TrcrB.01480.b

Aurora kinase 2, putative (AUK2)

CENTER ID: TrcrB.01480.b
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.01480.b.B2.GE43003 PHoranyi kinase domain truncation 106 401
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.503685.10
UniProt: Q4CQY7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWQHHNLSSP TLGPREAKPT VQTSRRGCMR SDAWCSSLAG GGHDDPSESV PNSRVSPRAS SFAPPQRTAP SPTESTSQEL FELQNCNHHK QQQKQQLVIR QEDIRDDDFQ RLEVLGDGSY SVVVSARHLP TQQLVALKEV SRHRLRELKL EKQLQWEINV HRTLRHPNVV RMLSYYITPQ TVVLVLEMCP GGTLLQKLKT MPGGRFDERR ASRYVRQAAR ALTYLHGHGI AHRDLKLENI FLDAHGVARL GDFGWSKALV EPTVEQDLSS HHDNFGTAPC SAAKDVSPLC SGGRGRLTVC GTLDYLSPEM LIGMPHTFKT DVWSLGVMLA EMLTGAPPFY CVSQQDTLNA IREAAPHLGD DVQMSSLARE LVLAMLQKNP DARPTMAEVL QHPWTRQNGE KKKKKK
NT Sequence
atgtggcagc accacaactt gagttccccg actctggggc cgcgtgaagc gaagccgacg gtgcagacct cacggcgggg atgcatgcga agtgacgcgt ggtgctcttc tttggcaggt ggcgggcacg acgatccgtc ggagtcggtg ccaaactcac gggtgtcacc gcgcgcctct tccttcgcgc cgccgcaacg cacggcacca tcaccgactg aatcgacgtc gcaggagttg tttgaattgc aaaattgcaa ccaccacaag cagcagcaga agcagcaact tgtcattcga caggaagaca tacgtgacga cgacttccag cggctagagg tgttgggcga cggttcttac tccgtggtgg tttccgcccg ccacctaccc acccagcagc ttgttgcgct gaaggaagta tcgcgacacc gcctccgtga gctgaagttg gagaagcagt tgcaatggga gatcaacgtt caccgcacgc tgcgtcatcc caatgtggtt cgaatgctga gctactatat caccccgcag accgttgtgc ttgtgttgga gatgtgtcca ggcgggacgc tcctgcagaa actgaagaca atgcccggtg gaaggtttga tgagcggcgg gcatcacggt acgttcggca ggccgcgcgg gcgctaacgt acctgcacgg gcacggaatt gcccaccgtg acttgaaact ggagaacatt tttttagacg cgcacggtgt ggcacggctg ggagattttg ggtggagtaa agcccttgtg gagccaacgg tggaacaaga cctttcctcc caccatgaca actttggtac cgccccctgc agcgcggcta aagacgtttc gccgttgtgt agtggtggcc gaggtcggct gactgtctgt ggcacactgg actacttgtc cccggaaatg ctaattggca tgcctcacac cttcaagaca gatgtgtggt ccctcggtgt catgctggcc gagatgctaa ctggcgcacc acccttttac tgtgtgtcgc agcaggacac actcaacgcg attcgtgaag cagctcctca tttgggcgat gacgttcaaa tgtcatcgct ggcccgtgaa ttggtgctgg caatgttgca gaaaaacccg gatgcacgtc caacaatggc ggaagtgcta caacacccat ggacgagaca aaatggggag aagaagaaga agaagaagta a
Details for TrcrB.01480.b.B2.GE43003
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHDD DFQRLEVLGD GSYSVVVSAR HLPTQQLVAL KEVSRHRLRE LKLEKQLQWE INVHRTLRHP NVVRMLSYYI TPQTVVLVLE MCPGGTLLQK LKTMPGGRFD ERRASRYVRQ AARALTYLHG HGIAHRDLKL ENIFLDAHGV ARLGDFGWSK ALVEPTVEQD LSSHHDNFGT APCSAAKDVS PLCSGGRGRL TVCGTLDYLS PEMLIGMPHT FKTDVWSLGV MLAEMLTGAP PFYCVSQQDT LNAIREAAPH LGDDVQMSSL ARELVLAMLQ KNPDARPTMA EVLQHPWTRQ NGEK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgacg acttccagcg gctagaggtg ttgggcgacg gttcttactc cgtggtggtt tccgcccgcc acctacccac ccagcagctt gttgcgctga aggaagtatc gcgacaccgc ctccgtgagc tgaagttgga gaagcagttg caatgggaga tcaacgttca ccgcacgctg cgtcatccca atgtggttcg aatgctgagc tactatatca ccccgcagac cgttgtgctt gtgttggaga tgtgtccagg cgggacgctc ctgcagaaac tgaagacaat gcccggtgga aggtttgatg agcggcgggc atcacggtac gttcggcagg ccgcgcgggc gctaacgtac ctgcacgggc acggaattgc ccaccgtgac ttgaaactgg agaacatttt tttagacgcg cacggtgtgg cacggctggg agattttggg tggagtaaag cccttgtgga gccaacggtg gaacaagacc tttcctccca ccatgacaac tttggtaccg ccccctgcag cgcggctaaa gacgtttcgc cgttgtgtag tggtggccga ggtcggctga ctgtctgtgg cacactggac tacttgtccc cggaaatgct aattggcatg cctcacacct tcaagacaga tgtgtggtcc ctcggtgtca tgctggccga gatgctaact ggcgcaccac ccttttactg tgtgtcgcag caggacacac tcaacgcgat tcgtgaagca gctcctcatt tgggcgatga cgttcaaatg tcatcgctgg cccgtgaatt ggtgctggca atgttgcaga aaaacccgga tgcacgtcca acaatggcgg aagtgctaca acacccatgg acgagacaaa atggggagaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat