgatgttc accaactgga ggagctcaaa cagatgggta tcgccccaac ggaggatgcc gcaaagtaca tctggaagaa tcgtaccgca gaggtgaagg ccatttttaa caagaagaca ggaacgtttg tagacatgct gatgcctgga gaggaaggcg aggaagatat cggcctcatt atggatgtca caaactttta cgcggagtct ggtggccaaa tatacgacac tgggcgcatt gtagctgctg ccgatgccgt gtttgaagta aagaaggttt ataatcttgg tggctacgtg gtacatttgg gacgcatgat gacaacaaac tcaacggctg tacctattcc tgtaacgtct tctgtggatt tgcaggttga ttacaaacgg cggatagcga ttgctgcgaa tcactccatg acacacgtcc tgaactggtg tctacgacgc gtgctagagg acgaagctcc tgagaattac atggaggttc aacagaaggg gtcccttgtc accgaagaaa tgctacgctt tgacttcagc tacaatagta aggtcagtca agaagatctc gtcaagatcg agaaggccct taatgagaaa attcaacagg ggctagtggt gtaccgcaag gaggtggaac ttgaaaaagc caaacatatt gtggggctgc ggcagatgtt cggcgagaag tacccagacc ccgtcagtgt catttctgtt ggcgtcccag tcgatgacct tcttgcaaag cctgagagtg cagagtggcg tgcgtacgct gtggaatttt gtggtggtac ccacttgtcg aacataaaag aggcagaggt tgcggttatc ctttcagagg aagcacttat gaaaggggtt cgacggattg ttgtggcgac acgcgacgcc gcacgcgagg cccgtgcacg gggggaactg ctggagaagg aatatcgtga tatcgtcgcc ggtggtatga cggattcaac cgctaagagc ctctcggtcc tgaacaagaa ggtcggtgac agtctcatcc cgctcacggt gaaaaattca ctgcgggagg aaatcgacgg gagcgtccgg gctgccattg ccaatgtgaa ggcgatggct gcgcaactga gggagaaggc caccgttgca ggacgagcgg aggcggaggc gtatgacgcg gtggaaaacc ccttttttgt taagcgactc agcgattttg gtgctgaacg cgaggcactg caggcatacg ctgatggatt tacaaacgcc gtgcagggcg acgttggtgt ttttctcttc ggcaccgatg cgaacaaggc actcgcgctg gtgacgcttc caaaggcgtt tacggcgaag aagctaagcg ctgtcgattg ggcaaaggca gcaatcggga aaggtggcgg taagccgcac gccgcacagt caggccttac cgctgccgac gtcgatgtgg cgatggagaa ggcgcttgca gaggccgcaa agatgaagac tgcgatgtga

Details for TrcrB.18889.b.B1.GE42714

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	107kDa
OBSERVED MW:	107kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT EWPVNRVRQE FLKFFEQRGH KFVPSSPVVP NNDPTLLFIN AGMNQFKNLF LGTADPNTEF GRLTRAANSQ LCIRAGGKHN DLDDVGRDTY HHTFFEMLGS WSFGDYFKRD AIRWAWELLT EVYKLPKDRL YVTYFEGDPE NGIAADEEAK QLWLELVKPE HVISGNAKDN FWEMGDIGPC GPCSEVHFDR LGGRNAAELV NKEDPMVIEI WNLVFMQFER HEDGVLVPLP KMHVDTGMGL ERLTSIMQDV HSNYDTDAWT PLFEAIQAVT GYEKSYAEIR DQNDSDATVA YRVIADHIRC LSVSLADGAM PDSVGRGFVL RRIIRRAVRY GLQFLGAKVG FFSELVDAVV ASLGPFFTHM QDPRTVQRIK TVLADEEASF AKTWDVGLKH LNKAVADAVD NTISGDNAFI LHDRYGFPVD LTCLLAEKAG LRVDMNGFHA AMKANQLSAG RVAAAKTFLD VHQLEELKQM GIAPTEDAAK YIWKNRTAEV KAIFNKKTGT FVDMLMPGEE GEEDIGLIMD VTNFYAESGG QIYDTGRIVA AADAVFEVKK VYNLGGYVVH LGRMMTTNST AVPIPVTSSV DLQVDYKRRI AIAANHSMTH VLNWCLRRVL EDEAPENYME VQQKGSLVTE EMLRFDFSYN SKVSQEDLVK IEKALNEKIQ QGLVVYRKEV ELEKAKHIVG LRQMFGEKYP DPVSVISVGV PVDDLLAKPE SAEWRAYAVE FCGGTHLSNI KEAEVAVILS EEALMKGVRR IVVATRDAAR EARARGELLE KEYRDIVAGG MTDSTAKSLS VLNKKVGDSL IPLTVKNSLR EEIDGSVRAA IANVKAMAAQ LREKATVAGR AEAEAYDAVE NPFFVKRLSD FGAEREALQA YADGFTNAVQ GDVGVFLFGT DANKALALVT LPKAFTAKKL SAVDWAKAAI GKGGGKPHAA QSGLTAADVD VAMEKALAEA AKMKTAM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg agtggcccgt caaccgtgtg cgccaggagt ttctgaaatt ctttgagcag cgaggacaca agtttgtccc atctagccct gtcgtgccaa acaatgatcc cacactgctc tttatcaatg ccggcatgaa tcaatttaaa aatctttttc tcggcacggc agatcccaat acggaatttg gtcgccttac ccgtgcggcg aactcacaac tttgtattcg cgcaggtggt aaacacaatg atctggacga tgttggtcgt gatacttacc atcacacctt ttttgagatg ttgggttcat ggtcgtttgg tgactatttt aaacgcgacg caatccgctg ggcatgggaa cttttgaccg aggtgtacaa gcttccaaag gaccgtcttt atgtgacata ctttgaaggg gaccctgaaa acggcatagc agccgatgaa gaggcgaaac aattgtggtt ggagcttgtg aaacccgagc atgttatatc tggtaatgcc aaagacaact tttgggaaat gggcgatata ggaccttgtg gaccttgctc ggaagttcac tttgatcgtc tgggtggtcg caatgcggcc gaattggtga ataaggaaga tccaatggtg atcgagattt ggaaccttgt ttttatgcaa tttgagcgtc atgaggacgg tgtgcttgta ccgttgccga agatgcatgt agatacaggc atggggctcg agcgactgac gtccattatg caagatgtgc attcgaacta cgatacagac gcctggacgc cactctttga ggccattcag gcggtaacgg gctacgaaaa gtcatatgcc gaaatacgtg atcaaaatga ctccgatgca accgtggcgt accgtgtgat agcagatcac attcgctgcc tcagtgtttc cctggcggat ggcgccatgc ccgattctgt tggtcgtggt tttgtgttgc gccgcataat ccgccgcgca gtccgctacg gtttgcagtt ccttggcgca aaggttggct tctttagtga acttgttgat gctgttgtgg cgtcgttggg gccctttttc acgcacatgc aagatcctcg cacggtgcag cgtataaaaa ctgtgcttgc cgacgaggag gcatccttcg ccaagacatg ggatgtaggt cttaagcact taaacaaggc cgttgctgac gcagttgaca acaccatcag tggtgataat gcctttattc ttcacgatcg ctatggcttc cctgtggatt tgacctgtct gctggcggag aaggcaggat tacgtgtgga tatgaacggc tttcacgcgg ccatgaaggc aaatcaacta agtgctggtc gtgtcgctgc tgcaaagaca ttcctcgatg ttcaccaact ggaggagctc aaacagatgg gtatcgcccc aacggaggat gccgcaaagt acatctggaa gaatcgtacc gcagaggtga aggccatttt taacaagaag acaggaacgt ttgtagacat gctgatgcct ggagaggaag gcgaggaaga tatcggcctc attatggatg tcacaaactt ttacgcggag tctggtggcc aaatatacga cactgggcgc attgtagctg ctgccgatgc cgtgtttgaa gtaaagaagg tttataatct tggtggctac gtggtacatt tgggacgcat gatgacaaca aactcaacgg ctgtacctat tcctgtaacg tcttctgtgg atttgcaggt tgattacaaa cggcggatag cgattgctgc gaatcactcc atgacacacg tcctgaactg gtgtctacga cgcgtgctag aggacgaagc tcctgagaat tacatggagg ttcaacagaa ggggtccctt gtcaccgaag aaatgctacg ctttgacttc agctacaata gtaaggtcag tcaagaagat ctcgtcaaga tcgagaaggc ccttaatgag aaaattcaac aggggctagt ggtgtaccgc aaggaggtgg aacttgaaaa agccaaacat attgtggggc tgcggcagat gttcggcgag aagtacccag accccgtcag tgtcatttct gttggcgtcc cagtcgatga ccttcttgca aagcctgaga gtgcagagtg gcgtgcgtac gctgtggaat tttgtggtgg tacccacttg tcgaacataa aagaggcaga ggttgcggtt atcctttcag aggaagcact tatgaaaggg gttcgacgga ttgttgtggc gacacgcgac gccgcacgcg aggcccgtgc acggggggaa ctgctggaga aggaatatcg tgatatcgtc gccggtggta tgacggattc aaccgctaag agcctctcgg tcctgaacaa gaaggtcggt gacagtctca tcccgctcac ggtgaaaaat tcactgcggg aggaaatcga cgggagcgtc cgggctgcca ttgccaatgt gaaggcgatg gctgcgcaac tgagggagaa ggccaccgtt gcaggacgag cggaggcgga ggcgtatgac gcggtggaaa accccttttt tgttaagcga ctcagcgatt ttggtgctga acgcgaggca ctgcaggcat acgctgatgg atttacaaac gccgtgcagg gcgacgttgg tgtttttctc ttcggcaccg atgcgaacaa ggcactcgcg ctggtgacgc ttccaaaggc gtttacggcg aagaagctaa gcgctgtcga ttgggcaaag gcagcaatcg ggaaaggtgg cggtaagccg cacgccgcac agtcaggcct taccgctgcc gacgtcgatg tggcgatgga gaaggcgctt gcagaggccg caaagatgaa gactgcgatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat