TrcrB.18809.a

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34)

CENTER ID: TrcrB.18809.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18809.a.B2.GE41049 mature protein; soluble domain 19 401
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tc00.1047053506831.40
UniProt: Q4D4C9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFRRAILLGC SAAKTPWSEC SNAQLVDAVK SRKISFYGLE QALEPDYRRA IEVRREVVSE IASQQPEAKK KQSALHTIPF ENYDWNKVVG QNCENIIGYV PIPLGVAGPI LIDGKEYPIP MATTEGALVA STHRGARAIT RSGGCKTLLL GEGMTRAPVV ELPSLEEAGR LHKYCNENFL SLKEAFESTT QYGKLNSLKC VLAGRKAYLR FRATTGDAMG MNMITKGVDK ALSVLQQHFP SMEILALSGN YCTDKKPSAV NWIDGRGKSV VAEATLLADV VEDTLKCTVD SLVSLNIDKN LVGSALAGSV GGFNAQAANA VAAIFIATGQ DPAQVVESSM CITTMSKVGN DLLISVTMPS IEVGVVGGGT GLAAQRGCLE LIGCGGPSKE SPGTNAQLLS RVVAAGVLSA ELSLMSGLAA GHLLSAHMRL NRKKK
NT Sequence
atgtttcgta gggcaattct tcttgggtgc agcgctgcga aaactccatg gagtgaatgt agtaatgctc aacttgtgga tgctgtgaag tcccgcaaaa tttcatttta tggcttggaa caggcattgg agcctgatta caggcgtgcc attgaggtgc gtcgtgaggt ggtctccgaa attgcttcgc agcagccaga agccaagaaa aaacagagtg ctctgcatac tatccccttt gagaattatg actggaacaa ggttgtcggt caaaactgtg aaaatatcat aggatatgta ccaattccgc ttggtgttgc cgggccaata cttattgacg gaaaggagta ccctattcct atggctacaa cggagggggc gcttgtagct agcacccatc gtggcgctcg agccataaca cgatctggtg gttgcaagac gcttttgtta ggtgagggca tgacaagggc tcccgtggtg gagcttccat ctctggagga ggcgggaagg cttcataagt actgtaatga aaatttctta tcactaaagg aggcttttga gtccacaacg cagtatggga agctgaattc gctaaagtgt gttttggcag gacgaaaggc gtatctccgg ttccgtgcga ccacgggtga tgcaatggga atgaacatga tcacaaaagg tgtggataaa gccttgtcag tcctgcagca gcattttcct tcaatggaga tccttgcttt atcaggtaat tattgcacag acaagaaacc atcagcggta aactggattg atggacgtgg aaagtcggtc gtagcagagg ctacattgct tgctgacgtc gttgaagaca ccctgaaatg cacagttgac agccttgtat cgcttaacat tgataagaat cttgttggtt cagctctggc tgggtctgtt ggtggattca acgcacaggc tgcgaatgcc gttgcggcca tttttattgc gacagggcag gacccagcgc aggtggtgga gtcttcaatg tgcattacga ccatgtcaaa ggtgggcaat gatcttctta tctccgtcac aatgccaagc attgaggttg gtgttgttgg aggggggaca ggactggcgg cgcagagagg ctgtttagag ttgattgggt gtggcggccc gagcaaggaa tcaccgggta caaatgcaca gttgctttct cgtgtcgtcg ctgcgggtgt actttcagct gagctttctc tcatgtctgg cttggccgcg ggtcatctcc taagcgccca catgcgcctg aatcgcaaaa agaagtga
Details for TrcrB.18809.a.B2.GE41049
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHME CSNAQLVDAV KSRKISFYGL EQALEPDYRR AIEVRREVVS EIASQQPEAK KKQSALHTIP FENYDWNRVV GQNCENIIGY VPIPLGVAGP ILIDGKEYPI PMATTEGALV ASTHRGARAI TRSGGCKTLL LGEGMTRAPV VELPSLEEAG RLHKYCNENF LSLKEAFEST TQYGKLNSLK CVLAGRKAYL RFRATTGDAM GMNMITKGVD KALSVLQQHF PSMEILALSG NYCTDKKPSA VNWIDGRGKS VVAEATLLAE VVEDTLKCTV DSLVSLNIDK NLVGSAMAGS VGGFNAQAAN AVAAIFIATG QDPAQVVESS MCITTMSKVG NDLLISVTMP SIEVGVVGGG TGLAAQRGCL ELIGCGGPSK ESPGTNAQLL SR
Validated NT Sequence
atggctcacc accaccacca ccatatggaa tgtagtaatg ctcaacttgt ggatgctgtg aagtcccgca aaatttcatt ttatggcttg gaacaggcat tggagcctga ttacaggcgt gccattgagg tgcgtcgtga ggtggtctcc gaaattgctt cccagcagcc agaagccaag aaaaaacaga gtgccctgca tactatcccc tttgagaatt atgactggaa cagggttgtc ggtcaaaact gtgaaaatat cataggatat gtaccaattc cgcttggtgt tgccgggcca atacttattg acggaaagga gtaccctatt cctatggcta caacggaggg ggcgcttgta gctagcaccc atcgtggcgc tcgagccata acacgatctg gcggttgcaa gacacttttg ttaggtgagg gcatgacaag ggctcccgtg gtggagcttc catctctgga ggaagcggga aggcttcata agtactgtaa tgaaaatttc ttatcactaa aggaggcttt tgagtccaca acgcagtatg ggaagctgaa ttcgctaaag tgtgttttgg caggacgaaa agcgtatctc cggttccgtg cgaccacggg tgatgcaatg ggaatgaaca tgatcacaaa aggcgtggat aaagccttgt cagtcctgca gcagcatttt ccttcaatgg agatccttgc tttatcaggt aattattgca cagacaagaa gccatcagcg gtaaactgga ttgatggacg tggaaagtcg gtagtagcag aggctacatt gcttgctgaa gtcgttgaag acaccctgaa atgcacagtt gacagccttg tatctcttaa tattgataag aatcttgttg gttcagctat ggctgggtct gttggtggat tcaacgcaca agctgcgaat gccgttgctg ccatttttat tgcgacaggg caggacccag cgcaggtggt ggagtcttca atgtgcatta cgaccatgtc aaaggtgggc aatgatcttc ttatctccgt gacaatgcca agcattgagg ttggtgttgt tggagggggc acaggactgg cggcgcagcg aggctgttta gagttgattg ggtgtggcgg cccgagcaag gaatcaccgg gtacaaatgc acagttgctt tctcgttaa
Expected Protein Sequence
MAHHHHHHEC SNAQLVDAVK SRKISFYGLE QALEPDYRRA IEVRREVVSE IASQQPEAKK KQSALHTIPF ENYDWNKVVG QNCENIIGYV PIPLGVAGPI LIDGKEYPIP MATTEGALVA STHRGARAIT RSGGCKTLLL GEGMTRAPVV ELPSLEEAGR LHKYCNENFL SLKEAFESTT QYGKLNSLKC VLAGRKAYLR FRATTGDAMG MNMITKGVDK ALSVLQQHFP SMEILALSGN YCTDKKPSAV NWIDGRGKSV VAEATLLADV VEDTLKCTVD SLVSLNIDKN LVGSALAGSV GGFNAQAANA VAAIFIATGQ DPAQVVESSM CITTMSKVGN DLLISVTMPS IEVGVVGGGT GLAAQRGCLE LIGCGGPSKE SPGTNAQLLS R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaatgta gtaatgctca acttgtggat gctgtgaagt cccgcaaaat ttcattttat ggcttggaac aggcattgga gcctgattac aggcgtgcca ttgaggtgcg tcgtgaggtg gtctccgaaa ttgcttcgca gcagccagaa gccaagaaaa aacagagtgc tctgcatact atcccctttg agaattatga ctggaacaag gttgtcggtc aaaactgtga aaatatcata ggatatgtac caattccgct tggtgttgcc gggccaatac ttattgacgg aaaggagtac cctattccta tggctacaac ggagggggcg cttgtagcta gcacccatcg tggcgctcga gccataacac gatctggtgg ttgcaagacg cttttgttag gtgagggcat gacaagggct cccgtggtgg agcttccatc tctggaggag gcgggaaggc ttcataagta ctgtaatgaa aatttcttat cactaaagga ggcttttgag tccacaacgc agtatgggaa gctgaattcg ctaaagtgtg ttttggcagg acgaaaggcg tatctccggt tccgtgcgac cacgggtgat gcaatgggaa tgaacatgat cacaaaaggt gtggataaag ccttgtcagt cctgcagcag cattttcctt caatggagat ccttgcttta tcaggtaatt attgcacaga caagaaacca tcagcggtaa actggattga tggacgtgga aagtcggtcg tagcagaggc tacattgctt gctgacgtcg ttgaagacac cctgaaatgc acagttgaca gccttgtatc gcttaacatt gataagaatc ttgttggttc agctctggct gggtctgttg gtggattcaa cgcacaggct gcgaatgccg ttgcggccat ttttattgcg acagggcagg acccagcgca ggtggtggag tcttcaatgt gcattacgac catgtcaaag gtgggcaatg atcttcttat ctccgtcaca atgccaagca ttgaggttgg tgttgttgga ggggggacag gactggcggc gcagagaggc tgtttagagt tgattgggtg tggcggcccg agcaaggaat caccgggtac aaatgcacag ttgctttctc gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat