SSGCID: Target Page

TrcrB.00825.d

asparaginyl-tRNA synthetase, putative

CENTER ID:	TrcrB.00825.d
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.00825.d.B1.GE42634	full length		1	745

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.506559.350
UniProt:	Q4E595

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAFRLIFERR LAPLSNSCRR CCRTMFSGPT PRITSVTRSG LRGYVLWQSP PHGSCICPAL SSSRAFIHSP ERTTMASSEE LLESLQRIVG LDEKNSKDLS TKPERVQDLL GFFANQNMTP ESPREQKVML FNLWAKVKLP RHRDLLADYV KRKKLDSTQK VDAAIRFVDA MKEDALFDAA KFEKECGVDV VVSKDDTLRL VKTALAEEDL KALKTSWVKN PNMLLGKMKR VPGLQWADFA LVKAILEELV PPMIKDVVID EAEPKTEEKT TSPPRTKGAE VAATQKCPRN ADWKCVVDLH SLHRIGDLSS MQEGTKVRVV GWAHRVRHQS RLSFVVLRDG SGYIQAVFNG ETEPFHRETS LAIRGTLKYE PKAATELQPP YELHVDEYAI IGNSDGSIEN AITPESSPDK LLDDRHLVLR GTYGSTTIKV RHELIRAFRE YCWSVNMFEV TPPTLVQAEC EGGSTLFDVI YYGTTAHLTQ SSQLYLETCT SSVGDVYCIL PSYRAEKSKT RRHLSEYTHL EVEYSICDFE LFLSKLEDLI VGAFNRTIER AGDLIAFMNP EQLVDPKANP FDPNNWKFAP KKPFRRLKYA DAIKLCNDNG ILNPETNAPF QFGDDITDQP ERAMVAILGE MVFMTHFPAV MKSFYMARDP EDPTLTESVD VLAPGIGEIV GGSMRMWDHE ELIEAYRRKD LDPSVYYWYT EQRKYGSTPH GGFGLGMERF LVWLLNLDSV REACLFPRYM GRCKP

NT Sequence

atggcctttc gacttatttt tgaacgtcgg ttggcgccac tctcgaacag ttgtaggcgt tgctgccgca cgatgttttc tggtcccacg ccgcgaatta cttctgtgac gcgtagtgga ctgagaggat atgtattgtg gcaatctcca cctcatggga gttgcatctg ccctgcgctt tcctcttcta gagcttttat tcactcgcca gaaaggacaa ctatggcttc ttcggaggaa ttgcttgaga gccttcaacg gattgttggc ttagatgaga agaactccaa ggacctgtct acaaaaccag aacgcgtgca agacctcctt ggcttctttg cgaatcaaaa catgacacca gagtcccctc gcgagcaaaa agtgatgctt tttaacttgt gggccaaggt taagcttccc aggcaccgcg atcttcttgc agactacgtg aagaggaaaa agctggattc aacccaaaag gtggatgccg ccatacgctt tgtggatgcc atgaaggaag atgctctctt tgatgctgca aaatttgaaa aggaatgtgg cgtggacgtg gttgtctcga aagatgatac attgcggctc gtaaagacgg cattggcaga ggaagacctc aaggcattga aaacgtcatg ggtgaaaaac ccgaacatgc tgcttggcaa gatgaagcgg gtaccgggtt tgcaatgggc agactttgct ctggtgaagg ccatccttga ggagttggta ccgcccatga ttaaggatgt tgtcattgat gaggctgaac ccaagacaga ggagaagacc acctctccac ctcgcacgaa aggcgctgaa gtggcagcga cgcaaaagtg cccacgcaac gcggattgga aatgtgttgt ggatctgcat tcactccatc gcatcggtga tttatcttct atgcaggaag gtaccaaggt gcgtgttgtg ggatgggcac accgagttcg tcatcaatcg cgtctttcat ttgtggtact gcgtgatgga tctgggtaca ttcaagccgt attcaatggt gaaacagaac cctttcaccg cgaaacgtct ttggcaattc gcggtacact gaagtacgaa cccaaggctg ctactgaatt gcagccacca tacgagcttc atgtggatga atacgccatt attggtaact cggatggcag tattgagaac gctattaccc ctgaaagctc ccctgataaa ttgttggatg atcgtcattt ggtgcttcgt ggaacctatg gctcaacaac cattaaagtt cgtcacgaat taattcgagc ttttcgtgaa tactgttggt ccgtaaatat gtttgaagta acaccaccaa ctcttgtaca ggctgagtgt gaagggggat ctacgctttt tgatgtcatt tactacggaa cgacggctca ccttacacag tcttcccagt tataccttga gacatgcact tcttcagtgg gagatgtgta ctgtattctt cccagttatc gagctgagaa atccaagacg aggcgtcact tgagtgaata cacacatttg gaggttgagt acagtatctg cgactttgag cttttcctct caaaactgga agatcttatt gtgggagcat tcaaccgaac aattgagcgg gcgggagatt tgatcgcctt tatgaatccg gaacagttgg tggatccaaa ggcaaaccca tttgatccca acaactggaa gtttgcaccc aagaagccgt ttaggcgtct caagtatgct gacgccatta aattatgcaa tgataacggc attttgaacc cagagacaaa tgcaccattc caatttggtg acgacatcac ggatcagccg gagcgggcca tggtggcgat attgggggag atggtgttca tgacacattt tcctgcggta atgaagtctt tctacatggc ccgagaccca gaggatccga cacttacaga gtccgtggac gttttggctc ctggtattgg ggaaattgtc ggcggctcta tgcgtatgtg ggatcatgaa gaactgatag aggcgtacag gcgtaaggac cttgacccaa gtgtgtatta ttggtacacg gagcagcgca agtatggctc caccccacat ggaggctttg gtttgggaat ggagcgcttc ttggtgtggc tgctgaactt ggactcggtc agggaggcat gtcttttccc gcgttacatg ggaagatgca agccgtag

Details for TrcrB.00825.d.B1.GE42634

HARVESTED ON:	8/5/2018
SEQUENCED ON:	8/8/2018
EXPECTED MW:	86kDa
OBSERVED MW:	86kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA FRLIFERRLA PLSNSCRRCC RTMFSGPTPR ITSVTRSGLR GYVLWQSPPH GSCICPALSS SRAFIHSPER TTMASSEELL ESLQRIVGLD EKNSKDLSTK PERVQDLLGF FANQNMTPES PREQKVMLFN LWAKVKLPRH RDLLADYVKR KKLDSTQKVD AAIRFVDAMK EDALFDAAKF EKECGVDVVV SKDDTLRLVK TALAEEDLKA LKTSWVKNPN MLLGKMKRVP GLQWADFALV KAILEELVPP MIKDVVIDEA EPKTEEKTTS PPRTKGAEVA ATQKCPRNAD WKCVVDLHSL HRIGDLSSMQ EGTKVRVVGW AHRVRHQSRL SFVVLRDGSG YIQAVFNGET EPFHRETSLA IRGTLKYEPK AATELQPPYE LHVDEYAIIG NSDGSIENAI TPESSPDKLL DDRHLVLRGT YGSTTIKVRH ELIRAFREYC WSVNMFEVTP PTLVQAECEG GSTLFDVIYY GTTAHLTQSS QLYLETCTSS VGDVYCILPS YRAEKSKTRR HLSEYTHLEV EYSICDFELF LSKLEDLIVG AFNRTIERAG DLIAFMNPEQ LVDPKANPFD PNNWKFAPKK PFRRLKYADA IKLCNDNGIL NPETNAPFQF GDDITDQPER AMVAILGEMV FMTHFPAVMK SFYMARDPED PTLTESVDVL APGIGEIVGG SMRMWDHEEL IEAYRRKDLD PSVYYWYTEQ RKYGSTPHGG FGLGMERFLV WLLNLDSVRE ACLFPRYMGR CKP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcct ttcgacttat ttttgaacgt cggttggcgc cactctcgaa cagttgtagg cgttgctgcc gcacgatgtt ttctggtccc acgccgcgaa ttacttctgt gacgcgtagt ggactgagag gatatgtatt gtggcaatct ccacctcatg ggagttgcat ctgccctgcg ctttcctctt ctagagcttt tattcactcg ccagaaagga caactatggc ttcttcggag gaattgcttg agagccttca acggattgtt ggcttagatg agaagaactc caaggacctg tctacaaaac cagaacgcgt gcaagacctc cttggcttct ttgcgaatca aaacatgaca ccagagtccc ctcgcgagca aaaagtgatg ctttttaact tgtgggccaa ggttaagctt cccaggcacc gcgatcttct tgcagactac gtgaagagga aaaagctgga ttcaacccaa aaggtggatg ccgccatacg ctttgtggat gccatgaagg aagatgctct ctttgatgct gcaaaatttg aaaaggaatg tggcgtggac gtggttgtct cgaaagatga tacattgcgg ctcgtaaaga cggcattggc agaggaagac ctcaaggcat tgaaaacgtc atgggtgaaa aacccgaaca tgctgcttgg caagatgaag cgggtaccgg gtttgcaatg ggcagacttt gctctggtga aggccatcct tgaggagttg gtaccgccca tgattaagga tgttgtcatt gatgaggctg aacccaagac agaggagaag accacctctc cacctcgcac gaaaggcgct gaagtggcag cgacgcaaaa gtgcccacgc aacgcggatt ggaaatgtgt tgtggatctg cattcactcc atcgcatcgg tgatttatct tctatgcagg aaggtaccaa ggtgcgtgtt gtgggatggg cacaccgagt tcgtcatcaa tcgcgtcttt catttgtggt actgcgtgat ggatctgggt acattcaagc cgtattcaat ggtgaaacag aaccctttca ccgcgaaacg tctttggcaa ttcgcggtac actgaagtac gaacccaagg ctgctactga attgcagcca ccatacgagc ttcatgtgga tgaatacgcc attattggta actcggatgg cagtattgag aacgctatta cccctgaaag ctcccctgat aaattgttgg atgatcgtca tttggtgctt cgtggaacct atggctcaac aaccattaaa gttcgtcacg aattaattcg agcttttcgt gaatactgtt ggtccgtaaa tatgtttgaa gtaacaccac caactcttgt acaggctgag tgtgaagggg gatctacgct ttttgatgtc atttactacg gaacgacggc tcaccttaca cagtcttccc agttatacct tgagacatgc acttcttcag tgggagatgt gtactgtatt cttcccagtt atcgagctga gaaatccaag acgaggcgtc acttgagtga atacacacat ttggaggttg agtacagtat ctgcgacttt gagcttttcc tctcaaaact ggaagatctt attgtgggag cattcaaccg aacaattgag cgggcgggag atttgatcgc ctttatgaat ccggaacagt tggtggatcc aaaggcaaac ccatttgatc ccaacaactg gaagtttgca cccaagaagc cgtttaggcg tctcaagtat gctgacgcca ttaaattatg caatgataac ggcattttga acccagagac aaatgcacca ttccaatttg gtgacgacat cacggatcag ccggagcggg ccatggtggc gatattgggg gagatggtgt tcatgacaca ttttcctgcg gtaatgaagt ctttctacat ggcccgagac ccagaggatc cgacacttac agagtccgtg gacgttttgg ctcctggtat tggggaaatt gtcggcggct ctatgcgtat gtgggatcat gaagaactga tagaggcgta caggcgtaag gaccttgacc caagtgtgta ttattggtac acggagcagc gcaagtatgg ctccacccca catggaggct ttggtttggg aatggagcgc ttcttggtgt ggctgctgaa cttggactcg gtcagggagg catgtctttt cccgcgttac atgggaagat gcaagccgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat