SSGCID: Target Page

TrcrB.18681.b

prolyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18681.b
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18681.b.B1.GE42641	full length		1	810

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.509805.130
UniProt:	Q4DZZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKMSYGVHSP PALRCWCTLP FSSRRKASSL ENTYFSNRTV FCSSARWIYR RHCEASVSGA DLSSGFFFYS SLNKEQVTNS TRTMSASDCR GESEMLKILR ALGMDLPTIS HEEKHTVEEA NKELGRFGVP CTGTKNIFLK SKKEDLVLVT ALHTTRTNMK VIQDALSLKD LRFAPEDVLR THLGVVQGGV TPLALVNNES KNIKLVLDKA LVESTVPVVL HPCRNDKSCL ITPKQLQDFL RKLEYPFVVV DFSAEATGGT PSGDNTRAKS STSQKETGKK TSQPAAAAIT GGANVTGETK LGILVKREEN FSQWYVEVIT KAEMIEYYDV SGCYIIRPWA FFIWSRIQRF FGARIESMGV EDCYFPMFVS KSCLEREKDH VEGFAPEVAW VTKAGETDLE VPVAIRPTSE TVMYPYYAKW IRSHRDLPVR LNMWNNVIRW EFSHPTPFIR TREFLWQEGH CAWQTEEECS QEVLEILDHY AAVYEELLAV PVVKGRKTEK EKFAGGYYTT TVETYIAAVG RGCQGGTSHN LGQNFGKMFN ISFQDPEKND GSTLIPWQNS WGLSTRVIGV MTMVHGDDSG MVLPPRVASV QVVIIPVGIT KNTTDEERKS LLEGCKTLER VLVSGGIRAK ADLRDNYSPG WRFNHWEVKG VPVRVELGPR ELASLKISLV LRHDGSRRSL LWDKELPTAM TLLMDEIHNS MFAKAKRERQ ENTKKLTEWS DFTPALNRKC LVLAPWCGEM SCEDQVKKDS AEESKAMQSE EVKDDARAPS MGAKTLCIPF DQPESVEGKT CICKSCSRPA KHWVLFGRSY

NT Sequence

atgaaaatga gttacggcgt ccactctcca ccggcgctgc ggtgttggtg cacgttgcct ttcagttcaa ggaggaaggc atcgtctttg gaaaatactt atttcagtaa ccgtactgtc ttttgttctt cagcacgatg gatttaccgc cgtcactgtg aggcgagtgt gtctggtgca gatctttcct ccggcttctt cttttattct tctctcaaca aagagcaggt aacgaattct accagaacga tgtcagcgag tgattgcagg ggcgagtcgg agatgctgaa gattctccga gctcttggta tggatctgcc tacaatttcc cacgaggaga aacatactgt ggaggaagcc aacaaggagc ttgggcgctt tggcgtaccg tgcacgggca ccaaaaacat ttttttgaag agtaagaagg aggacctggt tcttgtgacg gcattacaca ccaccaggac gaacatgaag gtgatacagg atgctttgtc tcttaaagat cttcgctttg ccccggagga cgttttgcgg actcatctgg gcgttgtgca ggggggtgtt accccgctgg cactcgtaaa caacgaatca aagaacatta aattggtgct agacaaggcg ctggtggaaa gtaccgtccc tgtagtcttg cacccgtgcc gtaatgacaa gagctgtctg ataacgccca aacagctgca ggattttctt aggaagctgg agtatccatt tgtggtggtt gacttttcgg cggaagccac gggaggtacc ccgtctggtg ataacacaag ggcaaagagt tccacgtctc aaaaggagac ggggaaaaaa acttctcagc ccgccgcagc tgcaattacc ggtggtgcta atgttactgg tgaaacaaaa ttgggtattc tggtgaagcg tgaggaaaac ttctcacagt ggtacgtcga ggtgattacg aaggctgaga tgatcgagta ctacgatgtg tcaggttgct acatcattcg cccatgggca ttttttatct ggagccgcat ccaaagattt tttggggccc gcattgaaag catgggagtt gaggattgtt actttcccat gtttgtctcc aagtcttgcc tggaacgcga gaaggatcac gtggaaggat ttgcgccgga ggtggcatgg gtgacgaagg caggtgagac ggatcttgag gtgcctgttg ctattcgtcc aacaagtgaa acagtgatgt acccatatta tgcaaagtgg atccgctcac accgtgactt gccagtgagg ctgaacatgt ggaataacgt tattcgctgg gagttttcac atcccacacc gtttattcgt acccgagagt ttctttggca ggagggacac tgcgcgtggc agacggagga ggagtgttcc caggaggtac ttgaaatcct tgatcattac gctgctgtct atgaagaact gctggccgtt ccggtggtga agggaaggaa gacagagaag gagaagtttg ccggcggcta ttacacaaca actgtggaga cgtatatcgc cgcggttggt cgcggttgcc agggtgggac gagccacaat ctgggccaga attttggaaa gatgtttaac atcagcttcc aggatcccga gaagaatgat ggttccactc ttattccgtg gcagaactcg tggggacttt ctactcgcgt tattggcgtc atgacaatgg tccacgggga tgactcgggg atggtgctgc ctccacgcgt cgcttcggtg caggttgtca ttattcctgt gggtattaca aagaacacta cggatgagga gcgaaagtcc ttgctggagg gatgcaagac ccttgaaagg gtgttggtaa gcggtggcat ccgtgccaag gcagaccttc gtgataacta cagccctgga tggaggttca accactggga ggtgaagggc gtgccggtgc gtgtcgaatt ggggccgagg gagctcgcat cattaaaaat atcacttgtc ttgcgccacg atggcagccg tcgctctctt ttatgggaca aggagttgcc cactgcaatg acattgttga tggacgaaat tcataatagc atgtttgcaa aggccaaaag ggagcgtcag gaaaatacaa agaaattgac agagtggagt gactttaccc ctgcactcaa caggaagtgc ttggtgttgg caccgtggtg cggtgagatg tcgtgcgagg accaggtgaa aaaggacagt gcggaggagt caaaggcaat gcagagcgag gaggtgaagg atgatgcacg tgccccaagc atgggtgcta aaacgctctg catcccgttt gatcagccgg agtctgtcga gggcaaaaca tgtatctgca agagctgcag ccgccccgcg aagcactggg tgctctttgg acgcagttac taa

Details for TrcrB.18681.b.B1.GE42641

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	92kDa
OBSERVED MW:	92kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK MSYGVHSPPA LRCWCTLPFS SRRKASSLEN TYFSNRTVFC SSARWIYRRH CEASVSGADL SSGFFFYSSL NKEQVTNSTR TMSASDCRGE SEMLKILRAL GMDLPTISHE EKHTVEEANK ELGRFGVPCT GTKNIFLKSK KEDLVLVTAL HTTRTNMKVI QDALSLKDLR FAPEDVLRTH LGVVQGGVTP LALVNNESKN IKLVLDKALV ESTVPVVLHP CRNDKSCLIT PKQLQDFLRK LEYPFVVVDF SAEATGGTPS GDNTRAKSST SQKETGKKTS QPAAAAITGG ANVTGETKLG ILVKREENFS QWYVEVITKA EMIEYYDVSG CYIIRPWAFF IWSRIQRFFG ARIESMGVED CYFPMFVSKS CLEREKDHVE GFAPEVAWVT KAGETDLEVP VAIRPTSETV MYPYYAKWIR SHRDLPVRLN MWNNVIRWEF SHPTPFIRTR EFLWQEGHCA WQTEEECSQE VLEILDHYAA VYEELLAVPV VKGRKTEKEK FAGGYYTTTV ETYIAAVGRG CQGGTSHNLG QNFGKMFNIS FQDPEKNDGS TLIPWQNSWG LSTRVIGVMT MVHGDDSGMV LPPRVASVQV VIIPVGITKN TTDEERKSLL EGCKTLERVL VSGGIRAKAD LRDNYSPGWR FNHWEVKGVP VRVELGPREL ASLKISLVLR HDGSRRSLLW DKELPTAMTL LMDEIHNSMF AKAKRERQEN TKKLTEWSDF TPALNRKCLV LAPWCGEMSC EDQVKKDSAE ESKAMQSEEV KDDARAPSMG AKTLCIPFDQ PESVEGKTCI CKSCSRPAKH WVLFGRSY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tgagttacgg cgtccactct ccaccggcgc tgcggtgttg gtgcacgttg cctttcagtt caaggaggaa ggcatcgtct ttggaaaata cttatttcag taaccgtact gtcttttgtt cttcagcacg atggatttac cgccgtcact gtgaggcgag tgtgtctggt gcagatcttt cctccggctt cttcttttat tcttctctca acaaagagca ggtaacgaat tctaccagaa cgatgtcagc gagtgattgc aggggcgagt cggagatgct gaagattctc cgagctcttg gtatggatct gcctacaatt tcccacgagg agaaacatac tgtggaggaa gccaacaagg agcttgggcg ctttggcgta ccgtgcacgg gcaccaaaaa catttttttg aagagtaaga aggaggacct ggttcttgtg acggcattac acaccaccag gacgaacatg aaggtgatac aggatgcttt gtctcttaaa gatcttcgct ttgccccgga ggacgttttg cggactcatc tgggcgttgt gcaggggggt gttaccccgc tggcactcgt aaacaacgaa tcaaagaaca ttaaattggt gctagacaag gcgctggtgg aaagtaccgt ccctgtagtc ttgcacccgt gccgtaatga caagagctgt ctgataacgc ccaaacagct gcaggatttt cttaggaagc tggagtatcc atttgtggtg gttgactttt cggcggaagc cacgggaggt accccgtctg gtgataacac aagggcaaag agttccacgt ctcaaaagga gacggggaaa aaaacttctc agcccgccgc agctgcaatt accggtggtg ctaatgttac tggtgaaaca aaattgggta ttctggtgaa gcgtgaggaa aacttctcac agtggtacgt cgaggtgatt acgaaggctg agatgatcga gtactacgat gtgtcaggtt gctacatcat tcgcccatgg gcatttttta tctggagccg catccaaaga ttttttgggg cccgcattga aagcatggga gttgaggatt gttactttcc catgtttgtc tccaagtctt gcctggaacg cgagaaggat cacgtggaag gatttgcgcc ggaggtggca tgggtgacga aggcaggtga gacggatctt gaggtgcctg ttgctattcg tccaacaagt gaaacagtga tgtacccata ttatgcaaag tggatccgct cacaccgtga cttgccagtg aggctgaaca tgtggaataa cgttattcgc tgggagtttt cacatcccac accgtttatt cgtacccgag agtttctttg gcaggaggga cactgcgcgt ggcagacgga ggaggagtgt tcccaggagg tacttgaaat ccttgatcat tacgctgctg tctatgaaga actgctggcc gttccggtgg tgaagggaag gaagacagag aaggagaagt ttgccggcgg ctattacaca acaactgtgg agacgtatat cgccgcggtt ggtcgcggtt gccagggtgg gacgagccac aatctgggcc agaattttgg aaagatgttt aacatcagct tccaggatcc cgagaagaat gatggttcca ctcttattcc gtggcagaac tcgtggggac tttctactcg cgttattggc gtcatgacaa tggtccacgg ggatgactcg gggatggtgc tgcctccacg cgtcgcttcg gtgcaggttg tcattattcc tgtgggtatt acaaagaaca ctacggatga ggagcgaaag tccttgctgg agggatgcaa gacccttgaa agggtgttgg taagcggtgg catccgtgcc aaggcagacc ttcgtgataa ctacagccct ggatggaggt tcaaccactg ggaggtgaag ggcgtgccgg tgcgtgtcga attggggccg agggagctcg catcattaaa aatatcactt gtcttgcgcc acgatggcag ccgtcgctct cttttatggg acaaggagtt gcccactgca atgacattgt tgatggacga aattcataat agcatgtttg caaaggccaa aagggagcgt caggaaaata caaagaaatt gacagagtgg agtgacttta cccctgcact caacaggaag tgcttggtgt tggcaccgtg gtgcggtgag atgtcgtgcg aggaccaggt gaaaaaggac agtgcggagg agtcaaaggc aatgcagagc gaggaggtga aggatgatgc acgtgcccca agcatgggtg ctaaaacgct ctgcatcccg tttgatcagc cggagtctgt cgagggcaaa acatgtatct gcaagagctg cagccgcccc gcgaagcact gggtgctctt tggacgcagt tactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat