TrcrB.18849.a

valyl-tRNA synthetase, putative

CENTER ID: TrcrB.18849.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18849.a.B1.GE42707 full length 1 404
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.504257.70
UniProt: Q4CWP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
GNVIDPLFII NGITLEGLHS TVTNGNLDEK EVPRALKLQK ETFPNGIPEC GSDALRFGLL SYTQSGRSVN LDIDRIVAYR QFCNKLWNAV RYVLYHALGT EYKPGLTVID VSRAESYPLE CRWILSRLDV AVEECTRGFS EGSYDFALTT NSAYRFWLYE LCDVFLELTK FSIHAGGDRK QLVQDVLLYV VEVALRLLHP MMPFLTEELW HRLPNYNTFE VESIMLAPYP AVAGWKNDTV EEKMRIMMDT VHNVRSTKAS YSLTNKHKPD VWITAQTSEV KQLLMDHSYM VISLGVVGNV SVISPEEVEA NVPKGCGFSV VNKEVGVNMM LLGFIDVQKE VAKLDKQLVG LQKQIEGLKK KMSIPNYDMK VPADVRTANS EKLDTLTAQE KQIIEGLEKM RSIL
NT Sequence
gggaatgtga ttgacccact ttttattatt aatggtatca ctcttgaggg gctgcactcc acggtgacga atggtaattt ggatgagaag gaagttcccc gggcgttaaa gttgcagaag gagacgttcc cgaatggcat tcctgaatgc ggtagcgatg cactccggtt tggccttctt tcatatactc aatcaggaag aagcgtcaat cttgacattg atcgcatcgt cgcctaccgc caattttgca acaaattatg gaatgcggtg cggtacgtcc tctaccacgc attgggaacg gaatacaaac cgggcctgac agtgatcgac gtttcaaggg cggagagtta tccactggaa tgccgttgga ttctctcccg tcttgatgtg gcggtggagg aatgtacccg cgggttttca gagggctctt atgactttgc ccttaccact aactcagcct accgcttctg gttgtatgag ctgtgcgatg tcttccttga gctgacgaag ttttccattc atgctggtgg tgatagaaag cagctggtgc aggatgtatt attatatgtg gtggaagtcg cgctacgtct gcttcacccc atgatgcctt ttctcacaga agagctctgg catcgcctac caaattacaa tacttttgag gtggagagta tcatgctcgc cccgtacccc gcagtggctg gatggaagaa tgacacggtg gaggagaaaa tgaggattat gatggatacc gtacacaatg tccgttccac caaagcctca tattctctta caaacaagca caaaccggat gtgtggatta ccgcacaaac ctcagaggtg aagcagttgc ttatggatca cagctacatg gtgatttccc tcggtgtggt gggaaatgtt tccgtcattt cgccggagga agtggaagcc aatgttccaa agggctgcgg gttttctgtt gtgaacaagg aggtgggcgt caacatgatg ctcttgggtt ttattgacgt gcagaaggag gttgcgaagc tcgacaagca gcttgtagga cttcaaaagc agattgaagg cctaaagaaa aagatgtcca ttccaaacta cgacatgaaa gtacctgcgg atgtgcggac tgcgaacagt gagaagcttg acacgctcac ggcacaggag aagcaaatta tagaaggcct ggaaaaaatg aggagcattc tttag
Details for TrcrB.18849.a.B1.GE42707
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG NVIDPLFIIN GITLEGLHST VTNGNLDEKE VPRALKLQKE TFPNGIPECG SDALRFGLLS YTQSGRSVNL DIDRIVAYRQ FCNKLWNAVR YVLYHALGTE YKPGLTVIDV SRAESYPLEC RWILSRLDVA VEECTRGFSE GSYDFALTTN SAYRFWLYEL CDVFLELTKF SIHAGGDRKQ LVQDVLLYVV EVALRLLHPM MPFLTEELWH RLPNYNTFEV ESIMLAPYPA VAGWKNDTVE EKMRIMMDTV HNVRSTKASY SLTNKHKPDV WITAQTSEVK QLLMDHSYMV ISLGVVGNVS VISPEEVEAN VPKGCGFSVV NKEVGVNMML LGFIDVQKEV AKLDKQLVGL QKQIEGLKKK MSIPNYDMKV PADVRTANSE KLDTLTAQEK QIIE
Validated NT Sequence
gccttctata atttgcttct cctgtgccgt gagcgtgtca agcttctcac tgttcgcagt ccgcacatcc gcaggtactt tcatgtcgta gtttggaatg gacatctttt tctttaggcc ttcaatctgc ttttgaagtc ctacaagctg cttgtcgagc ttcgcaacct ccttctgcac gtcaataaaa cccaagagca tcatgttgac gcccacctcc ttgttcacaa cagaaaaccc gcagcccttt ggaacattgg cttccacttc ctccggcgaa atgacggaaa catttcccac cacaccgagg gaaatcacca tgtagctgtg atccataagc aactgcttca cctctgaggt ttgtgcggta atccacacat ccggtttgtg cttgtttgta agagaatatg aggctttggt ggaacggaca ttgtgtacgg tatccatcat aatcctcatt ttctcctcca ccgtgtcatt cttccatcca gccactgcgg ggtacggggc gagcatgata ctctccacct caaaagtatt gtaatttggt aggcgatgcc agagctcttc tgtgagaaaa ggcatcatgg ggtgaagcag acgtagcgcg acttccacca catataataa tacatcctgc accagctgct ttctatcacc accagcatga atggaaaact tcgtcagctc aaggaagaca tcgcacagct catacaacca gaagcggtag gctgagttag tggtaagggc aaagtcataa gagccctctg aaaacccgcg ggtacattcc tccaccgcca catcaagacg ggagagaatc caacggcatt ccagtggata actctccgcc cttgaaacgt cgatcactgt caggcccggt ttgtattccg ttcccaatgc gtggtagagg acgtaccgca ccgcattcca taatttgttg caaaattggc ggtaggcgac gatgcgatca atgtcaagat tgacgcttct tcctgattga gtatatgaaa gaaggccaaa ccggagtgca tcgctaccgc attcaggaat gccattcggg aacgtctcct tctgcaactt taacgcccgg ggaacttcct tctcatccaa attaccattc gtcaccgtgg agtgcagccc ctcaagagtg ataccattaa taataaaaag tgggtcaatc acattcccca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHGN VIDPLFIING ITLEGLHSTV TNGNLDEKEV PRALKLQKET FPNGIPECGS DALRFGLLSY TQSGRSVNLD IDRIVAYRQF CNKLWNAVRY VLYHALGTEY KPGLTVIDVS RAESYPLECR WILSRLDVAV EECTRGFSEG SYDFALTTNS AYRFWLYELC DVFLELTKFS IHAGGDRKQL VQDVLLYVVE VALRLLHPMM PFLTEELWHR LPNYNTFEVE SIMLAPYPAV AGWKNDTVEE KMRIMMDTVH NVRSTKASYS LTNKHKPDVW ITAQTSEVKQ LLMDHSYMVI SLGVVGNVSV ISPEEVEANV PKGCGFSVVN KEVGVNMMLL GFIDVQKEVA KLDKQLVGLQ KQIEGLKKKM SIPNYDMKVP ADVRTANSEK LDTLTAQEKQ IIEGLEKMRS IL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggaatg tgattgaccc actttttatt attaatggta tcactcttga ggggctgcac tccacggtga cgaatggtaa tttggatgag aaggaagttc cccgggcgtt aaagttgcag aaggagacgt tcccgaatgg cattcctgaa tgcggtagcg atgcactccg gtttggcctt ctttcatata ctcaatcagg aagaagcgtc aatcttgaca ttgatcgcat cgtcgcctac cgccaatttt gcaacaaatt atggaatgcg gtgcggtacg tcctctacca cgcattggga acggaataca aaccgggcct gacagtgatc gacgtttcaa gggcggagag ttatccactg gaatgccgtt ggattctctc ccgtcttgat gtggcggtgg aggaatgtac ccgcgggttt tcagagggct cttatgactt tgcccttacc actaactcag cctaccgctt ctggttgtat gagctgtgcg atgtcttcct tgagctgacg aagttttcca ttcatgctgg tggtgataga aagcagctgg tgcaggatgt attattatat gtggtggaag tcgcgctacg tctgcttcac cccatgatgc cttttctcac agaagagctc tggcatcgcc taccaaatta caatactttt gaggtggaga gtatcatgct cgccccgtac cccgcagtgg ctggatggaa gaatgacacg gtggaggaga aaatgaggat tatgatggat accgtacaca atgtccgttc caccaaagcc tcatattctc ttacaaacaa gcacaaaccg gatgtgtgga ttaccgcaca aacctcagag gtgaagcagt tgcttatgga tcacagctac atggtgattt ccctcggtgt ggtgggaaat gtttccgtca tttcgccgga ggaagtggaa gccaatgttc caaagggctg cgggttttct gttgtgaaca aggaggtggg cgtcaacatg atgctcttgg gttttattga cgtgcagaag gaggttgcga agctcgacaa gcagcttgta ggacttcaaa agcagattga aggcctaaag aaaaagatgt ccattccaaa ctacgacatg aaagtacctg cggatgtgcg gactgcgaac agtgagaagc ttgacacgct cacggcacag gagaagcaaa ttatagaagg cctggaaaaa atgaggagca ttctttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat