SSGCID: Target Page

TrcrB.18710.a

inositol polyphosphate kinase, putative (IPMK)

CENTER ID:	TrcrB.18710.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18710.a.B1.GE43554	full length		1	334

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.510741.110
UniProt:	Q4DRL4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATCESASHA PEDEPIVVGG HKGVIQRGPK APNDSATITK KSTGWEVLMY LGMALRIDET MCELAAMAPK LVAVLSPEEE NSGVWITVEK EENRPFLEAF HEELREASVH THGYSEVMDT ARWSVCLIDV TDGMLLPCVA DIKIGYIRHS PHTPPEKMAR IKKKRLIQPL AIRLCGALHY FYRTISNEQC IERETCEKDI GYLLHTEEDH RRCLRAFFSS AVSMKQEESG NKCGESEVLS ARLKACCRHV EKLLSFFTSS VGCALLERTA FVSTSLLLLY DAVSRVSDEV GKNDAADVRV YLIDYARVSD RRLNFAEENI GFLRGLENFL FLIS

NT Sequence

atggcgactt gtgagtcggc ttcccacgca ccggaagacg agcccattgt cgttggcggc cacaagggag ttattcaacg aggaccgaag gctcccaacg actccgcgac cattacgaaa aagtctaccg ggtgggaagt gctcatgtac cttggtatgg cacttcgaat tgacgagacg atgtgtgaac tggctgcaat ggcgccaaaa cttgtggcag ttttatctcc tgaggaagaa aattcgggcg tttggataac tgtggaaaag gaagaaaata gacctttttt ggaagcattt cacgaagaac tgcgagaggc aagtgttcac acccatggct acagtgaagt tatggacaca gctcgctggt cggtctgttt gatcgatgta acagacggga tgcttctacc atgtgtggca gacattaaaa tcgggtacat ccgtcactct ccacacacgc cgccggagaa gatggctcgc ataaaaaaaa agcgactgat ccagccatta gccattcgtc tgtgtggtgc tcttcactac ttctaccgca ccatatcgaa cgagcaatgc attgaaaggg aaacgtgtga aaaagacata ggatatctct tacatacaga agaagatcac cgacgctgtt tgcgggcctt tttttcctct gcggtcagca tgaaacaaga agagtcgggg aataaatgcg gcgaaagtga ggtcttgtcc gcgcggttga aagcgtgctg tcggcacgtt gagaaactac tctctttttt taccagctca gtgggctgtg cattattgga aaggaccgct tttgtttcaa catctctttt actgctttac gatgccgtca gccgcgtaag tgacgaagtt ggcaaaaacg acgctgctga tgtgcgtgtc tacctgattg actatgctcg agtaagtgat cgacgcctga actttgcgga agaaaatatt ggctttctac gtggcttaga aaattttctt tttcttattt cgtga

Details for TrcrB.18710.a.B1.GE43554

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA TCESASHAPE DEPIVVGGHK GVIQRGPKAP NDSATITKKS TGWEVLMYLG MALRIDETMC ELAAMAPKLV AVLSPEEENS GVWITVEKEE NRPFLEAFHE ELREASVHTH GYSEVMDTAR WSVCLIDVTD GMLLPCVADI KIGYIRHSPH TPPEKMARIK KKRLIQPLAI RLCGALHYFY RTISNEQCIE RETCEKDIGY LLHTEEDHRR CLRAFFSSAV SMKQEESGNK CGESEVLSAR LKACCRHVEK LLSFFTSSVG CALLERTAFV STSLLLLYDA VSRVSDEVGK NDAADVRVYL IDYARVSDRR LNFAEENIGF LRGLE

Validated NT Sequence

ttttctaagc cacgtagaaa gccaatattt tcttccgcaa agttcaggcg tcgatcactt actcgagcat agtcaatcag gtagacacgc acatcagcag cgtcgttttt gccaacttcg tcacttacgc ggctgacggc atcgtaaagc agtaaaagag atgttgaaac aaaagcggtc ctttccaata atgcacagcc cactgagctg gtaaaaaaag agagtagttt ctcaacgtgc cgacagcacg ctttcaaccg cgcggacaag acctcacttt cgccgcattt attccccgac tcttcttgtt tcatgctgac cgcagaggaa aaaaaggccc gcaaacagcg tcggtgatct tcttctgtat gtaagagata tcctatgtct ttttcacacg tttccctttc aatgcattgc tcgttcgata tggtgcggta gaagtagtga agagcaccac acagacgaat ggctaatggc tggatcagtc gctttttttt tatgcgagcc atcttctccg gcggcgtgtg tggagagtga cggatgtacc cgattttaat gtctgccaca catggtagaa gcatcccgtc tgttacatcg atcaaacaga ccgaccagcg agctgtgtcc ataacttcac tgtagccatg ggtgtgaaca cttgcctctc gcagttcttc gtgaaatgct tccaaaaaag gtctattttc ttccttttcc acagttatcc aaacgcccga attttcttcc tcaggagata aaactgccac aagttttggc gccattgcag ccagttcaca catcgtctcg tcaattcgaa gtgccatacc aaggtacatg agcacttccc acccggtaga ctttttcgta atggtcgcgg agtcgttggg agccttcggt cctcgttgaa taactccctt gtggccgcca acgacaatgg gctcgtcttc cggtgcgtgg gaagccgact cacaagtcgc catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga cttgtgagtc ggcttcccac gcaccggaag acgagcccat tgtcgttggc ggccacaagg gagttattca acgaggaccg aaggctccca acgactccgc gaccattacg aaaaagtcta ccgggtggga agtgctcatg taccttggta tggcacttcg aattgacgag acgatgtgtg aactggctgc aatggcgcca aaacttgtgg cagttttatc tcctgaggaa gaaaattcgg gcgtttggat aactgtggaa aaggaagaaa atagaccttt tttggaagca tttcacgaag aactgcgaga ggcaagtgtt cacacccatg gctacagtga agttatggac acagctcgct ggtcggtctg tttgatcgat gtaacagacg ggatgcttct accatgtgtg gcagacatta aaatcgggta catccgtcac tctccacaca cgccgccgga gaagatggct cgcataaaaa aaaagcgact gatccagcca ttagccattc gtctgtgtgg tgctcttcac tacttctacc gcaccatatc gaacgagcaa tgcattgaaa gggaaacgtg tgaaaaagac ataggatatc tcttacatac agaagaagat caccgacgct gtttgcgggc ctttttttcc tctgcggtca gcatgaaaca agaagagtcg gggaataaat gcggcgaaag tgaggtcttg tccgcgcggt tgaaagcgtg ctgtcggcac gttgagaaac tactctcttt ttttaccagc tcagtgggct gtgcattatt ggaaaggacc gcttttgttt caacatctct tttactgctt tacgatgccg tcagccgcgt aagtgacgaa gttggcaaaa acgacgctgc tgatgtgcgt gtctacctga ttgactatgc tcgagtaagt gatcgacgcc tgaactttgc ggaagaaaat attggctttc tacgtggctt agaaaatttt ctttttctta tttcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat