SSGCID: Target Page

TrcrB.18331.a

Fumarate hydratase, putative (class I, cytosolic)

CENTER ID:	TrcrB.18331.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18331.a.B1.GE42092	full length		1	565

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18331.a.B1.PW38458	full length		1	565

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tc00.1047053510329.270
UniProt:	Q4E3I6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLCENCNLA NECRGIQDIE DSKASASEFH YEPIFQPSDP HHDETEYYML EGDYIKTVEI LGKTILQVKP EALTALAQQA FADVHHFFRK DHLFGWRRIL EDPEASNNDR FVAHTLLQNA CVAAGRVLPA CQDTGTAIVL GKRGELCWTG GEDEKHLSKG IWDCYMNRYL RYSQTAALDM FKEVNTGNNL PAQFDLLATA GNEYHFLFIA KGGGSANKAF LYQETKALLN PKSLREFIEQ KLKTLGTAAC PPYHIALVIG GTSAEMTMKT VKLASCRYYD SLPTTGNKNG RAFRDLEWEA IVMDIACKSG IGAQFGGKYF AHQARVIRLP RHGASCPVGL GVSCSADRQI LAKITPKGVF VERLVRDPAQ YLPDLPPSSL STNVVNVNLN QPMSEIRKLL SQYPVTTRLS LTGTLIVARD IAHAKIMERL NNNEELPQYM KDHPVYYAGP AKTPKGMASG SFGPTTAGRM DSYLCPFMAA GGSFVTLAKG NRSKAVTEAC KKYGGFYLGS IGGSAAILAQ NNIKNVEVVE FPELGMEAVW RIDVVDFPAF IVVDDKGNDF FSKLL

NT Sequence

atgagtctgt gcgagaactg caatttggcg aatgagtgcc gtggcatcca agacattgaa gattcaaagg cttcggcatc agagttccac tacgagccaa tcttccagcc ttctgatccg caccacgacg agacagagta ctacatgctt gaaggcgatt atattaagac ggtggaaatt cttgggaaaa cgatcttgca agtaaagccg gaagcactca ctgctttggc gcaacaagcc tttgccgacg tacatcactt cttccgtaag gatcaccttt ttggatggcg acgcattctt gaggatcctg aggcctcaaa taatgatcgc tttgtcgccc acacgctcct gcagaatgcc tgtgttgctg ctggtcgtgt ccttccggca tgtcaggata cggggacggc gattgtcctc ggcaaacgtg gcgaactctg ttggacggga ggagaggacg aaaagcacct ttccaagggt atttgggact gttacatgaa ccggtatctg cgttacagcc agacggcggc gcttgacatg tttaaagagg tgaacactgg aaataacctg ccggcacagt ttgacctgtt ggcgacagct ggaaatgagt atcactttct ctttattgca aagggtgggg gcagtgcaaa taaggccttc ctttaccaag agaccaaggc actgctgaat cccaagtctc tcagggaatt tattgagcag aagcttaaga ccctcggcac ggcggcgtgt cccccgtatc acattgctct tgtcattggc ggcacaagtg cggaaatgac gatgaagacg gtgaagctcg ccagttgtcg ctattacgac tctttgccca caactggcaa caagaatggt cgtgctttcc gggatcttga gtgggaagca atcgtgatgg atatcgcctg taaatctggt attggagcac aatttggggg aaaatacttt gctcatcaag ctcgtgtcat tcgtctacct cgccatggtg cgtcgtgccc cgttggcttg ggtgtctcct gcagcgcgga ccgtcagatt cttgcaaaga tcacaccaaa gggtgtattc gtggagcggc ttgtgcggga ccccgcacaa tacctgccag acttaccacc ctcctctctg agcacgaacg tggtgaatgt caatctgaac cagccgatga gcgagatccg caagctgctc agtcagtacc ctgttaccac ccgcctcagt cttactggga ctctcattgt ggcgcgcgac attgcccatg cgaagatcat ggagcgtttg aataacaacg aagaacttcc tcagtacatg aaggatcacc cggtgtacta cgccggccca gcgaagactc caaagggaat ggcctccggc tcctttgggc ccacaacagc aggtcgtatg gactcttact tatgcccctt catggccgcc ggtggaagct ttgtcacgct tgcaaaaggg aaccgctcaa aggcggtgac agaagcgtgc aagaagtacg gtggcttcta tcttggaagc attggaggat ccgcggctat cttggcgcag aacaatatca agaacgttga ggttgtggag ttcccagagc ttgggatgga agcggtgtgg cgcatcgatg tggtggactt ccctgccttt attgtcgttg atgacaaggg caatgacttt ttttccaaac tcctttaa

Details for TrcrB.18331.a.B1.GE42092

HARVESTED ON:	12/5/2017
SEQUENCED ON:	12/7/2017
EXPECTED MW:	63kDa
OBSERVED MW:	63kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS LCENCNLANE CRGIQDIEDS KASASEFHYE PIFQPSDPHH DETEYYMLEG DYIKTVEILG KTILQVKPEA LTALAQQAFA DVHHFFRKDH LFGWRRILED PEASNNDRFV AHTLLQNACV AAGRVLPACQ DTGTAIVLGK RGELCWTGGE DEKHLSKGIW DCYMNRYLRY SQTAALDMFK EVNTGNNLPA QFDLLATAGN EYHFLFIAKG GGSANKAFLY QETKALLNPK SLREFIEQKL KTLGTAACPP YHIALVIGGT SAEMTMKTVK LASCRYYDSL PTTGNKNGRA FRDLEWEAIV MDIACKSGIG AQFGGKYFAH QARVIRLPRH GASCPVGLGV SCSADRQILA KITPKGVFVE RLVRDPAQYL PDLPPSSLST NVVNVNLNQP MSEIRKLLSQ YPVTTRLSLT GTLIVARDIA HAKIMERLNN NEELPQYMKD HPVYYAGPAK TPKGMASGSF GPTTAGRMDS YLCPFMAAGG SFVTLAKGNR SKAVTEACKK YGGFYLGSIG GSAAILAQNN IKNVEVVEFP ELGMEAVWRI DVVDFPAFIV VDDKGNDFFS KLL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc tgtgcgagaa ctgcaatttg gcgaatgagt gccgtggcat ccaagacatt gaagattcaa aggcttcggc atcagagttc cactacgagc caatcttcca gccttctgat ccgcaccacg acgagacaga gtactacatg cttgaaggcg attatattaa gacggtggaa attcttggga aaacgatctt gcaagtaaag ccggaagcac tcactgcttt ggcgcaacaa gcctttgccg acgtacatca cttcttccgt aaggatcacc tttttggatg gcgacgcatt cttgaggatc ctgaggcctc aaataatgat cgctttgtcg cccacacgct cctgcagaat gcctgtgttg ctgctggtcg tgtccttccg gcatgtcagg atacggggac ggcgattgtc ctcggcaaac gtggcgaact ctgttggacg ggaggagagg acgaaaagca cctttccaag ggtatttggg actgttacat gaaccggtat ctgcgttaca gccagacggc ggcgcttgac atgtttaaag aggtgaacac tggaaataac ctgccggcac agtttgacct gttggcgaca gctggaaatg agtatcactt tctctttatt gcaaagggtg ggggcagtgc aaataaggcc ttcctttacc aagagaccaa ggcactgctg aatcccaagt ctctcaggga atttattgag cagaagctta agaccctcgg cacggcggcg tgtcccccgt atcacattgc tcttgtcatt ggcggcacaa gtgcggaaat gacgatgaag acggtgaagc tcgccagttg tcgctattac gactctttgc ccacaactgg caacaagaat ggtcgtgctt tccgggatct tgagtgggaa gcaatcgtga tggatatcgc ctgtaaatct ggtattggag cacaatttgg gggaaaatac tttgctcatc aagctcgtgt cattcgtcta cctcgccatg gtgcgtcgtg ccccgttggc ttgggtgtct cctgcagcgc ggaccgtcag attcttgcaa agatcacacc aaagggtgta ttcgtggagc ggcttgtgcg ggaccccgca caatacctgc cagacttacc accctcctct ctgagcacga acgtggtgaa tgtcaatctg aaccagccga tgagcgagat ccgcaagctg ctcagtcagt accctgttac cacccgcctc agtcttactg ggactctcat tgtggcgcgc gacattgccc atgcgaagat catggagcgt ttgaataaca acgaagaact tcctcagtac atgaaggatc acccggtgta ctacgccggc ccagcgaaga ctccaaaggg aatggcctcc ggctcctttg ggcccacaac agcaggtcgt atggactctt acttatgccc cttcatggcc gccggtggaa gctttgtcac gcttgcaaaa gggaaccgct caaaggcggt gacagaagcg tgcaagaagt acggtggctt ctatcttgga agcattggag gatccgcggc tatcttggcg cagaacaata tcaagaacgt tgaggttgtg gagttcccag agcttgggat ggaagcggtg tggcgcatcg atgtggtgga cttccctgcc tttattgtcg ttgatgacaa gggcaatgac tttttttcca aactcctttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for TrcrB.18331.a.B1.PW38458

PURIFICATION DATe:	4/23/2018
CONCENTRATION:	13.06mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTC TGTGCGAGAA CTGCAATTTG GCGAATGAGT GCCGTGGCAT CCAAGACATT GAAGATTCAA AGGCTTCGGC ATCAGAGTTC CACTACGAGC CAATCTTCCA GCCTTCTGAT CCGCACCACG ACGAGACAGA GTACTACATG CTTGAAGGCG ATTATATTAA GACGGTGGAA ATTCTTGGGA AAACGATCTT GCAAGTAAAG CCGGAAGCAC TCACTGCTTT GGCGCAACAA GCCTTTGCCG ACGTACATCA CTTCTTCCGT AAGGATCACC TTTTTGGATG GCGACGCATT CTTGAGGATC CTGAGGCCTC AAATAATGAT CGCTTTGTCG CCCACACGCT CCTGCAGAAT GCCTGTGTTG CTGCTGGTCG TGTCCTTCCG GCATGTCAGG ATACGGGGAC GGCGATTGTC CTCGGCAAAC GTGGCGAACT CTGTTGGACG GGAGGAGAGG ACGAAAAGCA CCTTTCCAAG GGTATTTGGG ACTGTTACAT GAACCGGTAT CTGCGTTACA GCCAGACGGC GGCGCTTGAC ATGTTTAAAG AGGTGAACAC TGGAAATAAC CTGCCGGCAC AGTTTGACCT GTTGGCGACA GCTGGAAATG AGTATCACTT TCTCTTTATT GCAAAGGGTG GGGGCAGTGC AAATAAGGCC TTCCTTTACC AAGAGACCAA GGCACTGCTG AATCCCAAGT CTCTCAGGGA ATTTATTGAG CAGAAGCTTA AGACCCTCGG CACGGCGGCG TGTCCCCCGT ATCACATTGC TCTTGTCATT GGCGGCACAA GTGCGGAAAT GACGATGAAG ACGGTGAAGC TCGCCAGTTG TCGCTATTAC GACTCTTTGC CCACAACTGG CAACAAGAAT GGTCGTGCTT TCCGGGATCT TGAGTGGGAA GCAATCGTGA TGGATATCGC CTGTAAATCT GGTATTGGAG CACAATTTGG GGGAAAATAC TTTGCTCATC AAGCTCGTGT CATTCGTCTA CCTCGCCATG GTGCGTCGTG CCCCGTTGGC TTGGGTGTCT CCTGCAGCGC GGACCGTCAG ATTCTTGCAA AGATCACACC AAAGGGTGTA TTCGTGGAGC GGCTTGTGCG GGACCCCGCA CAATACCTGC CAGACTTACC ACCCTCCTCT CTGAGCACGA ACGTGGTGAA TGTCAATCTG AACCAGCCGA TGAGCGAGAT CCGCAAGCTG CTCAGTCAGT ACCCTGTTAC CACCCGCCTC AGTCTTACTG GGACTCTCAT TGTGGCGCGC GACATTGCCC ATGCGAAGAT CATGGAGCGT TTGAATAACA ACGAAGAACT TCCTCAGTAC ATGAAGGATC ACCCGGTGTA CTACGCCGGC CCAGCGAAGA CTCCAAAGGG AATGGCCTCC GGCTCCTTTG GGCCCACAAC AGCAGGTCGT ATGGACTCTT ACTTATGCCC CTTCATGGCC GCCGGTGGAA GCTTTGTCAC GCTTGCAAAA GGGAACCGCT CAAAGGCGGT GACAGAAGCG TGCAAGAAGT ACGGTGGCTT CTATCTTGGA AGCATTGGAG GATCCGCGGC TATCTTGGCG CAGAACAATA TCAAGAACGT TGAGGTTGTG GAGTTCCCAG AGCTTGGGAT GGAAGCGGTG TGGCGCATCG ATGTGGTGGA CTTCCCTGCC TTTATTGTCG TTGATGACAA GGGCAATGAC TTTTTTTCCA AACTCCTTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT