SSGCID: Target Page

TrcrB.18816.a

arginyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18816.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18816.a.B1.GE42706	full length		1	692

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.508355.320
UniProt:	Q4E397

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARICTGANV ELALKDIVRH AMEKAFPTIS TPEVLISLGK VTEYQCNNAM GLAKLLSKAT PPMKMSPINV GEELRKNLEE NDLIERFEPT PQGFINIAIK QEWAATMVSR LLKMGIQPPI IPPKKVLVDF SSPNIAKEMH VGHLRSTIIG ETICRLFEFC GFEVHRINHV GDWGTAFGML ILYLKRQHPN FLTEMPDITD LTKFYREAKK CFDEDPKFKE EARLQVVKLQ ALENESIQAW KIICDISRKE FSLIYDRLGT RIEERGESFY NPIIPRVLSL LQEAGKLEES SGAKLVISKE KKLISSLNAK DMAKLIVPHL IQIWRDNTVE FHVNMITVLR EVGILTGEEG NETIMLSKKE TKPLSKFDAR TDIDKIVPKL EPYYKNQLSP LFREVFEAAG ITDGDTIAVP RFNFPLILQK SDGGFTYDTT DVTSMYHRFV MEKMDRVVYC TDVGQYEHFR MCAQLAKDMG WMGDATWDHA GFGLVTGADG KKIKTRSGET AKLKDLLDEA VERSIAILEE REGGERSQGH TKEEMETLSK IIGIGAIKYF DLKQSRVNDY AFSYDKMLDM SGNTAVFLLY QYARLCSIKR KANVSEDEFM KAEVVINTPQ EKRLALCAFR MESVLLKTVE DLFPHHLTDF AYELVSCFSD FFQNCKVVGD PMQNSRLCLV ELTRITLKKV FDILNIEATE RI

NT Sequence

atggcacgta tatgcacggg ggccaatgtt gagcttgctc tcaaggatat tgtgcgtcat gcgatggaaa aggcattccc caccattagc acaccggagg ttctcatttc actgggtaaa gtcacagagt accagtgcaa caatgcgatg ggattagcca aacttctctc caaggcaaca cctccgatga agatgagtcc tatcaatgtt ggtgaggagt taagaaagaa tcttgaggag aatgatctca ttgaaaggtt tgaaccgaca ccacaggggt tcattaacat cgccatcaaa caggaatggg cagctaccat ggtgtcgcgt ttgcttaaaa tgggcattca gcctcccata atccctccaa aaaaggtgct tgtggacttt tcttcaccca acattgcgaa ggagatgcat gtgggtcatc tccgctccac cattattggc gaaaccattt gccgtctttt tgaattctgt ggctttgagg tgcaccgcat caatcatgtg ggtgattggg gcacggcgtt tggtatgctt atcctgtact tgaaacgtca gcacccgaac tttcttactg aaatgccgga cattacggat cttactaaat tctaccgtga ggccaaaaaa tgctttgatg aggaccccaa gtttaaggag gaggcaagac tgcaggtggt taagctccag gcattggaaa atgaatctat tcaagcgtgg aaaattattt gtgacatttc ccggaaagaa tttagtctca tttatgatcg tctgggcact cgcatcgagg agcgtggaga gagtttctac aatcctatca ttccaagggt gttgagtctg cttcaggaag ctggaaagct agaggaaagc agcggcgcga agcttgtcat tagcaaggag aagaagttga tcagttcact gaatgcgaaa gatatggcca aacttattgt gccacatctt attcaaattt ggcgtgataa cacggtagag ttccacgtga atatgatcac ggtactgagg gaggtgggta tcttgacggg tgaggaaggc aacgaaacaa taatgctttc aaagaaggag accaaaccgc tatcaaagtt tgatgcacgc acagacattg acaagattgt acccaaactt gaaccttatt ataagaatca gttgtcgcca ctttttcgtg aagtttttga ggcggcaggt ataactgatg gggatacaat tgccgtcccg cggttcaatt ttccactcat tcttcagaag agtgacggcg ggttcacata tgataccacc gatgttacgt caatgtatca ccgatttgtc atggagaaaa tggatcgtgt tgtttactgt acagatgtgg gacaatacga gcacttccgt atgtgcgctc agctggcgaa agacatggga tggatggggg atgcgacgtg ggatcatgcc ggttttggtc ttgtcacggg cgcggatggc aaaaagatta agacccgcag tggcgagacg gcaaaactaa aggatttact tgacgaggcg gtggagcgtt ccatagccat tttagaggaa cgtgaaggtg gtgaacgtag tcaggggcat acaaaagaag aaatggaaac cctgtctaaa attattggta tcggtgccat caaatatttt gatttgaagc agagtcgtgt taatgattac gctttcagtt acgacaagat gcttgatatg tcaggtaaca ccgccgtgtt tctcttgtat cagtacgccc gtttatgttc catcaaacgc aaggcaaatg tgagtgaaga tgaattcatg aaggcggaag tagtcatcaa cactccgcag gaaaagcggc tggcgctttg tgccttccgc atggaatctg ttctgttgaa gactgtggag gacctctttc ctcaccatct cacggatttt gcgtatgagt tagtgtcatg cttctctgac ttctttcaga actgcaaggt tgttggggat cccatgcaaa acagccgtct ctgtcttgtg gagctcacac gcatcacatt gaaaaaggtt tttgatatcc ttaacattga ggcgacagaa cggatttga

Details for TrcrB.18816.a.B1.GE42706

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	80kDa
OBSERVED MW:	79kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA RICTGANVEL ALKDIVRHAM EKAFPTISTP EVLISLGKVT EYQCNNAMGL AKLLSKATPP MKMSPINVGE ELRKNLEEND LIERFEPTPQ GFINIAIKQE WAATMVSRLL KMGIQPPIIP PKKVLVDFSS PNIAKEMHVG HLRSTIIGET ICRLFEFCGF EVHRINHVGD WGTAFGMLIL YLKRQHPNFL TEMPDITDLT KFYREAKKCF DEDPKFKEEA RLQVVKLQAL ENESIQAWKI ICDISRKEFS LIYDRLGTRI EERGESFYNP IIPRVLSLLQ EAGKLEESSG AKLVISKEKK LISSLNAKDM AKLIVPHLIQ IWRDNTVEFH VNMITVLREV GILTGEEGNE TIMLSKKETK PLSKFDARTD IDKIVPKLEP YYKNQLSPLF REVFEAAGIT DGDTIAVPRF NFPLILQKSD GGFTYDTTDV TSMYHRFVME KMDRVVYCTD VGQYEHFRMC AQLAKDMGWM GDATWDHAGF GLVTGADGKK IKTRSGETAK LKDLLDEAVE RSIAILEERE GGERSQGHTK EEMETLSKII GIGAIKYFDL KQSRVNDYAF SYDKMLDMSG NTAVFLLYQY ARLCSIKRKA NVSEDEFMKA EVVINTPQEK RLALCAFRME SVLLKTVEDL FPHHLTDFAY ELVSCFSDFF QNCKVVGDPM QNSRLCLVEL TRITLKKVFD ILNIEATERI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac gtatatgcac gggggccaat gttgagcttg ctctcaagga tattgtgcgt catgcgatgg aaaaggcatt ccccaccatt agcacaccgg aggttctcat ttcactgggt aaagtcacag agtaccagtg caacaatgcg atgggattag ccaaacttct ctccaaggca acacctccga tgaagatgag tcctatcaat gttggtgagg agttaagaaa gaatcttgag gagaatgatc tcattgaaag gtttgaaccg acaccacagg ggttcattaa catcgccatc aaacaggaat gggcagctac catggtgtcg cgtttgctta aaatgggcat tcagcctccc ataatccctc caaaaaaggt gcttgtggac ttttcttcac ccaacattgc gaaggagatg catgtgggtc atctccgctc caccattatt ggcgaaacca tttgccgtct ttttgaattc tgtggctttg aggtgcaccg catcaatcat gtgggtgatt ggggcacggc gtttggtatg cttatcctgt acttgaaacg tcagcacccg aactttctta ctgaaatgcc ggacattacg gatcttacta aattctaccg tgaggccaaa aaatgctttg atgaggaccc caagtttaag gaggaggcaa gactgcaggt ggttaagctc caggcattgg aaaatgaatc tattcaagcg tggaaaatta tttgtgacat ttcccggaaa gaatttagtc tcatttatga tcgtctgggc actcgcatcg aggagcgtgg agagagtttc tacaatccta tcattccaag ggtgttgagt ctgcttcagg aagctggaaa gctagaggaa agcagcggcg cgaagcttgt cattagcaag gagaagaagt tgatcagttc actgaatgcg aaagatatgg ccaaacttat tgtgccacat cttattcaaa tttggcgtga taacacggta gagttccacg tgaatatgat cacggtactg agggaggtgg gtatcttgac gggtgaggaa ggcaacgaaa caataatgct ttcaaagaag gagaccaaac cgctatcaaa gtttgatgca cgcacagaca ttgacaagat tgtacccaaa cttgaacctt attataagaa tcagttgtcg ccactttttc gtgaagtttt tgaggcggca ggtataactg atggggatac aattgccgtc ccgcggttca attttccact cattcttcag aagagtgacg gcgggttcac atatgatacc accgatgtta cgtcaatgta tcaccgattt gtcatggaga aaatggatcg tgttgtttac tgtacagatg tgggacaata cgagcacttc cgtatgtgcg ctcagctggc gaaagacatg ggatggatgg gggatgcgac gtgggatcat gccggttttg gtcttgtcac gggcgcggat ggcaaaaaga ttaagacccg cagtggcgag acggcaaaac taaaggattt acttgacgag gcggtggagc gttccatagc cattttagag gaacgtgaag gtggtgaacg tagtcagggg catacaaaag aagaaatgga aaccctgtct aaaattattg gtatcggtgc catcaaatat tttgatttga agcagagtcg tgttaatgat tacgctttca gttacgacaa gatgcttgat atgtcaggta acaccgccgt gtttctcttg tatcagtacg cccgtttatg ttccatcaaa cgcaaggcaa atgtgagtga agatgaattc atgaaggcgg aagtagtcat caacactccg caggaaaagc ggctggcgct ttgtgccttc cgcatggaat ctgttctgtt gaagactgtg gaggacctct ttcctcacca tctcacggat tttgcgtatg agttagtgtc atgcttctct gacttctttc agaactgcaa ggttgttggg gatcccatgc aaaacagccg tctctgtctt gtggagctca cacgcatcac attgaaaaag gtttttgata tccttaacat tgaggcgaca gaacggattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat