TrcrB.18754.a

Phenylalanyl-tRNA synthetase, putative (PheRS)

CENTER ID: TrcrB.18754.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18754.a.B1.GE41832 full length 1 625
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.508271.10
UniProt: Q4CXE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTLAVARDY LFHLMGKKYT EEQFEDVCFE FGVELDDVTS EREMFSREQG MVGGTNLEEL SDEVIYKIDT PANRYDLQCA EGMAVALKVF LGLMRAPAFK VLNRCSPLYR MTVDKSVRNV RDYVVCAVLK NIRLNERSYR SFIDFQEKLH SGLARRRTLA SVGTHDLDKI GDNNFIYTLK PKESIRFVPL NQRGRMLDCA GDGLAEFYKD DRQISKYVPL ISGLSHYPVI MDREKQSILS LPPIINSDFS CISQETKNIF IECTAPDHYK ANVLVNQIVC AFSTYCEEPF TVEAVRVEYE EATPDGTTVE VTPNLDSAVI PVNIPKVERL IGIKLDSPQH CGQLLERMLH RVAKMEGDNI FVEVPATRPD VLGPTDLMED VAIAYGYDNI KYVECTTRGK VTQQPLSKLS HLLRIEMANA GYTELLTFSL CSRDEAFAHL NREDDDVAVH IANPRTMEFQ ICRPSLMPGI LKTLHANKSH PLPLRFFECA DVVLIDNEKN FPPLIRPHSE YPNCGACNQR HVAALHCCSE NSGFEDIHGL VEFIMKKLGI SRKGDQNAEA EEDTYTLERG TDGAFFPGRC MDIFLHRKDQ KVCLGGFGVV HPNTLRAYDI PFPCSYMEIN IQFAQ
NT Sequence
atgccaactc ttgccgttgc acgcgattac ttgtttcacc tcatggggaa aaagtacacg gaggagcagt ttgaagatgt gtgctttgag tttggggttg aacttgacga tgtcacaagt gaacgcgaga tgttcagccg tgaacagggg atggtagggg gcacgaattt ggaagaactc tccgacgagg ttatttacaa gattgacacc cccgccaacc gctacgacct gcagtgtgcg gagggaatgg cagtggcgct gaaggtgttt ctcgggttga tgcgggctcc cgctttcaag gtgctgaacc gatgcagccc attatacagg atgaccgtgg ataaatccgt gcggaatgtc cgtgactatg ttgtctgcgc cgtgctaaag aacatccgcc ttaacgagcg cagctaccgc agcttcattg atttccagga gaaactgcat tctggactcg cccgtcgtcg caccctggct tctgtgggga cacacgacct ggacaagatt ggggacaaca acttcattta cacgttaaaa ccaaaggaat ccattcgctt tgttccactg aaccaacgcg gtcgcatgct cgattgcgcc ggagatggtc tagcagagtt ctacaaggac gatcgacaaa tctcgaagta tgtcccgctc atttcgggcc ttagccatta tccagttatc atggaccgtg agaaacaaag catcctctcg cttccaccca tcattaactc tgatttttca tgcatcagcc aggagaccaa aaacatcttc atagaatgca ccgcacccga tcactacaag gcaaatgtac tggtgaacca gatcgtgtgt gccttctcaa cgtattgtga agagcccttt acggtcgaag cggtgcgggt ggaatatgag gaagcgacac cggacggcac gacagtggaa gtgacgccca acttggactc tgctgtcata cccgtaaata ttccaaaggt ggaacggctt atcggcatca agttggactc tccgcagcac tgtggtcaac ttctcgagcg tatgctgcat cgcgttgcca agatggaggg tgacaacatc ttcgttgagg ttcctgcgac ccgaccagat gtgcttgggc caacggatct catggaagat gttgccattg cctatggtta tgataacatc aaatatgttg aatgcaccac tcgaggtaaa gtgacacagc agccactcag taagttgtct catttattgc ggattgagat ggcaaatgca ggatacaccg aacttttaac tttttctttg tgctcccgag atgaggcatt tgctcatctg aaccgtgagg atgacgatgt tgcggtgcac atcgccaacc cacgaacgat ggaattccaa atctgtcgtc catccctgat gccaggtatt ctcaaaacac tccatgcaaa caagtcgcat ccgttgccgt tgcgcttctt tgaatgtgca gatgtggtgc tcatagacaa tgagaagaac tttccgcctc ttatccggcc acattccgag tacccaaatt gcggcgcatg caaccaacgt catgttgccg ccctgcattg ctgtagtgaa aacagtggct ttgaagacat tcatggcttg gttgagttta tcatgaaaaa acttggcatt tcgcgaaagg gagaccagaa cgccgaggct gaagaagaca catacacatt ggaaagagga actgatggtg cctttttccc tggaagatgc atggatattt tcttgcaccg caaggatcaa aaagtgtgcc taggaggctt cggtgttgtc cacccgaaca cactacgggc gtatgatatt ccctttccgt gctcatacat ggagataaat atccagtttg cccagtaa
Details for TrcrB.18754.a.B1.GE41832
HARVESTED ON: 8/7/2017
SEQUENCED ON: 9/20/2017
EXPECTED MW: 72kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 85
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMP TFAVARDYLF HLMGKKYTEE QFEDVCFEFG VELDDVTSER EMFSREQGMV GGTNLEELSD EVIYKIDTPA NRYDLQCAEG MAVALKVFLG LMRAPAFKVL NRCSPLYRMT VDKSVRNVRD YVVCAVLKNI RLNERSYRSF IDFQEKLHSG LARRRTLASV GTHDLDKIGD NNFIYTLKPK ESIRFVPLNQ RGRMLDCAGD GLAEFYKDDR QISKYVPLIS GLSHYPVIMD REKQSILSLP PIINSDFXCI SQXXXXXXXX CXXXXXYKAN VLVXXXVXAX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXRXXHR VAKMXXXNXF VEVXATRPDV LGPTDLMEDV AIAYGYDNIK YVECTTRGKV TQQPLSKLSH LLRIEMANAG YTELLTFSLC SRDEAFAHLN REDDDVAVHI ANPRTMEFQI CRPSLMPGIL KTLHANKSHP LPLRFFECAD VVLIDNEKNF PPLIRPHSEY PNCGACNQRH VAALHCCSEN SGFEDIHGLV EFIMKKLGIS RKGDQNAEAE EDTYTLERGT DGAFFPGRCM DIFLHRKDQK VCLGGFGVVH PNTLRAYDIP FPCSYME
Validated NT Sequence
tctccatgta tgagcacgga aagggaatat catacgcccg tagtgtgttc gggtggacaa caccgaagcc tcctaggcac actttttgat ccttgcggtg caagaaaata tccatgcatc ttccagggaa aaaggcacca tcagttcctc tttccaatgt gtatgtgtct tcttcagcct cggcgttctg gtctcccttt cgcgaaatgc caagtttttt catgataaac tcaaccaagc catgaatgtc ttcaaagcca ctgttttcac tacagcaatg cagggcggca acatgacgtt ggttgcatgc gccgcaattt gggtactcgg aatgtggccg gataagaggc ggaaagttct tctcattgtc tatgagcacc acatctgcac attcaaagaa gcgcaacggc aacggatgcg acttgtttgc atggagtgtt ttgagaatac ctggcatcag ggatggacga cagatttgga attccatcgt tcgtgggttg gcgatgtgca ccgcaacatc gtcatcctca cggttcagat gagcaaatgc ctcatctcgg gagcacaaag aaaaagttaa aagttcggtg tatcctgcat ttgccatctc aatccgcaat aaatgagaca acttactgag tggctgctgt gtcactttac ctcgagtggt gcattcaaca tatttgatgt tatcataacc ataggcaatg gcaacatctt ccatgagatc cgttggccca agcacatctg gtcgggtcgc nnnaacctca acgaagntgt tgtnnnnntc catcttggcn acgcgatgcn nnatacgntn nnnnnnnnnn nnnnnnnnnt gcnnnnnnnc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt nnngannnnn nnnngnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngnn nanaaggcan nnacgannnn nnnnaccagt acatttgcct tgtannnnnn ngntgnnnng catnnnnnnn nnatgntnnn nnnnncctgg ctgatgcann naaaatcaga gttaatgatg ggtggaagcg agaggatgct ttgtttctca cggtccatga taactggata atggctaagg cccgaaatga gcgggacata cttcgagatt tgtcgatcgt ccttgtagaa ctctgctaga ccatctccgg cgcaatcgag catgcgaccg cgttggttca gtggaacaaa gcgaatggat tcctttggtt ttaacgtgta aatgaagttg ttgtccccaa tcttgtccag gtcgtgtgtc cccacagaag ccagggtgcg acgacgggcg agtccagaat gcagtttctc ctggaaatca atgaagctgc ggtagctgcg ctcgttaagg cggatgttct ttagcacggc gcagacaaca tagtcacgga cattccgcac ggatttatcc acggtcatcc tgtataatgg gctgcatcgg ttcagcacct tgaaagcggg agcccgcatc aacccgagaa acaccttcag cgccactgcc attccctccg cacactgcag gtcgtagcgg ttggcggggg tgtcaatctt gtaaataacc tcgtcggaga gttcttccaa attcgtgccc cctaccatcc cctgttcacg gctgaacatc tcgcgttcac ttgtgacatc gtcaagttca accccaaact caaagcacac atcttcaaac tgctcctccg tgtacttttt ccccatgagg tgaaacaagt aatcgcgtgc aacggcaaaa gttggcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMP TLAVARDYLF HLMGKKYTEE QFEDVCFEFG VELDDVTSER EMFSREQGMV GGTNLEELSD EVIYKIDTPA NRYDLQCAEG MAVALKVFLG LMRAPAFKVL NRCSPLYRMT VDKSVRNVRD YVVCAVLKNI RLNERSYRSF IDFQEKLHSG LARRRTLASV GTHDLDKIGD NNFIYTLKPK ESIRFVPLNQ RGRMLDCAGD GLAEFYKDDR QISKYVPLIS GLSHYPVIMD REKQSILSLP PIINSDFSCI SQETKNIFIE CTAPDHYKAN VLVNQIVCAF STYCEEPFTV EAVRVEYEEA TPDGTTVEVT PNLDSAVIPV NIPKVERLIG IKLDSPQHCG QLLERMLHRV AKMEGDNIFV EVPATRPDVL GPTDLMEDVA IAYGYDNIKY VECTTRGKVT QQPLSKLSHL LRIEMANAGY TELLTFSLCS RDEAFAHLNR EDDDVAVHIA NPRTMEFQIC RPSLMPGILK TLHANKSHPL PLRFFECADV VLIDNEKNFP PLIRPHSEYP NCGACNQRHV AALHCCSENS GFEDIHGLVE FIMKKLGISR KGDQNAEAEE DTYTLERGTD GAFFPGRCMD IFLHRKDQKV CLGGFGVVHP NTLRAYDIPF PCSYMEINIQ FAQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccaa ctcttgccgt tgcacgcgat tacttgtttc acctcatggg gaaaaagtac acggaggagc agtttgaaga tgtgtgcttt gagtttgggg ttgaacttga cgatgtcaca agtgaacgcg agatgttcag ccgtgaacag gggatggtag ggggcacgaa tttggaagaa ctctccgacg aggttattta caagattgac acccccgcca accgctacga cctgcagtgt gcggagggaa tggcagtggc gctgaaggtg tttctcgggt tgatgcgggc tcccgctttc aaggtgctga accgatgcag cccattatac aggatgaccg tggataaatc cgtgcggaat gtccgtgact atgttgtctg cgccgtgcta aagaacatcc gccttaacga gcgcagctac cgcagcttca ttgatttcca ggagaaactg cattctggac tcgcccgtcg tcgcaccctg gcttctgtgg ggacacacga cctggacaag attggggaca acaacttcat ttacacgtta aaaccaaagg aatccattcg ctttgttcca ctgaaccaac gcggtcgcat gctcgattgc gccggagatg gtctagcaga gttctacaag gacgatcgac aaatctcgaa gtatgtcccg ctcatttcgg gccttagcca ttatccagtt atcatggacc gtgagaaaca aagcatcctc tcgcttccac ccatcattaa ctctgatttt tcatgcatca gccaggagac caaaaacatc ttcatagaat gcaccgcacc cgatcactac aaggcaaatg tactggtgaa ccagatcgtg tgtgccttct caacgtattg tgaagagccc tttacggtcg aagcggtgcg ggtggaatat gaggaagcga caccggacgg cacgacagtg gaagtgacgc ccaacttgga ctctgctgtc atacccgtaa atattccaaa ggtggaacgg cttatcggca tcaagttgga ctctccgcag cactgtggtc aacttctcga gcgtatgctg catcgcgttg ccaagatgga gggtgacaac atcttcgttg aggttcctgc gacccgacca gatgtgcttg ggccaacgga tctcatggaa gatgttgcca ttgcctatgg ttatgataac atcaaatatg ttgaatgcac cactcgaggt aaagtgacac agcagccact cagtaagttg tctcatttat tgcggattga gatggcaaat gcaggataca ccgaactttt aactttttct ttgtgctccc gagatgaggc atttgctcat ctgaaccgtg aggatgacga tgttgcggtg cacatcgcca acccacgaac gatggaattc caaatctgtc gtccatccct gatgccaggt attctcaaaa cactccatgc aaacaagtcg catccgttgc cgttgcgctt ctttgaatgt gcagatgtgg tgctcataga caatgagaag aactttccgc ctcttatccg gccacattcc gagtacccaa attgcggcgc atgcaaccaa cgtcatgttg ccgccctgca ttgctgtagt gaaaacagtg gctttgaaga cattcatggc ttggttgagt ttatcatgaa aaaacttggc atttcgcgaa agggagacca gaacgccgag gctgaagaag acacatacac attggaaaga ggaactgatg gtgccttttt ccctggaaga tgcatggata ttttcttgca ccgcaaggat caaaaagtgt gcctaggagg cttcggtgtt gtccacccga acacactacg ggcgtatgat attccctttc cgtgctcata catggagata aatatccagt ttgcccagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat