SSGCID: Target Page

TrcrB.01391.a

Mitogen-activated protein kinase (EC 2.7.11.24)

CENTER ID:	TrcrB.01391.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.01391.a.B2.GE43850	kinase domain (Jensen)		1	359

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:Tc00.1047053510295.50
UniProt:	Q4CZ09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSEIEPHIL RKYEIQAQLG QGAYGIVWRA SDRRTHQVVA LKKIYDAFQN ATDAQRTFRE IMFLQALSHP NIIKLLHVHR ATNDKDIYLV FEYMETDLHV VIRANILEDI HKQFIIYQLL KTLKYLHSAE LLHRDMKPSN LLVNSDCSMK VADFGLARSI LSLEKEQVAR PALTDYIMTR WYRPPEILLG STRYTKGVDM WAVGCILAEL LLGRPIFPGR TTIKQLELII NVLGEPTPED IASTNSQFAE AMMKDIRRTH TATFAELLPK ASPDALDLVQ KLMRFNPNER LTAEQALEHP YVAAFHKVQD EPSAPAPVTI SLPDDTRFTM QEYRERLYQE IANARRRVRR EAAAALSSKN ESGGVVTRAH PPSSAAAPVA EERREKVRDA AVTHTSSSTV ARPNSSTATR TALANGASRT TLASRTSDAT RPAHHTRPVV SRHGLNGSRT MQK

NT Sequence

atgtcatctg aaatagagcc gcatattctc cgcaaatatg agattcaggc gcagttagga cagggggcgt acggcatcgt ttggcgtgcg tccgaccgcc gcacccatca ggtagtcgcc ctaaaaaaaa tatacgatgc gtttcaaaac gcaaccgacg cacagcgcac atttcgtgag attatgtttt tgcaggccct tagccacccg aacataatta aactgctgca tgttcaccgt gccactaacg ataaagacat ttaccttgtc tttgagtata tggagacaga cctgcatgtc gttatccgtg ccaacattct tgaggatatc cacaaacagt ttattattta tcaactactg aaaactttga agtatcttca ttctgcggag ttgctacacc gcgatatgaa gccatcaaat cttttggtca acagcgactg ctcaatgaag gttgcggatt ttggattagc gcggtcaatt ctctctctgg agaaggaaca agtggctcgc cccgccctca cggactacat catgacccgt tggtatcgac cgccagagat cctactgggc tcgacacgtt atacaaaggg ggtggacatg tgggctgtag gatgtattct tgctgaactc cttcttggaa gacccatatt ccctgggcgt acaacaataa agcagctgga gctcatcatc aacgttcttg gcgaaccgac accagaggat attgcgtcga caaactccca gtttgcggag gcgatgatga aagacattcg acgcacacat acggcgacgt ttgcggagtt gcttcctaaa gcctcgccgg acgcacttga cttggtgcaa aaacttatgc gattcaaccc caatgagcgc ctcaccgcag agcaggcgtt ggagcatcca tatgtggctg cttttcacaa ggtgcaggac gagccctcgg cacctgcgcc agtaactatc tcgcttccag atgacacacg ctttaccatg caggaatacc gtgagcgtct ctaccaggag attgcaaacg caagaagaag agtccgccga gaggcggccg cagccttatc ttcaaagaac gagtctggcg gcgtggtgac tcgggcgcat ccgccctcct ccgctgcggc ccccgtggcg gaagagaggc gggaaaaagt ccgtgacgcg gcagtcacgc acacatcgtc ttcgactgtt gctcggccca attcttcgac cgcaacacgt acggcgttgg cgaatggggc cagtcgaaca actcttgcaa gtagaacttc cgacgcgacg cggcctgcgc atcatactcg gcctgttgtt tcacgtcatg gacttaatgg ttcacgaacc atgcaaaagt ag

Details for TrcrB.01391.a.B2.GE43850

HARVESTED ON:	8/24/2020
SEQUENCED ON:	8/28/2020
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS SEIEPHILRK YEIQAQLGQG AYGIVWRASD RRTHQVVALK KIYDAFQNAT DAQRTFREIM FLQALSHPNI IKLLHVHRAT NDKDIYLVFE YMETDLHVVI RANILEDIHK QFIIYQLLKT LKYLHSAELL HRDMKPSNLL VNSDCSMKVA DFGLARSILS LEKEQVARPA LTDYIMTRWY RPPEILLGST RYTKGVDMWA VGCILAELLL GRPIFPGRTT IKQLELIINV LGEPTPEDIA STNSQFAEAM MKDIRRTHTA TFAELLPKAS PDALDLVQKL MRFNPNERLT AEQALEHPYV AAFHKVQDEP SAPAPVTISL PDDTRFTMQE YRERLYQEIA NARRRVRR

Validated NT Sequence

atggctcacc accaccacca ccatatgtca tctgaaatag agccgcatat tctccgcaaa tatgagattc aggcgcagtt aggacagggg gcgtacggca tcgtttggcg tgcgtccgac cgccgcaccc atcaggtagt cgccctaaag aaaatatacg atgcgtttca aaacgcaacc gacgcacagc gcacatttcg tgagattatg tttttgcagg cccttagcca cccgaacata ataaaactgc tgcatgttca ccgtgccact aacgataaag atatttacct tgtctttgag tatatggaga cagacctgca tgttgttatc cgtgccaaca ttcttgagga tatccacaaa cagtttatta tttatcaact actgaaaacg ttgaagtacc tccattctgc ggagttgcta caccgcgata tgaagccatc aaatcttttg gtcaacagcg actgctcaat gaaggttgcg gattttggat tagcgcggtc aattctctct ctggagaagg aacaagtggc tcgccccgcc ctcacggatt acatcatgac ccgttggtat cgaccgccag agatcctact gggctcgaca cgctatacaa agggggtgga catgtgggct gtaggatgta ttcttgctga gctccttctt ggaagaccca tattccctgg gcgtacaaca ataaagcagc tggagcttat catcaatgtt cttggcgaac cgacaccaga ggatattgcg tcgacaaact cccagtttgc ggaggcgatg atgaaggaca ttcgacgcac acatacggcg acttttgcgg agttgcttcc taaagcctcg ccggacgcac ttgacttggt gcaaaaactt atgcgattca accccaatga gcgcctcacc gcagagcagg cgttggagca tccatatgtg gctgcttttc acaaggtgca ggacgagccc tcggcacctg cgccagtaac catctcgctt ccagatgaca cacgctttac catgcaggaa taccgtgagc gtctctacca ggagattgca aacgcaagaa gaagagtccg ccgagag

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcat ctgaaataga gccgcatatt ctccgcaaat atgagattca ggcgcagtta ggacaggggg cgtacggcat cgtttggcgt gcgtccgacc gccgcaccca tcaggtagtc gccctaaaaa aaatatacga tgcgtttcaa aacgcaaccg acgcacagcg cacatttcgt gagattatgt ttttgcaggc ccttagccac ccgaacataa ttaaactgct gcatgttcac cgtgccacta acgataaaga catttacctt gtctttgagt atatggagac agacctgcat gtcgttatcc gtgccaacat tcttgaggat atccacaaac agtttattat ttatcaacta ctgaaaactt tgaagtatct tcattctgcg gagttgctac accgcgatat gaagccatca aatcttttgg tcaacagcga ctgctcaatg aaggttgcgg attttggatt agcgcggtca attctctctc tggagaagga acaagtggct cgccccgccc tcacggacta catcatgacc cgttggtatc gaccgccaga gatcctactg ggctcgacac gttatacaaa gggggtggac atgtgggctg taggatgtat tcttgctgaa ctccttcttg gaagacccat attccctggg cgtacaacaa taaagcagct ggagctcatc atcaacgttc ttggcgaacc gacaccagag gatattgcgt cgacaaactc ccagtttgcg gaggcgatga tgaaagacat tcgacgcaca catacggcga cgtttgcgga gttgcttcct aaagcctcgc cggacgcact tgacttggtg caaaaactta tgcgattcaa ccccaatgag cgcctcaccg cagagcaggc gttggagcat ccatatgtgg ctgcttttca caaggtgcag gacgagccct cggcacctgc gccagtaact atctcgcttc cagatgacac acgctttacc atgcaggaat accgtgagcg tctctaccag gagattgcaa acgcaagaag aagagtccgc cgagaggcgg ccgcagcctt atcttcaaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat