TrcrB.01031.a

Glucose-6-phosphate 1-dehydrogenase (G6PDH1)

CENTER ID: TrcrB.01031.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.01031.a.AE1.PW39159 full length 1 555

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.509287.50
UniProt: Q1WBU6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGSENRCRF QKEVDKFFDT LRERTLRDHP QELCEYLMEN AKKVAAELRG EVCERIPDAV SPELRSRALT IVVLGASGDL AKKKTFPALF QLYCNGMLPR DVNILGYARS TMEDVEKWKK DTLAGFFTRL DERGCHVGNF LRRISYMTGS YDRDEDFARL NERILRMEEA FQGPEKGGNR LFYLALPPSV FVGVCRGLSK GAMQKPELGW VRLIVEKPFG RDTETSEQLS NQLKPLFNER QVFRIDHYLG KEMVQNIIVT RFANRVFSAL WNSNSIACVQ ITFKEKIGTA GRGGYFDSIG IIRDVIQNHL TQILSLLTME KPRSLSAEDI RDEKVQVLRQ VVPANPAECV LGQYTASADG STPGYLDDPS VPKGSHCPTF AVLRLHVNND RWHGVPFIIR AGKALEERLL DIRIQFKDEI RPFGESTQRN ELVIRAQPSE AMYLKLTAKT PGLLNDTHQT ELDLTYERRY DVTLPDAYES LIHEALLGNS TNFVRVDELD AAWRIYTPLL HAIDRGEVKV LPYAAGSCGP EEAQEFIRIS GYKTTNGNAY KCSNA
NT Sequence
atgagtggat cggagaatcg ctgccgcttc caaaaggagg tggacaaatt ttttgatacg cttcgagaac gaacgctgcg ggaccacccg caagaattat gtgaatattt aatggagaac gccaaaaagg ttgcagcgga attgaggggg gaagtgtgcg aacgcatccc cgatgccgtc tcaccggaat tgcgaagtcg ggccctcacc atcgtggtac tcggtgcaag cggggacttg gccaaaaaga agacctttcc ggcgcttttt cagttatact gcaacggcat gctacctcgg gatgtcaaca ttctcgggta tgcccgctcc acaatggagg atgtggaaaa gtggaaaaag gacactcttg ccggattctt tactcgtctg gatgagcgcg gatgtcatgt gggtaacttt ttgagacgga tcagttacat gaccgggtcg tatgatcgcg acgaggactt tgcacgcctg aacgagagga ttcttcggat ggaagaggcc ttccaggggc cggaaaaagg gggaaatcga cttttttacc ttgcgctgcc accttccgtt tttgtgggcg tttgccgggg tctcagcaaa ggggcgatgc aaaagccaga attgggttgg gtccgtctca ttgttgagaa gccctttggc cgggacacgg agacctccga gcagctctca aatcagctga agcctctctt caatgaacgc caggtattcc gcattgacca ctacttgggg aaggaaatgg tgcagaatat tattgtcacc cgttttgcta accgcgtttt tagcgcgctg tggaacagta acagcattgc atgcgtccaa attacattca aggagaaaat cggcacggcg ggacgtggtg ggtactttga cagtattgga atcatccgtg acgtgatcca gaaccacctc acacagattc tttctttatt gacgatggaa aagccgcgtt cactcagtgc tgaggacatc cgtgatgaaa aggtccaagt tctcagacaa gtggtccctg cgaacccagc agagtgtgtg ctgggtcagt acacggcctc cgcagatggt tctacacctg gctaccttga cgacccctct gtaccgaagg gcagccattg ccccaccttt gctgtgcttc gactgcacgt gaacaatgac aggtggcacg gtgtcccttt tatcatccgg gccggaaagg cactcgaaga gcgtctgctt gatatccgta ttcagttcaa ggacgaaata cgtcccttcg gtgaaagcac gcagcgcaat gaacttgtga tccgcgccca gccgtcggag gcgatgtatc tgaaactcac tgcaaagaca ccggggctgc tgaacgatac ccatcagacg gaacttgacc tcacgtacga gcggcgctac gacgttacac ttccggatgc gtatgagagt cttattcacg aagctcttct cgggaactcg accaactttg ttcgtgtaga cgagttggac gctgcatggc ggatttacac accgcttctc cacgccattg atcgcgggga ggtgaaggta ctgccctacg cggcgggttc gtgcgggcca gaggaagccc aggagtttat tcgaatttcc ggatataaga caacaaatgg caacgcatat aagtgctcaa acgcgtaa
Details for TrcrB.01031.a.AE1.PW39159
PURIFICATION DATe: 10/24/2022
CONCENTRATION: 19.81mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: Rosetta2 (DE3)
VIAL COUNT (approx.): 27
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MSGSENRCRF QKEVDKFFDT LRERTLRDHP QELCEYLMEN AKKVAAELRG EVCERIPDAV SPELRSRALT IVVLGASGDL AKKKTFPALF QLYCNGMLPR DVNILGYARS TMEDVEKWKK DTLAGFFTRL DERGCHVGNF LRRISYMTGS YDRDEDFARL NERILRMEEA FQGPEKGGNR LFYLALPPSV FVGVCRGLSK GAMQKPELGW VRLIVEKPFG RDTETSEQLS NQLKPLFNER QVFRIDHYLG KEMVQNIIVT RFANRVFSAL WNSNSIACVQ ITFKEKIGTA GRGGYFDSIG IIRDVIQNHL TQILSLLTME KPRSLSAEDI RDEKVQVLRQ VVPANPAECV LGQYTASADG STPGYLDDPS VPKGSHCPTF AVLRLHVNND RWHGVPFIIR AGKALEERLL DIRIQFKDEI RPFGESTQRN ELVIRAQPSE AMYLKLTAKT PGLLNDTHQT ELDLTYERRY DVTLPDAYES LIHEALLGNS TNFVRVDELD AAWRIYTPLL HAIDRGEVKV LPYAAGSCGP EEAQEFIRIS GYKTTNGNAY KCSNAGHHHH HH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GAGTGGATCG GAGAATCGCT GCCGCTTCCA AAAGGAGGTG GACAAATTTT TTGATACGCT TCGAGAACGA ACGCTGCGGG ACCACCCGCA AGAATTATGT GAATATTTAA TGGAGAACGC CAAAAAGGTT GCAGCGGAAT TGAGGGGGGA AGTGTGCGAA CGCATCCCCG ATGCCGTCTC ACCGGAATTG CGAAGTCGGG CCCTCACCAT CGTGGTACTC GGTGCAAGCG GGGACTTGGC CAAAAAGAAG ACCTTTCCGG CGCTTTTTCA GTTATACTGC AACGGCATGC TACCTCGGGA TGTCAACATT CTCGGGTATG CCCGCTCCAC AATGGAGGAT GTGGAAAAGT GGAAAAAGGA CACTCTTGCC GGATTCTTTA CTCGTCTGGA TGAGCGCGGA TGTCATGTGG GTAACTTTTT GAGACGGATC AGTTACATGA CCGGGTCGTA TGATCGCGAC GAGGACTTTG CACGCCTGAA CGAGAGGATT CTTCGGATGG AAGAGGCCTT CCAGGGGCCG GAAAAAGGGG GAAATCGACT TTTTTACCTT GCGCTGCCAC CTTCCGTTTT TGTGGGCGTT TGCCGGGGTC TCAGCAAAGG GGCGATGCAA AAGCCAGAAT TGGGTTGGGT CCGTCTCATT GTTGAGAAGC CCTTTGGCCG GGACACGGAG ACCTCCGAGC AGCTCTCAAA TCAGCTGAAG CCTCTCTTCA ATGAACGCCA GGTATTCCGC ATTGACCACT ACTTGGGGAA GGAAATGGTG CAGAATATTA TTGTCACCCG TTTTGCTAAC CGCGTTTTTA GCGCGCTGTG GAACAGTAAC AGCATTGCAT GCGTCCAAAT TACATTCAAG GAGAAAATCG GCACGGCGGG ACGTGGTGGG TACTTTGACA GTATTGGAAT CATCCGTGAC GTGATCCAGA ACCACCTCAC ACAGATTCTT TCTTTATTGA CGATGGAAAA GCCGCGTTCA CTCAGTGCTG AGGACATCCG TGATGAAAAG GTCCAAGTTC TCAGACAAGT GGTCCCTGCG AACCCAGCAG AGTGTGTGCT GGGTCAGTAC ACGGCCTCCG CAGATGGTTC TACACCTGGC TACCTTGACG ACCCCTCTGT ACCGAAGGGC AGCCATTGCC CCACCTTTGC TGTGCTTCGA CTGCACGTGA ACAATGACAG GTGGCACGGT GTCCCTTTTA TCATCCGGGC CGGAAAGGCA CTCGAAGAGC GTCTGCTTGA TATCCGTATT CAGTTCAAGG ACGAAATACG TCCCTTCGGT GAAAGCACGC AGCGCAATGA ACTTGTGATC CGCGCCCAGC CGTCGGAGGC GATGTATCTG AAACTCACTG CAAAGACACC GGGGCTGCTG AACGATACCC ATCAGACGGA ACTTGACCTC ACGTACGAGC GGCGCTACGA CGTTACACTT CCGGATGCGT ATGAGAGTCT TATTCACGAA GCTCTTCTCG GGAACTCGAC CAACTTTGTT CGTGTAGACG AGTTGGACGC TGCATGGCGG ATTTACACAC CGCTTCTCCA CGCCATTGAT CGCGGGGAGG TGAAGGTACT GCCCTACGCG GCGGGTTCGT GCGGGCCAGA GGAAGCCCAG GAGTTTATTC GAATTTCCGG ATATAAGACA ACAAATGGCA ACGCATATAA GTGCTCAAAC GCGGGGCACC ACCATCATCA TCATTAACGG ATCCGAATTC GAGCTCCGTC GACAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T