TrcrB.00825.a

aspartyl-tRNA synthetase, putative

CENTER ID: TrcrB.00825.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.00825.a.B1.GE42632 full length 1 564
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.510777.20
UniProt: Q4CNC1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDPDKSNTA ASQEDASGNV ASKNAEKKQA KQNEKEARKA ARLAEENARA AEKAAQLAKY ADLFGAAPLL QSTTYCSKKF SEIYALTKEH VGKTVTVRAR VDTTRKKGKL AFMVLRDGTD SIQAMAAVAE DVPKEMVDFI GQIPCESIVD VEAIVCGVEQ PITSTSQQEI ELKVNKIHVL SEALRVLPFT LEDASRRETD EGIKINFDTR LGCRWLDLRT PASNAIFKVQ SRVGQYFRQF LMDHDFVEIH SPKIIAAASE GGANVFKVGY FNRDAYLAQS PQLYKQMALQ GDLKRVFEVG PVFRAENSNT HRHLTEFVGL DVEMRINEHY YEVLDTAEEL FCYIFAQLAK HTGELEAICR QYPFEPLLWQ LTPEKIKELG VGVIEDSVEP TDIYKARVRN MGTRMLRIHY PHCIALLNTV LEEKLSLTDD INTTNEKLLG KLVKERYGVD FFISDLFPTA ARPFYTMPFP DDERFTNSYD MFIRGEEISS GAQRIHDSSL LLKRAQSLGV DLSPIKDYVD SFRLGAWPHG GFGVGLERVV MLYLGLCNVR LVSLFPRDPQ RVTP
NT Sequence
atgtcggatc ccgataaatc aaacacagca gccagccaag aggacgccag cggcaacgtt gcgtccaaga acgccgaaaa gaaacaggcg aagcagaacg agaaggaagc ccgtaaggcg gcacgcctag cagaggagaa cgctcgtgcg gcggaaaagg cagcccagtt ggcgaaatac gcggatttat ttggtgctgc gccgctgttg cagtcgacca cgtactgctc caagaagttt agtgaaattt atgcactgac caaagagcat gtgggcaaaa cggtgactgt tcgcgctcga gttgacacga cacgcaagaa gggcaaactc gccttcatgg ttctacgcga cggcacagac tccattcaag ctatggcggc tgttgcggag gatgtcccaa aagaaatggt tgattttatc ggacagatcc cttgcgagtc tattgtggac gttgaggcga tcgtgtgcgg tgtggagcaa cccataacct ccacctcgca gcaggaaatt gagctgaagg ttaacaagat tcatgttctg agcgaggcgc tgcgtgtgct tcccttcaca ctggaggatg cgtcgcgccg cgagacagat gagggcatta aaataaactt tgatacccga ctgggctgcc gctggctgga tctgcgcaca ccggcctcaa acgccatatt caaggtgcag tccagggtgg gacaatattt tcgtcaattt ctgatggacc atgactttgt ggagattcat tctccgaaga tcatcgccgc cgcgagcgag ggaggtgcca atgtgttcaa agtgggatac ttcaaccgcg acgcatattt ggcccagtct ccacagctgt ataaacaaat ggcccttcaa ggcgatttga aacgtgtctt tgaagtgggg cctgtattcc gtgcagaaaa cagcaatacc caccgccacc tgacggaatt tgttggccta gatgtggaaa tgcgtataaa tgaacactat tatgaggtac ttgacacggc tgaggagcta ttttgttaca tttttgcaca acttgccaaa catacaggag agcttgaggc gatttgccgt cagtaccctt ttgagccatt gctgtggcaa ttgacaccgg agaaaatcaa agagttgggc gttggcgtca ttgaagactc cgttgagccg accgatattt acaaggctcg tgtacgcaac atgggaactc gtatgttacg catccactac ccgcactgca tcgcactcct gaacaccgtt ctggaggaga agttgtcttt gaccgatgac attaacacca ccaacgagaa attgctcggt aagttggtga aggagcgcta cggcgtggac ttcttcatct ccgatctatt cccaacggct gcacgaccat tctacacgat gccgtttcca gacgatgagc gtttcacgaa ctcgtacgac atgtttatcc gcggtgagga aatatcgagt ggggcgcagc gtattcacga ttcatcactt ttgctgaagc gcgcgcagtc actcggtgtt gatctctcgc ccatcaaaga ctacgtggac tcatttcgtc tcggtgcatg gccccacggc ggatttggcg ttggcctcga gcgtgtggtg atgttatatc ttggcctctg caatgtgcgc ctcgtctcgc tgttcccgcg tgatccgcag cgcgtgacgc cgtga
Details for TrcrB.00825.a.B1.GE42632
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 65kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS DPDKSNTAAS QEDASGNVAS KNAEKKQAKQ NEKEARKAAR LAEENARAAE KAAQLAKYAD LFGAAPLLQS TTYCSKKFSE IYALTKEHVG KTVTVRARVD TTRKKGKLAF MVLRDGTDSI QAMAAVAEDV PKEMVDFIGQ IPCESIVDVE AIVCGVEQPI TSTSQQEIEL KVNKIHVLSE ALRVLPFTLE DASRRETDEG IKINFDTRLG CRWLDLRTPA SNAIFKVQSR VGQYFRQFLM DHDFVEIHSP KIIAAASEGG ANVFKVGYFN RDAYLAQSPQ LYKQMALQGD LKRVFEVGPV FRAENSNTHR HLTEFVGLDV EMRINEHYYE VLDTAEELFC YIFAQLAKHT GELEAICRQY PFEPLLWQLT PEKIKELGVG VIEDSVEPTD IYKARVRNMG TRMLRIHYPH CIALLNTVLE EKLSLTDDIN TTNEKLLGKL VKERYGVDFF ISDLFPTAAR PFYTMPFPDD ERFTNSYDMF IRGEEISSGA QRIHDSSLLL KRAQSLGVDL SPIKDYVDSF RLGAWPHGGF GVGLERVVML YLGLCNVRLV SLFPRDPQRV TP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgg atcccgataa atcaaacaca gcagccagcc aagaggacgc cagcggcaac gttgcgtcca agaacgccga aaagaaacag gcgaagcaga acgagaagga agcccgtaag gcggcacgcc tagcagagga gaacgctcgt gcggcggaaa aggcagccca gttggcgaaa tacgcggatt tatttggtgc tgcgccgctg ttgcagtcga ccacgtactg ctccaagaag tttagtgaaa tttatgcact gaccaaagag catgtgggca aaacggtgac tgttcgcgct cgagttgaca cgacacgcaa gaagggcaaa ctcgccttca tggttctacg cgacggcaca gactccattc aagctatggc ggctgttgcg gaggatgtcc caaaagaaat ggttgatttt atcggacaga tcccttgcga gtctattgtg gacgttgagg cgatcgtgtg cggtgtggag caacccataa cctccacctc gcagcaggaa attgagctga aggttaacaa gattcatgtt ctgagcgagg cgctgcgtgt gcttcccttc acactggagg atgcgtcgcg ccgcgagaca gatgagggca ttaaaataaa ctttgatacc cgactgggct gccgctggct ggatctgcgc acaccggcct caaacgccat attcaaggtg cagtccaggg tgggacaata ttttcgtcaa tttctgatgg accatgactt tgtggagatt cattctccga agatcatcgc cgccgcgagc gagggaggtg ccaatgtgtt caaagtggga tacttcaacc gcgacgcata tttggcccag tctccacagc tgtataaaca aatggccctt caaggcgatt tgaaacgtgt ctttgaagtg gggcctgtat tccgtgcaga aaacagcaat acccaccgcc acctgacgga atttgttggc ctagatgtgg aaatgcgtat aaatgaacac tattatgagg tacttgacac ggctgaggag ctattttgtt acatttttgc acaacttgcc aaacatacag gagagcttga ggcgatttgc cgtcagtacc cttttgagcc attgctgtgg caattgacac cggagaaaat caaagagttg ggcgttggcg tcattgaaga ctccgttgag ccgaccgata tttacaaggc tcgtgtacgc aacatgggaa ctcgtatgtt acgcatccac tacccgcact gcatcgcact cctgaacacc gttctggagg agaagttgtc tttgaccgat gacattaaca ccaccaacga gaaattgctc ggtaagttgg tgaaggagcg ctacggcgtg gacttcttca tctccgatct attcccaacg gctgcacgac cattctacac gatgccgttt ccagacgatg agcgtttcac gaactcgtac gacatgttta tccgcggtga ggaaatatcg agtggggcgc agcgtattca cgattcatca cttttgctga agcgcgcgca gtcactcggt gttgatctct cgcccatcaa agactacgtg gactcatttc gtctcggtgc atggccccac ggcggatttg gcgttggcct cgagcgtgtg gtgatgttat atcttggcct ctgcaatgtg cgcctcgtct cgctgttccc gcgtgatccg cagcgcgtga cgccgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat