TrcrB.18370.a

Phosphatidylinositol 4-kinase, putative (Pi4K)

CENTER ID: TrcrB.18370.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18370.a.B1.GE42096 full length 1 584
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tc00.1047053508695.20
UniProt: Q4CMW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKASSNLSLR RRELLERLLD LSTATFDEQV LIVNKLYDTP LPTLEEVLLQ VSHVCITHGE AEIQDLLRNF MLWIAAQSFS AALRLSWTVD SVQKIFGLLG LGDRVKEIRD RIESFAINRR GNVRDGNDDE VTEAEIMRKE LRLKLYNEER QFISIVTHLS SQLCQHPDKR KRQTELKRKL QEINNSLSTQ SIIYPFGGVA DPVKWIVNIA VDECVVFSSR ERAPFLLRCE VILDDTTSMG DPVASKLRLP NGSFKIAADF DEVFTPSYAQ EGSGDEKDMK TDGVFQGSDR DAFVCAFGES AEECVARMRK KSVFGKHPNW SVVSVVVKAK DDLRQEELAL QLIDIFNKIW EEGGITCSVR PHRALSVSAD SGIMEYLDGA CSIDGIKKLC QVSSLAQFFN EAFGPVGSER HSKAEMNFVE TMAGYSIVSY MLQVKDRHNG NLMISSEGHL VHIDFGFMFA TSPGGINFES APFKLSQELM EVMGGVGSAA FNYFKLLLYQ GMVAVRERAD DILGLVSLMT PYNTMSCFGS DPASTVQQLR SRFRFDLETE TDFALHVKEL ILGSVDNWRT RRYDQFQTLQ NGIL
NT Sequence
atgaaggcgt cgtcgaattt gtcgctgcgg cgacgtgagc tactggagcg gttacttgac ctctctacag ccacatttga cgaacaggtt ctgatcgtca acaaattgta tgacaccccg ctcccgacgc tggaggaggt cctgctgcag gtgtctcatg tttgcatcac tcacggcgag gcggagattc aggacctttt gcgcaacttt atgctctgga tagctgctca gtcgttcagc gccgcgctgc gattgtcgtg gacagtggac tccgtgcaga agatatttgg attgttgggg ctcggtgacc gcgtgaagga gattcgggac agaattgaga gcttcgcgat taaccgcaga gggaatgtta gagacgggaa tgatgacgaa gtgacagagg cagagattat gcggaaggaa ttgcggctca agttatacaa tgaagagcga caatttatca gcatcgtcac acacttgagc tcccagctgt gccaacatcc ggataagcga aagcgccaaa cagaattaaa aagaaagcta caagaaatca acaattcatt gagcacccag tccatcatct atcctttcgg gggtgtcgcg gatcccgtca agtggattgt gaacatagcc gtggatgaat gtgttgtctt ctcttctcgt gaaagggcac cctttttgct tcgctgtgaa gtcattcttg acgacacaac gtcgatgggt gacccggtgg catcaaaatt gcgtttaccc aatgggtcgt ttaaaattgc ggctgatttc gatgaggtgt ttacaccgtc atatgcccaa gaggggtcag gtgacgagaa agacatgaaa actgatgggg tatttcaagg tagcgatcgg gacgcatttg tatgcgcgtt tggtgagtca gcagaagagt gtgttgcacg tatgcgaaaa aaatccgtct ttgggaagca cccaaactgg agtgtagttt ccgttgttgt taaggcaaag gatgatctgc gacaggagga attggccttg cagctaatcg atatttttaa caaaatttgg gaggaaggtg gaattacatg ctccgttcgt ccccatcgag ctctttctgt ttccgcagat tcggggatta tggaatactt ggatggtgcc tgctcgatcg acggcatcaa aaaattgtgc caagtgagca gccttgcgca attttttaat gaagcttttg ggccagtggg ttccgaaagg cattccaaag ctgaaatgaa ttttgtagag acgatggcag ggtacagcat tgtttcctac atgcttcagg tcaaagatag acataacggc aatcttatga ttagcagtga gggacacttg gtgcatatcg actttgggtt tatgtttgct acgtcacctg gtgggataaa ttttgaatct gcccccttta agctttcgca ggagcttatg gaagtgatgg gaggtgttgg aagtgcagcg ttcaattact tcaagttgtt gctttatcag ggaatggtgg cggtacggga gcgtgcagac gatattttag gccttgtgtc gctcatgact ccttacaaca ccatgtcttg ctttggcagt gatcccgcaa gtaccgttca acagctgcgc tctcgttttc gctttgactt ggagaccgag acggattttg ctctccatgt aaaagaactc attctgggaa gcgtcgataa ttggcgaacg aggcgatacg atcagtttca aacccttcag aatggtatct tgtga
Details for TrcrB.18370.a.B1.GE42096
HARVESTED ON: data unavailable
SEQUENCED ON: 12/7/2017
EXPECTED MW: 67kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMK ASSNLSLRRR ELLERLLDLS TATFDEQVLI VNKLYDTPLP TLEEVLLQVS HVCITHGEAE IQDLLRNFML WIAAQSFSAA LRLSWTVDSV QKIFGLLGLG DRVKEIRDRI ESFAINRRGN VRDGNDDEVT EAEIMRKELR LKLYNEERQF ISIVTHLSSQ LCQHPDKRKR QTELKRKLQE INNSLSTQSI IYPFGGVADP XXWIVNIAVD ECVVFSSREX XXFLLRCXXI LDXXTXXGXX XASXXXXXXX RXXLRXXXXX XXXXXXXXXX XXXXXXXXXX XXFQGSDRXA FVXXXXESAE ECXXXMRKKS VFGKHPNWNV XXVVVKAKDD LRQEELALQL IDIFNKIWEE GGITCSVRPH RALSVSADSG IMEYLDGACS IDGIKKLCQV SSLAQFFNEA FGPVGSERHS KAEMNFVETM AGYSIVSYML QVKDRHNGNL MISSEGHLVH IDFGFMFATS PGGINFESAP FKLSQELMEV MGGVGSAAFN YFKLLLYQGM VAVRERADDI LGLVSLMTPY NTMSCFGSDP ASTVQQLRSR FRFDLETETD FALHVKELIL GSVDNWRTRR YDQFQ
Validated NT Sequence
tttgaaactg atcgtatcgc ctcgttcgcc aattatcgac gcttcccaga atgagttctt ttacatggag agcaaaatcc gtctcggtct ccaagtcaaa gcgaaaacga gagcgcagct gttgaacggt acttgcggga tcactgccaa agcaagacat ggtgttgtaa ggagtcatga gcgacacaag gcctaaaata tcgtctgcac gctcccgtac cgccaccatt ccctgataaa gcaacaactt gaagtaattg aacgctgcac ttccaacacc tcccatcact tccataagct cctgcgaaag cttaaagggg gcagattcaa aatttatccc accaggtgac gtagcaaaca taaacccaaa gtcgatatgc accaagtgtc cctcactgct aatcataaga ttgccgttat gtctatcttt gacctgaagc atgtaggaaa caatgctgta ccctgccatc gtctctacaa aattcatttc agctttggaa tgcctttcgg aacccactgg cccaaaagct tcattaaaaa attgcgcaag gctgctcact tggcacaatt ttttgatgcc gtcgatcgag caggcaccat ccaagtattc cataatcccc gaatctgcgg aaacagaaag agctcgatga ggacgaacgg agcatgtaat tccaccttcc tcccaaattt tgttaaaaat atcgattagc tgcaaggcca attcctcctg tcgcagatca tcctttgcct taacaacaac nnnnnntaca ttccagtttg ggtgcttccc aaagacggat ttttttcgca tnnnnnnnnc acactcttct gctgactcnn nnnnnnnnnn nacaaaggcg ncccgatcgc taccttgaaa tnnnnnntnn nnnnnnnnnn nnnnnnnnnc nnnnnnnnnn tnnnnnnnnn nnnnnnnnnn nnaannnnnn nnnnnnnnnn ntcgcaatnn nnnacgnnnc nnnnnnnnnn gnaannntga tgccannnnn nnacccannn acgttnnnnc gtcaagaatn nnnnnacagc gaagcagaaa ggnnnnnntt tcacgagaag agaagacaac acattcatcc acggctatgt tcacaatcca nnnnncggga tccgcgacac ccccgaaagg atagatgatg gactgggtgc tcaatgaatt gttgatttct tgtagctttc tttttaattc tgtttggcgc tttcgcttat ccggatgttg gcacagctgg gagctcaagt gtgtgacgat gctgataaat tgtcgctctt cattgtataa cttgagccgc aattccttcc gcataatctc tgcctctgtc acttcgtcat cattcccgtc tctaacattc cctctgcggt taatcgcgaa gctctcaatt ctgtcccgaa tctccttcac gcggtcaccg agccccaaca atccaaatat cttctgcacg gagtccactg tccacgacaa tcgcagcgcg gcgctgaacg actgagcagc tatccagagc ataaagttgc gcaaaaggtc ctgaatctcc gcctcgccgt gagtgatgca aacatgagac acctgcagca ggacctcctc cagcgtcggg agcggggtgt catacaattt gttgacgatc agaacctgtt cgtcaaatgt ggctgtagag aggtcaagta accgctccag tagctcacgt cgccgcagcg acaaattcga cgacgccttc atatggtggt ggtggtggtg agccatggta tat
Expected Protein Sequence
MAHHHHHHMK ASSNLSLRRR ELLERLLDLS TATFDEQVLI VNKLYDTPLP TLEEVLLQVS HVCITHGEAE IQDLLRNFML WIAAQSFSAA LRLSWTVDSV QKIFGLLGLG DRVKEIRDRI ESFAINRRGN VRDGNDDEVT EAEIMRKELR LKLYNEERQF ISIVTHLSSQ LCQHPDKRKR QTELKRKLQE INNSLSTQSI IYPFGGVADP VKWIVNIAVD ECVVFSSRER APFLLRCEVI LDDTTSMGDP VASKLRLPNG SFKIAADFDE VFTPSYAQEG SGDEKDMKTD GVFQGSDRDA FVCAFGESAE ECVARMRKKS VFGKHPNWSV VSVVVKAKDD LRQEELALQL IDIFNKIWEE GGITCSVRPH RALSVSADSG IMEYLDGACS IDGIKKLCQV SSLAQFFNEA FGPVGSERHS KAEMNFVETM AGYSIVSYML QVKDRHNGNL MISSEGHLVH IDFGFMFATS PGGINFESAP FKLSQELMEV MGGVGSAAFN YFKLLLYQGM VAVRERADDI LGLVSLMTPY NTMSCFGSDP ASTVQQLRSR FRFDLETETD FALHVKELIL GSVDNWRTRR YDQFQTLQNG IL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagg cgtcgtcgaa tttgtcgctg cggcgacgtg agctactgga gcggttactt gacctctcta cagccacatt tgacgaacag gttctgatcg tcaacaaatt gtatgacacc ccgctcccga cgctggagga ggtcctgctg caggtgtctc atgtttgcat cactcacggc gaggcggaga ttcaggacct tttgcgcaac tttatgctct ggatagctgc tcagtcgttc agcgccgcgc tgcgattgtc gtggacagtg gactccgtgc agaagatatt tggattgttg gggctcggtg accgcgtgaa ggagattcgg gacagaattg agagcttcgc gattaaccgc agagggaatg ttagagacgg gaatgatgac gaagtgacag aggcagagat tatgcggaag gaattgcggc tcaagttata caatgaagag cgacaattta tcagcatcgt cacacacttg agctcccagc tgtgccaaca tccggataag cgaaagcgcc aaacagaatt aaaaagaaag ctacaagaaa tcaacaattc attgagcacc cagtccatca tctatccttt cgggggtgtc gcggatcccg tcaagtggat tgtgaacata gccgtggatg aatgtgttgt cttctcttct cgtgaaaggg cacccttttt gcttcgctgt gaagtcattc ttgacgacac aacgtcgatg ggtgacccgg tggcatcaaa attgcgttta cccaatgggt cgtttaaaat tgcggctgat ttcgatgagg tgtttacacc gtcatatgcc caagaggggt caggtgacga gaaagacatg aaaactgatg gggtatttca aggtagcgat cgggacgcat ttgtatgcgc gtttggtgag tcagcagaag agtgtgttgc acgtatgcga aaaaaatccg tctttgggaa gcacccaaac tggagtgtag tttccgttgt tgttaaggca aaggatgatc tgcgacagga ggaattggcc ttgcagctaa tcgatatttt taacaaaatt tgggaggaag gtggaattac atgctccgtt cgtccccatc gagctctttc tgtttccgca gattcgggga ttatggaata cttggatggt gcctgctcga tcgacggcat caaaaaattg tgccaagtga gcagccttgc gcaatttttt aatgaagctt ttgggccagt gggttccgaa aggcattcca aagctgaaat gaattttgta gagacgatgg cagggtacag cattgtttcc tacatgcttc aggtcaaaga tagacataac ggcaatctta tgattagcag tgagggacac ttggtgcata tcgactttgg gtttatgttt gctacgtcac ctggtgggat aaattttgaa tctgccccct ttaagctttc gcaggagctt atggaagtga tgggaggtgt tggaagtgca gcgttcaatt acttcaagtt gttgctttat cagggaatgg tggcggtacg ggagcgtgca gacgatattt taggccttgt gtcgctcatg actccttaca acaccatgtc ttgctttggc agtgatcccg caagtaccgt tcaacagctg cgctctcgtt ttcgctttga cttggagacc gagacggatt ttgctctcca tgtaaaagaa ctcattctgg gaagcgtcga taattggcga acgaggcgat acgatcagtt tcaaaccctt cagaatggta tcttgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat