TrcrB.18754.b

Phenylalanyl-tRNA synthetase, beta subunit, putative (PheRS)

CENTER ID: TrcrB.18754.b
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18754.b.B1.GE41833 full length 1 625
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18754.b.B1.PW38398 full length 1 625

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.506127.30
UniProt: Q4DP96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTLAVARDY LFHLMGKNYT EEQFEDVCFK FGVELDDVTS EREMFSREQG MVGGANLEEL SDEVIYKIDT PANRYDLQCA EGMAVALNVF LGLMRAPAFK VLNRCSPLYR MTVDKSVRNV RDYVVCAVLK NICLNERSYR SFIDFQEKLH SGLARRRTLA SVGTHDLDKI GDNNFIYTLK SRESIRFVPL NQRGRMLDCA GDGLAEFYKD DRQISKYVPL ISGLSHYPVI MDREKQSILS LPPIINSDFS CISQETKNIF IECTAPDHYK ANVLVNQIVC AFSTYCEEPF TVEAVRVEYE EATPDGTKVE VTPNLDPAVI PVYIPKVERL IGIKLDSPQH CGQLLERMLH RVAKMEGDNI FVEVPATRPD VLGPTDLMED VAIAYGYDNI KYVECTTRGK VTQQPLSKLS HLLRIEMANA GYTELLTFSL CSRDEAFAHL NREDDDVAVH IANPRTMEFQ ICRPSLMPGI LKTLNANKSH PLPLRFFECS DVVLIDNEKN FPPLIRPHSE YPNCGACNQR HVAALHCCSE NSGFEDIHGL VEFIMKKLSI SRKGEQNAEA EEDTYTLERG TDGAFFPGRC MDIFLHRKDQ KVCLGGFGVV HPNTLRAYDI PFPCSYMEIN IQFAQ
NT Sequence
atgccaactc ttgccgttgc acgcgattac ctgtttcacc tcatggggaa aaattacacg gaggagcagt ttgaagatgt gtgctttaag tttggggttg aacttgacga tgtcacaagt gaacgcgaga tgttcagccg tgaacagggg atggtagggg gcgcgaattt ggaagaactc tccgacgagg ttatttacaa gattgacacc cccgccaacc gctacgacct gcagtgtgcg gagggaatgg cagtggcgct gaatgtgttt ctcgggttga tgcgggctcc cgctttcaag gtgctgaacc gatgcagccc attgtacagg atgaccgtgg ataaatccgt gcggaatgtc cgtgactatg ttgtctgcgc cgtgctaaag aacatctgcc ttaacgagcg cagctaccgc agcttcattg acttccagga gaaactgcac tctggactcg cccgtcgtcg caccctggcc tctgtgggaa cacacgacct ggacaagatt ggggacaaca acttcattta caccttaaaa tcaagggaat ccattcgctt tgttccactg aaccaacgcg gtcgcatgct cgattgcgcc ggagacggtc tagcagagtt ctacaaggac gatcgacaaa tctcgaagta tgtcccgctc atttcgggcc ttagccatta tccagttatc atggaccgtg agaaacaaag catcctctca cttccaccca tcattaactc tgatttttca tgcatcagtc aggagaccaa aaacatcttc atagaatgca ccgcacccga tcactacaag gcaaatgtac tggtgaacca gatcgtgtgt gccttctcaa cgtattgtga agagcccttt acggtcgaag cggtgcgggt ggaatatgag gaagcgacac cggacggcac gaaagtggaa gtgacgccca acttggaccc tgctgtcata cccgtatata ttccaaaggt ggaacggcta atcggcatca agttggactc tccgcagcac tgtggtcaac ttctcgagcg tatgctgcat cgcgttgcca agatggaggg tgacaacatc ttcgttgagg ttcctgcgac ccgaccagat gtgcttgggc caacggatct catggaagat gttgccattg cctatggtta tgataacatc aaatatgttg aatgcaccac tcgaggtaaa gtgacacagc agccactcag taagttgtct catttattgc ggattgagat ggcaaatgca ggatacaccg aacttttaac gttttctttg tgctcccgcg atgaggcatt tgctcatctg aaccgtgagg atgacgatgt tgcagtgcac atcgccaacc cacgaacgat ggaattccaa atctgtcgtc catccctgat gccaggtatt ctcaaaacac tcaatgcaaa caagtcgcat ccattgccgt tgcgcttctt tgaatgttca gatgtggtgc tcatagacaa tgagaagaac tttccgcctc ttatccggcc acattccgaa tacccaaatt gtggcgcatg caaccaacgt catgttgcgg ccctgcattg ctgtagtgaa aacagtggct ttgaagacat tcatggcttg gttgaattta tcatgaaaaa acttagcatt tcgcgaaagg gagaacagaa cgccgaggct gaagaagaca catacacatt ggaaagagga actgatggtg cctttttccc tggaaggtgc atggatattt tcttgcaccg caaggatcaa aaagtgtgcc taggaggctt cggtgttgtc cacccgaaca cactacgggc gtatgatatt ccctttccgt gctcatacat ggagataaat atccagtttg cccagtaa
Details for TrcrB.18754.b.B1.GE41833
HARVESTED ON: 8/7/2017
SEQUENCED ON: 8/12/2017
EXPECTED MW: 72kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMP TLAVARDYLF HLMGKNYTEE QFEDVCFKFG VELDDVTSER EMFSREQGMV GGANLEELSD EVIYKIDTPA NRYDLQCAEG MAVALNVFLG LMRAPAFKVL NRCSPLYRMT VDKSVRNVRD YVVCAVLKNI CLNERSYRSF IDFQEKLHSG LARRRTLASV GTHDLDKIGD NNFIYTLKSR ESIRFVPLNQ RGRMLDCAGD GLAEFYKDDR QISKYVPLIS GLSHYPVIMD REKQSILSLP PIINSDFSCI SQETKNIFIE CTAPDHYKAN VLVNQIVCAF STYCEEPFTV EAVRVEYEEA TPDGTKVEVT PNLDPAVIPV YIPKVERLIG IKLDSPQHCG QLLERMLHRV AKMEGDNIFV EVPATRPDVL GPTDLMEDVA IAYGYDNIKY VECTTRGKVT QQPLSKLSHL LRIEMANAGY TELLTFSLCS RDEAFAHLNR EDDDVAVHIA NPRTMEFQIC RPSLMPGILK TLNANKSHPL PLRFFECSDV VLIDNEKNFP PLIRPHSEYP NCGACNQRHV AALHCCSENS GFEDIHGLVE FIMKKLSISR KGEQNAEAEE DTYTLERGTD GAFFPGRCMD IFLHRKDQKV CLGGFGVVHP NTLRAYDIPF PCSYMEINIQ FAQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa ctcttgccgt tgcacgcgat tacctgtttc acctcatggg gaaaaattac acggaggagc agtttgaaga tgtgtgcttt aagtttgggg ttgaacttga cgatgtcaca agtgaacgcg agatgttcag ccgtgaacag gggatggtag ggggcgcgaa tttggaagaa ctctccgacg aggttattta caagattgac acccccgcca accgctacga cctgcagtgt gcggagggaa tggcagtggc gctgaatgtg tttctcgggt tgatgcgggc tcccgctttc aaggtgctga accgatgcag cccattgtac aggatgaccg tggataaatc cgtgcggaat gtccgtgact atgttgtctg cgccgtgcta aagaacatct gccttaacga gcgcagctac cgcagcttca ttgacttcca ggagaaactg cactctggac tcgcccgtcg tcgcaccctg gcctctgtgg gaacacacga cctggacaag attggggaca acaacttcat ttacacctta aaatcaaggg aatccattcg ctttgttcca ctgaaccaac gcggtcgcat gctcgattgc gccggagacg gtctagcaga gttctacaag gacgatcgac aaatctcgaa gtatgtcccg ctcatttcgg gccttagcca ttatccagtt atcatggacc gtgagaaaca aagcatcctc tcacttccac ccatcattaa ctctgatttt tcatgcatca gtcaggagac caaaaacatc ttcatagaat gcaccgcacc cgatcactac aaggcaaatg tactggtgaa ccagatcgtg tgtgccttct caacgtattg tgaagagccc tttacggtcg aagcggtgcg ggtggaatat gaggaagcga caccggacgg cacgaaagtg gaagtgacgc ccaacttgga ccctgctgtc atacccgtat atattccaaa ggtggaacgg ctaatcggca tcaagttgga ctctccgcag cactgtggtc aacttctcga gcgtatgctg catcgcgttg ccaagatgga gggtgacaac atcttcgttg aggttcctgc gacccgacca gatgtgcttg ggccaacgga tctcatggaa gatgttgcca ttgcctatgg ttatgataac atcaaatatg ttgaatgcac cactcgaggt aaagtgacac agcagccact cagtaagttg tctcatttat tgcggattga gatggcaaat gcaggataca ccgaactttt aacgttttct ttgtgctccc gcgatgaggc atttgctcat ctgaaccgtg aggatgacga tgttgcagtg cacatcgcca acccacgaac gatggaattc caaatctgtc gtccatccct gatgccaggt attctcaaaa cactcaatgc aaacaagtcg catccattgc cgttgcgctt ctttgaatgt tcagatgtgg tgctcataga caatgagaag aactttccgc ctcttatccg gccacattcc gaatacccaa attgtggcgc atgcaaccaa cgtcatgttg cggccctgca ttgctgtagt gaaaacagtg gctttgaaga cattcatggc ttggttgaat ttatcatgaa aaaacttagc atttcgcgaa agggagaaca gaacgccgag gctgaagaag acacatacac attggaaaga ggaactgatg gtgccttttt ccctggaagg tgcatggata ttttcttgca ccgcaaggat caaaaagtgt gcctaggagg cttcggtgtt gtccacccga acacactacg ggcgtatgat attccctttc cgtgctcata catggagata aatatccagt ttgcccagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for TrcrB.18754.b.B1.PW38398
PURIFICATION DATe: 12/4/2017
CONCENTRATION: 33.83mg/ml
OBSERVED MW: 75kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
Two pellet purification. Combined LSEID 43294 and 43368
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMP TLAVARDYLF HLMGKNYTEE QFEDVCFKFG VELDDVTSER EMFSREQGMV GGANLEELSD EVIYKIDTPA NRYDLQCAEG MAVALNVFLG LMRAPAFKVL NRCSPLYRMT VDKSVRNVRD YVVCAVLKNI CLNERSYRSF IDFQEKLHSG LARRRTLASV GTHDLDKIGD NNFIYTLKSR ESIRFVPLNQ RGRMLDCAGD GLAEFYKDDR QISKYVPLIS GLSHYPVIMD REKQSILSLP PIINSDFSCI SQETKNIFIE CTAPDHYKAN VLVNQIVCAF STYCEEPFTV EAVRVEYEEA TPDGTKVEVT PNLDPAVIPV YIPKVERLIG IKLDSPQHCG QLLERMLHRV AKMEGDNIFV EVPATRPDVL GPTDLMEDVA IAYGYDNIKY VECTTRGKVT QQPLSKLSHL LRIEMANAGY TELLTFSLCS RDEAFAHLNR EDDDVAVHIA NPRTMEFQIC RPSLMPGILK TLNANKSHPL PLRFFECSDV VLIDNEKNFP PLIRPHSEYP NCGACNQRHV AALHCCSENS GFEDIHGLVE FIMKKLSISR KGEQNAEAEE DTYTLERGTD GAFFPGRCMD IFLHRKDQKV CLGGFGVVHP NTLRAYDIPF PCSYMEINIQ FAQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCAA CTCTTGCCGT TGCACGCGAT TACCTGTTTC ACCTCATGGG GAAAAATTAC ACGGAGGAGC AGTTTGAAGA TGTGTGCTTT AAGTTTGGGG TTGAACTTGA CGATGTCACA AGTGAACGCG AGATGTTCAG CCGTGAACAG GGGATGGTAG GGGGCGCGAA TTTGGAAGAA CTCTCCGACG AGGTTATTTA CAAGATTGAC ACCCCCGCCA ACCGCTACGA CCTGCAGTGT GCGGAGGGAA TGGCAGTGGC GCTGAATGTG TTTCTCGGGT TGATGCGGGC TCCCGCTTTC AAGGTGCTGA ACCGATGCAG CCCATTGTAC AGGATGACCG TGGATAAATC CGTGCGGAAT GTCCGTGACT ATGTTGTCTG CGCCGTGCTA AAGAACATCT GCCTTAACGA GCGCAGCTAC CGCAGCTTCA TTGACTTCCA GGAGAAACTG CACTCTGGAC TCGCCCGTCG TCGCACCCTG GCCTCTGTGG GAACACACGA CCTGGACAAG ATTGGGGACA ACAACTTCAT TTACACCTTA AAATCAAGGG AATCCATTCG CTTTGTTCCA CTGAACCAAC GCGGTCGCAT GCTCGATTGC GCCGGAGACG GTCTAGCAGA GTTCTACAAG GACGATCGAC AAATCTCGAA GTATGTCCCG CTCATTTCGG GCCTTAGCCA TTATCCAGTT ATCATGGACC GTGAGAAACA AAGCATCCTC TCACTTCCAC CCATCATTAA CTCTGATTTT TCATGCATCA GTCAGGAGAC CAAAAACATC TTCATAGAAT GCACCGCACC CGATCACTAC AAGGCAAATG TACTGGTGAA CCAGATCGTG TGTGCCTTCT CAACGTATTG TGAAGAGCCC TTTACGGTCG AAGCGGTGCG GGTGGAATAT GAGGAAGCGA CACCGGACGG CACGAAAGTG GAAGTGACGC CCAACTTGGA CCCTGCTGTC ATACCCGTAT ATATTCCAAA GGTGGAACGG CTAATCGGCA TCAAGTTGGA CTCTCCGCAG CACTGTGGTC AACTTCTCGA GCGTATGCTG CATCGCGTTG CCAAGATGGA GGGTGACAAC ATCTTCGTTG AGGTTCCTGC GACCCGACCA GATGTGCTTG GGCCAACGGA TCTCATGGAA GATGTTGCCA TTGCCTATGG TTATGATAAC ATCAAATATG TTGAATGCAC CACTCGAGGT AAAGTGACAC AGCAGCCACT CAGTAAGTTG TCTCATTTAT TGCGGATTGA GATGGCAAAT GCAGGATACA CCGAACTTTT AACGTTTTCT TTGTGCTCCC GCGATGAGGC ATTTGCTCAT CTGAACCGTG AGGATGACGA TGTTGCAGTG CACATCGCCA ACCCACGAAC GATGGAATTC CAAATCTGTC GTCCATCCCT GATGCCAGGT ATTCTCAAAA CACTCAATGC AAACAAGTCG CATCCATTGC CGTTGCGCTT CTTTGAATGT TCAGATGTGG TGCTCATAGA CAATGAGAAG AACTTTCCGC CTCTTATCCG GCCACATTCC GAATACCCAA ATTGTGGCGC ATGCAACCAA CGTCATGTTG CGGCCCTGCA TTGCTGTAGT GAAAACAGTG GCTTTGAAGA CATTCATGGC TTGGTTGAAT TTATCATGAA AAAACTTAGC ATTTCGCGAA AGGGAGAACA GAACGCCGAG GCTGAAGAAG ACACATACAC ATTGGAAAGA GGAACTGATG GTGCCTTTTT CCCTGGAAGG TGCATGGATA TTTTCTTGCA CCGCAAGGAT CAAAAAGTGT GCCTAGGAGG CTTCGGTGTT GTCCACCCGA ACACACTACG GGCGTATGAT ATTCCCTTTC CGTGCTCATA CATGGAGATA AATATCCAGT TTGCCCAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT