TrcrB.18236.a

KREL2

CENTER ID: TrcrB.18236.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18236.a.B1.GE40798 full length 1 416
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TcCLB.506363.110
RefSeq: XP_811386.1
UniProt: Q4DAH8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRRHFQLFL RRTPLWLADD GSSLFERYTE IENSNERRMS ALKACGMFDD EWIATEKVHG ANFGIYSIEG EKTIRYAKRS GIMHPTEHFF GYHMLVPQLQ QYITVTREML CDKLQKKLHT VLINGELFGG KYDHPSLQKT RKTVMVAGKP RTISAVQTDS FPQYSPDLHF YAFDIKYKET NEAEYVTLTF DDATELFKRV PGLLYARAII RGPMSKVAAF DVEHFVTTIP PLVGMGNYPL KGNWAEGLVV KHAKRGTPGF DPKGLTILKF KCTAFQEIST DRRQGPRVDE MESVRRDSIS RSGIQLPALE SIIHDPVQLE ASKFLLDHIC ENRLNAVLSK IGTDPFEKQE MTPDDLATLL AKDALKDFLK EAEPAIVNTP ILTRRDMARY VLFESRQLVC SRWKAILQRQ TADVVE
NT Sequence
atgctgcggc gccactttca gctgtttctt cgtaggacgc ccctgtggct tgctgacgat ggttcttctc tttttgagcg ttacacagaa attgagaact ctaacgaacg acggatgagt gcattgaagg catgcggaat gtttgacgat gagtggattg ccacagagaa ggttcatggc gccaactttg ggatttactc catcgaaggt gagaaaacca tacgttacgc gaaaagaagc ggtattatgc atcccactga acatttcttt ggctatcata tgttggtacc gcagttgcag cagtacatca ccgtcacccg tgagatgctc tgtgacaagt tgcagaagaa gttgcacact gttcttataa acggcgagtt gtttgggggg aagtacgacc acccaagtct tcaaaagacg cgaaaaacgg ttatggtggc aggtaaaccc cggacaatta gtgccgtgca gacggattcg tttcctcagt acagtcctga tcttcatttt tatgcgtttg acatcaaata taaggagact aacgaggctg aatacgttac tctcacattt gatgatgcaa ctgaattgtt taagagggtt cctggattac tgtatgcgag ggccatcatt cggggcccaa tgtcgaaggt ggcggccttt gatgtggagc actttgtcac caccattccg ccgcttgtcg ggatgggcaa ctacccatta aagggtaatt gggcagaggg acttgtagtg aagcatgcaa agcgaggcac gcctggcttt gaccccaagg gattgaccat tttgaagttt aagtgcaccg cctttcagga gatctcgaca gaccggcgtc aagggcctcg cgttgacgaa atggagagtg tgcgacggga ttcaattagc cgctcgggta ttcagctccc tgctctggaa agcatcatac atgatccagt gcagctggaa gcatcgaagt ttcttctgga tcacatctgt gagaatcgcc tgaacgccgt cctgtcaaag attggaacgg acccctttga gaagcaggag atgacgccgg atgatctggc gacactgttg gccaaggatg ccttgaagga ttttctcaag gaggcggaac ctgccatagt gaacacgccc attctcactc gcagggacat ggcaagatac gttctatttg aatcgaggca gctggtctgt tcacgttgga aggcgatttt acaacgacag acggccgatg ttgttgagtg a
Details for TrcrB.18236.a.B1.GE40798
HARVESTED ON: 1/31/2017
SEQUENCED ON: 11/22/2016
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML RRHFQLFLRR TPLWLADDGS SLFERYTEIE NSNERRMSAL KACGMFDDEW IATEKVHGAN FGIYSIEGEK TIRYAKRSGI MPPTEHFFGY HMLVPQLQQY ITVTREMLCD KLQKKLHTVL INGELFGGKY DHPSLQKTRK TVMVAGKPRT ISAVQTDSFP QYSPDLHFYA FDIKYKEINE AEYVTLTFDD ATELFKRVPG LLYARAIIRG PMSKVAAFDV EHFVTTIPPL VGMGNYPLKG NWAEGLVVKH AKRGTPGFDP KGLTILKFKC TAFQEISTDR RQGPRVDEME SVRRDSISRS GIQLPALESI IHDPVQLEAS KFLLDHICEN RLNAVLSKIG TDPFEKQEMT PDDLATLLAK DALKDFLKEA EPAIVNTPIL TRRDMARYVL FESRQLVCSR WKAILQR
Validated NT Sequence
gtcgttgtaa aatcgccttc caacgtgaac agaccagctg cctcgattca aatagaacgt atcttgccat gtccctgcga gtgagaatgg gcgtgttcac tatggcaggt tccgcctcct tgagaaaatc cttcaaggca tccttggcca acagtgtcgc cagatcatcc ggcgtcatct cctgcttctc aaaggggtcc gttccaatct ttgacaggac ggcgttcagg cgattctcac agatgtgatc cagaagaaac ttcgatgctt ccagctgcac tggatcatgt atgatgcttt ccagagcagg gagctgaata cccgagcggc taattgaatc ccgtcgcaca ctctccattt cgtcaacgcg aggcccttga cgccggtctg tcgagatctc ctgaaaggcg gtgcacttaa acttcaaaat ggtcaatccc ttggggtcaa agccaggcgt gcctcgcttt gcatgcttca ctacaagtcc ctctgcccaa ttacccttta atgggtagtt gcccatcccg acaagcggcg gaatggtggt gacaaagtgc tccacatcaa aggccgccac cttcgacatt gggccccgaa tgatggccct cgcatacagt aatccaggaa ccctcttaaa caattcagtt gcatcgtcaa atgtgagagt aacgtattca gcctcgttaa tctccttata tttgatgtca aatgcataaa aatgaagatc aggactgtac tgaggaaacg aatccgtctg cacggcacta attgtccggg gtttacctgc caccataacc gtttttcgcg tcttttgaag acttgggtgg tcgtacttcc ccccaaacaa ctcgccgttt ataagaacgg tgtgcaactt cttctgcaac ttgtcacaga gcatctcacg ggtgacggtg atgtactgct gcaactgcgg taccaacata tgatagccaa agaaatgctc agtgggaggc ataataccgc ttcttttcgc gtaacgtatg gttttctcac cttcgatgga gtaaatccca aagtttgcgc catgaacctt ctctgtggca atccactcat cgtcaaacat tccgcatgcc ttcagtgcac tcatccgtcg ttcgttagag ttctcaattt ctgtgtaacg ctcaaaaaga gaagaaccat cgtcagcaag ccacaggggc gtcctacgaa gaaacagctg aaagtggcgc cgcagcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHML RRHFQLFLRR TPLWLADDGS SLFERYTEIE NSNERRMSAL KACGMFDDEW IATEKVHGAN FGIYSIEGEK TIRYAKRSGI MHPTEHFFGY HMLVPQLQQY ITVTREMLCD KLQKKLHTVL INGELFGGKY DHPSLQKTRK TVMVAGKPRT ISAVQTDSFP QYSPDLHFYA FDIKYKETNE AEYVTLTFDD ATELFKRVPG LLYARAIIRG PMSKVAAFDV EHFVTTIPPL VGMGNYPLKG NWAEGLVVKH AKRGTPGFDP KGLTILKFKC TAFQEISTDR RQGPRVDEME SVRRDSISRS GIQLPALESI IHDPVQLEAS KFLLDHICEN RLNAVLSKIG TDPFEKQEMT PDDLATLLAK DALKDFLKEA EPAIVNTPIL TRRDMARYVL FESRQLVCSR WKAILQRQTA DVVE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc ggcgccactt tcagctgttt cttcgtagga cgcccctgtg gcttgctgac gatggttctt ctctttttga gcgttacaca gaaattgaga actctaacga acgacggatg agtgcattga aggcatgcgg aatgtttgac gatgagtgga ttgccacaga gaaggttcat ggcgccaact ttgggattta ctccatcgaa ggtgagaaaa ccatacgtta cgcgaaaaga agcggtatta tgcatcccac tgaacatttc tttggctatc atatgttggt accgcagttg cagcagtaca tcaccgtcac ccgtgagatg ctctgtgaca agttgcagaa gaagttgcac actgttctta taaacggcga gttgtttggg gggaagtacg accacccaag tcttcaaaag acgcgaaaaa cggttatggt ggcaggtaaa ccccggacaa ttagtgccgt gcagacggat tcgtttcctc agtacagtcc tgatcttcat ttttatgcgt ttgacatcaa atataaggag actaacgagg ctgaatacgt tactctcaca tttgatgatg caactgaatt gtttaagagg gttcctggat tactgtatgc gagggccatc attcggggcc caatgtcgaa ggtggcggcc tttgatgtgg agcactttgt caccaccatt ccgccgcttg tcgggatggg caactaccca ttaaagggta attgggcaga gggacttgta gtgaagcatg caaagcgagg cacgcctggc tttgacccca agggattgac cattttgaag tttaagtgca ccgcctttca ggagatctcg acagaccggc gtcaagggcc tcgcgttgac gaaatggaga gtgtgcgacg ggattcaatt agccgctcgg gtattcagct ccctgctctg gaaagcatca tacatgatcc agtgcagctg gaagcatcga agtttcttct ggatcacatc tgtgagaatc gcctgaacgc cgtcctgtca aagattggaa cggacccctt tgagaagcag gagatgacgc cggatgatct ggcgacactg ttggccaagg atgccttgaa ggattttctc aaggaggcgg aacctgccat agtgaacacg cccattctca ctcgcaggga catggcaaga tacgttctat ttgaatcgag gcagctggtc tgttcacgtt ggaaggcgat tttacaacga cagacggccg atgttgttga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat