TrcrB.01480.a

serine/threonine protein kinase, putative

CENTER ID: TrcrB.01480.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TrcrB.01480.a.B2.GE40099 PK-like domain 130 430
TrcrB.01480.a.B3.GE40795 putative kinase domain 126 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tc00.1047053508479.150
RefSeq: XP_820852.1
UniProt: Q4E2L0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMMMPNEYVF FFLFFSFLIL GIEESHSTCT QRFFFTYTHR SKYLIYCINT RKYLFRNIHT YLFVFHWGRG SGKKSKKKKK KKMFLKLFKR DKKEKDEERS GEKASEKKVG NNNHMDKKAP PQEKVTKDDF ESLDVLGKGS FAYVVLVRRI GTNEYYAMKV VNKQGLLDHN RYRDVFVERN VLSRTNHPYL LKLYWTFQSE HKLFFVMEYM PGGDLDKYMN NLPSKQVDLF TAKLYAAEIL LALLFLHEHS VIYRDLKPEN ILLTGDGHCV LADFGLSKDF YNPKEGGDAS TKDMRANSFV GSPFYVAPDV LKQNEYTNAV DFWSFGILLY RMLCGRTPFN GKSMREVFDN ILYSDLRFPS SVQLPSEAKD LISRLLIKDA NRRIKGPEIK AHLFWTGINF DEVMERKVKP PKWVPIPSPE QVMAERAKAE GASTSAKNPG QLVNTPAQSS QLNSRQQQLF TGFSCTADNH LNKN
NT Sequence
atgatgatga tgcctaatga atatgttttc ttttttcttt tcttttcttt tttgattttg gggattgaag aaagtcatag tacctgcaca caacgattct tttttacata cacacaccgc agtaaatatc tcatttattg cataaacact cgtaaatatt tatttagaaa tatacatacg tatttatttg ttttccactg gggacgggga agtgggaaaa aaagcaaaaa aaaaaaaaaa aaaaaaatgt ttttgaagct tttcaaaagg gacaagaagg agaaggacga agagcgttcg ggcgaaaaag ccagcgagaa gaaggttggt aacaataacc atatggataa aaaggcacca ccgcaggaaa aagtgacaaa ggatgacttt gagagtctgg atgtacttgg gaaaggttct tttgcgtacg tagtccttgt gcgtcgcata ggcacgaacg aatattacgc catgaaagta gtgaacaaac aaggactact cgatcacaat cgttatcgcg atgtatttgt tgaacgcaat gtattgtcac gtaccaatca cccatatctt ttgaagcttt actggacctt tcagtcggag cataagttgt tttttgtgat ggaatatatg ccaggaggcg atttagacaa atatatgaac aaccttcctt caaaacaggt tgatcttttt acagcaaagc tttacgcggc tgaaattctt cttgcattat tatttctgca tgaacacagt gtcatttatc gtgatttgaa gccggagaac atacttttaa caggggatgg acattgtgtt ttggctgact ttggtctttc gaaggatttt tataacccaa aggagggtgg ggatgcatct acgaaagata tgcgggcaaa ttcttttgtt ggttcgccgt tttatgttgc gccggatgta ctcaaacaaa atgagtacac aaacgcggta gatttttggt cgtttggcat cttattgtac cgcatgcttt gtggacgtac acccttcaat ggaaagagca tgagggaggt ctttgacaac attctttact cggacctccg tttccccagc agcgtgcaac ttccatcgga ggcgaaggac ctgatcagcc gacttctcat aaaggacgcc aaccgccgca tcaagggccc ggagatcaaa gcacatcttt tttggacagg catcaacttt gacgaggtga tggagaggaa ggtgaagccg ccaaagtggg tgccaatccc atcgccagag caagtgatgg cagagcgggc gaaggcggag ggcgcatcca cctcggccaa gaatcccggg caacttgtca atacgcctgc gcagtcgtca caactgaact cgcggcagca gcagcttttc acggggtttt catgcacagc ggataaccac ttgaataaaa attag
Details for TrcrB.01480.a.B2.GE40099
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 36kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMF ESLDVLGKGS FAYVVLVRRI GTNEYYAMKV VNKQGLLDHN RYRDVFVERN VLSRTNHPYL LKLYWTFQSE HKLFFVMEYM PGGDLDKYMN NLPSKQVDLF TAKLYAAEIL LALLFLHEHS VIYRDLKPEN ILLTGDGHCV LADFGLSKDF YNPKEGGDAS TKDMRANSFV GSPFYVAPDV LKQNEYTNAV DFWSFGILLY RMLCGRTPFN GKSMREVFDN ILYSDLRFPS SVQLPSEAKD LISRLLIKDA NRRIKGPEIK AHLFWTGINF DEVMERKVKP PKWVPIPSPE QVMA
Validated NT Sequence
tctgccatca cttgctctgg cgatgggatt ggcacccact ttggcggctt caccttcctc tccatcacct cgtcaaagtt gatgcctgtc caaaaaagat gtgctttgat ctccgggccc ttgatgcggc ggttggcgtc ctttatgaga agtcggctga tcaggtcctt cgcctccgat ggaagttgca cgctgctggg gaaacggagg tccgagtaaa gaatgttgtc aaagacctcc ctcatgctct ttccattgaa gggtgtacgt ccacaaagca tgcggtacaa taagatgcca aacgaccaaa aatctaccgc gtttgtgtac tcattttgtt tgagtacatc cggcgcaaca taaaacggcg aaccaacaaa agaatttgcc cgcatatctt tcgtagatgc atccccaccc tcctttgggt tataaaaatc cttcgaaaga ccaaagtcag ccaaaacaca atgtccatcc cctgttaaaa gtatgttctc cggcttcaaa tcacgataaa tgacactgtg ttcatgcaga aataataatg caagaagaat ttcagccgcg taaagctttg ctgtaaaaag atcaacctgt tttgaaggaa ggttgttcat atatttgtct aaatcgcctc ctggcatata ttccatcaca aaaaacaact tatgctccga ctgaaaggtc cagtaaagct tcaaaagata tgggtgattg gtacgtgaca atacattgcg ttcaacaaat acatcgcgat aacgattgtg atcgagtagt ccttgtttgt tcactacttt catggcgtaa tattcgttcg tgcctatgcg acgcacaagg actacgtacg caaaagaacc tttcccaagt acatccagac tctcaaacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHFE SLDVLGKGSF AYVVLVRRIG TNEYYAMKVV NKQGLLDHNR YRDVFVERNV LSRTNHPYLL KLYWTFQSEH KLFFVMEYMP GGDLDKYMNN LPSKQVDLFT AKLYAAEILL ALLFLHEHSV IYRDLKPENI LLTGDGHCVL ADFGLSKDFY NPKEGGDAST KDMRANSFVG SPFYVAPDVL KQNEYTNAVD FWSFGILLYR MLCGRTPFNG KSMREVFDNI LYSDLRFPSS VQLPSEAKDL ISRLLIKDAN RRIKGPEIKA HLFWTGINFD EVMERKVKPP KWVPIPSPEQ VMAERAKAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattttgaga gtctggatgt acttgggaaa ggttcttttg cgtacgtagt ccttgtgcgt cgcataggca cgaacgaata ttacgccatg aaagtagtga acaaacaagg actactcgat cacaatcgtt atcgcgatgt atttgttgaa cgcaatgtat tgtcacgtac caatcaccca tatcttttga agctttactg gacctttcag tcggagcata agttgttttt tgtgatggaa tatatgccag gaggcgattt agacaaatat atgaacaacc ttccttcaaa acaggttgat ctttttacag caaagcttta cgcggctgaa attcttcttg cattattatt tctgcatgaa cacagtgtca tttatcgtga tttgaagccg gagaacatac ttttaacagg ggatggacat tgtgttttgg ctgactttgg tctttcgaag gatttttata acccaaagga gggtggggat gcatctacga aagatatgcg ggcaaattct tttgttggtt cgccgtttta tgttgcgccg gatgtactca aacaaaatga gtacacaaac gcggtagatt tttggtcgtt tggcatctta ttgtaccgca tgctttgtgg acgtacaccc ttcaatggaa agagcatgag ggaggtcttt gacaacattc tttactcgga cctccgtttc cccagcagcg tgcaacttcc atcggaggcg aaggacctga tcagccgact tctcataaag gacgccaacc gccgcatcaa gggcccggag atcaaagcac atcttttttg gacaggcatc aactttgacg aggtgatgga gaggaaggtg aagccgccaa agtgggtgcc aatcccatcg ccagagcaag tgatggcaga gcgggcgaag gcggagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for TrcrB.01480.a.B3.GE40795
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT KDDFESLDVL GKGSFAYVVL VRRIGTNEYY AMKVVNKQGL LDHNRYRDVF VERNVLSRIN HPYLLKLYWT FQSEHKLFFV MEYMPGGDLD KYMNNLPSKQ FDLFTAKLYA AEILLALLFL HEHSVIYRDL KPENILLTGD GHCVLADFGL SKDFYNPKEG GDASTKDMRA NSFVGSPFYV APDVLKQSEY TNAVDFWSFG ILLYRMLCGR TPFNGKSMRE VFDNILYSDL RFPSSVQLPS EAKDLISRLL IKDANRRIKG PEIKAHLFWT GINFDEVME
Validated NT Sequence
ttactcactt actccatcac ctcgtcaaag ttgatgcctg tccaaaaaag atgtgctttg atctccgggc ccttgatgcg gcggttggcg tcctttatga gaagtcggct gatcaggtcc ttcgcctccg atggaagttg cacgctgctg gggaaacgca ggtccgagta aagaatgttg tcaaacacct ccctcatgct ctttccattg aagggtgtac gtccacaaag catgcggtac aataagatgc caaacgacca aaaatccacc gcgtttgtgt actcactttg tttgagtaca tccggcgcaa cgtaaaacgg cgaaccaaca aaagaatttg cccgcatatc tttcgtagat gcatccccac cctcctttgg gttgtaaaaa tccttcgaaa gaccaaagtc agccaaaaca caatgtccat cccctgttaa aagtatgttc tccggcttca aatcacgata aatgacactg tgttcatgca gaaataataa tgcaagaaga atttcagccg cgtaaagctt tgctgtaaaa agatcaaact gttttgaagg aaggttgttc atatatttgt ctaaatcgcc tcctggcata tattccatca caaaaaacaa cttatgctcc gactgaaagg tccagtaaag cttcaaaaga tacgggtgat tgatacgtga caatacattg cgttcaacaa atacatcgcg ataacgattg tgatcgagta gtccttgttt gttcactact ttcatggcgt aatattcgtt cgtgcctatg cgacgcacaa ggactacgta cgcaaaagaa cctttcccaa gtacatccag actctcaaag tcatcctttg tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHTK DDFESLDVLG KGSFAYVVLV RRIGTNEYYA MKVVNKQGLL DHNRYRDVFV ERNVLSRTNH PYLLKLYWTF QSEHKLFFVM EYMPGGDLDK YMNNLPSKQV DLFTAKLYAA EILLALLFLH EHSVIYRDLK PENILLTGDG HCVLADFGLS KDFYNPKEGG DASTKDMRAN SFVGSPFYVA PDVLKQNEYT NAVDFWSFGI LLYRMLCGRT PFNGKSMREV FDNILYSDLR FPSSVQLPSE AKDLISRLLI KDANRRIKGP EIKAHLFWTG INFDEVME
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacaaagg atgactttga gagtctggat gtacttggga aaggttcttt tgcgtacgta gtccttgtgc gtcgcatagg cacgaacgaa tattacgcca tgaaagtagt gaacaaacaa ggactactcg atcacaatcg ttatcgcgat gtatttgttg aacgcaatgt attgtcacgt accaatcacc catatctttt gaagctttac tggacctttc agtcggagca taagttgttt tttgtgatgg aatatatgcc aggaggcgat ttagacaaat atatgaacaa ccttccttca aaacaggttg atctttttac agcaaagctt tacgcggctg aaattcttct tgcattatta tttctgcatg aacacagtgt catttatcgt gatttgaagc cggagaacat acttttaaca ggggatggac attgtgtttt ggctgacttt ggtctttcga aggattttta taacccaaag gagggtgggg atgcatctac gaaagatatg cgggcaaatt cttttgttgg ttcgccgttt tatgttgcgc cggatgtact caaacaaaat gagtacacaa acgcggtaga tttttggtcg tttggcatct tattgtaccg catgctttgt ggacgtacac ccttcaatgg aaagagcatg agggaggtct ttgacaacat tctttactcg gacctccgtt tccccagcag cgtgcaactt ccatcggagg cgaaggacct gatcagccga cttctcataa aggacgccaa ccgccgcatc aagggcccgg agatcaaagc acatcttttt tggacaggca tcaactttga cgaggtgatg gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat