SSGCID: Target Page

TrcrB.18755.a

glycyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18755.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18755.a.B2.GE42644	soluble domain		1	298

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.504017.79
UniProt:	Q4D0Z5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRTALPSKLE NLVRAEFEDT CRRRFFFGLA FDPYGGSAGL YDLGPPLCAM KANLLSYWRQ HFVLEENMCE VDTTSLTPEE VFKASGHVVR FNDVMVRDTV TGECIRADKL LEEYSETRLK DATLPIETKQ EMQTLRNRAA GMTPAEMKAV IEHHQVNSPK GNALSEPFPF NLMFSTSIGP EGDRVGYMRP ELAQGIILNF KRLLDSGNAQ RMPFAGACIG TAFRNEIAPR ANLIRVREFT LAEIEHFVNP NDKSHEKFES VRGTEFWAWP RELQDANADP VLMTVGDAVA KKIIDNETLG YFIARTVLFL EAVGLRFLRF RNTSVMR

NT Sequence

atgcgcacgg cactcccgtc gaagctcgag aatttggtgc gggcggagtt tgaggacaca tgccgccgcc gctttttttt cggtctggcc tttgacccgt acggcgggtc ggcggggctg tacgacctgg gcccgccgct ctgcgccatg aaggccaacc tcctgtcgta ctggcggcag cactttgtgc tggaggagaa catgtgtgag gtggacacga cgtccctgac gccggaggag gtgttcaagg catccggcca tgtggtgcgg ttcaacgacg tcatggtgcg ggacaccgtc acaggcgagt gcatccgcgc cgacaagctg ctcgaggagt acagtgagac gcggctcaag gacgccaccc tgccaataga aacgaagcag gagatgcaga cgctccgtaa cagggcggcc gggatgacgc cggcggagat gaaggcggtg attgaacacc accaggtcaa ttcgcccaaa ggcaacgcac tcagcgagcc gtttccgttc aatctcatgt tctccacgag tattggaccg gagggagacc gtgttgggta catgcgccct gaacttgcgc aggggatcat actgaacttt aagagactac tcgattcagg caatgcgcag cgcatgccgt ttgcaggggc gtgcatcggc accgccttcc gaaacgaaat tgcgccacgc gcaaacctta tccgcgtccg tgagtttacg ctggcggaga ttgagcactt tgtcaacccc aacgacaaga gccatgaaaa gtttgagagc gtcaggggca cggagttctg ggcatggcca cgggaactac aggacgccaa tgcagaccct gttcttatga ccgttgggga tgccgttgct aaaaagatca ttgacaatga gacgctcggc tacttcattg cccgcacggt tctttttctt gaggccgtcg gtttgcgttt tctccgcttc cgcaacacca gcgtgatgag atg

Details for TrcrB.18755.a.B2.GE42644

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR TALPSKLENL VRAEFEDTCR RRFFFGLAFD PYGGSAGLYD LGPPLCAMKA NLLSYWRQHF VLEENMCEVD TTSLTPEEVF KASGHVVRFN DVMVRDTVTG ECIRADKLLE EYSETRLKDA TLPIETKQEM QTLRNRAAGM TPAEMKAVIE HHQVNSPKGN ALSEPFPFNL MFSTSIGPEG DRVGYMRPEL AQGIILNFKR LLDSGNAQRM PFAGACIGTA FRNEIAPRAN LIRVREFTLA EIEHFVNPND KSHEKFESVR GTEFWAWPRE LQDANADPVL MTVGDAVAKX XIDNET

Validated NT Sequence

ttactcactt acgtctcatt gtcaatnnnn nntttagcaa cggcatcccc aacggtcata agaacagggt ctgcattggc gtcctgtagt tcccgtggcc atgcccagaa ctccgtgccc ctgacgctct caaacttttc atggctcttg tcgttggggt tgacaaagtg ctcaatctcc gccagcgtaa actcacggac gcggataagg tttgcgcgtg gcgcaatttc gtttcggaag gcggtgccga tgcacgcccc tgcaaacggc atgcgctgcg cattgcctga atcgagtagt ctcttaaagt tcagtatgat cccctgcgca agttcagggc gcatgtaccc aacacggtct ccctccggtc caatactcgt ggagaacatg agattgaacg gaaacggctc gctgagtgcg ttgcctttgg gcgaattgac ctggtggtgt tcaatcaccg ccttcatctc cgccggcgtc atcccggccg ccctgttacg gagcgtctgc atctcctgct tcgtttctat tggcagggtg gcgtccttga gccgcgtctc actgtactcc tcgagcagct tgtcggcgcg gatgcactcg cctgtgacgg tgtcccgcac catgacgtcg ttgaaccgca ccacatggcc ggatgccttg aacacctcct ccggcgtcag ggacgtcgtg tccacctcac acatgttctc ctccagcaca aagtgctgcc gccagtacga caggaggttg gccttcatgg cgcagagcgg cgggcccagg tcgtacagcc ccgccgaccc gccgtacggg tcaaaggcca gaccgaaaaa aaagcggcgg cggcatgtgt cctcaaactc cgcccgcacc aaattctcga gcttcgacgg gagtgccgtg cgcatatggt ggtggtggtg gtgagccatg gtatatctc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgca cggcactccc gtcgaagctc gagaatttgg tgcgggcgga gtttgaggac acatgccgcc gccgcttttt tttcggtctg gcctttgacc cgtacggcgg gtcggcgggg ctgtacgacc tgggcccgcc gctctgcgcc atgaaggcca acctcctgtc gtactggcgg cagcactttg tgctggagga gaacatgtgt gaggtggaca cgacgtccct gacgccggag gaggtgttca aggcatccgg ccatgtggtg cggttcaacg acgtcatggt gcgggacacc gtcacaggcg agtgcatccg cgccgacaag ctgctcgagg agtacagtga gacgcggctc aaggacgcca ccctgccaat agaaacgaag caggagatgc agacgctccg taacagggcg gccgggatga cgccggcgga gatgaaggcg gtgattgaac accaccaggt caattcgccc aaaggcaacg cactcagcga gccgtttccg ttcaatctca tgttctccac gagtattgga ccggagggag accgtgttgg gtacatgcgc cctgaacttg cgcaggggat catactgaac tttaagagac tactcgattc aggcaatgcg cagcgcatgc cgtttgcagg ggcgtgcatc ggcaccgcct tccgaaacga aattgcgcca cgcgcaaacc ttatccgcgt ccgtgagttt acgctggcgg agattgagca ctttgtcaac cccaacgaca agagccatga aaagtttgag agcgtcaggg gcacggagtt ctgggcatgg ccacgggaac tacaggacgc caatgcagac cctgttctta tgaccgttgg ggatgccgtt gctaaaaaga tcattgacaa tgagacgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at