SSGCID: Target Page

TrcrB.10201.a

methionyl-tRNA synthetase, putative

CENTER ID:	TrcrB.10201.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.10201.a.B1.GE42636	full length		1	765

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.509247.50
UniProt:	Q4D6H2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLKLFSEKA NTQALKALLC CHHVKRPVEL TLSGAYTSPV LHHPALKQPI FSANEMVRLI LHDKWRDDSA GGSGGKKTGC YDGHWASVSL EEEAWLEWEA TTFTCAVHPL YTQRRVTPEA AGAFEYLDNK IHENDCRGPC LAPVGEKGTS TKSPSFLVDC IIWCAVLPAL CEGGLLGEKE REKLPHLLKW FESFQSAREA HIAGAVESLA VQEFADFLRA PRVYKLSPKI SKVFFITTPI YYVNAAPHIG HVYSTLIVDV IGRYHTMKGE RVFAMSGTDE HGQKVAEAAR QKGMNPYDFT CQVSREFIDC FEQMGYHMDY FIRTTNESHK KVVHELWSKL EAKGDIYLGR YEGWYSVSDE SFLTAQNVTD GVDKDGKPCK VSLESGHVVT WLAEDNYMFR LSAFRERLLE WYNNNPGCIV PEFRRREVIR TVEKGLNDLS VSRTREAVQD WAIPVPGNPN HCIYVWLDAL SNYYTASRLR LGKSGEELEL VEDHRVLERF PADIHVIGKD ILKFHAIYWP AFLISAGLPL PKTIVAHGWW TKDRKKISKS LGNVFDPVEK AREFGFDALK YFLLRETGFS DDGDYSDRNM ISRLNGELAD TLGNLVMRCT SAKINVHRVW PTPDAYNAKD EALIQLIKDL PGVVDHFFLI PDVQKALAAV FDLLRAINSY VTENAPWRLV KTDPDRLRTV LYITMEGTRL ATLMLSPVLS QKAPVIFDML GVPEANRVGV ENFGFGVVPS GTSIGDAVEG KVLFPKISLD KAQSA

NT Sequence

atgagcctca aattgttttc ggagaaggcg aacacgcagg cgctgaaggc gttgctctgc tgccatcacg tcaagcggcc ggtggagctg acactctccg gtgcgtacac aagtccggta cttcatcatc cggcattaaa gcagcccatc ttttctgcga atgagatggt tcgtttgatt ctgcacgata aatggagaga cgattccgct ggtgggagcg gcggcaagaa gactggttgc tatgatggcc actgggcgag tgtttcactc gaagaagagg cctggctgga gtgggaggcg accacattca catgcgcggt gcacccattg tacactcagc gtcgggtgac gccagaggca gcgggagcgt ttgagtatct tgataataag attcatgaga atgactgtcg gggtccctgc ctggctcctg tgggggagaa ggggacgtcc acaaaatccc cttcttttct tgttgattgc atcatttggt gtgcggtatt gccggcgctc tgcgagggcg gtctgctagg ggaaaaggag cgcgagaagt tgccacatct cttgaaatgg tttgagtcgt ttcagtctgc gcgagaagcg catatagctg gcgccgtgga atctcttgca gtgcaggagt ttgctgattt tttgcgcgcc ccccgtgtgt ataaattatc gccgaaaata agtaaagtct tttttattac aacccctatt tattacgtga atgccgcccc tcatattggt catgtctaca gcactcttat tgtggatgtc attgggcggt accacacgat gaaaggggag cgtgtctttg cgatgagtgg aacggacgag catggacaga aggtggcaga ggctgccagg caaaaaggga tgaatccgta cgattttacg tgtcaagtct caagggagtt tattgattgt tttgagcaaa tgggttacca tatggattac tttattcgaa ctacgaacga gagtcacaaa aaggttgtcc atgagttatg gtccaaactt gaggcgaagg gtgatattta cttgggtcgc tatgaggggt ggtattctgt cagcgatgag tcctttttga cggcacagaa tgtgacagac ggcgtggata aagacggcaa gccatgcaag gttagcctgg agagcggtca cgtggtgaca tggttggcgg aggataacta catgttccgc cttagcgcgt ttcgtgagcg cctgctggaa tggtacaaca acaaccccgg gtgcattgtt cccgagttcc gccgccgtga ggtcatccgt acagtggaaa agggtttaaa cgatctgagc gtctcgcgta cgagggaggc tgtgcaggac tgggcaatcc cggtccctgg aaaccccaac cactgcattt acgtctggct tgatgcactc tccaattatt acaccgcctc acgtctccgt ctaggaaagt ctggggagga gctggaactt gtcgaggacc acagagtgtt ggaacgtttt cctgctgaca tccatgtcat tggaaaggat attttaaagt ttcacgctat ttattggccg gcctttttga tttcggcggg ccttccatta cccaaaacga ttgtcgcgca tgggtggtgg acaaaggatc gcaaaaaaat cagcaaatcc cttggaaacg tctttgatcc tgtagaaaag gcacgtgagt tcggttttga cgcgcttaag tattttttgt tacgagagac tggcttttcg gatgatggtg actacagcga caggaacatg atttcccgcc tcaacggtga gcttgccgac acccttggaa atctcgtgat gcgttgcacc tccgccaaga tcaacgttca tcgtgtgtgg ccaaccccgg acgcctacaa tgcaaaggat gaggctctta ttcaactcat aaaggacttg ccgggagttg tggatcactt cttcttaatt ccggatgtgc aaaaggctct ggcagccgtg tttgatttgc ttcgcgccat caactcctac gtcacagaga acgcgccgtg gaggcttgtc aagacggacc cggatcggct gcgcactgtt ctgtacatca ccatggaggg gacccgcctg gccacactca tgttgagccc cgtcctctca cagaaggcac ccgtcatttt tgacatgttg ggcgtaccgg aggcgaatcg tgttggcgtg gagaattttg ggtttggcgt tgtgccgtct gggacgtcca ttggcgacgc ggtggaaggc aaggtgttgt tcccaaagat ttcgctggat aaagcgcagt ccgcttag

Details for TrcrB.10201.a.B1.GE42636

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	87kDa
OBSERVED MW:	87kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS LKLFSEKANT QALKALLCCH HVKRPVELTL SGAYTSPVLH HPALKQPIFS ANEMVRLILH DKWRDDSAGG SGGKKTGCYD GHWASVSLEE EAWLEWEATT FTCAVHPLYT QRRVTPEAAG AFEYLDNKIH ENDCRGPCLA PVGEKGTSTK SPSFLVDCII WCAVLPALCE GGLLGEKERE KLPHLLKWFE SFQSAREAHI AGAVESLAVQ EFADFLRAPR VYKLSPKISK VFFITTPIYY VNAAPHIGHV YSTLIVDVIG RYHTMKGERV FAMSGTDEHG QKVAEAARQK GMNPYDFTCQ VSREFIDCFE QMGYHMDYFI RTTNESHKKV VHELWSKLEA KGDIYLGRYE GWYSVSDESF LTAQNVTDGV DKDGKPCKVS LESGHVVTWL AEDNYMFRLS AFRERLLEWY NNNPGCIVPE FRRREVIRTV EKGLNDLSVS RTREAVQDWA IPVPGNPNHC IYVWLDALSN YYTASRLRLG KSGEELELVE DHRVLERFPA DIHVIGKDIL KFHAIYWPAF LISAGLPLPK TIVAHGWWTK DRKKISKSLG NVFDPVEKAR EFGFDALKYF LLRETGFSDD GDYSDRNMIS RLNGELADTL GNLVMRCTSA KINVHRVWPT PDAYNAKDEA LIQLIKDLPG VVDHFFLIPD VQKALAAVFD LLRAINSYVT ENAPWRLVKT DPDRLRTVLY ITMEGTRLAT LMLSPVLSQK APVIFDMLGV PEANRVGVEN FGFGVVPSGT SIGDAVEGKV LFPKISLDKA QSA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc tcaaattgtt ttcggagaag gcgaacacgc aggcgctgaa ggcgttgctc tgctgccatc acgtcaagcg gccggtggag ctgacactct ccggtgcgta cacaagtccg gtacttcatc atccggcatt aaagcagccc atcttttctg cgaatgagat ggttcgtttg attctgcacg ataaatggag agacgattcc gctggtggga gcggcggcaa gaagactggt tgctatgatg gccactgggc gagtgtttca ctcgaagaag aggcctggct ggagtgggag gcgaccacat tcacatgcgc ggtgcaccca ttgtacactc agcgtcgggt gacgccagag gcagcgggag cgtttgagta tcttgataat aagattcatg agaatgactg tcggggtccc tgcctggctc ctgtggggga gaaggggacg tccacaaaat ccccttcttt tcttgttgat tgcatcattt ggtgtgcggt attgccggcg ctctgcgagg gcggtctgct aggggaaaag gagcgcgaga agttgccaca tctcttgaaa tggtttgagt cgtttcagtc tgcgcgagaa gcgcatatag ctggcgccgt ggaatctctt gcagtgcagg agtttgctga ttttttgcgc gccccccgtg tgtataaatt atcgccgaaa ataagtaaag tcttttttat tacaacccct atttattacg tgaatgccgc ccctcatatt ggtcatgtct acagcactct tattgtggat gtcattgggc ggtaccacac gatgaaaggg gagcgtgtct ttgcgatgag tggaacggac gagcatggac agaaggtggc agaggctgcc aggcaaaaag ggatgaatcc gtacgatttt acgtgtcaag tctcaaggga gtttattgat tgttttgagc aaatgggtta ccatatggat tactttattc gaactacgaa cgagagtcac aaaaaggttg tccatgagtt atggtccaaa cttgaggcga agggtgatat ttacttgggt cgctatgagg ggtggtattc tgtcagcgat gagtcctttt tgacggcaca gaatgtgaca gacggcgtgg ataaagacgg caagccatgc aaggttagcc tggagagcgg tcacgtggtg acatggttgg cggaggataa ctacatgttc cgccttagcg cgtttcgtga gcgcctgctg gaatggtaca acaacaaccc cgggtgcatt gttcccgagt tccgccgccg tgaggtcatc cgtacagtgg aaaagggttt aaacgatctg agcgtctcgc gtacgaggga ggctgtgcag gactgggcaa tcccggtccc tggaaacccc aaccactgca tttacgtctg gcttgatgca ctctccaatt attacaccgc ctcacgtctc cgtctaggaa agtctgggga ggagctggaa cttgtcgagg accacagagt gttggaacgt tttcctgctg acatccatgt cattggaaag gatattttaa agtttcacgc tatttattgg ccggcctttt tgatttcggc gggccttcca ttacccaaaa cgattgtcgc gcatgggtgg tggacaaagg atcgcaaaaa aatcagcaaa tcccttggaa acgtctttga tcctgtagaa aaggcacgtg agttcggttt tgacgcgctt aagtattttt tgttacgaga gactggcttt tcggatgatg gtgactacag cgacaggaac atgatttccc gcctcaacgg tgagcttgcc gacacccttg gaaatctcgt gatgcgttgc acctccgcca agatcaacgt tcatcgtgtg tggccaaccc cggacgccta caatgcaaag gatgaggctc ttattcaact cataaaggac ttgccgggag ttgtggatca cttcttctta attccggatg tgcaaaaggc tctggcagcc gtgtttgatt tgcttcgcgc catcaactcc tacgtcacag agaacgcgcc gtggaggctt gtcaagacgg acccggatcg gctgcgcact gttctgtaca tcaccatgga ggggacccgc ctggccacac tcatgttgag ccccgtcctc tcacagaagg cacccgtcat ttttgacatg ttgggcgtac cggaggcgaa tcgtgttggc gtggagaatt ttgggtttgg cgttgtgccg tctgggacgt ccattggcga cgcggtggaa ggcaaggtgt tgttcccaaa gatttcgctg gataaagcgc agtccgcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat