SSGCID: Target Page

TrcrB.01238.a

seryl-tRNA synthetase

CENTER ID:	TrcrB.01238.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.01238.a.B1.GE42635	full length		1	477

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.01238.a.B1.PW38567	full length		1	477

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.506777.80
UniProt:	Q4DC68

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLDVQLFRD EKGADIIRES QRRRFADPTI VDAVLEVDKS WRRTQFMTDA YNKLINTCSK LVGAKKKEKE ADGDTSDIPE SIRAACKEGE LAPDQLKGLC VLQLKQLSKD LSEQVSVLAK EAAELVAKRD GLVLSVGNII HESVPVAQDE DTGNVVVRTF GDVTKRAKLN HVTIMERLGM MDTTKTVTSM AGGRAYVLKG GLVQLQLALL SYGMNFLVSR GYTPLYPPFF LNKDAMSEVA QLSQFDEELY KVTGDGEEKY LIATSEMPIA AYHRGKWFTE LKEPLKYAGM SSCFRKEAGA HGRDTLGIFR VHQFDKLEQF LVCSPREEES WRHLEEMIRT SEEFCESLGL PYRTINICSG ALNNAAAKKY DVEAWFPASG AFRELVSCSN CTDYQARGVN CRFGPNLRNV SANNVKEYCH MLNGTLCAIT RTMCCICENY QTEEGVVVPD VLRPYMMGLE MLKFADNVPS AEETESK

NT Sequence

atgggactcg atgttcagct gttccgcgat gaaaaggggg cagatattat ccgcgagtca caaagacggc gatttgcgga tcccaccatt gtggacgccg tgctggaggt agacaagagc tggcggcgca cacaatttat gacggatgcc tacaataagt taatcaatac atgcagcaag cttgtcggtg cgaagaagaa ggaaaaggag gcggacgggg acacatccga cattcctgag tcgatccgcg cagcctgcaa ggagggggag cttgccccgg atcagctgaa aggcctttgt gtgctgcagc taaagcagct gtcaaaagac ctctcggaac aggtgtcggt tcttgcgaag gaggcagcag agttggtggc aaagcgtgat ggccttgtgc ttagtgtcgg caacatcatt catgaatcgg ttcccgtggc gcaagatgag gatacgggca atgtcgttgt acgcaccttt ggtgatgtga cgaaaagggc aaaactgaat catgtgacga tcatggagcg tctcggtatg atggatacaa caaagacagt tacctccatg gcgggtggga gggcgtacgt gctgaagggt ggactcgttc agctccaatt ggcgctcttg tcgtacggga tgaactttct tgttagcaga ggttacaccc cgttgtaccc gcccttcttc cttaacaagg atgccatgtc agaggtggcg cagctctctc aattcgatga ggaattgtac aaagtaacgg gtgacggtga ggaaaagtat ttgattgcca cgagcgagat gcctattgcg gcgtaccacc ggggtaaatg gttcacggag ctgaaggagc cgttgaagta cgctggaatg tcgagttgct tccggaagga ggccggcgct cacggccgtg acacgctcgg tatcttccgt gtacatcagt ttgacaagtt ggaacaattt ctggtgtgtt ccccacggga ggaggagtcc tggcggcatc ttgaggagat gattcgcacc tccgaggaat tctgcgagag ccttggactg ccgtaccgca ccatcaacat ttgctctggt gcgttgaata acgccgcggc aaagaaatat gatgttgagg catggttccc cgcatcaggc gccttccgtg agttggtatc gtgttctaac tgcacggact atcaggcgcg cggggtgaat tgccgctttg gtccaaactt gcgcaacgtg tcggccaaca acgtcaagga gtactgtcat atgttgaatg gtacattgtg cgctattaca aggacgatgt gttgcatctg cgagaactat cagacagagg agggtgttgt cgttccagat gtgcttcgtc catacatgat gggcctggag atgctgaagt ttgctgataa cgtgccatcc gccgaggaaa cggagtcaaa atag

Details for TrcrB.01238.a.B1.GE42635

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG LDVQLFRDEK GADIIRESQR RRFADPTIVD AVLEVDKSWR RTQFMTDAYN KLINTCSKLV GAKKKEKEAD GDTSDIPESI RAACNEGELA PDQLKGLCVL QLKQLSKDLS EQVSVLAKEA AELVAKRDGL VLSVGNIIHE SVPVAQDEDT GNVVVRTFGD VTKRAKLNHV TIMERLGMMD TTKTVTSMAG GRAYVLKGGL VQLQLALLSY GMNFLVSRGY TPLYPPFFLN KDAMSEVAQL SQFDEELYKV TGDGEEKYLI ATSEMPIAAY HRGKWFTELK EPLKYAGMSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFLV CSPREEESWR HLEEMIRTSE EFCESLGLPY RTINICSGAL NNAAAKKYDV EAWFPASGAF RELVSCSNCT DYQARGVNCR FGPNLRNVSA NNVKEYCHML NGTLCAITRT MCCICENYQT EEGVVIPDVL RPYMMGLEML KFADNV

