SSGCID: Target Page

TrcrB.18771.a

leucyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18771.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18771.a.B1.GE42646	full length		1	1076

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18771.a.B1.PW38540	full length		1	1076

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.511049.40
UniProt:	Q4CTR0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTARRDKLL SLEAAAQERW AQEKLHEQNA PLKGEPVPSK FFVTFPYPYM NGRLHLGHAF SLTKSEFASR FWRMKGFRSL WPFGLHVTGT PIAACAQKIQ KEMQLYGNPP QFPEEMLGTP SEEKKDDIAT IGQHKSKRGK AAPAKPQWII MRSMGIPDNE IAKFSDPLYW LDYFPPLAVE DLKRFGCHID FRRSFITTDR NPYYDRFVSW QFRHLRRANV LGFGKRYCVY SPWDGQPCAD HDRAVGEGAL PQEYTLVKLK VQNATEHPAF LAFKHIIGDR PVILPGATLR VETVCGQTNC WISPNFSYKA YTILNKTGEE EIFIMTSRAA RNMAYQDYVI NGKTGVDPSP LFEVEGAKLV GLPLSAPLCP YATIYTLPMQ TITEAKGTGV VMSVPSDSPD DYINFTQLVR KPDYRSKLGI KDEWVDFDMV PIIEVPGEMG RESAKFICEK LKINGPQATD LLEEAKKVVY QAGFYQGVMI AGPFTGEKTS VAKVKTLQLL TKEDSAIRYY EPSRLVMSRS GDECVVALCD QWYIEYGKKE WKEAVLKHLE NMNMFFPGVR NGFEETLNWL ADWPCSRTLG LGTKLPCDES GTMLIDSLSD STIYMAYYTI AHFIHTSPEG KLRLDGRHDN VLGVTPEMFT DETFDYVFLG KGTPESVHAV NGLPMDAAEK MRREFTFWYP VDLRCSAKDL IQNHLTMFLY THAAIWPDDT SKWPRAIFAN GHIQVDNEKM SKSKGNFMSL AETIDLYGSD ATRLACADAG DSLDDANFVR ETAAGFILKL TTAIDGAQEL LGKKHTLRSG EYNIFDKIFS NSMNFIILQT ERFYVGMQFR GVLNLGFFEL SNEFSQYKLN CDELQIHADL AKRYVEVMTL LLAPLAPHFA EYIWTNVLGN KKSVVCAAFP EPTGPVEYAI LVANRVMTDV VKEIRSQVAK MQKKRGPVDE VFIYTAASYS EWQLKALRLL RELYEANGKS FPVDFSKKVM SHKQEWMTKE ILPDVMAFLS FTKMNVERCG EEALAETPAI NDAELLKGVL ANICKLSGVP TVHILGHEDE RYPEHQAARR KCRPGEPAIV LPAKKK

NT Sequence

atgtcgacag cacgtcgtga taaattgttg agccttgagg ctgccgcgca ggagcggtgg gcgcaagaga agttacatga gcagaacgcc ccgctgaagg gcgagccagt tcccagcaag ttttttgtca ccttcccgta cccgtacatg aacgggcgtc tgcatctcgg ccatgcgttt tctctcacaa agagtgaatt cgcatcccgc ttttggcgaa tgaaggggtt tcgctcgctc tggccatttg gactgcatgt caccggaacc cccattgccg cctgtgcgca gaaaattcag aaggaaatgc agctgtacgg aaacccgcca caattcccag aggagatgtt ggggactccg tcagaggaga agaaagacga cattgccacc attggacagc acaaaagtaa gcgtggcaaa gctgctcccg ccaagccaca gtggattatt atgcgaagta tgggtatccc tgataatgag attgcaaaat tttcggatcc actttattgg cttgattact ttcctcccct tgccgtggag gacctaaaac ggtttggctg ccacattgac tttcgtcgtt cttttataac cacggatcgc aacccatact atgaccgctt tgtttcatgg cagttccggc acttgaggcg ggcgaatgta cttgggtttg gtaaacgata ctgcgtttac tccccatggg acggccagcc ctgtgcggat catgaccggg cagttggcga aggggccttg ccacaggagt atacactggt aaagttgaag gtgcagaacg caacagagca tcctgcgttt ttggcattta aacatattat aggtgaccgc cctgttatcc ttcctggagc aacgttgcgt gtagagactg tgtgcggcca gacgaattgt tggatctccc ctaattttag ttacaaggcg tacacaattt tgaacaagac cggtgaggag gaaattttca tcatgacatc acgtgctgca cggaatatgg cataccagga ctacgtgatc aacggcaaga cgggagtgga tccttctcca ttgtttgaag tggagggggc gaagcttgtt gggctgcctc tctctgcccc cctctgcccc tacgccacta tctacacctt accaatgcag accatcacag aggcaaaggg caccggtgtg gtgatgtccg tcccgtccga tagcccggat gattatatca attttacgca gcttgtaagg aagcccgatt accgttccaa gctgggcatt aaagatgagt gggtggattt tgacatggtt cccatcattg aggtgcccgg tgaaatgggc cgtgagagcg caaagttcat atgtgaaaaa ctgaagatta atggaccaca ggccacagat cttctagagg aagccaagaa agttgtctat caagcgggtt tttaccaggg cgttatgatt gctggcccat tcacgggcga aaaaacctct gttgcaaagg taaagacgtt acaactgctg accaaggaag actctgcaat tcgctactat gaaccttcgc gtcttgtcat gagtcgatct ggtgatgagt gtgtcgtggc attgtgtgat caatggtaca ttgaatatgg gaaaaaagag tggaaagagg cagtgttgaa gcaccttgag aatatgaata tgttctttcc tggagtgcgc aatggatttg aggaaacact taattggctt gcggactggc catgtagccg tactcttgga ctgggtacca agttaccgtg tgatgagagt gggaccatgt tgattgatag tttgagcgat tcaaccatct acatggcata ctacaccatt gctcacttca ttcacacctc acctgagggg aagctacgcc ttgatggacg tcacgacaat gtgttgggtg tcacaccaga gatgtttaca gatgaaacgt ttgactatgt gtttcttggt aaaggtaccc cagagagtgt tcatgcggtc aatggacttc cgatggatgc ggcagaaaag atgcgtcggg agttcacatt ttggtatccc gttgatctcc gctgctccgc gaaggatctt attcaaaatc acttgaccat gttcctctac acacatgcag ctatttggcc agacgacaca agcaagtggc cacgagccat ctttgccaac ggccacatcc aggtggataa cgagaagatg tccaagtcca agggcaactt tatgtccctc gcagaaacga tcgatctgta cggcagtgat gccacacgtc tggcctgtgc agatgcaggg gacagtctcg atgatgcaaa ttttgtccgt gaaactgccg ctgggtttat tttaaagttg accacggcaa tagatggagc gcaagaactg ctgggcaaga aacataccct acgcagcggg gagtacaaca tatttgacaa aatattcagc aactccatga acttcattat tttgcaaact gaaaggttct atgtgggcat gcagtttcgt ggcgtgctca accttggatt ttttgaactt tcgaatgaat tcagtcaata taagttgaat tgcgatgaac ttcagatcca tgcagatctt gctaagcgat atgtggaggt catgacgctt cttttggcac cgttggcccc acactttgca gaatacattt ggacaaatgt attgggcaac aagaaatcgg ttgtctgtgc cgcattccct gaaccaacag gaccggtgga atatgccata cttgttgcaa atcgtgttat gacagatgtg gtgaaggaga tacgctctca ggtggcgaaa atgcagaaga aacgtggtcc cgtggacgag gtgttcatct acactgcggc aagctactca gaatggcaac ttaaagccct acgactgttg cgtgaattat acgaagcgaa tggaaagtcc ttcccggtgg acttttcaaa gaaggtgatg tcgcataaac aagagtggat gacaaaagaa attcttcctg atgtcatggc gttcctctcc ttcaccaaga tgaatgtgga gcgatgtggt gaggaggcgc tcgcggagac gcctgccatc aacgatgcag agctgctgaa gggcgtcctt gccaatatct gcaagttgtc cggtgtgccg accgtgcata ttctcggcca cgaggacgag aggtacccgg agcaccaggc ggcaaggcgt aagtgtcgcc cgggtgagcc cgccatagtg ctcccagcga agaagaagtg a

Details for TrcrB.18771.a.B1.GE42646

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	123kDa
OBSERVED MW:	123kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS TARRDKLLSL EAAAQERWAQ EKLHEQNAPL KGEPVPSKFF VTFPYPYMNG RLHLGHAFSL TKSEFASRFW RMKGFRSLWP FGLHVTGTPI AACAQKIQKE MQLYGNPPQF PEEMLGTPSE EKKDDIATIG QHKSKRGKAA PAKPQWIIMR SMGIPDNEIA KFSDPLYWLD YFPPLAVEDL KRFGCHIDFR RSFITTDRNP YYDRFVSWQF RHLRRANVLG FGKRYCVYSP WDGQPCADHD RAVGEGALPQ EYTLVKLKVQ NATEHPAFLA FKHIIGDRPV ILPGATLRVE TVCGQTNCWI SPNFSYKAYT ILNKTGEEEI FIMTSRAARN MAYQDYVING KTGVDPSPLF EVEGAKLVGL PLSAPLCPYA TIYTLPMQTI TEAKGTGVVM SVPSDSPDDY INFTQLVRKP DYRSKLGIKD EWVDFDMVPI IEVPGEMGRE SAKFICEKLK INGPQATDLL EEAKKVVYQA GFYQGVMIAG PFTGEKTSVA KVKTLQLLTK EDSAIRYYEP SRLVMSRSGD ECVVALCDQW YIEYGKKEWK EAVLKHLENM NMFFPGVRNG FEETLNWLAD WPCSRTLGLG TKLPCDESGT MLIDSLSDST IYMAYYTIAH FIHTSPEGKL RLDGRHDNVL GVTPEMFTDE TFDYVFLGKG TPESVHAVNG LPMDAAEKMR REFTFWYPVD LRCSAKDLIQ NHLTMFLYTH AAIWPDDTSK WPRAIFANGH IQVDNEKMSK SKGNFMSLAE TIDLYGSDAT RLACADAGDS LDDANFVRET AAGFILKLTT AIDGAQELLG KKHTLRSGEY NIFDKIFSNS MNFIILQTER FYVGMQFRGV LNLGFFELSN EFSQYKLNCD ELQIHADLAK RYVEVMTLLL APLAPHFAEY IWTNVLGNKK SVVCAAFPEP TGPVEYAILV ANRVMTDVVK EIRSQVAKMQ KKRGPVDEVF IYTAASYSEW QLKALRLLRE LYEANGKSFP VDFSKKVMSH KQEWMTKEIL PDVMAFLSFT KMNVERCGEE ALAETPAIND AELLKGVLAN ICKLSGVPTV HILGHEDERY PEHQAARRKC RPGEPAIVLP AKKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga cagcacgtcg tgataaattg ttgagccttg aggctgccgc gcaggagcgg tgggcgcaag agaagttaca tgagcagaac gccccgctga agggcgagcc agttcccagc aagttttttg tcaccttccc gtacccgtac atgaacgggc gtctgcatct cggccatgcg ttttctctca caaagagtga attcgcatcc cgcttttggc gaatgaaggg gtttcgctcg ctctggccat ttggactgca tgtcaccgga acccccattg ccgcctgtgc gcagaaaatt cagaaggaaa tgcagctgta cggaaacccg ccacaattcc cagaggagat gttggggact ccgtcagagg agaagaaaga cgacattgcc accattggac agcacaaaag taagcgtggc aaagctgctc ccgccaagcc acagtggatt attatgcgaa gtatgggtat ccctgataat gagattgcaa aattttcgga tccactttat tggcttgatt actttcctcc ccttgccgtg gaggacctaa aacggtttgg ctgccacatt gactttcgtc gttcttttat aaccacggat cgcaacccat actatgaccg ctttgtttca tggcagttcc ggcacttgag gcgggcgaat gtacttgggt ttggtaaacg atactgcgtt tactccccat gggacggcca gccctgtgcg gatcatgacc gggcagttgg cgaaggggcc ttgccacagg agtatacact ggtaaagttg aaggtgcaga acgcaacaga gcatcctgcg tttttggcat ttaaacatat tataggtgac cgccctgtta tccttcctgg agcaacgttg cgtgtagaga ctgtgtgcgg ccagacgaat tgttggatct cccctaattt tagttacaag gcgtacacaa ttttgaacaa gaccggtgag gaggaaattt tcatcatgac atcacgtgct gcacggaata tggcatacca ggactacgtg atcaacggca agacgggagt ggatccttct ccattgtttg aagtggaggg ggcgaagctt gttgggctgc ctctctctgc ccccctctgc ccctacgcca ctatctacac cttaccaatg cagaccatca cagaggcaaa gggcaccggt gtggtgatgt ccgtcccgtc cgatagcccg gatgattata tcaattttac gcagcttgta aggaagcccg attaccgttc caagctgggc attaaagatg agtgggtgga ttttgacatg gttcccatca ttgaggtgcc cggtgaaatg ggccgtgaga gcgcaaagtt catatgtgaa aaactgaaga ttaatggacc acaggccaca gatcttctag aggaagccaa gaaagttgtc tatcaagcgg gtttttacca gggcgttatg attgctggcc cattcacggg cgaaaaaacc tctgttgcaa aggtaaagac gttacaactg ctgaccaagg aagactctgc aattcgctac tatgaacctt cgcgtcttgt catgagtcga tctggtgatg agtgtgtcgt ggcattgtgt gatcaatggt acattgaata tgggaaaaaa gagtggaaag aggcagtgtt gaagcacctt gagaatatga atatgttctt tcctggagtg cgcaatggat ttgaggaaac acttaattgg cttgcggact ggccatgtag ccgtactctt ggactgggta ccaagttacc gtgtgatgag agtgggacca tgttgattga tagtttgagc gattcaacca tctacatggc atactacacc attgctcact tcattcacac ctcacctgag gggaagctac gccttgatgg acgtcacgac aatgtgttgg gtgtcacacc agagatgttt acagatgaaa cgtttgacta tgtgtttctt ggtaaaggta ccccagagag tgttcatgcg gtcaatggac ttccgatgga tgcggcagaa aagatgcgtc gggagttcac attttggtat cccgttgatc tccgctgctc cgcgaaggat cttattcaaa atcacttgac catgttcctc tacacacatg cagctatttg gccagacgac acaagcaagt ggccacgagc catctttgcc aacggccaca tccaggtgga taacgagaag atgtccaagt ccaagggcaa ctttatgtcc ctcgcagaaa cgatcgatct gtacggcagt gatgccacac gtctggcctg tgcagatgca ggggacagtc tcgatgatgc aaattttgtc cgtgaaactg ccgctgggtt tattttaaag ttgaccacgg caatagatgg agcgcaagaa ctgctgggca agaaacatac cctacgcagc ggggagtaca acatatttga caaaatattc agcaactcca tgaacttcat tattttgcaa actgaaaggt tctatgtggg catgcagttt cgtggcgtgc tcaaccttgg attttttgaa ctttcgaatg aattcagtca atataagttg aattgcgatg aacttcagat ccatgcagat cttgctaagc gatatgtgga ggtcatgacg cttcttttgg caccgttggc cccacacttt gcagaataca tttggacaaa tgtattgggc aacaagaaat cggttgtctg tgccgcattc cctgaaccaa caggaccggt ggaatatgcc atacttgttg caaatcgtgt tatgacagat gtggtgaagg agatacgctc tcaggtggcg aaaatgcaga agaaacgtgg tcccgtggac gaggtgttca tctacactgc ggcaagctac tcagaatggc aacttaaagc cctacgactg ttgcgtgaat tatacgaagc gaatggaaag tccttcccgg tggacttttc aaagaaggtg atgtcgcata aacaagagtg gatgacaaaa gaaattcttc ctgatgtcat ggcgttcctc tccttcacca agatgaatgt ggagcgatgt ggtgaggagg cgctcgcgga gacgcctgcc atcaacgatg cagagctgct gaagggcgtc cttgccaata tctgcaagtt gtccggtgtg ccgaccgtgc atattctcgg ccacgaggac gagaggtacc cggagcacca ggcggcaagg cgtaagtgtc gcccgggtga gcccgccata gtgctcccag cgaagaagaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for TrcrB.18771.a.B1.PW38540

PURIFICATION DATe:	10/9/2018
CONCENTRATION:	21.06mg/ml
OBSERVED MW:	123kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCGA CAGCACGTCG TGATAAATTG TTGAGCCTTG AGGCTGCCGC GCAGGAGCGG TGGGCGCAAG AGAAGTTACA TGAGCAGAAC GCCCCGCTGA AGGGCGAGCC AGTTCCCAGC AAGTTTTTTG TCACCTTCCC GTACCCGTAC ATGAACGGGC GTCTGCATCT CGGCCATGCG TTTTCTCTCA CAAAGAGTGA ATTCGCATCC CGCTTTTGGC GAATGAAGGG GTTTCGCTCG CTCTGGCCAT TTGGACTGCA TGTCACCGGA ACCCCCATTG CCGCCTGTGC GCAGAAAATT CAGAAGGAAA TGCAGCTGTA CGGAAACCCG CCACAATTCC CAGAGGAGAT GTTGGGGACT CCGTCAGAGG AGAAGAAAGA CGACATTGCC ACCATTGGAC AGCACAAAAG TAAGCGTGGC AAAGCTGCTC CCGCCAAGCC ACAGTGGATT ATTATGCGAA GTATGGGTAT CCCTGATAAT GAGATTGCAA AATTTTCGGA TCCACTTTAT TGGCTTGATT ACTTTCCTCC CCTTGCCGTG GAGGACCTAA AACGGTTTGG CTGCCACATT GACTTTCGTC GTTCTTTTAT AACCACGGAT CGCAACCCAT ACTATGACCG CTTTGTTTCA TGGCAGTTCC GGCACTTGAG GCGGGCGAAT GTACTTGGGT TTGGTAAACG ATACTGCGTT TACTCCCCAT GGGACGGCCA GCCCTGTGCG GATCATGACC GGGCAGTTGG CGAAGGGGCC TTGCCACAGG AGTATACACT GGTAAAGTTG AAGGTGCAGA ACGCAACAGA GCATCCTGCG TTTTTGGCAT TTAAACATAT TATAGGTGAC CGCCCTGTTA TCCTTCCTGG AGCAACGTTG CGTGTAGAGA CTGTGTGCGG CCAGACGAAT TGTTGGATCT CCCCTAATTT TAGTTACAAG GCGTACACAA TTTTGAACAA GACCGGTGAG GAGGAAATTT TCATCATGAC ATCACGTGCT GCACGGAATA TGGCATACCA GGACTACGTG ATCAACGGCA AGACGGGAGT GGATCCTTCT CCATTGTTTG AAGTGGAGGG GGCGAAGCTT GTTGGGCTGC CTCTCTCTGC CCCCCTCTGC CCCTACGCCA CTATCTACAC CTTACCAATG CAGACCATCA CAGAGGCAAA GGGCACCGGT GTGGTGATGT CCGTCCCGTC CGATAGCCCG GATGATTATA TCAATTTTAC GCAGCTTGTA AGGAAGCCCG ATTACCGTTC CAAGCTGGGC ATTAAAGATG AGTGGGTGGA TTTTGACATG GTTCCCATCA TTGAGGTGCC CGGTGAAATG GGCCGTGAGA GCGCAAAGTT CATATGTGAA AAACTGAAGA TTAATGGACC ACAGGCCACA GATCTTCTAG AGGAAGCCAA GAAAGTTGTC TATCAAGCGG GTTTTTACCA GGGCGTTATG ATTGCTGGCC CATTCACGGG CGAAAAAACC TCTGTTGCAA AGGTAAAGAC GTTACAACTG CTGACCAAGG AAGACTCTGC AATTCGCTAC TATGAACCTT CGCGTCTTGT CATGAGTCGA TCTGGTGATG AGTGTGTCGT GGCATTGTGT GATCAATGGT ACATTGAATA TGGGAAAAAA GAGTGGAAAG AGGCAGTGTT GAAGCACCTT GAGAATATGA ATATGTTCTT TCCTGGAGTG CGCAATGGAT TTGAGGAAAC ACTTAATTGG CTTGCGGACT GGCCATGTAG CCGTACTCTT GGACTGGGTA CCAAGTTACC GTGTGATGAG AGTGGGACCA TGTTGATTGA TAGTTTGAGC GATTCAACCA TCTACATGGC ATACTACACC ATTGCTCACT TCATTCACAC CTCACCTGAG GGGAAGCTAC GCCTTGATGG ACGTCACGAC AATGTGTTGG GTGTCACACC AGAGATGTTT ACAGATGAAA CGTTTGACTA TGTGTTTCTT GGTAAAGGTA CCCCAGAGAG TGTTCATGCG GTCAATGGAC TTCCGATGGA TGCGGCAGAA AAGATGCGTC GGGAGTTCAC ATTTTGGTAT CCCGTTGATC TCCGCTGCTC CGCGAAGGAT CTTATTCAAA ATCACTTGAC CATGTTCCTC TACACACATG CAGCTATTTG GCCAGACGAC ACAAGCAAGT GGCCACGAGC CATCTTTGCC AACGGCCACA TCCAGGTGGA TAACGAGAAG ATGTCCAAGT CCAAGGGCAA CTTTATGTCC CTCGCAGAAA CGATCGATCT GTACGGCAGT GATGCCACAC GTCTGGCCTG TGCAGATGCA GGGGACAGTC TCGATGATGC AAATTTTGTC CGTGAAACTG CCGCTGGGTT TATTTTAAAG TTGACCACGG CAATAGATGG AGCGCAAGAA CTGCTGGGCA AGAAACATAC CCTACGCAGC GGGGAGTACA ACATATTTGA CAAAATATTC AGCAACTCCA TGAACTTCAT TATTTTGCAA ACTGAAAGGT TCTATGTGGG CATGCAGTTT CGTGGCGTGC TCAACCTTGG ATTTTTTGAA CTTTCGAATG AATTCAGTCA ATATAAGTTG AATTGCGATG AACTTCAGAT CCATGCAGAT CTTGCTAAGC GATATGTGGA GGTCATGACG CTTCTTTTGG CACCGTTGGC CCCACACTTT GCAGAATACA TTTGGACAAA TGTATTGGGC AACAAGAAAT CGGTTGTCTG TGCCGCATTC CCTGAACCAA CAGGACCGGT GGAATATGCC ATACTTGTTG CAAATCGTGT TATGACAGAT GTGGTGAAGG AGATACGCTC TCAGGTGGCG AAAATGCAGA AGAAACGTGG TCCCGTGGAC GAGGTGTTCA TCTACACTGC GGCAAGCTAC TCAGAATGGC AACTTAAAGC CCTACGACTG TTGCGTGAAT TATACGAAGC GAATGGAAAG TCCTTCCCGG TGGACTTTTC AAAGAAGGTG ATGTCGCATA AACAAGAGTG GATGACAAAA GAAATTCTTC CTGATGTCAT GGCGTTCCTC TCCTTCACCA AGATGAATGT GGAGCGATGT GGTGAGGAGG CGCTCGCGGA GACGCCTGCC ATCAACGATG CAGAGCTGCT GAAGGGCGTC CTTGCCAATA TCTGCAAGTT GTCCGGTGTG CCGACCGTGC ATATTCTCGG CCACGAGGAC GAGAGGTACC CGGAGCACCA GGCGGCAAGG CGTAAGTGTC GCCCGGGTGA GCCCGCCATA GTGCTCCCAG CGAAGAAGAA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT