SSGCID: Target Page

TrcrB.18809.b

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34)

CENTER ID:	TrcrB.18809.b
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18809.b.B2.GE41050	soluble domain		1	418

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tc00.1047053509167.20
UniProt:	Q4DQR5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCIAKGIFKR SLSGKESMFR RAILLGCSAA KTPWSECSNA QLVDAVKSRK ISFYGLEQAL EPDYRRAIEV RREVVSEIAS QQPEAKKKQS ALHTIPFENY DWNRVVGQNC ENIIGYVPIP LGVAGPILID GKEYPIPMAT TEGALVASTH RGARAITRSG GCKTLLLGEG MTRAPVVELP SLEEAGRLHK YCNENFLSLK EAFESTTQYG KLNSLKCVLA GRKAYLRFRA TTGDAMGMNM ITKGVDKALS VLQQHFPSME ILALSGNYCT DKKPSAVNWI DGRGKSVVAE ATLLAEVVED TLKCTVDSLV SLNIDKNLVG SAMAGSVGGF NAQAANAVAA IFIATGQDPA QVVESSMCIT TMSKVGNDLL ISVTMPSIEV GVVGGGTGLA AQRGCLELIG CGGPSKESPG TNAQLLSRVV AAGVLSAELS LMSGLAAGHL LSAHMRLNRK KK

NT Sequence

atgtgcattg ctaagggaat tttcaaaaga tccctatctg ggaaggaatc gatgtttcgt agggcaattc ttcttggatg cagcgctgcg aaaactccat ggagtgaatg tagtaatgct caacttgtgg atgctgtgaa gtcccgcaaa atttcatttt atggcttgga acaggcattg gagcctgatt acaggcgtgc cattgaggtg cgtcgtgagg tggtctccga aattgcttcc cagcagccag aagccaagaa aaaacagagt gccctgcata ctatcccctt tgagaattat gactggaaca gggttgtcgg tcaaaactgt gaaaatatca taggatatgt accaattccg cttggtgttg ccgggccaat acttattgac ggaaaggagt accctattcc tatggctaca acggaggggg cgcttgtagc tagcacccat cgtggcgctc gagccataac acgatctggc ggttgcaaga cacttttgtt aggtgagggc atgacaaggg ctcccgtggt ggagcttcca tctctggagg aagcgggaag gcttcataag tactgtaatg aaaatttctt atcactaaag gaggcttttg agtccacaac gcagtatggg aagctgaatt cgctaaagtg tgttttggca ggacgaaaag cgtatctccg gttccgtgcg accacgggtg atgcaatggg aatgaacatg atcacaaaag gcgtggataa agccttgtca gtcctgcagc agcattttcc ttcaatggag atccttgctt tatcaggtaa ttattgcaca gacaagaagc catcagcggt aaactggatt gatggacgtg gaaagtcggt agtagcagag gctacattgc ttgctgaagt cgttgaagac accctgaaat gcacagttga cagccttgta tctcttaata ttgataagaa tcttgttggt tcagctatgg ctgggtctgt tggtggattc aacgcacaag ctgcgaatgc cgttgctgcc atttttattg cgacagggca ggacccagcg caggtggtgg agtcttcaat gtgcattacg accatgtcaa aggtgggcaa tgatcttctt atctccgtga caatgccaag cattgaggtt ggtgttgttg gagggggcac aggactggcg gcgcagcgag gctgtttaga gttgattggg tgtggcggcc cgagcaagga atcaccgggt acaaatgcac agttgctttc tcgtgttgtc gctgcgggtg tactttcagc tgagctttct ctcatgtctg gcttggccgc gggtcacctc ctaagcgccc acatgcgcct gaatcgcaag aagaagtga

Details for TrcrB.18809.b.B2.GE41050

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMC IAKGIFKRSL SGKESMFRRA ILLGCSAAKT PWSECSNAQL VDAVKSRKIS FYGLEQALEP DYRRAIEVRR EVVSEIASQQ PEAKKKQSAL HTIPFENYDW NRVVGQNCEN IIGYVPIPLG VAGPILIDGK EYPIPMATTE GALVASTHRG ARAITRSGGC KTLLLGEGMT RAPVVELPSL EEAGRLHKYC NENFLSLKEA FESTTQYGKL NSLKCVLAGR KAYLRFRATT GDAMGMNMIT KGVDKALSVL QQHFPSMEIL ALSGNYCTDK KPSAVNWIDG RGKSVVAEAT LLAEVVEDTL KCTVDSLVSL NIDKNLVGSA MAGSVGGFNA QAANAVAAIF IATGQDPAQV VESSMCITTM SKVGNDLLIS VTMPSIEVGV VGGGTGLAAQ RGCLELIGCG GPSKESPGTN AQLLSR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgca ttgctaaggg aattttcaaa agatccctat ctgggaagga atcgatgttt cgtagggcaa ttcttcttgg atgcagcgct gcgaaaactc catggagtga atgtagtaat gctcaacttg tggatgctgt gaagtcccgc aaaatttcat tttatggctt ggaacaggca ttggagcctg attacaggcg tgccattgag gtgcgtcgtg aggtggtctc cgaaattgct tcccagcagc cagaagccaa gaaaaaacag agtgccctgc atactatccc ctttgagaat tatgactgga acagggttgt cggtcaaaac tgtgaaaata tcataggata tgtaccaatt ccgcttggtg ttgccgggcc aatacttatt gacggaaagg agtaccctat tcctatggct acaacggagg gggcgcttgt agctagcacc catcgtggcg ctcgagccat aacacgatct ggcggttgca agacactttt gttaggtgag ggcatgacaa gggctcccgt ggtggagctt ccatctctgg aggaagcggg aaggcttcat aagtactgta atgaaaattt cttatcacta aaggaggctt ttgagtccac aacgcagtat gggaagctga attcgctaaa gtgtgttttg gcaggacgaa aagcgtatct ccggttccgt gcgaccacgg gtgatgcaat gggaatgaac atgatcacaa aaggcgtgga taaagccttg tcagtcctgc agcagcattt tccttcaatg gagatccttg ctttatcagg taattattgc acagacaaga agccatcagc ggtaaactgg attgatggac gtggaaagtc ggtagtagca gaggctacat tgcttgctga agtcgttgaa gacaccctga aatgcacagt tgacagcctt gtatctctta atattgataa gaatcttgtt ggttcagcta tggctgggtc tgttggtgga ttcaacgcac aagctgcgaa tgccgttgct gccattttta ttgcgacagg gcaggaccca gcgcaggtgg tggagtcttc aatgtgcatt acgaccatgt caaaggtggg caatgatctt cttatctccg tgacaatgcc aagcattgag gttggtgttg ttggaggggg cacaggactg gcggcgcagc gaggctgttt agagttgatt gggtgtggcg gcccgagcaa ggaatcaccg ggtacaaatg cacagttgct ttctcgttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at