SSGCID: Target Page

TrcrB.18445.b

tyrosyl or methionyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18445.b
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18445.b.B1.GE42639	full length		1	243

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.511217.200
UniProt:	Q4DVC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFSSNFEGPL LRRSIPFVER WIAELVAARE ASLGATKKNA TPEESPQAVG VKGTKKDGRA ATVAAAAAEK EEASGSSMSK CHFVIGKVLD VRPHPESTKL YIEEIELGEQ AGGRRTILSG LQEYVKQDEF INRLVLVIAN LEPRKIGGIV SEGMVLCASR TEGNTRDVKL LEVPAGTPVG ERVVFDGHDG PFVPVLKKKL AKHFEKVAVD LKTNEKGEVM WGDMPFRTSH GIITAPIPNG VVS

NT Sequence

atgttctctt caaattttga gggtcccctt ctgcgccgct ccatcccatt cgtggagcgc tggatcgccg agcttgtcgc cgcaagagaa gcgtccctcg gcgccacgaa gaagaatgca acaccggaag aatccccaca agcggtcggc gtaaagggaa cgaaaaagga cgggagggct gccactgtcg ctgctgctgc tgcggagaag gaggaggcat ctggcagcag tatgtccaag tgtcactttg tgatcggcaa ggtacttgat gtccgtccac acccagagag cacgaagtta tacatcgagg aaattgagct tggcgagcag gcggggggac gtcgcaccat ccttagcggc ttgcaagagt acgttaagca ggacgagttt ataaaccgtc tggtgcttgt cattgcgaat ctggagccgc gaaagatcgg cggtattgtt tcagaaggca tggtgctttg cgcctcgcgt acagagggca acacgcggga tgtcaagtta ttggaagtgc cagccggtac gcccgtcggc gaacgtgttg tgtttgacgg tcacgacggg ccgtttgtgc cggtgctgaa gaagaagttg gcgaaacact ttgaaaaggt ggcggttgac ctcaagacga atgaaaaggg tgaggtaatg tggggtgaca tgccgtttcg tacgagccac ggcatcatta cggcacccat accgaatggg gtcgtgtcat ga

Details for TrcrB.18445.b.B1.GE42639

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF SSNFEGPLLR RSIPFVERWI AELVAAREAS LGATKKNATP EESPQAVGVK GTKKDGRAAT VAAAAAEKEE ASGSSMSKCH FVIGKVLDVR PHPESTKLYI EEIELGEQAG GRRTILSGLQ EYVKQDEFIN RLVLVIANLE PRKIGGIVSE GMVLCASRTE GNTRDVKLLE VPAGTPVGER VVFDGHDGPF VPVLKKKLAK HFEKVAVDLK TNEKGEVMWG DMPFRTSHGI ITAPIPNGVV S

Validated NT Sequence

atcttactca cttatgacac gaccccattc ggtatgggtg ccgtaatgat gccgtggctc gtacgaaacg gcatgtcacc ccacattacc tcaccctttt cattcgtctt gaggtcaacc gccacctttt caaagtgttt cgccaacttc ttcttcagca ccggcacaaa cggcccgtcg tgaccgtcaa acacaacacg ttcgccgacg ggcgtaccgg ctggcacttc caataacttg acatcccgcg tgttgccctc tgtacgcgag gcgcaaagca ccatgccttc tgaaacaata ccgccgatct ttcgcggctc cagattcgca atgacaagca ccagacggtt tataaactcg tcctgcttaa cgtactcttg caagccgcta aggatggtgc gacgtccccc cgcctgctcg ccaagctcaa tttcctcgat gtataacttc gtgctctctg ggtgtggacg gacatcaagt accttgccga tcacaaagtg acacttggac atactgctgc cagatgcctc ctccttctcc gcagcagcag cagcgacagt ggcagccctc ccgtcctttt tcgttccctt tacgccgacc gcttgtgggg attcttccgg tgttgcattc ttcttcgtgg cgccgaggga cgcttctctt gcggcgacaa gctcggcgat ccagcgctcc acgaatggga tggagcggcg cagaagggga ccctcaaaat ttgaagagaa catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttct cttcaaattt tgagggtccc cttctgcgcc gctccatccc attcgtggag cgctggatcg ccgagcttgt cgccgcaaga gaagcgtccc tcggcgccac gaagaagaat gcaacaccgg aagaatcccc acaagcggtc ggcgtaaagg gaacgaaaaa ggacgggagg gctgccactg tcgctgctgc tgctgcggag aaggaggagg catctggcag cagtatgtcc aagtgtcact ttgtgatcgg caaggtactt gatgtccgtc cacacccaga gagcacgaag ttatacatcg aggaaattga gcttggcgag caggcggggg gacgtcgcac catccttagc ggcttgcaag agtacgttaa gcaggacgag tttataaacc gtctggtgct tgtcattgcg aatctggagc cgcgaaagat cggcggtatt gtttcagaag gcatggtgct ttgcgcctcg cgtacagagg gcaacacgcg ggatgtcaag ttattggaag tgccagccgg tacgcccgtc ggcgaacgtg ttgtgtttga cggtcacgac gggccgtttg tgccggtgct gaagaagaag ttggcgaaac actttgaaaa ggtggcggtt gacctcaaga cgaatgaaaa gggtgaggta atgtggggtg acatgccgtt tcgtacgagc cacggcatca ttacggcacc cataccgaat ggggtcgtgt catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat