SSGCID: Target Page

TrcrB.18889.a

alanyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18889.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18889.a.B1.GE42713	full length		1	959

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.506863.10
UniProt:	Q4D747

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTEWPVNRVR QEFLKFFEQR GHKFVPSSPV VPNNDPTLLF INAGMNQFKN LFLGTADPNT EFGRLTRAAN SQLCIRAGGK HNDLDDVGRD TYHHTFFEML GSWSFGDYFK RDAIRWAWEL LTEVYKLPKD RLYVTYFEGD PENGIAADEE AKQLWLELVK PEHVISGNAK DNFWEMGDIG PCGPCSEVHF DRLGGRNAAE LVNKEDPMVI EIWNLVFMQF ERHEGGVLVP LPKMHVDTGM GLERLTSIMQ GVHSNYDTDA WTPLFEAIQA VTGYEKSYAE IRDQNDSDAT VAYRVIADHI RCLSVSLADG AMPDSVGRGF VLRRIIRRAV RYGLQFLGAK VGFFSELVDA VVASLGPFFT HMQDPRTVQR IKTVLADEEA SFAKTWDVGL KHFNKAVADA VDNTISGDNA FILHDRYGFP VDLTCLLAEN AGLRVDMNGF HAAMKANQLS AGRVAAAKTF LDVHQLEELK QMGIAPTEDA AKYIWKNRTA EVKAIFNKKT GTFVDMLMPG EEGEEDIGLI MDVTNFYAES GGQIYDTGRI VAAADAVFEV RKVYNLGGYV VHLGRMMTTN STAVPIPVTS SVDLQVDYKR RIAIAANHSM THVLNWCLRR VLEDEAPENY MEVQQKGSLV TEEMLRFDFS YNSKVSQEDL VKIEKALNEK IQQGLVVYRK EVELEKAKHI VGLRQMFGEK YPDPVSVISV GVPVDDLLAK PESAEWRAYA VEFCGGTHLS NIKESEVAVI LSEEALMKGV RRIVVATRDA ARKARARGEL LEKEYRDIVA GGMTDSTAKS LSVLNKKVGD SLIPLTVKNS LREEIDGSIR AALANVKAMA AQLREKATVA GRAEAEAYDA VENPFFVKRL SDFGAEREAL QAYADGFTNA VQGDVGVFLF GTDANKALAL VTLPKAFTAK KLSAVDWAKA AIGKGGGKPH AAQSGLTAAD VDVAMEKALA EAAKMKTAM

NT Sequence

atgactgagt ggcccgtcaa ccgtgtgcgc caggagtttc tgaaattctt tgagcagcga ggacacaagt ttgtcccatc tagccctgtc gtgccaaaca atgatcccac actgctcttt atcaatgccg gcatgaatca atttaaaaat ctttttcttg gcacggcaga tcccaatacg gaatttggtc gccttacccg tgcggcgaac tcacaacttt gtattcgcgc aggcggtaaa cacaatgatc tggacgatgt tggtcgtgat acttaccatc acaccttttt tgagatgttg ggttcatggt cgtttggtga ctattttaaa cgcgacgcaa tccgctgggc atgggaactt ttgaccgagg tgtacaagct tccaaaggac cgtctttatg tgacatactt tgagggggac cctgaaaacg gcatagcagc cgatgaagag gcgaaacaat tgtggttgga gcttgtgaaa cccgagcatg ttatatctgg taatgccaaa gacaactttt gggaaatggg cgatatagga ccttgtggac cttgctcgga agttcacttt gaccgtctgg gtggtcgcaa tgcggccgag ctggtgaata aggaagatcc aatggtgatc gagatttgga accttgtttt tatgcaattt gagcgtcatg aaggcggtgt gcttgtaccg ttgccgaaga tgcatgtaga tacaggcatg gggctcgagc gactgacgtc cattatgcaa ggtgtgcact cgaactacga tacagacgcc tggacgccac tctttgaggc cattcaggcg gtgacgggtt acgaaaagtc atatgccgaa atacgtgatc aaaatgactc cgatgcaacc gtggcgtacc gtgtgatagc agatcacatt cgctgcctca gtgtttccct ggcggatggt gccatgcccg attctgttgg tcgtggtttt gtgttgcgcc gcatcatccg ccgcgcagtc cgctacggtt tgcagttcct tggcgcaaag gttggcttct ttagtgaact tgttgacgct gttgtggcgt cgttggggcc ctttttcacg cacatgcaag atcctcgcac agtgcagcgt atcaaaactg tgcttgccga cgaggaggca tcctttgcca agacatggga tgtgggtctt aagcacttca acaaggccgt tgctgacgcg gttgacaaca ccatcagtgg tgataatgcc tttattcttc acgatcgcta tggcttccct gtggatttga cctgtttgct ggcggagaat gcagggttac gtgtggatat gaacggcttt cacgcggcca tgaaggcaaa tcaactaagt gctggtcgtg tcgctgctgc aaagacattc ctcgatgttc accaactgga ggagctcaaa cagatgggta tcgccccaac ggaggatgcc gccaagtaca tctggaagaa tcgtaccgca gaggtgaagg ccatttttaa caagaagaca ggaacgtttg tagacatgct gatgcctgga gaggaaggcg aggaagatat cggcctcatt atggatgtca caaactttta cgcggagtct ggtggccaaa tatacgacac tgggcgcatt gtagctgctg ccgatgccgt gtttgaagta aggaaggttt ataatcttgg tggctacgtg gtacatttgg gacgcatgat gacaacaaac tcaacggctg tacctattcc tgtaacgtct tctgtggatt tgcaggtgga ttacaaacgg cggatagcga ttgctgcgaa tcactccatg acacacgtcc tgaactggtg tctacgacgc gtgctagagg acgaagctcc tgagaattac atggaggttc aacagaaggg gtcccttgtc accgaagaaa tgctacgctt tgacttcagc tacaatagta aggtcagtca agaagatctc gtcaagatcg agaaggccct taatgagaaa attcaacagg ggctagtggt gtaccgcaag gaggtggaac ttgaaaaagc caaacatatt gtggggctgc ggcagatgtt cggcgagaag tacccagacc ccgtcagtgt catttctgtt ggcgtcccag tcgatgacct tcttgcaaag cctgagagtg cagagtggcg tgcgtacgct gtggaatttt gtggtggcac ccacttgtcg aacataaaag agtcagaggt tgcggttatc ctctcagagg aagcacttat gaaaggggtt cgacggattg ttgtggcgac acgcgacgcc gcacgcaagg cccgtgcacg gggggaactg ctggagaagg aatatcgtga tatcgtcgcc ggtggtatga cggattcaac cgctaagagc ctctcggtcc tgaacaagaa ggtcggcgac agtctcatcc cgctcacggt gaaaaattca ctgcgggagg aaatcgacgg gagtatccgg gctgcccttg ccaatgtgaa ggcgatggct gcgcaactga gggagaaggc caccgttgca ggacgagcgg aggcggaggc gtatgacgcg gtggaaaacc ccttttttgt taagcgactc agcgattttg gtgctgaacg agaggcactg caggcatacg ctgatggatt tacaaacgcc gtgcagggcg acgttggcgt ttttctcttc ggcaccgatg cgaacaaggc actcgcgctg gtgacgctcc caaaggcgtt tactgcgaag aagctaagcg ctgtcgattg ggcaaaggca gcaatcggga aaggtggcgg taagccgcac gccgcacagt caggccttac cgctgccgac gtcgatgtgg cgatggagaa ggcgcttgca gaggccgcaa agatgaagac tgcgatgtga

Details for TrcrB.18889.a.B1.GE42713

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	107kDa
OBSERVED MW:	107kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT EWPVNRVRQE FLKFFEQRGH KFVPSSPVVP NNDPTLLFIN AGMNQFKNLF LGTADPNTEF GRLTRAANSQ LCIRAGGKHN DLDDVGRDTY HHTFFEMLGS WSFGDYFKRD AIRWAWELLT EVYKLPKDRL YVTYFEGDPE NGIAADEEAK QLWLELVKPE HVISGNAKDN FWEMGDIGPC GPCSEVHFDR LGGRNAAELV NKEDPMVIEI WNLVFMQFER HEGGVLVPLP KMHVDTGMGL ERLTSIMQGV HSNYDTDAWT PLFEAIQAVT GYEKSYAEIR DQNDSDATVA YRVIADHIRC LSVSLADGAM PDSVGRGFVL RRIIRRAVRY GLQFLGAKVG FFSELVDAVV ASLGPFFTHM QDPRTVQRIK TVLADEEASF AKTWDVGLKH FNKAVADAVD NTISGDNAFI LHDRYGFPVD LTCLLAENAG LRVDMNGFHA AMKANQLSAG RVAAAKTFLD VHQLEELKQM GIAPTEDAAK YIWKNRTAEV KAIFNKKTGT FVDMLMPGEE GEEDIGLIMD VTNFYAESGG QIYDTGRIVA AADAVFEVRK VYNLGGYVVH LGRMMTTNST AVPIPVTSSV DLQVDYKRRI AIAANHSMTH VLNWCLRRVL EDEAPENYME VQQKGSLVTE EMLRFDFSYN SKVSQEDLVK IEKALNEKIQ QGLVVYRKEV ELEKAKHIVG LRQMFGEKYP DPVSVISVGV PVDDLLAKPE SAEWRAYAVE FCGGTHLSNI KESEVAVILS EEALMKGVRR IVVATRDAAR KARARGELLE KEYRDIVAGG MTDSTAKSLS VLNKKVGDSL IPLTVKNSLR EEIDGSIRAA LANVKAMAAQ LREKATVAGR AEAEAYDAVE NPFFVKRLSD FGAEREALQA YADGFTNAVQ GDVGVFLFGT DANKALALVT LPKAFTAKKL SAVDWAKAAI GKGGGKPHAA QSGLTAADVD VAMEKALAEA AKMKTAM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg agtggcccgt caaccgtgtg cgccaggagt ttctgaaatt ctttgagcag cgaggacaca agtttgtccc atctagccct gtcgtgccaa acaatgatcc cacactgctc tttatcaatg ccggcatgaa tcaatttaaa aatctttttc ttggcacggc agatcccaat acggaatttg gtcgccttac ccgtgcggcg aactcacaac tttgtattcg cgcaggcggt aaacacaatg atctggacga tgttggtcgt gatacttacc atcacacctt ttttgagatg ttgggttcat ggtcgtttgg tgactatttt aaacgcgacg caatccgctg ggcatgggaa cttttgaccg aggtgtacaa gcttccaaag gaccgtcttt atgtgacata ctttgagggg gaccctgaaa acggcatagc agccgatgaa gaggcgaaac aattgtggtt ggagcttgtg aaacccgagc atgttatatc tggtaatgcc aaagacaact tttgggaaat gggcgatata ggaccttgtg gaccttgctc ggaagttcac tttgaccgtc tgggtggtcg caatgcggcc gagctggtga ataaggaaga tccaatggtg atcgagattt ggaaccttgt ttttatgcaa tttgagcgtc atgaaggcgg tgtgcttgta ccgttgccga agatgcatgt agatacaggc atggggctcg agcgactgac gtccattatg caaggtgtgc actcgaacta cgatacagac gcctggacgc cactctttga ggccattcag gcggtgacgg gttacgaaaa gtcatatgcc gaaatacgtg atcaaaatga ctccgatgca accgtggcgt accgtgtgat agcagatcac attcgctgcc tcagtgtttc cctggcggat ggtgccatgc ccgattctgt tggtcgtggt tttgtgttgc gccgcatcat ccgccgcgca gtccgctacg gtttgcagtt ccttggcgca aaggttggct tctttagtga acttgttgac gctgttgtgg cgtcgttggg gccctttttc acgcacatgc aagatcctcg cacagtgcag cgtatcaaaa ctgtgcttgc cgacgaggag gcatcctttg ccaagacatg ggatgtgggt cttaagcact tcaacaaggc cgttgctgac gcggttgaca acaccatcag tggtgataat gcctttattc ttcacgatcg ctatggcttc cctgtggatt tgacctgttt gctggcggag aatgcagggt tacgtgtgga tatgaacggc tttcacgcgg ccatgaaggc aaatcaacta agtgctggtc gtgtcgctgc tgcaaagaca ttcctcgatg ttcaccaact ggaggagctc aaacagatgg gtatcgcccc aacggaggat gccgccaagt acatctggaa gaatcgtacc gcagaggtga aggccatttt taacaagaag acaggaacgt ttgtagacat gctgatgcct ggagaggaag gcgaggaaga tatcggcctc attatggatg tcacaaactt ttacgcggag tctggtggcc aaatatacga cactgggcgc attgtagctg ctgccgatgc cgtgtttgaa gtaaggaagg tttataatct tggtggctac gtggtacatt tgggacgcat gatgacaaca aactcaacgg ctgtacctat tcctgtaacg tcttctgtgg atttgcaggt ggattacaaa cggcggatag cgattgctgc gaatcactcc atgacacacg tcctgaactg gtgtctacga cgcgtgctag aggacgaagc tcctgagaat tacatggagg ttcaacagaa ggggtccctt gtcaccgaag aaatgctacg ctttgacttc agctacaata gtaaggtcag tcaagaagat ctcgtcaaga tcgagaaggc ccttaatgag aaaattcaac aggggctagt ggtgtaccgc aaggaggtgg aacttgaaaa agccaaacat attgtggggc tgcggcagat gttcggcgag aagtacccag accccgtcag tgtcatttct gttggcgtcc cagtcgatga ccttcttgca aagcctgaga gtgcagagtg gcgtgcgtac gctgtggaat tttgtggtgg cacccacttg tcgaacataa aagagtcaga ggttgcggtt atcctctcag aggaagcact tatgaaaggg gttcgacgga ttgttgtggc gacacgcgac gccgcacgca aggcccgtgc acggggggaa ctgctggaga aggaatatcg tgatatcgtc gccggtggta tgacggattc aaccgctaag agcctctcgg tcctgaacaa gaaggtcggc gacagtctca tcccgctcac ggtgaaaaat tcactgcggg aggaaatcga cgggagtatc cgggctgccc ttgccaatgt gaaggcgatg gctgcgcaac tgagggagaa ggccaccgtt gcaggacgag cggaggcgga ggcgtatgac gcggtggaaa accccttttt tgttaagcga ctcagcgatt ttggtgctga acgagaggca ctgcaggcat acgctgatgg atttacaaac gccgtgcagg gcgacgttgg cgtttttctc ttcggcaccg atgcgaacaa ggcactcgcg ctggtgacgc tcccaaaggc gtttactgcg aagaagctaa gcgctgtcga ttgggcaaag gcagcaatcg ggaaaggtgg cggtaagccg cacgccgcac agtcaggcct taccgctgcc gacgtcgatg tggcgatgga gaaggcgctt gcagaggccg caaagatgaa gactgcgatg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat