TrcrB.18219.b

Glycylpeptide N-tetradecanoyltransferase (EC 2.3.1.97) (N-myristoyl transferase) (NMT)

CENTER ID: TrcrB.18219.b
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.18219.b.B1.GE42091 full length 1 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tc00.1047053506525.80
UniProt: Q4DK26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEEGSGLHQ FWNTQPVPQS SIDAADTVGP LEAAGTVDDV PTDPVAIAST LEWWSPDMDN KDDVRAIYEL LRDNYVEDVE SMFRFNYSED FLRWALTPPG YHSSWHVAVR RKRDQMLMGF VSGIPVTMRM GAPKKVLQKN KTTEEKEQGP EEKNNDHKSQ AEELYLEPRK ICEINFLCVH KLLRAKRLAP ILIKEVTRRV HLMNIWQAVY TAGRLLPTPF ATADYYHRSL NPEKLVAVGF SAIPQQYQKF QNPLSMIKRF YELPAKPKTR GLRPMEPKDA PQVANLLRKK LATCDVAPVF TDEEVAHYTL PREGVLMSYV VEREVSGGGG GGRVDSSRGK QNDAGTQKQI TDFFSFFSLP SSIIGSSKHS VLNAAYVFYS ANTTISLVHL MSDLLIVAHQ QGFDVCNVVN IMDNGDYLSE LKFGRGDGNL HYYFYNWSYP IVQPSDVGLF ML
NT Sequence
atggcagaag agggttcggg tttacatcag ttctggaaca cgcagcctgt cccgcagtcg agtattgatg cggccgatac ggtggggccg ctggaagcag cggggaccgt cgatgatgtc cccaccgatc cggtggcaat cgcctcaaca ctggagtggt ggtccccaga catggataac aaagatgatg tacgtgccat atacgagctt ctgcgcgata attacgttga ggatgttgag agcatgttcc gatttaacta ttcggaggat tttttgcgct gggcgcttac cccgccgggg tatcatagct catggcatgt ggcggtccgg cgtaagcgtg accaaatgtt gatggggttt gtctctggca ttccagtcac aatgcgtatg ggcgccccaa agaaggtact ccagaaaaac aagaccactg aagaaaaaga gcaagggccg gaggagaaga ataatgatca taagagtcag gcggaggagc tttacctcga acctcgtaag atctgcgaga tcaattttct ttgtgtgcat aagttattgc gggcgaagcg gttggcaccg attctcatta aggaggtaac acgtcgggtg catctgatga acatttggca ggctgtctat actgctggca ggttactgcc gacaccgttt gcgactgccg actattacca tcgcagcttg aacccggaaa agcttgtggc ggttggattt agcgcgatac cgcagcaata ccaaaagttt caaaacccat tgtccatgat aaaacgcttt tatgaacttc ccgccaaacc gaaaacacgt ggtcttcgac caatggaacc aaaggacgcg ccacaagtag cgaacttgct gcgaaagaaa cttgccacat gtgacgttgc ccccgtattt accgatgagg aggtggctca ttacactctg ccccgtgagg gtgttttgat gagctacgtg gtagagcgtg aagtcagtgg tggtggtggt ggtggtaggg ttgatagtag ccgcggcaaa cagaatgatg ctgggacaca gaagcaaatt acagatttct tttcattctt ctcacttcca tcttccatta ttggaagtag caaacattcc gttttaaatg cagcctatgt gttttacagc gccaacacaa caatttctct cgtgcatcta atgagcgatc ttttgattgt agcgcatcaa caaggctttg atgtttgtaa cgttgtgaac attatggaca atggtgatta tctaagcgaa ctcaagtttg gtcgaggaga tggtaatctg cactactact tctacaactg gtcgtaccct attgtgcagc caagtgacgt gggattgttc atgctatag
Details for TrcrB.18219.b.B1.GE42091
HARVESTED ON: data unavailable
SEQUENCED ON: 12/7/2017
EXPECTED MW: 52kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA EEGSGLHQFW NTQPVPQSSI DAADTVGPLE AAGTVDDVPT DPVAIASTLE WWSPDMDNKD DVRAIYELLR DNYVEDVESM FRFNYSEDFL RWALTPPGYH SSWHVAVRRK RDQMLMGFVS GIPVTMRMGA PKKVLQKNKT TEEKEQGPEE KNNDHKSQAE ELYLEPRKIC EINFLCVHKL LRAKRLAPIL IKEVTRRVHL MNIWQAVYTA GRLLPTPFAT ADYYHRSLNP EKLVAVGFSA IPQQYQKFQN PLSMIKRFYE LPAKPKTRGL RPMEPKDAPQ VANLLRKKLA TCDVAPVFTD EEVAHYTLPR EGVLMSYVVE REVSGGGGGG RVDSSRGKQN DAGTQKQITD FFSFFSLPSS IIGSSKHSVL NAAYVFYSAN TTISLVHLMS DLLIVAHQQG FDVCNVVNIM DNGDYLSELK FGRGDGNLHY YFYNWSYPIV QP
Validated NT Sequence
ttggctgcac aatagggtac gaccagttgt agaagtagta gtgcagatta ccatctcctc gaccaaactt gagttcgctt agataatcac cattgtccat aatgttcaca acgttacaaa catcaaagcc ttgttgatgc gctacaatca aaagatcgct cattagatgc acgagagaaa ttgttgtgtt ggcgctgtaa aacacatagg ctgcatttaa aacggaatgt ttgctacttc caataatgga agatggaagt gagaagaatg aaaagaaatc tgtaatttgc ttctgtgtcc cagcatcatt ctgtttgccg cggctactat caaccctacc accaccacca ccaccactga cttcacgctc taccacgtag ctcatcaaaa caccctcacg gggcagagtg taatgagcca cctcctcatc ggtaaatacg ggggcaacgt cacatgtggc aagtttcttt cgcagcaagt tcgctacttg tggcgcgtcc tttggttcca ttggtcgaag accacgtgtt ttcggtttgg cgggaagttc ataaaagcgt tttatcatgg acaatgggtt ttgaaacttt tggtattgct gcggtatcgc gctaaatcca accgccacaa gcttttccgg gttcaagctg cgatggtaat agtcggcagt cgcaaacggt gtcggcagta acctgccagc agtatagaca gcctgccaaa tgttcatcag atgcacccga cgtgttacct ccttaatgag aatcggtgcc aaccgcttcg cccgcaataa cttatgcaca caaagaaaat tgatctcgca gatcttacga ggttcgaggt aaagctcctc cgcctgactc ttatgatcat tattcttctc ctccggccct tgctcttttt cttcagtggt cttgtttttc tggagtacct tctttggggc gcccatacgc attgtgactg gaatgccaga gacaaacccc atcaacattt ggtcacgctt acgccggacc gccacatgcc atgagctatg ataccccggc ggggtaagcg cccagcgcaa aaaatcctcc gaatagttaa atcggaacat gctctcaaca tcctcaacgt aattatcgcg cagaagctcg tatatggcac gtacatcatc tttgttatcc atgtctgggg accaccactc cagtgttgag gcgattgcca ccggatcggt ggggacatca tcgacggtcc ccgctgcttc cagcggcccc accgtatcgg ccgcatcaat actcgactgc gggacaggct gcgtgttcca gaactgatgt aaacccgaac cctcttctgc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMA EEGSGLHQFW NTQPVPQSSI DAADTVGPLE AAGTVDDVPT DPVAIASTLE WWSPDMDNKD DVRAIYELLR DNYVEDVESM FRFNYSEDFL RWALTPPGYH SSWHVAVRRK RDQMLMGFVS GIPVTMRMGA PKKVLQKNKT TEEKEQGPEE KNNDHKSQAE ELYLEPRKIC EINFLCVHKL LRAKRLAPIL IKEVTRRVHL MNIWQAVYTA GRLLPTPFAT ADYYHRSLNP EKLVAVGFSA IPQQYQKFQN PLSMIKRFYE LPAKPKTRGL RPMEPKDAPQ VANLLRKKLA TCDVAPVFTD EEVAHYTLPR EGVLMSYVVE REVSGGGGGG RVDSSRGKQN DAGTQKQITD FFSFFSLPSS IIGSSKHSVL NAAYVFYSAN TTISLVHLMS DLLIVAHQQG FDVCNVVNIM DNGDYLSELK FGRGDGNLHY YFYNWSYPIV QPSDVGLFML
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag aagagggttc gggtttacat cagttctgga acacgcagcc tgtcccgcag tcgagtattg atgcggccga tacggtgggg ccgctggaag cagcggggac cgtcgatgat gtccccaccg atccggtggc aatcgcctca acactggagt ggtggtcccc agacatggat aacaaagatg atgtacgtgc catatacgag cttctgcgcg ataattacgt tgaggatgtt gagagcatgt tccgatttaa ctattcggag gattttttgc gctgggcgct taccccgccg gggtatcata gctcatggca tgtggcggtc cggcgtaagc gtgaccaaat gttgatgggg tttgtctctg gcattccagt cacaatgcgt atgggcgccc caaagaaggt actccagaaa aacaagacca ctgaagaaaa agagcaaggg ccggaggaga agaataatga tcataagagt caggcggagg agctttacct cgaacctcgt aagatctgcg agatcaattt tctttgtgtg cataagttat tgcgggcgaa gcggttggca ccgattctca ttaaggaggt aacacgtcgg gtgcatctga tgaacatttg gcaggctgtc tatactgctg gcaggttact gccgacaccg tttgcgactg ccgactatta ccatcgcagc ttgaacccgg aaaagcttgt ggcggttgga tttagcgcga taccgcagca ataccaaaag tttcaaaacc cattgtccat gataaaacgc ttttatgaac ttcccgccaa accgaaaaca cgtggtcttc gaccaatgga accaaaggac gcgccacaag tagcgaactt gctgcgaaag aaacttgcca catgtgacgt tgcccccgta tttaccgatg aggaggtggc tcattacact ctgccccgtg agggtgtttt gatgagctac gtggtagagc gtgaagtcag tggtggtggt ggtggtggta gggttgatag tagccgcggc aaacagaatg atgctgggac acagaagcaa attacagatt tcttttcatt cttctcactt ccatcttcca ttattggaag tagcaaacat tccgttttaa atgcagccta tgtgttttac agcgccaaca caacaatttc tctcgtgcat ctaatgagcg atcttttgat tgtagcgcat caacaaggct ttgatgtttg taacgttgtg aacattatgg acaatggtga ttatctaagc gaactcaagt ttggtcgagg agatggtaat ctgcactact acttctacaa ctggtcgtac cctattgtgc agccaagtga cgtgggattg ttcatgctat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat