TrcrB.18664.b

kinetoplastid kinetochore protein 3, putative (kkt3)

CENTER ID: TrcrB.18664.b
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18664.b.B2.GE43005 PHoranyi kinase domain truncation 1 320
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.508461.230
UniProt: Q4E4T9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLGSVETISY DGEFVRLVEL RLPLRGSTQG QLLSIRTCER IGQGSFGTVY RAVCDGYPKL ALKIATGKAA RLRQELDVLV KVCTKGKLLL PRFEFGAINK TGDLIVVGME LCVPSTLHDL LLVTRLTNEA DMLFLAYQVL QAVACVHEQQ CIHRDVKLQN FVFDLDGNLK LIDFGLASSA WNPPPGDVVA GTVSFMAPEM AHNALHRDQR VSVGAPADVW SAGIVLFSIF TQRNPYPPSI ASITSPHSDE DEDGDGDGAA VARKLNRENL RLLRRVATAK WRWPVGSSVS NELRQLVEFI LVTDPVKRPD ISTILRKPIW NLRRRAPPTA VTAFLGVQDE LLLSHDETHL LRAVEQRSAD VTASLMGSRA NSLDGDGDEE AERRSSLKFV AREQTAVGGV PTHQVYDIRR ESRQKRPLRE ISVVIAEETA KGLRRRQPKR ARGDVSSVSR GNSCAGSRIN SHANSRATSP ARQCSVHECV QSLVKGKKNG KMSRTIPQAS SVIFDDEEEE GVQREVEQMV TKKEKPFVAT QRIQKKQRNG GVCSHQCLRS SRIGDEAENT KGLLRGGING SAVDISSGAV LELPKCQMGL TKAVDGETRT KKVPIHVHCD RKEQVSVWID DMQLVAMEKS QRELAEKRIF LELECLFSAL RLTTEENQAW FDMVWLAEEQ RKSAAHPHRF KETTRVSKKY QYGFVCDMCD YEFLPAGKTI HFFHCTCGRD LCVDCHRVYS KQCTCAECGA IYENSVALRG HHRKGGCKAV VKNRRSSVAY EGQVSANGPL MRRKTAAQTH DMPRGATVAT GSKATTKAAA AAKSFPRSEA VRMGAVNTVK KEAVAKEKTA SSLFSRPINT PPEGQWKPME RLSVSHKPIH PTPEEREVLL NGDWIRHFHL FPTEEEAEPV AFTYHIQPGR TGAIFLNHKF SMHSAVISVL ERQMLIVDYV DTFENSDAAR VVSLGNAKCL AESAYNLLQR VVAYDCNMMK QHRTPGTISI YRTAQTAIRC QGEPFVYVRW FRFDAERSLC AFLLSNGAVQ VLVGDQYELR WFDENRKFLL RSNGVCEMVD DSTFALAPDV SRLLYDDF
NT Sequence
atgctcggga gcgtggagac gatatcgtac gacggcgagt ttgttcggct tgtggagctt cgactgccgt tgcgtggctc tacgcagggc cagctgctat caatacggac atgcgaacgc attggtcagg gctcctttgg cacagtttac cgcgctgtgt gtgatggata tcccaaactg gcgctcaaga tcgccaccgg caaggctgca cgtctacgtc aggagctgga cgtgcttgtc aaagtatgca caaaggggaa actgctactg cctcggtttg aatttggtgc tattaacaaa actggcgatt tgattgttgt tggtatggag ctttgcgtgc cgtctacgct gcatgatttg cttctcgtga cacgcctcac caatgaagcc gatatgttat ttcttgcgta tcaggtgttg caggctgttg cctgtgttca cgagcaacaa tgcattcata gagatgttaa gcttcagaat tttgtgttcg atttggatgg caacctgaaa cttatagact ttggattggc atcaagcgca tggaacccac caccgggtga tgtggtggcg ggcaccgtct ccttcatggc tccggaaatg gcacacaacg cactgcacag agatcagcgg gttagcgttg gggcccctgc tgatgtttgg tcggcgggca tcgtgctttt ctctattttc acgcagcgca atccgtatcc accatccatt gcttctatta catcgcctca ttctgatgag gatgaggacg gggatgggga cggcgcggcg gtggctagaa agctcaacag ggagaatttg cggttgctcc gtcgtgtggc cacggcaaag tggcggtggc cggtgggttc tagtgtctcc aacgaactac gccaacttgt ggagttcatt ctcgttaccg atcccgtgaa gcggccggat atctcgacga ttctccgcaa gccaatatgg aatttgcgtc gtcgggcccc gccgaccgcg gtcacggcgt ttttgggtgt tcaagacgaa ttactcttgt cgcacgatga gactcatctt ttgcgagctg tggaacaacg cagcgcggac gtgactgcca gcctgatggg cagccgcgcg aacagcctgg atggggacgg ggacgaagag gcagaacgac gctcatcact aaaatttgtg gcgagggaac aaacagcagt gggcggtgtg ccgacgcatc aggtgtacga tatccgccgc gaatcgaggc agaagagacc ccttcgtgaa atatcggtag tcatcgctga agagacggca aaggggctcc ggcggcgcca gccaaaacgg gcaaggggtg atgtgtcttc ggtttcacgt ggcaattcat gtgcaggttc tcgtataaac tcacacgcga actcgcgcgc cacttctcca gcgaggcagt gtagtgttca tgagtgcgtc caaagtttgg ttaaaggaaa gaaaaatggt aagatgtctc gaaccattcc tcaggcgtcc tcagtgattt ttgacgatga ggaggaagaa ggagtgcaga gagaagtaga acagatggtg actaaaaaag aaaagccttt tgtagcgact cagcgtattc aaaagaaaca gaggaacgga ggagtttgct cccaccaatg cctgcgctct tcccgcattg gcgatgaggc tgaaaacacc aagggccttt tgaggggagg cataaatggg agtgccgtgg atatttcttc aggtgcagtt cttgagctgc ccaaatgtca gatgggacta acgaaggcag ttgacggtga gaccaggacg aaaaaagttc ccatacatgt tcattgcgac cggaaggaac aggtatcggt atggatagat gatatgcagc ttgtcgccat ggagaaatct cagcgagaac ttgcggagaa aaggattttt ttggagctag agtgtctttt ttctgccttg cgcctgacaa cggaagaaaa ccaagcttgg ttcgacatgg tgtggttggc ggaagaacaa cggaagtcgg ctgctcatcc tcatcgtttt aaggagacaa ctcgtgtaag caaaaagtat caatacggct ttgtatgtga catgtgcgat tacgagtttc tgccagctgg taaaaccatc cactttttcc actgcacatg tggcagagat ttatgtgtgg actgtcatcg ggtgtacagc aagcagtgca catgtgcaga atgcggtgcc atctatgaaa atagtgtggc gctgcgtgga catcatcgaa agggcggttg taaagcagta gttaaaaaca gaaggagctc tgtggcttat gaagggcaag taagcgccaa cggacccctt atgcgtagga aaacggcggc acaaacccac gacatgccgc ggggggccac cgtggcaacc ggttcaaaag caacaacaaa agcagcagca gcagcaaaat catttccaag gagtgaggcg gtgaggatgg gagcagtgaa cactgtgaag aaggaggcgg tcgcgaagga gaagaccgca tcgtcgctgt tcagtagacc catcaacacg ccaccagagg ggcagtggaa gccaatggag cgtttgtccg tcagccacaa acccattcat ccaactccgg aagagcgcga ggttttgctg aacggagact ggattcggca ttttcattta tttccgacag aggaagaagc agagccggtt gcctttacgt atcacattca gcctggacgc actggcgcga tttttctcaa tcataaattt tcaatgcatt cggccgtcat ttcagtgctg gagcgacaga tgctcattgt ggactacgtg gacacttttg aaaacagcga tgcggcacgt gttgtttcac tgggaaatgc aaagtgcttg gcagaaagcg catacaacct ccttcaacgc gttgttgcat acgattgtaa catgatgaag caacaccgaa ctcccggcac aatctcaatt taccggacgg cacaaacggc aattcgatgt caaggtgaac cgtttgtgta tgtccgttgg tttcgctttg acgcagagcg ctccttatgt gcgtttttgc tctccaatgg cgcagttcag gtgcttgtag gagatcagta cgaactccgt tggtttgacg aaaatcgaaa gtttctcctc cgtagcaatg gtgtttgtga aatggtggat gattccacct tcgcccttgc tcctgatgtg agccgcctat tgtatgatga tttttaa
Details for TrcrB.18664.b.B2.GE43005
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML GSVETISYDG EFVRLVELRL PLRGSTQGQL LSIRTCERIG QGSFGTVYRA VCDGYPKLAL KIATGKAARL RQELDVLVKV CTKGKLLLPR FEFGAINKTG DLIVVGMELC VPSTLHDLLL VTRLTNEADM LFLAYQVLQA VACVHEQQCI HRDVKLQNFV FDLDGNLKLI DFGLASSAWN PPPGDVVAGT VSFMAPEMAH NALHRDQRVS VGAPADVWSA GIVLFSIFTQ RNPYPPSIAS ITSPHSDEDE DGDGDGAAVA RKLNRENLRL LRRVATAKWR WPVGSSVSNE LRQLVEFILV TDPVKRPDIS TILRKPIW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctcg ggagcgtgga gacgatatcg tacgacggcg agtttgttcg gcttgtggag cttcgactgc cgttgcgtgg ctctacgcag ggccagctgc tatcaatacg gacatgcgaa cgcattggtc agggctcctt tggcacagtt taccgcgctg tgtgtgatgg atatcccaaa ctggcgctca agatcgccac cggcaaggct gcacgtctac gtcaggagct ggacgtgctt gtcaaagtat gcacaaaggg gaaactgcta ctgcctcggt ttgaatttgg tgctattaac aaaactggcg atttgattgt tgttggtatg gagctttgcg tgccgtctac gctgcatgat ttgcttctcg tgacacgcct caccaatgaa gccgatatgt tatttcttgc gtatcaggtg ttgcaggctg ttgcctgtgt tcacgagcaa caatgcattc atagagatgt taagcttcag aattttgtgt tcgatttgga tggcaacctg aaacttatag actttggatt ggcatcaagc gcatggaacc caccaccggg tgatgtggtg gcgggcaccg tctccttcat ggctccggaa atggcacaca acgcactgca cagagatcag cgggttagcg ttggggcccc tgctgatgtt tggtcggcgg gcatcgtgct tttctctatt ttcacgcagc gcaatccgta tccaccatcc attgcttcta ttacatcgcc tcattctgat gaggatgagg acggggatgg ggacggcgcg gcggtggcta gaaagctcaa cagggagaat ttgcggttgc tccgtcgtgt ggccacggca aagtggcggt ggccggtggg ttctagtgtc tccaacgaac tacgccaact tgtggagttc attctcgtta ccgatcccgt gaagcggccg gatatctcga cgattctccg caagccaata tggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat