SSGCID: Target Page

TrcrB.18222.a

glutaminyl-tRNA synthetase, putative

CENTER ID:	TrcrB.18222.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.18222.a.B1.GE42637	full length		1	571

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.508277.160
UniProt:	Q4E0Y0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTAPSTTAAS TAVPVLEVKK DLRLLQTGRP VAGYSNTPEL LEKHHALTGG KPYFRFPPEP NGFLHIGHAK SMNLNFGCAV AHGGKCYLRY DDTNPETEEQ VYIDAIQEMV RWMGWKPDWI TFSSDYFQQL YEFAIQLIKA GKAYVDHSTA DEIKKQRESR EESPWRNRSI EENLLHFEYM RQGRYAEGEA TLRVKIDMKS DNPNMRDFIA YRVKFSAHPH AKDKWCIYPS YDYTHCLVDS LEDIDYSLCT LEFETRRESY FWLLEQLNLW RPFVWEFSRL NVTGSLLSKR KINVLVKKGI VRGFDDPRLL TLAGMRRRGY TPEAINRFCD HVGITRSMNV IQSSMLEHIL RDDLDERTER RLMIVDPVKV VIDNWDGEME VMCLNHPRKP DLQSRKVMFT KTFYIDRSDF RTEDNNSKFY GLAPGPRPVG LKYSGNIICR GYECDEAGRP SLIHVEVDFG RTTKPKTNIT WVSEKYAIPV EFRLYGDLLK DDRAAIDPEF LKYINSDSEQ VQQGYAEPEI QNAKVFESVQ AERFGYFVVD PDTRPGHLVM NRVLTLKEDK EKGAMAPCPQ K

NT Sequence

atgacagccc catccacgac tgctgcctcc acggctgtgc ccgtcttgga ggtaaaaaag gatcttcgcc tgctgcagac agggcggccg gtggcggggt actccaacac accggagctg ctggaaaagc atcatgcctt gacgggaggg aagccctact tccgcttccc gccggagccc aacggctttc tccacattgg ccatgcgaag agtatgaacc tgaactttgg ctgtgccgtg gcgcatggtg gcaagtgtta tctgcgctac gatgacacga accccgagac ggaggagcag gtgtacatcg acgccatcca ggaaatggtg cgctggatgg ggtggaagcc ggactggatc acattttctt cggattattt ccagcaactt tacgaatttg cgattcaact tattaaggct ggcaaggcgt atgtggacca ctccaccgcg gatgaaatta agaagcagcg cgagagtcgc gaggaaagcc cgtggcgcaa tcgctccatc gaagaaaatc ttctccattt tgagtacatg cgacaggggc gatatgcaga aggggaagca acactacggg taaaaattga tatgaagagt gataacccaa atatgcgtga ttttattgcg taccgcgtca agttttctgc gcacccgcac gccaaggaca agtggtgtat ttaccctagc tacgactata cacattgcct cgtcgacagt ctggaagata tcgactacag cttgtgcaca ttggagtttg aaacacggcg cgaaagttat ttttggctgt tggaacaact gaacctctgg agaccctttg tttgggagtt tagtcgtctc aatgtgacag gctcgctgct gtcaaagcga aaaattaacg tgctggtgaa gaaaggtatt gtccgtggat tcgatgatcc tcgtctactg acgctggccg gtatgcgacg acgcggctac acaccggagg ccatcaaccg attctgtgat catgtcggca ttacacgctc catgaacgtc attcagagtt ctatgcttga acacattctt cgtgacgacc ttgacgaacg caccgagcgt cgtcttatga ttgtggaccc cgtcaaggtc gtcattgaca actgggacgg cgaaatggag gtgatgtgcc taaatcaccc acgaaagccc gatcttcagt cgcgaaaagt aatgtttacc aaaacctttt atattgaccg tagtgacttc cgtacagagg ataacaacag caaattctac ggcctggcgc ctggaccgcg tcctgtgggg ctgaagtaca gcgggaatat catctgccgc ggatacgagt gcgatgaggc tggacgacct tctctcattc atgttgaggt ggactttggg cggacgacaa aacccaagac gaacattact tgggtcagtg agaagtacgc aattcccgta gaattccggc tttacggaga tcttctgaag gacgatcgtg ccgccattga cccggagttc ctaaagtaca tcaattccga cagcgagcag gtccagcagg gttacgccga gccggaaata caaaacgcga aggtgtttga atccgtgcag gccgagcgtt ttgggtattt tgttgtggac ccagacacac gtcctggcca cctcgtcatg aaccgggtgc tgacgctcaa ggaggacaag gagaagggtg ccatggcccc ctgtcctcaa aagtag

Details for TrcrB.18222.a.B1.GE42637

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	67kDa
OBSERVED MW:	67kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	83
PERCENT COVERAGE:	52

Validated AA Sequence

Validated NT Sequence

cccttctcct tgtcctcctt gagcgtcagc acccggttca tgacgaggtg gccaggacgt gtgtctgggt ccacaacaaa atacccaaaa cgctcggcct gcacggattc aaacaccttc gcgttttgta tttccggctc ggcgtaaccc tgctggacct gctcgctgtc ggaattgatg tactttagga actccgggtc aatggcggca cgatcgtcct tcagaagatc tccgtaaagc cggaattcta cgggaattgc gtacttctca ctgacccaag taatgttcgt cttgggtttt gtcgtccgcc caaagtccac ctcaacatga atgagagaag gtcgtccagc ctcatcgcac tcgtatccgc ggcagatgat attcccgctg tacttcagcc ccacaggacg cggtccaggc gccaggccgt agaatttgct gttgttatcc tctgtacgga agtcactacg gtcaatataa aaggttttgg taaacattac ttttcgcgac tgaagatcgg gctttcgtgg gtgatttagg cacatcacct ccatttcgcc gtcccagttg tcaatgacga ccttgacggg gtccacaatc ataagacgac gctcggtgcg ttcgtcaagg tcgtcacgaa gaatgtgttc nnncatagaa ctctgaatga cgttcatgga gcgtgtaatg ccgacatgat cacagnatcn gttgatggcc tccggtgtnn nnccgcgtcg tcgcataccg gccagcgtca nnngacgnnn atcatngnat ccacggacaa tacctttctt caccagnnnn ttaatttnnn nnnnnnnnan nnngcnnnnn nntnacattg nnnnnnnnnn nnnnnnnnnn aannnnnnnn cnnnngnnnn nnnnnnnnnn nngccaaaaa nnnnnntnnn nnnnnnnttn nnnnnnnnnn tgtgcacaan nnntnnnnga tannntccag actgtcgacg nngcaatgtg tatagtcgta nnnagggtaa atacaccact tgtccttggc gtgcgggtgc gcagaaaact tgacgcggta cgcaataaaa tcacgcatat ttgggttatc actcttcata tcaattttta cccgtagtgt tgcttcccct tctgcatatc gcccctgtcg catgtactca aaatggagaa gattttcttc gatggagcga ttgcgccacg ggctttcctc gcgactctcg cgctgcttct taatttcatc cgcggtggag tggtccacat acgccttgcc agccttaata agttgaatcg caaattcgta aagttgctgg aaataatccg aagaaaatgt gatccagtcc ggcttccacc ccatccagcg caccatttcc tggatggcgt cgatgtacac ctgctcctcc gtctcggggt tcgtgtcatc gtagcgcaga taacacttgc caccatgcgc cacggcacag ccaaagttca ggttcatact cttcgcatgg ccaatgtgga gaaagccgtt gggctccggc gggaagcgga agtagggctt ccctcccgtc aaggcatgat gcttttccag cagctccggt gtgttggagt accccgccac cggccgccct gtctgcagca ggcgaagatc cttttttacc tccaagacgg gcacagccgt ggaggcagca gtcgtggatg gggctgtcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHMT APSTTAASTA VPVLEVKKDL RLLQTGRPVA GYSNTPELLE KHHALTGGKP YFRFPPEPNG FLHIGHAKSM NLNFGCAVAH GGKCYLRYDD TNPETEEQVY IDAIQEMVRW MGWKPDWITF SSDYFQQLYE FAIQLIKAGK AYVDHSTADE IKKQRESREE SPWRNRSIEE NLLHFEYMRQ GRYAEGEATL RVKIDMKSDN PNMRDFIAYR VKFSAHPHAK DKWCIYPSYD YTHCLVDSLE DIDYSLCTLE FETRRESYFW LLEQLNLWRP FVWEFSRLNV TGSLLSKRKI NVLVKKGIVR GFDDPRLLTL AGMRRRGYTP EAINRFCDHV GITRSMNVIQ SSMLEHILRD DLDERTERRL MIVDPVKVVI DNWDGEMEVM CLNHPRKPDL QSRKVMFTKT FYIDRSDFRT EDNNSKFYGL APGPRPVGLK YSGNIICRGY ECDEAGRPSL IHVEVDFGRT TKPKTNITWV SEKYAIPVEF RLYGDLLKDD RAAIDPEFLK YINSDSEQVQ QGYAEPEIQN AKVFESVQAE RFGYFVVDPD TRPGHLVMNR VLTLKEDKEK GAMAPCPQK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag ccccatccac gactgctgcc tccacggctg tgcccgtctt ggaggtaaaa aaggatcttc gcctgctgca gacagggcgg ccggtggcgg ggtactccaa cacaccggag ctgctggaaa agcatcatgc cttgacggga gggaagccct acttccgctt cccgccggag cccaacggct ttctccacat tggccatgcg aagagtatga acctgaactt tggctgtgcc gtggcgcatg gtggcaagtg ttatctgcgc tacgatgaca cgaaccccga gacggaggag caggtgtaca tcgacgccat ccaggaaatg gtgcgctgga tggggtggaa gccggactgg atcacatttt cttcggatta tttccagcaa ctttacgaat ttgcgattca acttattaag gctggcaagg cgtatgtgga ccactccacc gcggatgaaa ttaagaagca gcgcgagagt cgcgaggaaa gcccgtggcg caatcgctcc atcgaagaaa atcttctcca ttttgagtac atgcgacagg ggcgatatgc agaaggggaa gcaacactac gggtaaaaat tgatatgaag agtgataacc caaatatgcg tgattttatt gcgtaccgcg tcaagttttc tgcgcacccg cacgccaagg acaagtggtg tatttaccct agctacgact atacacattg cctcgtcgac agtctggaag atatcgacta cagcttgtgc acattggagt ttgaaacacg gcgcgaaagt tatttttggc tgttggaaca actgaacctc tggagaccct ttgtttggga gtttagtcgt ctcaatgtga caggctcgct gctgtcaaag cgaaaaatta acgtgctggt gaagaaaggt attgtccgtg gattcgatga tcctcgtcta ctgacgctgg ccggtatgcg acgacgcggc tacacaccgg aggccatcaa ccgattctgt gatcatgtcg gcattacacg ctccatgaac gtcattcaga gttctatgct tgaacacatt cttcgtgacg accttgacga acgcaccgag cgtcgtctta tgattgtgga ccccgtcaag gtcgtcattg acaactggga cggcgaaatg gaggtgatgt gcctaaatca cccacgaaag cccgatcttc agtcgcgaaa agtaatgttt accaaaacct tttatattga ccgtagtgac ttccgtacag aggataacaa cagcaaattc tacggcctgg cgcctggacc gcgtcctgtg gggctgaagt acagcgggaa tatcatctgc cgcggatacg agtgcgatga ggctggacga ccttctctca ttcatgttga ggtggacttt gggcggacga caaaacccaa gacgaacatt acttgggtca gtgagaagta cgcaattccc gtagaattcc ggctttacgg agatcttctg aaggacgatc gtgccgccat tgacccggag ttcctaaagt acatcaattc cgacagcgag caggtccagc agggttacgc cgagccggaa atacaaaacg cgaaggtgtt tgaatccgtg caggccgagc gttttgggta ttttgttgtg gacccagaca cacgtcctgg ccacctcgtc atgaaccggg tgctgacgct caaggaggac aaggagaagg gtgccatggc cccctgtcct caaaagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t