Validated NT Sequence

ggcacgttat cagcaaactt cagcatctcc aggcccatca tgtatggacg aagcacatct ggaatgacaa caccctcctc tgtctgatag ttctcgcaga tgcaacacat cgtcctcgta atagcgcaca atgtaccatt caacatatga cagtactcct tgacgttgtt ggccgacacg ttgcgcaagt ttggaccgaa gcggcaattc accccgcgcg cctgatagtc cgtgcagtta gaacacgata ccaactcacg gaaggcgcct gatgcgggga accatgcctc aacatcatat ttctttgccg cggcgttatt caacgcacca gagcaaatgt tgatggtgcg gtacggcagt ccaaggctct cgcagaattc ctcggaggtg cgaatcatct cctcaagatg ccgccaggac tcctcctccc gtggggaaca caccagaaat tgttccaact tgtcaaactg atgtacacgg aagataccga gcgtgtcacg gccgtgagcg ccggcctcct tccggaagca actcgacatt ccagcgtact tcaacggctc cttcagctcc gtgaaccatt taccccggtg gtacgccgca ataggcatct cgctcgtggc aatcaaatac ttttcctcac cgtcacccgt tactttgtac aattcctcat cgaattgaga gagctgcgcc acctctgaca tggcatcctt gttaaggaag aagggcgggt acaacggggt gtaacctctg ctaacaagaa agttcatccc gtacgacaag agcgccaatt ggagctgaac gagtccaccc ttcagcacgt acgccctccc acccgccatg gaggtaactg tctttgttgt atccatcata ccgagacgct ccatgatcgt cacatgattc agttttgccc ttttcgtcac atcaccaaag gtgcgtacaa cgacattgcc cgtatcctca tcttgcgcca cgggaaccga ttcatgaatg atgttgccga cactaagcac aaggccatca cgctttgcca ccaactctgc tgcctccttc gcaagaaccg acacctgttc cgagaggtcc tttgacagct gctttagctg cagcacacaa aggcctttca gctgatccgg ggcaagctcc ccctcattgc aggctgcgcg gatcgactca ggaatgtcgg atgtgtcccc gtctgcctcc ttttctttct tcttcgcacc gacaagcttg ctgcatgtat tgattaactt attgtaggca tccgtcataa attgtgtgcg ccgccagctc ttgtctacct ccagcacggc atccacaatg gtgggatccg caaatcgccg tcgttgtgac tcgcggataa tatctgcccc cttttcatcg cggaacagct gaacatcgag tcccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHMG LDVQLFRDEK GADIIRESQR RRFADPTIVD AVLEVDKSWR RTQFMTDAYN KLINTCSKLV GAKKKEKEAD GDTSDIPESI RAACKEGELA PDQLKGLCVL QLKQLSKDLS EQVSVLAKEA AELVAKRDGL VLSVGNIIHE SVPVAQDEDT GNVVVRTFGD VTKRAKLNHV TIMERLGMMD TTKTVTSMAG GRAYVLKGGL VQLQLALLSY GMNFLVSRGY TPLYPPFFLN KDAMSEVAQL SQFDEELYKV TGDGEEKYLI ATSEMPIAAY HRGKWFTELK EPLKYAGMSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFLV CSPREEESWR HLEEMIRTSE EFCESLGLPY RTINICSGAL NNAAAKKYDV EAWFPASGAF RELVSCSNCT DYQARGVNCR FGPNLRNVSA NNVKEYCHML NGTLCAITRT MCCICENYQT EEGVVVPDVL RPYMMGLEML KFADNVPSAE ETESK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggac tcgatgttca gctgttccgc gatgaaaagg gggcagatat tatccgcgag tcacaaagac ggcgatttgc ggatcccacc attgtggacg ccgtgctgga ggtagacaag agctggcggc gcacacaatt tatgacggat gcctacaata agttaatcaa tacatgcagc aagcttgtcg gtgcgaagaa gaaggaaaag gaggcggacg gggacacatc cgacattcct gagtcgatcc gcgcagcctg caaggagggg gagcttgccc cggatcagct gaaaggcctt tgtgtgctgc agctaaagca gctgtcaaaa gacctctcgg aacaggtgtc ggttcttgcg aaggaggcag cagagttggt ggcaaagcgt gatggccttg tgcttagtgt cggcaacatc attcatgaat cggttcccgt ggcgcaagat gaggatacgg gcaatgtcgt tgtacgcacc tttggtgatg tgacgaaaag ggcaaaactg aatcatgtga cgatcatgga gcgtctcggt atgatggata caacaaagac agttacctcc atggcgggtg ggagggcgta cgtgctgaag ggtggactcg ttcagctcca attggcgctc ttgtcgtacg ggatgaactt tcttgttagc agaggttaca ccccgttgta cccgcccttc ttccttaaca aggatgccat gtcagaggtg gcgcagctct ctcaattcga tgaggaattg tacaaagtaa cgggtgacgg tgaggaaaag tatttgattg ccacgagcga gatgcctatt gcggcgtacc accggggtaa atggttcacg gagctgaagg agccgttgaa gtacgctgga atgtcgagtt gcttccggaa ggaggccggc gctcacggcc gtgacacgct cggtatcttc cgtgtacatc agtttgacaa gttggaacaa tttctggtgt gttccccacg ggaggaggag tcctggcggc atcttgagga gatgattcgc acctccgagg aattctgcga gagccttgga ctgccgtacc gcaccatcaa catttgctct ggtgcgttga ataacgccgc ggcaaagaaa tatgatgttg aggcatggtt ccccgcatca ggcgccttcc gtgagttggt atcgtgttct aactgcacgg actatcaggc gcgcggggtg aattgccgct ttggtccaaa cttgcgcaac gtgtcggcca acaacgtcaa ggagtactgt catatgttga atggtacatt gtgcgctatt acaaggacga tgtgttgcat ctgcgagaac tatcagacag aggagggtgt tgtcgttcca gatgtgcttc gtccatacat gatgggcctg gagatgctga agtttgctga taacgtgcca tccgccgagg aaacggagtc aaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for TrcrB.01238.a.B1.PW38567

PURIFICATION DATe:	11/13/2018
CONCENTRATION:	42.44mg/ml
OBSERVED MW:	56kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	6
VIAL VOLUME:	210µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGAC TCGATGTTCA GCTGTTCCGC GATGAAAAGG GGGCAGATAT TATCCGCGAG TCACAAAGAC GGCGATTTGC GGATCCCACC ATTGTGGACG CCGTGCTGGA GGTAGACAAG AGCTGGCGGC GCACACAATT TATGACGGAT GCCTACAATA AGTTAATCAA TACATGCAGC AAGCTTGTCG GTGCGAAGAA GAAGGAAAAG GAGGCGGACG GGGACACATC CGACATTCCT GAGTCGATCC GCGCAGCCTG CAAGGAGGGG GAGCTTGCCC CGGATCAGCT GAAAGGCCTT TGTGTGCTGC AGCTAAAGCA GCTGTCAAAA GACCTCTCGG AACAGGTGTC GGTTCTTGCG AAGGAGGCAG CAGAGTTGGT GGCAAAGCGT GATGGCCTTG TGCTTAGTGT CGGCAACATC ATTCATGAAT CGGTTCCCGT GGCGCAAGAT GAGGATACGG GCAATGTCGT TGTACGCACC TTTGGTGATG TGACGAAAAG GGCAAAACTG AATCATGTGA CGATCATGGA GCGTCTCGGT ATGATGGATA CAACAAAGAC AGTTACCTCC ATGGCGGGTG GGAGGGCGTA CGTGCTGAAG GGTGGACTCG TTCAGCTCCA ATTGGCGCTC TTGTCGTACG GGATGAACTT TCTTGTTAGC AGAGGTTACA CCCCGTTGTA CCCGCCCTTC TTCCTTAACA AGGATGCCAT GTCAGAGGTG GCGCAGCTCT CTCAATTCGA TGAGGAATTG TACAAAGTAA CGGGTGACGG TGAGGAAAAG TATTTGATTG CCACGAGCGA GATGCCTATT GCGGCGTACC ACCGGGGTAA ATGGTTCACG GAGCTGAAGG AGCCGTTGAA GTACGCTGGA ATGTCGAGTT GCTTCCGGAA GGAGGCCGGC GCTCACGGCC GTGACACGCT CGGTATCTTC CGTGTACATC AGTTTGACAA GTTGGAACAA TTTCTGGTGT GTTCCCCACG GGAGGAGGAG TCCTGGCGGC ATCTTGAGGA GATGATTCGC ACCTCCGAGG AATTCTGCGA GAGCCTTGGA CTGCCGTACC GCACCATCAA CATTTGCTCT GGTGCGTTGA ATAACGCCGC GGCAAAGAAA TATGATGTTG AGGCATGGTT CCCCGCATCA GGCGCCTTCC GTGAGTTGGT ATCGTGTTCT AACTGCACGG ACTATCAGGC GCGCGGGGTG AATTGCCGCT TTGGTCCAAA CTTGCGCAAC GTGTCGGCCA ACAACGTCAA GGAGTACTGT CATATGTTGA ATGGTACATT GTGCGCTATT ACAAGGACGA TGTGTTGCAT CTGCGAGAAC TATCAGACAG AGGAGGGTGT TGTCGTTCCA GATGTGCTTC GTCCATACAT GATGGGCCTG GAGATGCTGA AGTTTGCTGA TAACGTGCCA TCCGCCGAGG AAACGGAGTC AAAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT