TrcrB.18664.a

kinetoplastid kinetochore protein 2, putative (kkt2)

CENTER ID: TrcrB.18664.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18664.a.B2.GE43004 PHoranyi kinase domain truncation 99 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.510285.70
UniProt: Q4DIT1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLLEKKQDN SGGSSNSNSS SSGGDVKSRK DNDGKTEEGG EEKDGRLRTP RSYAMLSIPR ELSHTPAIAG LSPTVRTPFI QRCHLHHGDY TSTDNKDEHY RQPLEHMTII FEEDSLQLTT TSLLGKGGFG RVYAARSNGG ELYALKVSAK KLTEGDWARL LKEVELMNHF SRHPNVVKFI AAGRDEETAY VVMECCASRS LHDVIANHGL DVPEILWVGW ALVDTIAFMH SKGCIHRDLK PQNLLFDFDG NLKITDFGLS SRIAEAQPRK TVAGTAMYMA PEIAEAVYKR MSHSTHQPPL QYGQEVDTWS IGVVIYVMLT RMNPYMEAME KRGTREMDKT QKTLSLFNAV ADAAWEWPTG WSGDPELCDL VNWALHRDTK KRATLQEVLQ HPVWNRRPLS CPLSLLYKLN LLERPSSSSV SRHGSKRRSL TRLADNLPIP TGKRTADDVL AEGMRRVVLT EQRARANLVL EHHETLRVML ELLKLSRAEA DVRRSICEDE QRYRTVIQNQ RVTQRSRRRR SEALVSSFSA ATVPTEAGPV TRSSRSHRQS SVVLVAPAAS TKEESGRIVR ASPDKYAMVY PGRDTATRWS LRPVISLPRE LNSEIEEFRC LNHHIMTKLT AMPHGYNGFD CNVCDREILK ISPETPAFRC YKCDYDLCMR CAYQGRLKDV NFVCVSCAKK FTSSAKLQAH SLQCRGPSWS PSPRRSSRMN TMLWEEAEPR RGSLLEVRLP DDPKPRASGR PSGRSSTGRA SSGGRISIGD SHVASPEDVG TMVVQHRDAS FPEMPFTRRA GSGGGRRSTP ARVHGCEDVN SSFSFELPPE IRLSRLKKGE RIQPRTSAEM QEVMDVDSPP LKKERKEARG GGQEKDTPPY KVNESGNIVG IAAQHRAARE ASLQQQQKQQ GQGVIVIRAD AAAKPPEMSR QPQVPRSVSS SRSAGAVSSV AKNVAPKPIE PHLAKVTRTA IPAPTPTNTT APAAPMPNMP KKGRQPSAGG MVTLHGGPPL PRRGPGMPQN TPYLVPQAVP PPNFPVPRQP SHPLVQSGVE ARQVVGGHRV SGGNSAFLAL PREEQNRQRF VDDFLSGGWV RFYSFTNEET VVMYYCAQPG RYGAMFPTEA GVGTAVVDVH SRLVLYVPCM NNESTNRSQP HPHVQTFYDE DVRLLSVSEA QRHLGGVLES IMGFVNEVAR LRAEGLTPAA VHAAYIHQRD KSSVPKDTKF VYVRKVFPDP SGSFTLFRLS NLRSQVVCNT LMDIRWQSDR RHNVGQKYYV LADGTAEPFV VDHTGILTQV ETVLSNNFRR
NT Sequence
atgtcattac ttgaaaagaa gcaggataac agtggcggca gcagcaatag taatagcagt agtagcggtg gtgatgtcaa atccaggaag gataatgatg gaaaaacaga ggagggggga gaagaaaagg atggacggct acggacaccc agatcgtacg ccatgttatc tattccacgt gaactgtcac atacacctgc cattgctgga ttgtctccta ctgttcgaac gccatttatt cagagatgcc atttgcatca cggtgactat accagcaccg acaacaagga tgagcattat cggcagccgt tggagcatat gacaatcata tttgaggagg atagcctgca gctcacgacg acaagtttgt taggtaaagg gggttttggt cgtgtgtatg cggcgcggtc caatgggggt gagttatatg ccttgaaggt atccgcaaag aaactgactg agggtgattg ggcccgtctt ctgaaggagg tggaactgat gaatcacttc tcacggcacc ccaacgtggt gaagtttatc gccgccggac gggatgagga gacggcgtat gtggtgatgg agtgctgcgc cagccgctca ctgcacgatg ttatcgccaa tcacggacta gatgtaccgg agattctgtg ggtggggtgg gctcttgtgg acaccatcgc cttcatgcat tctaagggtt gcatccaccg tgacctgaaa ccgcagaatc ttctttttga ctttgatggg aatttgaaaa ttacagactt tggcctttcc agtagaattg cggaggcgca accacgcaag acagttgcgg gcaccgctat gtacatggcg ccagagattg cggaggcagt ttacaagcgt atgagtcatt caacccatca gccgccgctt cagtacggtc aagaagtgga tacatggagt attggtgtcg tgatttacgt catgctgaca cgcatgaacc catacatgga ggcaatggag aagagaggga cacgggaaat ggataaaacg caaaaaactc tctcactctt taatgctgtt gcggatgccg catgggagtg gccaacaggg tggtcaggcg acccggaact ctgcgatctt gtaaactggg cacttcatcg ggataccaag aagcgtgcca cgctgcagga ggtgttgcaa caccccgtgt ggaatcgacg tccgctgtca tgcccactct ctctgttgta caagttgaat ctgctggaac gtccctcttc ctcttctgtt tcacgccatg gctccaaacg ccgttcattg actcgtctcg cggacaatct tccaattccc acaggaaaga ggacggcaga tgatgtgctc gcggagggaa tgcggcgcgt cgtattgacg gaacaaaggg ctcgtgcaaa tttggtgttg gagcatcacg aaacgttacg tgtcatgctg gagttgttga aactttcacg tgcggaggcg gacgtcagac ggagcatttg tgaggacgaa cagagatacc gcactgtgat tcaaaatcag cgggtcacac agagaagcag acgcagaagg agtgaggcac tcgtatcgtc attttccgcg gcaaccgtac cgacagaggc gggacccgtg actcgctcct cgcgctcgca cagacagtct agcgtcgttc tggtggcgcc tgcggcttcc acgaaggagg aatccggaag aattgtccgt gcctcacccg acaagtacgc catggtttat ccaggacgag acaccgctac acggtggtcg ttgcgtcccg tgatttcact ccctcgtgaa ctaaacagcg aaatagaaga gtttaggtgc ctgaaccatc acatcatgac aaaattgaca gctatgcctc atggatacaa tgggtttgat tgtaatgtgt gtgatcgtga gattcttaag atttctcctg aaactcctgc cttccgttgc tataaatgcg actacgacct ttgtatgcga tgtgcctatc aagggcgtct caaggacgtt aattttgtgt gcgtgtcatg cgcgaaaaag tttacttctt ctgcaaaatt gcaggcccac tcgttacagt gccgtggtcc tagctggagt ccatccccac gccgttcctc acgtatgaac acgatgctgt gggaggaggc cgaaccacga cgtggcagcc tacttgaggt gcgacttccc gatgatccaa agccccgagc cagtggacgt ccatctgggc gtagttcaac aggacgagcc tcctctggcg gacgcatcag catcggcgac agccatgttg cctctccaga agatgtggga accatggtgg tgcagcatcg tgatgcatcc tttccagaga tgccctttac acggcgggcg ggaagcggtg gcggacgaag gtcaacgccg gctagagttc acgggtgtga ggatgtcaac agcagttttt catttgagct tccacctgaa attcgtctgt caagacttaa gaaaggggag aggattcaac caagaacttc tgcggagatg caggaggtga tggatgtgga ctcccctcca ctaaagaaag aacggaaaga ggcgcgcggc gggggacagg agaaagatac gcctccttac aaagtgaatg aaagtgggaa tattgttggc atagccgctc agcaccgagc cgcgagagaa gctagtcttc aacagcagca gaaacaacaa ggacaaggtg tcattgttat tagagccgac gcggccgcca aaccaccaga gatgtctcga caaccccagg taccgcgaag tgtttcgtcc agccgttccg caggggcggt gtcgtcggtc gcaaagaacg tggcccccaa accaattgaa cctcacttgg caaaggtcac gcgaacagct atccctgctc ccactcccac aaatactacg gcccctgctg ctccaatgcc aaacatgcca aagaagggac gtcagccctc tgcgggtggc atggtgacgc tgcatggtgg gcccccgtta ccccgtagag ggccgggcat gccacagaac acaccatact tggtgcccca agccgttcca ccgccgaatt ttcccgtacc gcgtcagcca tcccatccgt tggtgcaatc tggcgtcgaa gcacgccaag ttgttggcgg ccatcgtgtt tctggcggca acagtgcatt tttggcactt cctcgtgaag aacagaatag acagcggttt gttgacgact tccttagtgg tggatgggtt cgtttctact ccttcaccaa tgaagagacg gtagtgatgt attactgcgc acagcccggt agatatggcg ccatgtttcc aacagaagcc ggtgtgggca cggcggtagt ggatgttcac tctagactag tgttgtacgt accatgcatg aacaacgaga gcacaaaccg cagtcaacca cacccacatg tgcagacttt ttacgatgag gatgtaagac tactcagcgt ctccgaggca caacgacatc ttggcggtgt tctagaaagt atcatgggtt ttgtgaatga agtggcacga cttcgggcgg aaggattgac gccggcggcc gttcacgcag catatattca tcaacgtgat aaaagctccg tgccaaagga cacaaagttt gtctatgtaa gaaaggtgtt cccagaccct tccggctctt tcacactctt tcggctctct aacctccgct cacaggtagt gtgcaacacg ctcatggata ttcgctggca aagtgacaga agacacaacg ttgggcaaaa gtactacgtt ctcgcagacg gtacggcaga accgttcgtt gtggatcata ccggaattct tacgcaagtg gagacggtac tgagcaacaa tttccgtcga tga
Details for TrcrB.18664.a.B2.GE43004
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHHY RQPLEHMTII FEEDSLQLTT TSLLGKGGFG RVYAARSNGG ELYALKVSAK KLTEGDWARL LKEVELMNHF SRHPNVVKFI AAGRDEETAY VVMECCASRS LHDVIANHGL DVPEILWVGW ALVDTIAFMH SKGCIHRDLK PQNLLFDFDG NLKITDFGLS SRIAEAQPRK TVAGTAMYMA PEIAEAVYKR MSHSTHQPPL QYGQEVDTWS IGVVIYVMLT RMNPYMEAME KRGTREMDKT QKTLSLFNAV ADAAWEWPTG WSGDPELCDL VNWALHRDTK KRATLQEVLQ HPV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcattatc ggcagccgtt ggagcatatg acaatcatat ttgaggagga tagcctgcag ctcacgacga caagtttgtt aggtaaaggg ggttttggtc gtgtgtatgc ggcgcggtcc aatgggggtg agttatatgc cttgaaggta tccgcaaaga aactgactga gggtgattgg gcccgtcttc tgaaggaggt ggaactgatg aatcacttct cacggcaccc caacgtggtg aagtttatcg ccgccggacg ggatgaggag acggcgtatg tggtgatgga gtgctgcgcc agccgctcac tgcacgatgt tatcgccaat cacggactag atgtaccgga gattctgtgg gtggggtggg ctcttgtgga caccatcgcc ttcatgcatt ctaagggttg catccaccgt gacctgaaac cgcagaatct tctttttgac tttgatggga atttgaaaat tacagacttt ggcctttcca gtagaattgc ggaggcgcaa ccacgcaaga cagttgcggg caccgctatg tacatggcgc cagagattgc ggaggcagtt tacaagcgta tgagtcattc aacccatcag ccgccgcttc agtacggtca agaagtggat acatggagta ttggtgtcgt gatttacgtc atgctgacac gcatgaaccc atacatggag gcaatggaga agagagggac acgggaaatg gataaaacgc aaaaaactct ctcactcttt aatgctgttg cggatgccgc atgggagtgg ccaacagggt ggtcaggcga cccggaactc tgcgatcttg taaactgggc acttcatcgg gataccaaga agcgtgccac gctgcaggag gtgttgcaac accccgtgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat