SSGCID: Target Page

TrcrB.18849.b

valyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18849.b
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18849.b.B1.GE42708	full length		1	413

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.510443.9
UniProt:	Q4CPJ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKRLTPQMAA AYTPKDVESE WYEWWEKSGF FRPASDLGRK HNGNTFVIVS PPPNVTGYLH LGHSLTGAVQ DTLIRFHRMK GDNTLYVPGT DHAGIATQVV VEKRLLRESG KTRHDLGREE FLKRVWDFKE NHAHVITRQL RQIGLSLDWT RERFTMDDHC AGAVLEAFVK MHEDGLVHRS TRLVNWCCAL QSAISDLEVE FADVPKNAKI AIPGYDKKVD MGVLTHVAYR FEDSDDEIVI ATTRPETILG DTAVAVHPDD ERYKKYHGKR LKCPFRDETI PLILDPVLVD VSFGTGAVKI TPSHDPNDFE AGVRHNLPQL TMMDLNGRIT IDGPFKGMHR FDCRREIVKE LEKMGLLRDV VPYEYRVGRC SRTGDIVEPL LMPQWFVDCS EMARKSVEAV RNKELRLYPS SHE

NT Sequence

atgaagcggc tgaccccgca gatggctgcg gcctataccc ccaaggatgt ggagagtgaa tggtatgagt ggtgggaaaa gtctggcttc ttccgccctg catcggattt gggccgcaag cataatggca acacgtttgt cattgtatcc cctcctccca acgtcactgg atatttgcat cttggccact ccttgacagg tgctgtgcaa gatacactta ttcgattcca tcgcatgaag ggcgacaaca ctctttatgt tcctggcaca gaccacgccg gtattgccac acaagtggtt gtggagaaac gtctcttgcg agaatccggt aagacgcgtc atgatcttgg tcgggaagag tttttgaagc gtgtgtggga tttcaaagaa aaccatgcac acgtgattac ccgtcaactt cgacaaattg gtctcagttt ggattggaca cgagagcgct ttacgatgga tgaccattgc gcaggtgcgg tgctggaggc gtttgtgaag atgcatgagg atggtctggt gcaccgctcc actcgtcttg tgaattggtg ttgtgcactg caaagcgcca tctccgactt ggaggtggaa tttgccgatg tgccaaaaaa tgcaaaaatt gccatcccag gatacgacaa gaaggtcgat atgggtgttt tgacgcacgt tgcctacagg tttgaggata gcgatgatga gattgttatc gccacaactc gaccggagac tattctgggt gacacggcgg tggcagtcca cccggatgat gaacggtata agaaatatca tggcaaacgc ctgaagtgcc ctttccgtga tgagaccatt cctttgattc tcgaccctgt attggttgac gtgagttttg gcacaggtgc cgtaaaaatt actccctccc acgatccaaa tgactttgag gcgggggtac gtcacaactt gccacagttg acaatgatgg atctcaatgg acggattacc attgatggcc cttttaaagg aatgcatcgt tttgattgcc gacgtgaaat tgtgaaggaa ctggagaaga tggggcttct gcgtgatgtg gtcccgtacg agtaccgtgt tggtcgctgt agtcgtacag gtgacattgt ggagccactt ttgatgccac aatggtttgt tgactgcagc gaaatggccc gtaaatctgt ggaggcggta cgtaataaag agctgcgtct ttacccttct tcgcacgaa

Details for TrcrB.18849.b.B1.GE42708

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMK RLTPQMAAAY TPKDVESEWY EWWEKSGFFR PASDLGRKHN GNTFVIVSPP PNVTGYLHLG HSLTGAVQDT LIRFHRMKGD NTLYVPGTDH AGIATQVVVE KRLLRESGKT RHDLGREEFL KRVWDFKENH AHVITRQLRQ IGLSLDWTRE RFTMDDHCAG AVLEAFVKMH EDGLVHRSTR LVNWCCALQS AISDLEVEFA DVPKNAKIAI PGYDKKVDMG VLTHVAYRFE DSDDEIVIAT TRPETILGDT AVAVHPDDER YKKYHGKRLK CPFRDETIPL ILDPVLVDVS FGTGAVKITP SHDPNDFEAG VRHNLPQLTM MDLNGRITID GPFKGMHRFD CRREIVKELE KMGLLRDVVP YEYRVGRCSR TGDIVEPLLM PQWFVDCSEM ARKSVEAVRN KE

Validated NT Sequence

cagctcttta ttacgtaccg cctccacaga tttacgggcc atttcgctgc agtcaacaaa ccattgtggc atcaaaagtg gctccacaat gtcacctgta cgactacagc gaccaacacg gtactcgtac gggaccacat cacgcagaag ccccatcttc tccagttcct tcacaatttc acgtcggcaa tcaaaacgat gcattccttt aaaagggcca tcaatggtaa tccgtccatt gagatccatc attgtcaact gtggcaagtt gtgacgtacc cccgcctcaa agtcatttgg atcgtgggag ggagtaattt ttacggcacc tgtgccaaaa ctcacgtcaa ccaatacagg gtcgagaatc aaaggaatgg tctcatcacg gaaagggcac ttcaggcgtt tgccatgata tttcttatac cgttcatcat ccgggtggac tgccaccgcc gtgtcaccca gaatagtctc cggtcgagtt gtggcgataa caatctcatc atcgctatcc tcaaacctgt aggcaacgtg cgtcaaaaca cccatatcga ccttcttgtc gtatcctggg atggcaattt ttgcattttt tggcacatcg gcaaattcca cctccaagtc ggagatggcg ctttgcagtg cacaacacca attcacaaga cgagtggagc ggtgcaccag accatcctca tgcatcttca caaacgcctc cagcaccgca cctgcgcaat ggtcatccat cgtaaagcgc tctcgtgtcc aatccaaact gagaccaatt tgtcgaagtt gacgggtaat cacgtgtgca tggttttctt tgaaatccca cacacgcttc aaaaactctt cccgaccaag atcatgacgc gtcttaccgg attctcgcaa gagacgtttc tccacaacca cttgtgtggc aataccggcg tggtctgtgc caggaacata aagagtgttg tcgcccttca tgcgatggaa tcgaataagt gtatcttgca cagcacctgt caaggagtgg ccaagatgca aatatccagt gacgttggga ggaggggata caatgacaaa cgtgttgcca ttatgcttgc ggcccaaatc cgatgcaggg cggaagaagc cagacttttc ccaccactca taccattcac tctccacatc cttgggggta taggccgcag ccatctgcgg ggtcagccgc ttcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc ggctgacccc gcagatggct gcggcctata cccccaagga tgtggagagt gaatggtatg agtggtggga aaagtctggc ttcttccgcc ctgcatcgga tttgggccgc aagcataatg gcaacacgtt tgtcattgta tcccctcctc ccaacgtcac tggatatttg catcttggcc actccttgac aggtgctgtg caagatacac ttattcgatt ccatcgcatg aagggcgaca acactcttta tgttcctggc acagaccacg ccggtattgc cacacaagtg gttgtggaga aacgtctctt gcgagaatcc ggtaagacgc gtcatgatct tggtcgggaa gagtttttga agcgtgtgtg ggatttcaaa gaaaaccatg cacacgtgat tacccgtcaa cttcgacaaa ttggtctcag tttggattgg acacgagagc gctttacgat ggatgaccat tgcgcaggtg cggtgctgga ggcgtttgtg aagatgcatg aggatggtct ggtgcaccgc tccactcgtc ttgtgaattg gtgttgtgca ctgcaaagcg ccatctccga cttggaggtg gaatttgccg atgtgccaaa aaatgcaaaa attgccatcc caggatacga caagaaggtc gatatgggtg ttttgacgca cgttgcctac aggtttgagg atagcgatga tgagattgtt atcgccacaa ctcgaccgga gactattctg ggtgacacgg cggtggcagt ccacccggat gatgaacggt ataagaaata tcatggcaaa cgcctgaagt gccctttccg tgatgagacc attcctttga ttctcgaccc tgtattggtt gacgtgagtt ttggcacagg tgccgtaaaa attactccct cccacgatcc aaatgacttt gaggcggggg tacgtcacaa cttgccacag ttgacaatga tggatctcaa tggacggatt accattgatg gcccttttaa aggaatgcat cgttttgatt gccgacgtga aattgtgaag gaactggaga agatggggct tctgcgtgat gtggtcccgt acgagtaccg tgttggtcgc tgtagtcgta caggtgacat tgtggagcca cttttgatgc cacaatggtt tgttgactgc agcgaaatgg cccgtaaatc tgtggaggcg gtacgtaata aagagctgcg tctttaccct tcttcgcacg aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat