SSGCID: Target Page

TrcrB.18708.a

isoleucyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18708.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18708.a.B2.GE42642	soluble domain #1		1	663
TrcrB.18708.a.B3.GE42643	soluble domain #2		870	1156

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.509797.40
UniProt:	Q4D2F2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRCLTAVWR CAAKDGKGNG KRASLARRTV IHSRVPSIGP SYASLSSLGG TTNHGELSKC FSSSKAAKGT TEGKMSGNKS VSGGGPLENF DEELNFSKME EEVLQHWERV DAFRETLKQS KGKKPYNFYD GPPFATGLPH YGHLLAGTIK DIVCRYAHQT GHHVERRFGW DCHGLPIEFE IDKTLGIKSS HDVKAYGIAK YNDTCKNIVM RYSEEWRRTV NRMGRWIDFD NDYKTMYLSY MESVWWVFKE LWNKNLVYRG FKVMPFSTGC TTPLSNFEAN SNYKDVSDPS VMVTFQTRDD PNTYLIAWTT TPWTLPSNLA LCVHPNLDYV KVLDTKTKRH YIVGELRLGE VFPSKKKGAH KGDEAPPYVI VTRMKGTELV GTKYEPLFPY FKSTYGETAF RVISDTYVTT DSGTCVVHQA PGFGEEDYRI CISNGILKKE EVLCPVDENG VFHSDVTDFC GRYVKEADSD ILKHLESRGL VHSKGSVVHS YPFCWRSELP LIYKAVDCWF VNVESLRDRL LAANDKTNWV PEFVKTRRFS NWLADAKDWN LSRNRYWGTP LPIWHSEDWE EMVCVGSVRE LEELSGVTGI TDIHRQFVDN ITIPSKRPGM PPLRRVEEVF DCWFESGSMP YAQSHYPFEN QKDFETHRFP ADFVAEGLDQ TRGWFYTLLV LGVALFDVSP FKNVVVNGLI LSEDGKKMSK RLKNYPEPNI IIDTYGADAL RMYMINSPVV RAEPLRFREQ GVRGIVKDVL LPLFHATKFF IANANYCVLS GGTVSLDVVS TNEMDRWILA STQTLQQYVR REMERYHLYN VIPGVFRFVV DLSNWYVRMN RRRMKNAVDQ EDRSQALSTM LTVLFSVSRI LSPIVPFVAE MLYQRIKPLL PTSEQVDSVH YLMFPEDDKG KHDELLERAM TRMITIVDLA RVLRDRMVIS MKRPVRQVII VHPDQSYLDD VRKVAEYIKD EINTFDVVLS SGEEYVSTHL DANMEALGKL YRKDAPQIRK AIQQLTPEEV ARFIQSGKME VCGKLITVDD VKVVRKIKDG FTDFESNTDN DVVVLLDKRE EQALVDSWRA REFVNRVQQL RKKVKLVVTD MVDVYFESGD VELTNSILNC AEQVNKTIRG KWETMDKLPA DAKFVAEEDN SISGVGIKIV FTEVSA

NT Sequence

atgttgcgct gtctcacagc ggtatggcga tgtgcggcaa aagatggtaa agggaacggg aagcgcgctt ctttggcgcg gcgcactgtc attcactctc gtgtgccttc tatcggccct tcctacgctt ccctttcttc gctcggcgga actaccaacc acggcgaact ttcaaagtgt ttttctagct caaaggcagc aaagggaaca actgaaggaa aaatgtctgg taataagagc gtcagtggcg gaggtccact tgaaaatttt gacgaagaac taaacttttc caaaatggag gaagaggtgc tgcaacactg ggagcgggtt gatgcattcc gcgaaaccct caagcagtcg aaaggaaaaa aaccatacaa cttttatgat ggcccaccct ttgccaccgg tttgccccac tacggccacc tcttggctgg caccatcaaa gacattgtct gccgctacgc ccaccaaact ggccaccatg tggaacgccg gtttggatgg gattgccacg ggcttcctat tgagtttgag attgacaaga cacttggaat taaatcttcc cacgatgtga aggcctatgg gattgccaag tacaatgata cgtgtaagaa tattgtgatg cgttattcgg aggagtggcg caggacagta aatcgcatgg ggcgttggat tgactttgat aacgactaca agacgatgta tctttcttac atggagtctg tttggtgggt ttttaaggag ttgtggaata agaatcttgt ttatcgcggg tttaaggtga tgccattttc gacaggatgc acaactccac tcagtaactt tgaggccaat tcaaattata aggatgtgag cgatccatct gtaatggtta ctttccagac aagggatgat ccaaacacgt accttatagc ttggacaacg acaccgtgga cactgccaag taatttggca ctttgtgtac atcccaattt ggactatgtg aaggtcttgg acacaaaaac aaaacgacac tacattgtcg gtgaattgcg ccttggagaa gttttcccaa gcaagaagaa gggtgctcat aaaggagatg aggcaccgcc atacgttatt gttactcgca tgaaaggaac tgaacttgtc gggacaaaat acgaaccact cttcccctat tttaaatcga cctatgggga aaccgctttt cgtgttatta gtgatactta tgttacaaca gatagtggta catgcgtcgt gcatcaagca cctggatttg gtgaggagga ctatcggata tgcatctcta acggtatttt aaagaaagaa gaagtgttgt gtcctgttga tgaaaacggg gtgtttcatt cggatgtgac tgatttttgt ggccgttacg tgaaggaagc ggattcagac attttaaagc acttggagtc gagagggttg gtgcatagca aaggatccgt ggtccatagt tatcctttct gctggcgaag tgaactacct ttgatctaca aggcggtgga ctgttggttt gtcaatgtgg agtcgttacg tgaccgtttg ttggcggcga atgataaaac gaattgggtt cccgagtttg tgaagacccg gcgtttttcc aactggctag cagatgccaa ggattggaat ctttctcgca accgctactg gggcacaccc ctgcccattt ggcacagcga agattgggag gaaatggtgt gcgtcggtag cgtgagggag ttggaggagc tttccggtgt gacgggcatt acagacatac accgacaatt tgtggacaac attacgattc caagtaagcg accaggcatg cctccgctgc gtcgtgtgga ggaggtgttt gactgttggt ttgagtctgg atcgatgccg tatgcccagt ctcactaccc ctttgagaat cagaaggatt ttgagacgca taggtttcca gcggattttg tggcggaagg attggatcag acccggggct ggttttacac cctgcttgtc cttggcgttg ccctttttga cgtctcgccc tttaaaaatg ttgttgtcaa cggtctcatc ctctcggagg atggaaagaa gatgagtaag cggttaaaga actatccgga accaaatatt ataattgata catatggtgc cgatgcactg cgtatgtata tgattaattc tcccgtggtt cgtgccgagc cgctgcgttt ccgcgaacag ggtgtgcgag gcatcgtcaa ggatgtgtta ctaccacttt tccatgcaac taaatttttt attgcaaacg ccaactactg tgtgttgagc ggtggaactg tttctctgga tgtcgtcagc acaaatgaga tggatcgatg gattttggcg tcaacgcaaa ccttgcaaca atatgtacgg cgggagatgg agcggtacca cttgtacaac gtcattccag gtgttttccg gtttgttgtg gatctttcca actggtatgt tcgtatgaat cgccgccgta tgaagaacgc tgtggatcaa gaagatcgtt cacaagcttt atctacgatg ctgacagttc tcttttccgt ttcccgcata ctgtccccca ttgttccgtt tgtggcagag atgttgtacc aacgcatcaa accacttctt cctacgtctg agcaggtgga ttccgtgcac tacctcatgt tccctgaaga tgataagggg aaacatgacg aactattgga acgtgccatg acacgtatga tcaccattgt cgatcttgct cgtgtgcttc gggaccgtat ggtgatctcc atgaagcgtc ctgtaaggca agtcattatt gtgcatcctg atcagtcgta tttggacgat gttcgtaagg tggctgagta tattaaggat gagattaaca catttgatgt ggttctgtca tctggcgaag agtatgtttc aacacacttg gatgccaata tggaggcttt ggggaaacta taccgcaagg atgctcctca gatccgtaag gcgattcagc aactgactcc agaggaggtg gcaaggttca ttcaaagtgg gaagatggag gtgtgcggaa agctcatcac cgtcgacgat gtcaaggtgg tgcgcaagat caaggatggc tttacggatt ttgagagcaa caccgacaac gatgtggtgg tacttttgga caaacgcgag gaacaggctc tggtggattc ctggcgggcg cgggagtttg tcaaccgcgt gcagcagctg cgaaaaaagg tgaagcttgt ggtaacagac atggtggacg tgtactttga gtcaggggac gtggagctca cgaactccat cctcaattgt gcagagcagg tcaacaagac cattcggggc aaatgggaga cgatggataa actaccggca gacgcgaaat ttgttgccga agaggacaac agcatttccg gtgttggcat taagattgtc ttcacggagg tttcggcctg a

Details for TrcrB.18708.a.B2.GE42642

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	77kDa
OBSERVED MW:	77kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML RCLTAVWRCA AKDGKGNGKR ASLARRTVIH SRVPSIGPSY ASLSSLGGTT NHGELSKCFS SSKAAKGTTE GKMSGNKSVS GGGPLENFDE ELNFSKMEEE VLQHWERVDA FRETLKQSKG KKPYNFYDGP PFATGLPHYG HLLAGTIKDI VCRYAHQTGH HVERRFGWDC HGLPIEFEID KTLGIKSSHD VKAYGIAKYN DTCKNIVMRY SEEWRRTVNR MGRWIDFDND YKTMYLSYME SVWWVFKELW NKNLVYRGFK VMPFSTGCTT PLSNFEANSN YKDVSDPSVM VTFQTRDDPN TYLIAWTTTP WTLPSNLALC VHPNLDYVKV LDTKTKRHYI VGELRLGEVF PSKKKGAHKG DEAPPYVIVT RMKGTELVGT KYEPLFPYFK STYGETAFRV ISDTYVTTDS GTCVVHQAPG FGEEDYRICI SNGILKKEEV LCPVDENGVF HSDVTDFCGR YVKEADSDIL KHLESRGLVH SKGSVVHSYP FCWRSELPLI YKAVDCWFVN VESLRDRLLA ANDKTNWVPE FVKTRRFSNW LADAKDWNLS RNRYWGTPLP IWHSEDWEEM VCVGSVRELE ELSGVTGITD IHRQFVDNIT IPSKRPGMPP LRRVEEVFDC WFESGSMPYA QSHYPFENQK DFETHRFPAD FVAEGLDQTR G

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc gctgtctcac agcggtatgg cgatgtgcgg caaaagatgg taaagggaac gggaagcgcg cttctttggc gcggcgcact gtcattcact ctcgtgtgcc ttctatcggc ccttcctacg cttccctttc ttcgctcggc ggaactacca accacggcga actttcaaag tgtttttcta gctcaaaggc agcaaaggga acaactgaag gaaaaatgtc tggtaataag agcgtcagtg gcggaggtcc acttgaaaat tttgacgaag aactaaactt ttccaaaatg gaggaagagg tgctgcaaca ctgggagcgg gttgatgcat tccgcgaaac cctcaagcag tcgaaaggaa aaaaaccata caacttttat gatggcccac cctttgccac cggtttgccc cactacggcc acctcttggc tggcaccatc aaagacattg tctgccgcta cgcccaccaa actggccacc atgtggaacg ccggtttgga tgggattgcc acgggcttcc tattgagttt gagattgaca agacacttgg aattaaatct tcccacgatg tgaaggccta tgggattgcc aagtacaatg atacgtgtaa gaatattgtg atgcgttatt cggaggagtg gcgcaggaca gtaaatcgca tggggcgttg gattgacttt gataacgact acaagacgat gtatctttct tacatggagt ctgtttggtg ggtttttaag gagttgtgga ataagaatct tgtttatcgc gggtttaagg tgatgccatt ttcgacagga tgcacaactc cactcagtaa ctttgaggcc aattcaaatt ataaggatgt gagcgatcca tctgtaatgg ttactttcca gacaagggat gatccaaaca cgtaccttat agcttggaca acgacaccgt ggacactgcc aagtaatttg gcactttgtg tacatcccaa tttggactat gtgaaggtct tggacacaaa aacaaaacga cactacattg tcggtgaatt gcgccttgga gaagttttcc caagcaagaa gaagggtgct cataaaggag atgaggcacc gccatacgtt attgttactc gcatgaaagg aactgaactt gtcgggacaa aatacgaacc actcttcccc tattttaaat cgacctatgg ggaaaccgct tttcgtgtta ttagtgatac ttatgttaca acagatagtg gtacatgcgt cgtgcatcaa gcacctggat ttggtgagga ggactatcgg atatgcatct ctaacggtat tttaaagaaa gaagaagtgt tgtgtcctgt tgatgaaaac ggggtgtttc attcggatgt gactgatttt tgtggccgtt acgtgaagga agcggattca gacattttaa agcacttgga gtcgagaggg ttggtgcata gcaaaggatc cgtggtccat agttatcctt tctgctggcg aagtgaacta cctttgatct acaaggcggt ggactgttgg tttgtcaatg tggagtcgtt acgtgaccgt ttgttggcgg cgaatgataa aacgaattgg gttcccgagt ttgtgaagac ccggcgtttt tccaactggc tagcagatgc caaggattgg aatctttctc gcaaccgcta ctggggcaca cccctgccca tttggcacag cgaagattgg gaggaaatgg tgtgcgtcgg tagcgtgagg gagttggagg agctttccgg tgtgacgggc attacagaca tacaccgaca atttgtggac aacattacga ttccaagtaa gcgaccaggc atgcctccgc tgcgtcgtgt ggaggaggtg tttgactgtt ggtttgagtc tggatcgatg ccgtatgccc agtctcacta cccctttgag aatcagaagg attttgagac gcataggttt ccagcggatt ttgtggcgga aggattggat cagacccggg gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for TrcrB.18708.a.B3.GE42643

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME MLYQRIKPLL PTSEQVDSVH YLMFPEDDKG KHDELLERAM TRMITIVDLA RVLRDRMVIS MKRPVRQVII VHPDQSYLDD VRKVAEYIKD EINTFDVVLS SGEEYVSTHL DANMEALGKL YRKDAPQIRK AIQQLTPEEV ARFIQSGKME VCGKLITVDD VKVVRKIKDG FTDFESNTDN DVVVLLDKRE EQALVDSWRA REFVNRVQQL RKKVKLVVTD MVDVYFESGD VELTNSILNC AEQVNKTIRG KWETMDKLPA DAKFVAEEDN SISGVGIXXX XTEVSA

Validated NT Sequence

atcttactca cttaggccga aacctccgtn nnnnnnnnnt taatgccaac accggaaatg ctgttgtcct cttcggcaac aaatttcgcg tctgccggta gtttatccat cgtctcccat ttgccccgaa tggtcttgtt gacctgctct gcacaattga ggatggagtt cgtgagctcc acgtcccctg actcaaagta cacgtccacc atgtctgtta ccacaagctt cacctttttt cgcagctgct gcacgcggtt gacaaactcc cgcgcccgcc aggaatccac cagagcctgt tcctcgcgtt tgtccaaaag taccaccaca tcgttgtcgg tgttgctctc aaaatccgta aagccatcct tgatcttgcg caccaccttg acatcgtcga cggtgatgag ctttccgcac acctccatct tcccactttg aatgaacctt gccacctcct ctggagtcag ttgctgaatc gccttacgga tctgaggagc atccttgcgg tatagtttcc ccaaagcctc catattggca tccaagtgtg ttgaaacata ctcttcgcca gatgacagaa ccacatcaaa tgtgttaatc tcatccttaa tatactcagc caccttacga acatcgtcca aatacgactg atcaggatgc acaataatga cttgccttac aggacgcttc atggagatca ccatacggtc ccgaagcaca cgagcaagat cgacaatggt gatcatacgt gtcatggcac gttccaatag ttcgtcatgt ttccccttat catcttcagg gaacatgagg tagtgcacgg aatccacctg ctcagacgta ggaagaagtg gtttgatgcg ttggtacaac atctccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHEM LYQRIKPLLP TSEQVDSVHY LMFPEDDKGK HDELLERAMT RMITIVDLAR VLRDRMVISM KRPVRQVIIV HPDQSYLDDV RKVAEYIKDE INTFDVVLSS GEEYVSTHLD ANMEALGKLY RKDAPQIRKA IQQLTPEEVA RFIQSGKMEV CGKLITVDDV KVVRKIKDGF TDFESNTDND VVVLLDKREE QALVDSWRAR EFVNRVQQLR KKVKLVVTDM VDVYFESGDV ELTNSILNCA EQVNKTIRGK WETMDKLPAD AKFVAEEDNS ISGVGIKIVF TEVSA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagatgt tgtaccaacg catcaaacca cttcttccta cgtctgagca ggtggattcc gtgcactacc tcatgttccc tgaagatgat aaggggaaac atgacgaact attggaacgt gccatgacac gtatgatcac cattgtcgat cttgctcgtg tgcttcggga ccgtatggtg atctccatga agcgtcctgt aaggcaagtc attattgtgc atcctgatca gtcgtatttg gacgatgttc gtaaggtggc tgagtatatt aaggatgaga ttaacacatt tgatgtggtt ctgtcatctg gcgaagagta tgtttcaaca cacttggatg ccaatatgga ggctttgggg aaactatacc gcaaggatgc tcctcagatc cgtaaggcga ttcagcaact gactccagag gaggtggcaa ggttcattca aagtgggaag atggaggtgt gcggaaagct catcaccgtc gacgatgtca aggtggtgcg caagatcaag gatggcttta cggattttga gagcaacacc gacaacgatg tggtggtact tttggacaaa cgcgaggaac aggctctggt ggattcctgg cgggcgcggg agtttgtcaa ccgcgtgcag cagctgcgaa aaaaggtgaa gcttgtggta acagacatgg tggacgtgta ctttgagtca ggggacgtgg agctcacgaa ctccatcctc aattgtgcag agcaggtcaa caagaccatt cggggcaaat gggagacgat ggataaacta ccggcagacg cgaaatttgt tgccgaagag gacaacagca tttccggtgt tggcattaag attgtcttca cggaggtttc ggcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat