SSGCID: Target Page

TrcrB.18771.b

leucyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18771.b
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18771.b.B1.GE42705	full length		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.509679.9
UniProt:	Q4CL52

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTARRDKLL CLEAAAQERW AQEKLHEQNA PLKGEPVPSK FFVTFPYPYM NGRLHLGHAF SLTKCEFASR FWRMKGLRSL WPFGLHVTGT PIAACAQKIQ KEMQLYGNPP QFPEEMLGTP SEEKKDDIAT IGQHKSKRGK AAPAKPQWII MRSMGIPDNE IAKFADPLYW LDYFPPLAVE DLKRFGCHID FRRSFITTDR NPYYDRFVSW QFRHLRRANV LGFGKRYCVY SPWDGQPCAD HDRAVGEGAL PQEYTLVKLK VQNATEHPA

NT Sequence

atgtcgacag cacgtcgtga taaattgttg tgccttgagg ctgccgcgca ggagcggtgg gcgcaagaga agttacatga gcagaacgcc ccgctgaagg gcgagccagt tcccagcaag ttttttgtca ccttcccgta cccgtacatg aacgggcgtc tgcatctcgg ccatgcgttt tctctcacaa agtgtgaatt cgcatcacgc ttttggcgaa tgaaggggct tcgctcgctc tggccatttg gactgcatgt caccggaacc cccattgccg cctgtgcgca gaaaattcag aaggaaatgc agctgtacgg aaacccgcca caattcccag aggagatgtt ggggactccg tcagaggaga agaaagacga cattgccacc attggacagc acaaaagtaa gcgtggcaaa gctgctcccg ccaagccaca gtggattatt atgcgaagta tgggtatccc tgataatgag attgcaaaat ttgcggatcc actttattgg cttgattact ttcctcccct tgccgtggag gacctaaaac ggtttggctg ccacattgac tttcgtcgtt cttttataac cacggatcgc aacccatact atgaccgctt tgtttcatgg cagttccggc acttgaggcg ggcgaatgta cttgggtttg gtaaacgata ctgcgtttac tccccatggg acggccaacc ctgcgcggat catgaccggg cggttggcga aggggccttg ccacaggagt atacactggt aaagttgaag gtgcagaacg caacagagca tcctgct

Details for TrcrB.18771.b.B1.GE42705

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS TARRDKLLSL EAAAQERWAQ EKLHEQNAPL KGEPVPSKFF VTFPYPYMNG RLHLGHAFSL TKSEFASRFW RMKGFRSLWP FGLHVTGTPI AACAQKIQKE MQLYGNPPQF PEEMLGTPSE EKKDDIATIG QHKSKRGKAA PAKPQWIIMR SMGIPDNEIA KFSDPLYWLD YFPPLAVEDL KRFGCHIDFR RSFITTDRNP YYDRFVSWQF RHLRRANVLG FGKRYCVYSP WDGQPCADHD RAVGEGALPQ EYTLVKLKVQ NATEHPA

Validated NT Sequence

atcttactca cttaagcagg atgctctgtt gcgttctgca ccttcaactt taccagtgta tactcctgtg gcaaggcccc ttcgccaact gcccggtcat gatccgcaca gggctggccg tcccatgggg agtaaacgca gtatcgttta ccaaacccaa gtacattcgc ccgcctcaag tgccggaact gccatgaaac aaagcggtca tagtatgggt tgcgatccgt ggttataaaa gaacgacgaa agtcaatgtg gcagccaaac cgttttaggt cctccacggc aaggggagga aagtaatcaa gccaataaag tggatccgaa aattttgcaa tctcattatc agggataccc atacttcgca taataatcca ctgtggcttg gcgggagcag ctttgccacg cttacttttg tgctgtccaa tggtggcaat gtcgtctttc ttctcctctg acggagtccc caacatctcc tctgggaatt gtggcgggtt tccgtacagc tgcatttcct tctgaatttt ctgcgcacag gcggcaatgg gggttccggt gacatgcagt ccaaatggcc agagcgagcg aaaccccttc attcgccaaa agcgggatgc gaattcactc tttgtgagag aaaacgcatg gccgagatgc agacgcccgt tcatgtacgg gtacgggaag gtgacaaaaa acttgctggg aactggctcg cccttcagcg gggcgttctg ctcatgtaac ttctcttgcg cccaccgctc ctgcgcggca gcctcaaggc tcaacaattt atcacgacgt gctgtcgaca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHMS TARRDKLLCL EAAAQERWAQ EKLHEQNAPL KGEPVPSKFF VTFPYPYMNG RLHLGHAFSL TKCEFASRFW RMKGLRSLWP FGLHVTGTPI AACAQKIQKE MQLYGNPPQF PEEMLGTPSE EKKDDIATIG QHKSKRGKAA PAKPQWIIMR SMGIPDNEIA KFADPLYWLD YFPPLAVEDL KRFGCHIDFR RSFITTDRNP YYDRFVSWQF RHLRRANVLG FGKRYCVYSP WDGQPCADHD RAVGEGALPQ EYTLVKLKVQ NATEHPA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga cagcacgtcg tgataaattg ttgtgccttg aggctgccgc gcaggagcgg tgggcgcaag agaagttaca tgagcagaac gccccgctga agggcgagcc agttcccagc aagttttttg tcaccttccc gtacccgtac atgaacgggc gtctgcatct cggccatgcg ttttctctca caaagtgtga attcgcatca cgcttttggc gaatgaaggg gcttcgctcg ctctggccat ttggactgca tgtcaccgga acccccattg ccgcctgtgc gcagaaaatt cagaaggaaa tgcagctgta cggaaacccg ccacaattcc cagaggagat gttggggact ccgtcagagg agaagaaaga cgacattgcc accattggac agcacaaaag taagcgtggc aaagctgctc ccgccaagcc acagtggatt attatgcgaa gtatgggtat ccctgataat gagattgcaa aatttgcgga tccactttat tggcttgatt actttcctcc ccttgccgtg gaggacctaa aacggtttgg ctgccacatt gactttcgtc gttcttttat aaccacggat cgcaacccat actatgaccg ctttgtttca tggcagttcc ggcacttgag gcgggcgaat gtacttgggt ttggtaaacg atactgcgtt tactccccat gggacggcca accctgcgcg gatcatgacc gggcggttgg cgaaggggcc ttgccacagg agtatacact ggtaaagttg aaggtgcaga acgcaacaga gcatcctgct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat