TrcrB.18331.c

Fumarate hydratase, putative (class I)

CENTER ID: TrcrB.18331.c
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18331.c.B1.GE42094 full length 1 555
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tc00.1047053507669.10
UniProt: Q4DRX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRRSAAALG KKAPVPFKYV PLIPHVSHDD TPLRLLTKDY VSVVRPGCGV PEILQVDVEG LTRLASEAFG DVQHLLRPSH LASLRKIFDD PEASDNDRFV ALQLLKNANI AAARVLPGCQ DTGTAIVAGY KGEQVFTNGD ECEALSRGVY KIYTTTNLRY SQNVPLTMFD EKNTGSNLPA QIDLYATKGQ EYNFMFVAKG GGSANKAYLF QETKSVLNPK SLRKFLEEKI GAIGTAACPP YHMAVVVGGT SAEMTLKTVK YASCKYYDNL PTKPDESKGY AYRDTEMENV VMDICYNMGM GAQFGGKYFA HDARVIRLPR HGASCPIGIG VSCSADRQAL AKINKDGIWL EQLETDPAKY LPEITEDQLL KTPPVNIDLS MPMEKIRAEL SKYPVKTRLS LSGTIIVARD MAHARMREML EAGKPLPEYI KNHPVYYAGP AKCPEGMPSG SFGPTTAGRM DPFVDLFQAN GGSFVMLAKG NRSRAVTQAC KKHGGFYLGS IGGPAALLAK DSIRKVEVLD MAELGMEAVW KIEVENFPAF IVLDDKGNDF FQQLK
NT Sequence
atgctgcgcc gttctgcggc agcccttggc aagaaggccc cggtgccatt caagtacgtc ccgcttattc cgcacgtctc ccacgacgac acgccgcttc gtctcttgac gaaggactac gtttctgttg tgaggccggg ctgtggcgtg ccggagatcc tgcaggtcga tgtggaggga ctgacgaggc tcgcctcgga ggcttttggc gatgtgcagc atcttcttcg cccatcccac ctcgcgagtc tgcggaagat tttcgacgac ccggaggcga gcgacaacga tcgtttcgtc gcgctacagc ttctaaagaa tgccaacatt gcggcagctc gcgtccttcc cgggtgccag gacacaggca cggcgattgt tgccggctac aagggcgaac aggtgttcac aaacggcgat gaatgcgagg cgttgagtcg tggcgtctac aagatttaca ccacaacaaa tctacgttac agtcaaaatg ttcctctcac catgtttgat gaaaaaaaca cgggctccaa tctgccggca cagattgact tgtatgcgac gaagggacaa gagtacaact tcatgtttgt ggcaaaagga ggcggctcgg cgaacaaggc gtatcttttc caggagacga agtcggttct taacccaaag tccctccgta agtttttaga ggaaaaaatt ggcgctattg gtaccgcggc ctgcccacca taccatatgg ctgtcgtcgt gggtggcaca agcgccgaaa tgacactcaa gacagtgaag tacgcatctt gcaaatacta cgacaatcta cccacaaaac cggatgagtc caagggctac gcctaccgcg acactgaaat ggagaatgtc gttatggaca tctgctacaa catgggcatg ggggcccagt ttggggggaa gtactttgcc catgatgccc gcgtcattcg cctgccccgt cacggcgcca gttgtccgat aggcatcggt gtaagctgca gcgccgatcg tcaggcactt gcaaaaataa acaaggacgg tatttggctt gagcagctcg aaacagatcc cgcaaaatac ctcccagaga tcaccgaaga ccagctcctt aagacaccac ccgtgaatat tgacctaagt atgcctatgg agaagattcg cgctgaactt tcaaagtatc ctgtcaagac acgactaagt ttgagtggca ccatcattgt tgcccgagac atggcccatg cacggatgcg ggaaatgctt gaggcgggaa agccgttacc agaatacatt aagaaccacc ccgtgtacta cgcagggccg gcaaaatgtc ctgaaggcat gccttctggt tcctttggtc caacgacagc gggacgcatg gacccatttg tggatctctt tcaggcaaac ggcggttcct ttgttatgct cgcaaaagga aaccgcagtc gagcagtaac gcaggcatgt aagaaacacg gaggattcta tcttggcagt atcggtggcc ctgccgcact gcttgcaaaa gactccattc gcaaggtaga ggtgcttgat atggcggagt tgggaatgga ggcggtgtgg aagattgaag tggagaattt tcccgccttc attgttttgg acgacaaggg aaatgacttc ttccaacagc tcaaatga
Details for TrcrB.18331.c.B1.GE42094
HARVESTED ON: data unavailable
SEQUENCED ON: 12/7/2017
EXPECTED MW: 62kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 50
Validated AA Sequence
MAHHHHHHML RRSAAALGKK APVPFKYVPL IPHVSHDDTP LRLLTKDYVS VVRPGCGVPE ILQVDVEGLT RLASEAFGDV QHLLRPSHLA SLRKIFDDPE ASDNDRFVAL QLLKNANIAA ARVLPGCQDT GTAIVAGYKG EQVFTNGDEC EALSRGVYKI YTTTNLRYSQ NVPLTMFDEK NTGSNLPAQI DLYATKGQEY NFMFVAKGGG SANKAYLFQE TKSVLNPKSL RKFLEEKIGA IGTAACPPYH MAVVVGGTSA EMTLKTVKYA SCKYYDNLPT XXG
Validated NT Sequence
catttccctt gtcgtccaaa acaatgaagg cgggaaaatt ctccacttca atcttccaca ccgcctccat tcccaactcc gccatatcaa gcacctctac cttgcgaatg gagtcttttg caagcagtgc ggcagggcca ccgatactgc caagatagaa tcctccgtgt ttcttacatg cctgcgttac tgctcgactg cggtttcctt ttgcgagcat aacaaaggaa ccgccgtttg cctgaaagag atccacaaat gggtccatgc gtcccgctgt cgttggacca aaggaaccag aaggcatgcc ttcaggacat tttgccggcc ctgcgtagta cacggggtgg ttcttaatgt attctggtaa cggctttccc gcctcaagca tttcccgcat ccgtgcatgg gccatgtctc gggcaacaat gatggtgcca ctcaaactta gtcgtgtctt gacaggatac tttgaaagtt cagcgcgaat cttctccata ggcatactta ggtcaatatt cacgggtggt gtcttaagga gctggtcttc ggtgatctct gggaggtatt ttgcgggatc tgtttcgagc tgctcaagcc aaataccgtc cttgtttatt tttgcaagtg cctgacgatc ggcgctgcag cttacaccga tgcctatcgg acaactggcg ccgtgacggg gcaggcgaat gacgcgggca tcatgggcaa agtacttccc ccnnnaactg ggcccccatg cccatgttgt agcagatgtc cataacgaca ttctccattt cagtgtcgcg gtaggcgtag cccttggact catccnnnnt ttgtgggtag attgtcgtag tatttgcaag atgcgtactt cactgtcttg agtgtcattt cggcgcttgt gccacccacg acgacagcca tatggtatgg tgggcaggcc gcggtaccaa tagcgccaat tttttcctct aaaaatttac ggagggactt tgggttaaga accgacttcg tctcctggaa aagatacgcc ttgttcgccg agccgcctcc ttttgccaca aacatgaagt tgtactcttg tcccttcgtc gcatacaagt caatctgtgc cggcagattg gagcccgtgt ttttttcatc aaacatggtg agaggaacat tttgactgta acgtagattt gttgtggtgt aaatcttgta gacgccacga ctcaacgcct cgcattcatc gccgtttgtg aacacctgtt cgcccttgta gccggcaaca atcgccgtgc ctgtgtcctg gcacccggga aggacgcgag ctgccgcaat gttggcattc tttagaagct gtagcgcgac gaaacgatcg ttgtcgctcg cctccgggtc gtcgaaaatc ttccgcagac tcgcgaggtg ggatgggcga agaagatgct gcacatcgcc aaaagcctcc gaggcgagcc tcgtcagtcc ctccacatcg acctgcagga tctccggcac gccacagccc ggcctcacaa cagaaacgta gtccttcgtc aagagacgaa gcggcgtgtc gtcgtgggag acgtgcggaa taagcgggac gtacttgaat ggcaccgggg ccttcttgcc aagggctgcc gcagaacggc gcagcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHML RRSAAALGKK APVPFKYVPL IPHVSHDDTP LRLLTKDYVS VVRPGCGVPE ILQVDVEGLT RLASEAFGDV QHLLRPSHLA SLRKIFDDPE ASDNDRFVAL QLLKNANIAA ARVLPGCQDT GTAIVAGYKG EQVFTNGDEC EALSRGVYKI YTTTNLRYSQ NVPLTMFDEK NTGSNLPAQI DLYATKGQEY NFMFVAKGGG SANKAYLFQE TKSVLNPKSL RKFLEEKIGA IGTAACPPYH MAVVVGGTSA EMTLKTVKYA SCKYYDNLPT KPDESKGYAY RDTEMENVVM DICYNMGMGA QFGGKYFAHD ARVIRLPRHG ASCPIGIGVS CSADRQALAK INKDGIWLEQ LETDPAKYLP EITEDQLLKT PPVNIDLSMP MEKIRAELSK YPVKTRLSLS GTIIVARDMA HARMREMLEA GKPLPEYIKN HPVYYAGPAK CPEGMPSGSF GPTTAGRMDP FVDLFQANGG SFVMLAKGNR SRAVTQACKK HGGFYLGSIG GPAALLAKDS IRKVEVLDMA ELGMEAVWKI EVENFPAFIV LDDKGNDFFQ QLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gccgttctgc ggcagccctt ggcaagaagg ccccggtgcc attcaagtac gtcccgctta ttccgcacgt ctcccacgac gacacgccgc ttcgtctctt gacgaaggac tacgtttctg ttgtgaggcc gggctgtggc gtgccggaga tcctgcaggt cgatgtggag ggactgacga ggctcgcctc ggaggctttt ggcgatgtgc agcatcttct tcgcccatcc cacctcgcga gtctgcggaa gattttcgac gacccggagg cgagcgacaa cgatcgtttc gtcgcgctac agcttctaaa gaatgccaac attgcggcag ctcgcgtcct tcccgggtgc caggacacag gcacggcgat tgttgccggc tacaagggcg aacaggtgtt cacaaacggc gatgaatgcg aggcgttgag tcgtggcgtc tacaagattt acaccacaac aaatctacgt tacagtcaaa atgttcctct caccatgttt gatgaaaaaa acacgggctc caatctgccg gcacagattg acttgtatgc gacgaaggga caagagtaca acttcatgtt tgtggcaaaa ggaggcggct cggcgaacaa ggcgtatctt ttccaggaga cgaagtcggt tcttaaccca aagtccctcc gtaagttttt agaggaaaaa attggcgcta ttggtaccgc ggcctgccca ccataccata tggctgtcgt cgtgggtggc acaagcgccg aaatgacact caagacagtg aagtacgcat cttgcaaata ctacgacaat ctacccacaa aaccggatga gtccaagggc tacgcctacc gcgacactga aatggagaat gtcgttatgg acatctgcta caacatgggc atgggggccc agtttggggg gaagtacttt gcccatgatg cccgcgtcat tcgcctgccc cgtcacggcg ccagttgtcc gataggcatc ggtgtaagct gcagcgccga tcgtcaggca cttgcaaaaa taaacaagga cggtatttgg cttgagcagc tcgaaacaga tcccgcaaaa tacctcccag agatcaccga agaccagctc cttaagacac cacccgtgaa tattgaccta agtatgccta tggagaagat tcgcgctgaa ctttcaaagt atcctgtcaa gacacgacta agtttgagtg gcaccatcat tgttgcccga gacatggccc atgcacggat gcgggaaatg cttgaggcgg gaaagccgtt accagaatac attaagaacc accccgtgta ctacgcaggg ccggcaaaat gtcctgaagg catgccttct ggttcctttg gtccaacgac agcgggacgc atggacccat ttgtggatct ctttcaggca aacggcggtt cctttgttat gctcgcaaaa ggaaaccgca gtcgagcagt aacgcaggca tgtaagaaac acggaggatt ctatcttggc agtatcggtg gccctgccgc actgcttgca aaagactcca ttcgcaaggt agaggtgctt gatatggcgg agttgggaat ggaggcggtg tggaagattg aagtggagaa ttttcccgcc ttcattgttt tggacgacaa gggaaatgac ttcttccaac agctcaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat