TrcrB.18849.c

valyl-tRNA synthetase, putative

CENTER ID: TrcrB.18849.c
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18849.c.B1.GE42709 full length 1 975
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18849.c.B1.PW38558 full length 1 975

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.509167.100
UniProt: Q4DQQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRLTPQMAA AYTPKDVESE WYEWWEKSGF FRPASDLGRK HNGNTFVIVS PPPNVTGYLH LGHSLTGAVQ DTLIRFHRMK GDNTLYVPGT DHAGIATQVV VEKRLLRESG KTRHDLGREE FLKRVWDFKE NHAHVITRQL RQIGLSLDWT RERFTMDDHC AGAVLEAFVK LHEDGLVRRS TRLVNWCCAL QSAISDLEVE FADVPKNAKI AIPGYDKKVD MGVLTHVAYR FEDSDDEIVI ATTRPETILG DTAVAVHPDD ERYKKYHGKR LKCPFRDETI PLILDPVLVD VSFGTGAVKI TPSHDPNDFE AGVRHNLPQL TMMDLNGRIT IDGPFKGMHR FDCRREIVKE LEKMGLLRDV VPYEYRVGRC SRTGDIVEPL LMPQWFVDCS EMARKSVEAV RNKELRLYPS SHEAVWFHWL ENIKPWCVSR QLWWGHRIPA YKCSGVVPSN HEDPWVVARN IAEARSKARA KFNLTDAEVE QLVLDQDPDV LDTWFSSAMW PYSTLGWPGD TDDMHKFFPG NLMETGHDIL FFWVARMVMT SLHFTGKLPF SEVFLHAMVR DKNGEKMSKS KGNVIDPLFI INGITLEGLH STVTNGNLDE KEVPRALKLQ KETFPNGIPE CGSDALRFGL LSYTQSGRSV NLDIDRIVAY RQFCNKLWNA VRYVLYHALG TEYKPGLTVI DVLRAESYPL ECRWILSRLD VAVEECTRGF SEGSYDFALT TNAAYRFWLY ELCDVFLELT KFSIHAGGDR KQLVQDVLLH VVEVALRLLH PMMPFLTEEL WHRLPNYNTF GVESIMLAPY PAVAGWKNDT VEEKMRIMMD TVRNVRSTKA SYSLTNKHKP DVWITAQTAE VKQLLMDHSY MVISLGVVGN VSVISPEEVE ANVPKGCGFS VVNKEVGVNM MLLGFIDVQK EVAKLDKQLV GLQKQIEGLK KKMSIPNYDM KVPADVRTAN SEKLDTLTAQ EKQILEGLEK MKSIL
NT Sequence
atgaagcggc tgaccccgca gatggctgcg gcctataccc ccaaggatgt ggagagtgaa tggtatgagt ggtgggaaaa gtccggcttc ttccgccctg catcggattt gggccgcaag cataatggca acacgtttgt cattgtatcc cctcctccca acgtcactgg atatttgcat cttggccact ccttgacagg tgctgtgcaa gatacactta ttcgattcca tcgcatgaag ggcgacaaca ctctttatgt tcctggtaca gaccacgccg gtattgccac acaagtggtt gtggagaaac gtctcttgcg agaatccggt aagacgcgtc atgatcttgg tcgggaagag tttttgaagc gtgtgtggga tttcaaagaa aaccatgcac acgtgattac ccgtcaactt cgacaaattg gtctcagtct ggattggaca cgagagcgct ttacgatgga tgaccattgc gcaggtgcgg tgctggaggc gtttgtgaag ttgcatgaag atggcctggt gcgccgctcc actcgtcttg tgaattggtg ttgtgcactg caaagcgcca tctccgactt ggaggtggaa tttgccgatg tgccaaaaaa tgcaaaaatt gccataccag gatacgacaa gaaggtcgat atgggtgttt tgacgcacgt tgcctacagg tttgaggata gcgatgatga gattgttatt gccacaactc gaccggagac tattctgggt gacacggcgg tggcagtcca cccggatgat gaacggtata agaaatatca tggcaaacgc ctgaagtgcc ctttccgtga tgagaccatt cctttgattc tcgaccctgt attggttgac gtgagttttg gcacaggtgc cgtaaaaatt actccctccc acgatccaaa tgactttgag gcgggggtac gtcacaactt gccacaactg acaatgatgg atctcaatgg acggattacc attgatggcc cttttaaagg aatgcatcgt tttgattgcc gacgtgaaat tgtgaaggaa ctggagaaga tggggcttct gcgtgatgtg gtcccgtacg agtaccgtgt tggtcgctgt agtcgtacag gtgacattgt ggagccactt ttgatgccac aatggtttgt tgactgcagc gaaatggccc gtaaatctgt ggaggcggta cgtaataaag agctgcgtct ttacccttct tcgcacgaag cggtttggtt tcactggttg gagaatataa agccgtggtg cgtctctcga cagctgtggt ggggacaccg tatccctgcg tacaaatgct cgggtgtggt gccatccaac cacgaagatc catgggtggt ggctcgtaac attgcggagg caaggtccaa ggcacgtgcg aaatttaact tgaccgacgc ggaggtggaa caactggtgc tggaccagga tccagatgtg cttgatacgt ggtttagttc tgccatgtgg ccttactcta cccttggctg gccaggggac acagacgata tgcacaagtt cttccctggt aacctaatgg agaccggtca cgacattctc tttttttggg ttgcacgcat ggtgatgacg tcacttcatt tcacaggaaa gttgccattt tcagaagtgt ttttgcacgc tatggtgcgt gacaaaaatg gtgagaagat gagcaaatcc aaagggaatg tgattgaccc actttttatt attaatggta tcactcttga ggggctgcac tccacggtga cgaatggtaa tttggatgag aaggaagttc cccgggcgtt aaagttgcag aaggagacgt tcccgaatgg cattcctgaa tgcggtagcg atgcactccg gtttggcctt ctttcatata ctcaatcagg aagaagcgtc aatcttgaca ttgatcgcat cgtcgcctac cgccaatttt gcaacaaatt atggaatgca gtgcggtacg tcctctacca cgcattggga acggaataca aaccgggcct gacagtgatc gacgttttaa gggcggagag ttatccgctg gaatgccgtt ggattctctc ccgtcttgat gtggcggtgg aggaatgtac ccgcgggttt tcagagggct cctatgactt tgcccttacc actaacgcag cctaccgctt ctggttgtat gagctgtgcg atgtcttcct tgagctgacg aagttttcca ttcatgctgg tggtgataga aagcagctgg tgcaggatgt attattacat gtggtggaag tcgcactccg tctgcttcac cccatgatgc cttttctcac agaagagctc tggcatcgcc taccaaatta caatactttt ggggtggaga gtatcatgct cgccccgtac cccgcagtgg ctggatggaa gaatgacacg gtggaggaga agatgaggat tatgatggat accgtacgca atgtccgttc caccaaagcc tcatattctc ttacaaacaa gcataaaccg gatgtgtgga ttaccgcaca aaccgcagag gtgaagcagt tgcttatgga tcacagctac atggtgattt ccctcggtgt ggtgggaaat gtttccgtca tttcgccgga ggaagtggag gccaatgttc caaagggctg cgggttttct gttgtgaaca aggaggtggg cgtcaacatg atgcttttgg gttttattga cgtacaaaag gaggttgcga agctcgacaa gcagcttgta ggacttcaaa agcagattga aggcctaaag aaaaagatgt ccattccaaa ctacgacatg aaagtacctg cggatgtgcg gactgcgaac agtgagaagc ttgacacgct cacggcacag gagaagcaaa ttctagaagg cctggaaaaa atgaagagca ttctttag
Details for TrcrB.18849.c.B1.GE42709
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 112kDa
OBSERVED MW: 112kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK RLTPQMAAAY TPKDVESEWY EWWEKSGFFR PASDLGRKHN GNTFVIVSPP PNVTGYLHLG HSLTGAVQDT LIRFHRMKGD NTLYVPGTDH AGIATQVVVE KRLLRESGKT RHDLGREEFL KRVWDFKENH AHVITRQLRQ IGLSLDWTRE RFTMDDHCAG AVLEAFVKLH EDGLVRRSTR LVNWCCALQS AISDLEVEFA DVPKNAKIAI PGYDKKVDMG VLTHVAYRFE DSDDEIVIAT TRPETILGDT AVAVHPDDER YKKYHGKRLK CPFRDETIPL ILDPVLVDVS FGTGAVKITP SHDPNDFEAG VRHNLPQLTM MDLNGRITID GPFKGMHRFD CRREIVKELE KMGLLRDVVP YEYRVGRCSR TGDIVEPLLM PQWFVDCSEM ARKSVEAVRN KELRLYPSSH EAVWFHWLEN IKPWCVSRQL WWGHRIPAYK CSGVVPSNHE DPWVVARNIA EARSKARAKF NLTDAEVEQL VLDQDPDVLD TWFSSAMWPY STLGWPGDTD DMHKFFPGNL METGHDILFF WVARMVMTSL HFTGKLPFSE VFLHAMVRDK NGEKMSKSKG NVIDPLFIIN GITLEGLHST VTNGNLDEKE VPRALKLQKE TFPNGIPECG SDALRFGLLS YTQSGRSVNL DIDRIVAYRQ FCNKLWNAVR YVLYHALGTE YKPGLTVIDV LRAESYPLEC RWILSRLDVA VEECTRGFSE GSYDFALTTN AAYRFWLYEL CDVFLELTKF SIHAGGDRKQ LVQDVLLHVV EVALRLLHPM MPFLTEELWH RLPNYNTFGV ESIMLAPYPA VAGWKNDTVE EKMRIMMDTV RNVRSTKASY SLTNKHKPDV WITAQTAEVK QLLMDHSYMV ISLGVVGNVS VISPEEVEAN VPKGCGFSVV NKEVGVNMML LGFIDVQKEV AKLDKQLVGL QKQIEGLKKK MSIPNYDMKV PADVRTANSE KLDTLTAQEK QILEGLEKMK SIL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc ggctgacccc gcagatggct gcggcctata cccccaagga tgtggagagt gaatggtatg agtggtggga aaagtccggc ttcttccgcc ctgcatcgga tttgggccgc aagcataatg gcaacacgtt tgtcattgta tcccctcctc ccaacgtcac tggatatttg catcttggcc actccttgac aggtgctgtg caagatacac ttattcgatt ccatcgcatg aagggcgaca acactcttta tgttcctggt acagaccacg ccggtattgc cacacaagtg gttgtggaga aacgtctctt gcgagaatcc ggtaagacgc gtcatgatct tggtcgggaa gagtttttga agcgtgtgtg ggatttcaaa gaaaaccatg cacacgtgat tacccgtcaa cttcgacaaa ttggtctcag tctggattgg acacgagagc gctttacgat ggatgaccat tgcgcaggtg cggtgctgga ggcgtttgtg aagttgcatg aagatggcct ggtgcgccgc tccactcgtc ttgtgaattg gtgttgtgca ctgcaaagcg ccatctccga cttggaggtg gaatttgccg atgtgccaaa aaatgcaaaa attgccatac caggatacga caagaaggtc gatatgggtg ttttgacgca cgttgcctac aggtttgagg atagcgatga tgagattgtt attgccacaa ctcgaccgga gactattctg ggtgacacgg cggtggcagt ccacccggat gatgaacggt ataagaaata tcatggcaaa cgcctgaagt gccctttccg tgatgagacc attcctttga ttctcgaccc tgtattggtt gacgtgagtt ttggcacagg tgccgtaaaa attactccct cccacgatcc aaatgacttt gaggcggggg tacgtcacaa cttgccacaa ctgacaatga tggatctcaa tggacggatt accattgatg gcccttttaa aggaatgcat cgttttgatt gccgacgtga aattgtgaag gaactggaga agatggggct tctgcgtgat gtggtcccgt acgagtaccg tgttggtcgc tgtagtcgta caggtgacat tgtggagcca cttttgatgc cacaatggtt tgttgactgc agcgaaatgg cccgtaaatc tgtggaggcg gtacgtaata aagagctgcg tctttaccct tcttcgcacg aagcggtttg gtttcactgg ttggagaata taaagccgtg gtgcgtctct cgacagctgt ggtggggaca ccgtatccct gcgtacaaat gctcgggtgt ggtgccatcc aaccacgaag atccatgggt ggtggctcgt aacattgcgg aggcaaggtc caaggcacgt gcgaaattta acttgaccga cgcggaggtg gaacaactgg tgctggacca ggatccagat gtgcttgata cgtggtttag ttctgccatg tggccttact ctacccttgg ctggccaggg gacacagacg atatgcacaa gttcttccct ggtaacctaa tggagaccgg tcacgacatt ctcttttttt gggttgcacg catggtgatg acgtcacttc atttcacagg aaagttgcca ttttcagaag tgtttttgca cgctatggtg cgtgacaaaa atggtgagaa gatgagcaaa tccaaaggga atgtgattga cccacttttt attattaatg gtatcactct tgaggggctg cactccacgg tgacgaatgg taatttggat gagaaggaag ttccccgggc gttaaagttg cagaaggaga cgttcccgaa tggcattcct gaatgcggta gcgatgcact ccggtttggc cttctttcat atactcaatc aggaagaagc gtcaatcttg acattgatcg catcgtcgcc taccgccaat tttgcaacaa attatggaat gcagtgcggt acgtcctcta ccacgcattg ggaacggaat acaaaccggg cctgacagtg atcgacgttt taagggcgga gagttatccg ctggaatgcc gttggattct ctcccgtctt gatgtggcgg tggaggaatg tacccgcggg ttttcagagg gctcctatga ctttgccctt accactaacg cagcctaccg cttctggttg tatgagctgt gcgatgtctt ccttgagctg acgaagtttt ccattcatgc tggtggtgat agaaagcagc tggtgcagga tgtattatta catgtggtgg aagtcgcact ccgtctgctt caccccatga tgccttttct cacagaagag ctctggcatc gcctaccaaa ttacaatact tttggggtgg agagtatcat gctcgccccg taccccgcag tggctggatg gaagaatgac acggtggagg agaagatgag gattatgatg gataccgtac gcaatgtccg ttccaccaaa gcctcatatt ctcttacaaa caagcataaa ccggatgtgt ggattaccgc acaaaccgca gaggtgaagc agttgcttat ggatcacagc tacatggtga tttccctcgg tgtggtggga aatgtttccg tcatttcgcc ggaggaagtg gaggccaatg ttccaaaggg ctgcgggttt tctgttgtga acaaggaggt gggcgtcaac atgatgcttt tgggttttat tgacgtacaa aaggaggttg cgaagctcga caagcagctt gtaggacttc aaaagcagat tgaaggccta aagaaaaaga tgtccattcc aaactacgac atgaaagtac ctgcggatgt gcggactgcg aacagtgaga agcttgacac gctcacggca caggagaagc aaattctaga aggcctggaa aaaatgaaga gcattctttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for TrcrB.18849.c.B1.PW38558
PURIFICATION DATe: 10/30/2018
CONCENTRATION: 10.5mg/ml
OBSERVED MW: 112kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 105µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK RLTPQMAAAY TPKDVESEWY EWWEKSGFFR PASDLGRKHN GNTFVIVSPP PNVTGYLHLG HSLTGAVQDT LIRFHRMKGD NTLYVPGTDH AGIATQVVVE KRLLRESGKT RHDLGREEFL KRVWDFKENH AHVITRQLRQ IGLSLDWTRE RFTMDDHCAG AVLEAFVKLH EDGLVRRSTR LVNWCCALQS AISDLEVEFA DVPKNAKIAI PGYDKKVDMG VLTHVAYRFE DSDDEIVIAT TRPETILGDT AVAVHPDDER YKKYHGKRLK CPFRDETIPL ILDPVLVDVS FGTGAVKITP SHDPNDFEAG VRHNLPQLTM MDLNGRITID GPFKGMHRFD CRREIVKELE KMGLLRDVVP YEYRVGRCSR TGDIVEPLLM PQWFVDCSEM ARKSVEAVRN KELRLYPSSH EAVWFHWLEN IKPWCVSRQL WWGHRIPAYK CSGVVPSNHE DPWVVARNIA EARSKARAKF NLTDAEVEQL VLDQDPDVLD TWFSSAMWPY STLGWPGDTD DMHKFFPGNL METGHDILFF WVARMVMTSL HFTGKLPFSE VFLHAMVRDK NGEKMSKSKG NVIDPLFIIN GITLEGLHST VTNGNLDEKE VPRALKLQKE TFPNGIPECG SDALRFGLLS YTQSGRSVNL DIDRIVAYRQ FCNKLWNAVR YVLYHALGTE YKPGLTVIDV LRAESYPLEC RWILSRLDVA VEECTRGFSE GSYDFALTTN AAYRFWLYEL CDVFLELTKF SIHAGGDRKQ LVQDVLLHVV EVALRLLHPM MPFLTEELWH RLPNYNTFGV ESIMLAPYPA VAGWKNDTVE EKMRIMMDTV RNVRSTKASY SLTNKHKPDV WITAQTAEVK QLLMDHSYMV ISLGVVGNVS VISPEEVEAN VPKGCGFSVV NKEVGVNMML LGFIDVQKEV AKLDKQLVGL QKQIEGLKKK MSIPNYDMKV PADVRTANSE KLDTLTAQEK QILEGLEKMK SIL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC GGCTGACCCC GCAGATGGCT GCGGCCTATA CCCCCAAGGA TGTGGAGAGT GAATGGTATG AGTGGTGGGA AAAGTCCGGC TTCTTCCGCC CTGCATCGGA TTTGGGCCGC AAGCATAATG GCAACACGTT TGTCATTGTA TCCCCTCCTC CCAACGTCAC TGGATATTTG CATCTTGGCC ACTCCTTGAC AGGTGCTGTG CAAGATACAC TTATTCGATT CCATCGCATG AAGGGCGACA ACACTCTTTA TGTTCCTGGT ACAGACCACG CCGGTATTGC CACACAAGTG GTTGTGGAGA AACGTCTCTT GCGAGAATCC GGTAAGACGC GTCATGATCT TGGTCGGGAA GAGTTTTTGA AGCGTGTGTG GGATTTCAAA GAAAACCATG CACACGTGAT TACCCGTCAA CTTCGACAAA TTGGTCTCAG TCTGGATTGG ACACGAGAGC GCTTTACGAT GGATGACCAT TGCGCAGGTG CGGTGCTGGA GGCGTTTGTG AAGTTGCATG AAGATGGCCT GGTGCGCCGC TCCACTCGTC TTGTGAATTG GTGTTGTGCA CTGCAAAGCG CCATCTCCGA CTTGGAGGTG GAATTTGCCG ATGTGCCAAA AAATGCAAAA ATTGCCATAC CAGGATACGA CAAGAAGGTC GATATGGGTG TTTTGACGCA CGTTGCCTAC AGGTTTGAGG ATAGCGATGA TGAGATTGTT ATTGCCACAA CTCGACCGGA GACTATTCTG GGTGACACGG CGGTGGCAGT CCACCCGGAT GATGAACGGT ATAAGAAATA TCATGGCAAA CGCCTGAAGT GCCCTTTCCG TGATGAGACC ATTCCTTTGA TTCTCGACCC TGTATTGGTT GACGTGAGTT TTGGCACAGG TGCCGTAAAA ATTACTCCCT CCCACGATCC AAATGACTTT GAGGCGGGGG TACGTCACAA CTTGCCACAA CTGACAATGA TGGATCTCAA TGGACGGATT ACCATTGATG GCCCTTTTAA AGGAATGCAT CGTTTTGATT GCCGACGTGA AATTGTGAAG GAACTGGAGA AGATGGGGCT TCTGCGTGAT GTGGTCCCGT ACGAGTACCG TGTTGGTCGC TGTAGTCGTA CAGGTGACAT TGTGGAGCCA CTTTTGATGC CACAATGGTT TGTTGACTGC AGCGAAATGG CCCGTAAATC TGTGGAGGCG GTACGTAATA AAGAGCTGCG TCTTTACCCT TCTTCGCACG AAGCGGTTTG GTTTCACTGG TTGGAGAATA TAAAGCCGTG GTGCGTCTCT CGACAGCTGT GGTGGGGACA CCGTATCCCT GCGTACAAAT GCTCGGGTGT GGTGCCATCC AACCACGAAG ATCCATGGGT GGTGGCTCGT AACATTGCGG AGGCAAGGTC CAAGGCACGT GCGAAATTTA ACTTGACCGA CGCGGAGGTG GAACAACTGG TGCTGGACCA GGATCCAGAT GTGCTTGATA CGTGGTTTAG TTCTGCCATG TGGCCTTACT CTACCCTTGG CTGGCCAGGG GACACAGACG ATATGCACAA GTTCTTCCCT GGTAACCTAA TGGAGACCGG TCACGACATT CTCTTTTTTT GGGTTGCACG CATGGTGATG ACGTCACTTC ATTTCACAGG AAAGTTGCCA TTTTCAGAAG TGTTTTTGCA CGCTATGGTG CGTGACAAAA ATGGTGAGAA GATGAGCAAA TCCAAAGGGA ATGTGATTGA CCCACTTTTT ATTATTAATG GTATCACTCT TGAGGGGCTG CACTCCACGG TGACGAATGG TAATTTGGAT GAGAAGGAAG TTCCCCGGGC GTTAAAGTTG CAGAAGGAGA CGTTCCCGAA TGGCATTCCT GAATGCGGTA GCGATGCACT CCGGTTTGGC CTTCTTTCAT ATACTCAATC AGGAAGAAGC GTCAATCTTG ACATTGATCG CATCGTCGCC TACCGCCAAT TTTGCAACAA ATTATGGAAT GCAGTGCGGT ACGTCCTCTA CCACGCATTG GGAACGGAAT ACAAACCGGG CCTGACAGTG ATCGACGTTT TAAGGGCGGA GAGTTATCCG CTGGAATGCC GTTGGATTCT CTCCCGTCTT GATGTGGCGG TGGAGGAATG TACCCGCGGG TTTTCAGAGG GCTCCTATGA CTTTGCCCTT ACCACTAACG CAGCCTACCG CTTCTGGTTG TATGAGCTGT GCGATGTCTT CCTTGAGCTG ACGAAGTTTT CCATTCATGC TGGTGGTGAT AGAAAGCAGC TGGTGCAGGA TGTATTATTA CATGTGGTGG AAGTCGCACT CCGTCTGCTT CACCCCATGA TGCCTTTTCT CACAGAAGAG CTCTGGCATC GCCTACCAAA TTACAATACT TTTGGGGTGG AGAGTATCAT GCTCGCCCCG TACCCCGCAG TGGCTGGATG GAAGAATGAC ACGGTGGAGG AGAAGATGAG GATTATGATG GATACCGTAC GCAATGTCCG TTCCACCAAA GCCTCATATT CTCTTACAAA CAAGCATAAA CCGGATGTGT GGATTACCGC ACAAACCGCA GAGGTGAAGC AGTTGCTTAT GGATCACAGC TACATGGTGA TTTCCCTCGG TGTGGTGGGA AATGTTTCCG TCATTTCGCC GGAGGAAGTG GAGGCCAATG TTCCAAAGGG CTGCGGGTTT TCTGTTGTGA ACAAGGAGGT GGGCGTCAAC ATGATGCTTT TGGGTTTTAT TGACGTACAA AAGGAGGTTG CGAAGCTCGA CAAGCAGCTT GTAGGACTTC AAAAGCAGAT TGAAGGCCTA AAGAAAAAGA TGTCCATTCC AAACTACGAC ATGAAAGTAC CTGCGGATGT GCGGACTGCG AACAGTGAGA AGCTTGACAC GCTCACGGCA CAGGAGAAGC AAATTCTAGA AGGCCTGGAA AAAATGAAGA GCATTCTTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT